Method for reprogramming somatic cells to pancreatic beta cells

ABSTRACT

The present invention relates to a method for reprogramming a somatic cell (e.g. a pancreatic ductal cell, a skin fibroblast or a keratinocyte) to a pancreatic β-cell. This method involves decreasing expression or activity of Fbw7 and/or decreasing phosphorylation-mediated degradation of NGN3 in the somatic cell. The method may be carried out ex vivo or in vivo. The invention also relates to a method of treating a subject with a metabolic disorder (e.g. diabetes) or a subject at risk of a metabolic disorder by administering somatic cells that have been re-programmed to pancreatic β-cells using methods described herein to the subject. In addition, the invention relates to screening methods for identifying a candidate agent for reprogramming a somatic cell to a pancreatic β-cell.

The present invention relates to a method for reprogramming a somatic cell, such as a pancreatic ductal cell, to a pancreatic β-cell. This method may be carried out ex vivo or in vivo. This method has therapeutic applications for the treatment of a subject with a metabolic disorder, such as diabetes, or a subject at risk of developing a metabolic disorder, such as diabetes.

The generation of specific therapeutically relevant cell types is a pre-requisite for cell replacement therapies and an important goal of personalized medicine. Pancreatic β-cells control glucose homeostasis through the production of insulin, and inadequate numbers of insulin-producing β-cells is the major cause of type 1 diabetes mellitus (reviewed in ¹). Therefore the replenishment of lost β-cells may allow for improved diabetes treatment².

The pancreas is composed of an exocrine (ductal and acinar cells) and an endocrine (β-cells, α-cells, δ-cells and pp cells) part, which are distinctively differentiated to secrete enzymes or hormones respectively⁵. Importantly, β-cells are not regenerated during adulthood. β-cell neogenesis in the adult has only been achieved experimentally with rather artificial approaches. For instance, pancreatic duct ligation⁶ and pancreatectomy⁷ experimentally induce adult β-cell neogenesis. Additionally, conversion of other cell types into β-cells by genetic approaches has been demonstrated. Adenoviral overexpression of the three transcription factors Ngn3, Maf1a and Pdx1 is sufficient to convert adult acinar cells into β-cells⁸, and extreme β-cell loss after diphtheria toxin overexpression or overexpression of Pax4 converts glucagon-producing cells into β-cells^(9,10). However, the regulation and mechanisms of β-cell neogenesis in the normal adult pancreas remains largely unknown.

Fbw7 is the substrate recognition component of an SCF-type E3 ubiquitin ligase and can regulate the abundance of several transcription factor substrates involved in the control of progenitor cell fate decisions such as Notch1, c-Myc and c-Jun^(3,4). Fbw7 is frequently inactivated by deletion, mutation or promoter hypermethylation in cancer²⁶. Fbw7 mutations occur in approximately 30% of T-cell acute lymphoblastic leukaemia (T-ALL), 16% of primary endometrial cancer and 11% of colorectal cancer. Overall, approximately 6% of all human primary tumours have mutations in Fbw7²⁷.

The present inventors have found that inactivation of fbw7 reprograms differentiated pancreatic ductal cells in adult mice into cells that show hallmarks of β-cells. The induced β-cells express proteins essential for β-cell function and are indistinguishable from islet β-cells in histological morphology and ultrastructure. Ductal cell to β-cell trans-differentiation occurred rapidly within several days, did not require cellular proliferation, and involved an intermediate stage when cells undergoing reprogramming co-expressed ductal and β-cell markers. Thus, inactivation of fbw7 induces direct cell reprogramming of exocrine ductal cells into β-cells without reversion to a progenitor cell state. Our study highlights the plasticity of seemingly differentiated adult cells, and identifies Fbw7 as a master regulator of cell fate decisions in the adult pancreas.

In a first aspect, the invention provides a method for reprogramming a somatic cell to a pancreatic β-cell, the method comprising the step of decreasing expression or activity of Fbw7 in the somatic cell. The somatic cell may, for example, be a pancreatic ductal cell, a skin fibroblast or a keratinocyte. Preferably, the somatic cell is a pancreatic ductal cell. The method may be carried out ex vivo or in vivo. For example, the method may be carried out in a subject with a metabolic disorder, such as diabetes, or in a subject at risk of developing a metabolic disorder, such as diabetes. The method may comprise contacting the somatic cell, e.g. pancreatic ductal cell, with an agent that reduces the expression or activity of Fbw7 in the somatic cell. The activity of Fbw7 may be the phosphorylation-mediated degradation of neurogenin-3 (NGN3). For example, the agent that reduces the expression or activity of Fbw7 may inhibit the phosphorylation of NGN3 by GSK3β.

In a second aspect, the invention provides a method for reprogramming a somatic cell to a pancreatic β-cell, the method comprising the step of decreasing phosphorylation-mediated degradation of NGN3. The somatic cell may, for example, be a pancreatic ductal cell, a skin fibroblast or a keratinocyte. Preferably, the somatic cell is a pancreatic ductal cell. In one embodiment, the method may comprise the step of decreasing phosphorylation of NGN3 by GSK3β. The method may be carried out ex vivo or in vivo. For example, the method may be carried out in a subject with a metabolic disorder, such as diabetes, or in a subject at risk of developing a metabolic disorder, such as diabetes. The method may comprise the step of contacting the somatic cell, e.g. pancreatic ductal cell, with an agent that decreases the phosphorylation of NGN3 by GSK3β, such as an inhibitor of GSK3β kinase activity.

In a third aspect, the invention provides an agent that reduces the expression or activity of Fbw7 for use in a method of reprogramming a somatic cell to a pancreatic β-cell, the method comprising the step of contacting the somatic cell with said agent. The somatic cell may, for example, be a pancreatic ductal cell, a skin fibroblast or a keratinocyte. Preferably, the somatic cell is a pancreatic ductal cell. The activity of Fbw7 may be the phosphorylation-mediated degradation of NGN3. For example, the agent may inhibit the phosphorylation of NGN3 by GSK3β.

In a fourth aspect, the invention provides an agent that decreases the phosphorylation-mediated degradation of NGN3 for use in a method of reprogramming a somatic cell to a pancreatic β-cell, the method comprising the step of contacting the somatic cell with said agent. The somatic cell may, for example, be a pancreatic ductal cell, a skin fibroblast or a keratinocyte. Preferably, the somatic cell is a pancreatic ductal cell. The agent may inhibit the phosphorylation of NGN3 by GSK3β. For example, the agent may be an inhibitor of GSK3β kinase activity.

In a fifth aspect, the invention provides a method of treating a subject with a metabolic disorder or a subject at risk of developing a metabolic disorder, the method comprising the step of administering to the subject pancreatic β-cells obtained by the method of the first or second aspect above. This method of treating a subject may include the step of reprogramming a somatic cell, such as a pancreatic ductal cell, to a pancreatic β-cell using the method of the first or second aspect above followed by the step of administering the pancreatic β-cells obtained by this method to the subject. The metabolic disorder is preferably diabetes.

In a sixth aspect, the invention provides pancreatic β-cells obtained by the method of the first or second aspect above for use in a method for treatment of a subject with a metabolic disorder or a subject at risk of developing a metabolic disorder, said method comprising administering said pancreatic β-cells to the subject. The metabolic disorder is preferably diabetes.

In a seventh aspect, the invention provides a screening method for identifying a candidate agent for reprogramming a somatic cell, such as a pancreatic ductal cell, to a pancreatic β-cell, the method comprising determining the ability of the agent to reduce expression or activity of Fbw7, wherein an agent that reduces expression or activity of Fbw7 is likely to be able to reprogram a somatic cell, such as a pancreatic ductal cell, to a pancreatic β-cell. The activity of Fbw7 may be the phosphorylation-mediated degradation of NGN3, for example the phosphorylation of NGN3 by GSK3β.

In an eighth aspect, the invention provides a screening method for identifying a candidate agent for reprogramming a somatic cell, such as a pancreatic ductal cell, to a pancreatic β-cell, the method comprising determining the ability of the agent to decrease phosphorylation-mediated degradation of NGN3, wherein an agent that decreases phosphorylation-mediated degradation of NGN3 is likely to be able to reprogram a somatic cell, such as a pancreatic ductal cell, to a pancreatic β-cell. The method may comprise the step of determining the ability of the agent to decrease phosphorylation of NGN3 by GSK3β, wherein an agent that decreases phosphorylation of NGN3 by GSK3β is likely to be able to reprogram a somatic cell, such as a pancreatic ductal cell, to a pancreatic β-cell.

These and other aspects of the invention are described in further detail below.

BRIEF DESCRIPTION OF THE FIGURES

FIG. 1 shows that Pdx1 driven deletion of fbw7 in the pancreas induces occurrence of β-cells in the ducts containing functional and ultrastructural mature β-cell hallmarks (a-d) H&E staining of fbw7^(f/f) or Pdx1-Cre; fbw7^(f/f) pancreas. Numbered squares indicate fbw7^(f/f) pancreatic islet (1) or duct (2) or Pdx1-Cre; fbw7^(f/f) cells with altered morphology in the ducts (3,4). (e-g) Double immunofluorescence (IF) for glut2 and insulin (ins) in 4 week old fbw7^(f/f) (e) or Pdx1-Cre; fbw7^(f/f) (f-g) pancreas. Nuclei were counterstained with DAPI. Ducts are circled with a dashed line. Dashed square represents the area of the magnified picture (g). (h-j) Transmission electron micrograph of fbw7^(f/f) duct (h), fbw7^(f/f) islet β-cell (i) and an induced β-cell situated between two ductal cells in Pdx1-Cre; fbw7^(f/f) pancreas (j). Insets display a high magnification of the insulin granules. White arrows mark ductal cells, white asterisks mark β-cells, black asterisks mark duct lumen.

FIG. 2 shows that ductal β-cells formed after fbw7 deletion co-express different cell lineage markers. (a-b) Double immunofluorescence of fbw7^(f/f) (a) or Pdx1-Cre; fbw7^(f/f) (b) pancreas (4 weeks of age) for insulin (ins) and cytokeratin 19 (ck19). Nuclei were counterstained with DAPI. White dashed square represents the area of the adjacent magnified picture. (c-e) Triple immunofluorescence for glucagon (gluc), amylase (amy), and insulin (ins) in 4 week old fbw7^(f/f) (c) or Pdx1-Cre; fbw7^(f/f) (d-e) pancreas. Nuclei were counterstained with DAPI. White dashed square represents the area of the magnified picture.

FIG. 3 shows that inducible deletion of fbw7 in the adult pancreas promotes β-cell neogenesis from the duct. (a) Scheme of the mouse model. (b) Schematic diagram of RY pancreas before and after tamoxifen injection. (c) GFP immunoperoxidase staining in RY pancreas after tamoxifen injection. Section shows acinar cells, islet and a duct. (d) Quantification of GFP positive cells in the different pancreatic cell type compartments (A: acinar; I: Islet; D: Ducts) RY 13 days post-injection. (e-n) Triple immunofluorescence for insulin (ins), GFP (GFP), amylase (amy) in RY and RFY mice 13 days after tamoxifen injection. Nuclei were counterstained with DAPI. Ducts circled with a white line. White square represents the area of the magnified picture. (o) Western blot analysis of fbw7^(f/f) and Pdx1-Cre; fbw7^(f/f) pancreas lysates for Notch intracellular domain-1 (NICD1), phosphorylated c-jun at ser73 (p-c-jun^(ser73)), phosphorylated c-myc (p-c-myc) and phosphorylated cyclin-E (p-cyclin-E). (p-u) Double immunofluorescence of c-jun and p-c-jun^(ser73) in pancreas from fbw7^(f/f) and Pdx1-Cre; fbw7^(f/f) mice (4 weeks old). Ducts are circled with a white line.

FIG. 4 shows the results of intrapancreatic injection of 4-OH-tamoxifen in RY and RFY mice. (a) Schematic diagram of the experimental strategy. (b,d) GFP immuhistochemistry in RY 5 days (b) or 21 days (d) post-sugery. (c,e) GFP immuhistochemistry in RFY 5 days (c) or 21 days (e) post-surgery. (f,g) Quantification of GFP positive cells in the different pancreatic cell type compartments (A: acinar; I: Islet; D: Ducts) in RY and RFY 5 days (f) or 21 days (g) post-surgery. (h,i) Insulin immunostaining in RY and RFY 21 days post-surgery.

FIG. 5 shows intraperitoneal injection of tamoxifen in RY or RFY mice (a) Schematic diagram of the experimental strategy. (b-d) H&E staining of RY or RFY pancreas 12 weeks post tamoxifen injection. Arrows indicate altered morphology cells in the RFY ducts. (e-g) Insulin immunohistochemistry of RY or RFY pancreas 12 weeks post tamoxifen injection. Arrows indicate insulin positive cells in RFY ducts.

FIG. 6 Shows NGN3 phosphorylation is required for its interaction with Fbw7 and for its stability. (A) Schematic representation of the proposed mechanism of NGN3 degradation by Fbw7. (B) Western Blot analysis of overexpressed NGN3-HA in 293T cells upon GSK3 inhibitor. (C) Schematic representation of NGN3 GSK3 phosphorylation residues and the different NGN3 phospho-mutant construct generated. (D) NGN3-HA/Fbw7-Flag Immunoprecipitation assay using the different NGN3 constructs indicated in C. (E) Western Blot analysis of NGN3 in cells overexpressing the different NGN3 construct indicated in C.

FIG. 7 shows the proposed mechanism of action of Fbw7 in pancreatic progenitors. (A) In wild type situation, Fbw7 would promote the degradation of NGN3. (B) In the absence of Fbw7, the levels of NGN3 would increase.

FIG. 8 shows the proposed mechanism of action in a NGN3-AA conditional transgenic mouse. (A) In a wild type mouse NGN3 will be degraded normally by Fbw7 and the cells will remain as a progenitor status (B) In conditional NGN3-AA transgenic mouse, NGN3 will accumulate and induce differentiation of insulin producing cells.

FIG. 9 shows a schematic of the NGN3 and NGN3-AA constructs used to generate the conditional transgenic mice. (A) Schematic of the NGN3 and NGN3-AA intermediate construct (pDEST). (B) Western blot analysis of NGN3, HA, Flag and Myc in 293T transfected with pDEST-NGN3 or pDEST-NGN3-AA. (C) Schematic of the NGN3 and NGN3-AA constructs used to generate the conditional transgenic mice. (D) DNA sequencing of NGN3 and NGN3-AA constructs used to generate the conditional transgenic mice showing the phosphorylation sites.

FIG. 10 shows PCR analysis of unrecombined and recombined R26-LSL-NGN3-AA in Pdx1-Cre; R26-LSL-NGN3-AA double transgenic mouse. (A) Schematic representation of the locus before and after Cre-mediated recombination. The primers used in the PCR are indicated with arrows. (B) PCR in genomic DNA from tail, liver and pancreas using primers 1,2 and 3. R26-Δ-NGN3-AA: recombined allele; R26-LSL-NGN3: unrecombined allele.

FIG. 11 shows expression of NGN3-AA in the pancreas of the NGN3-AA transgenic mouse. Western blot analysis of NGN3 in Pancreas and liver lysates from Pdx1-Cre; R26-LSL-NGN3-AA and R26-LSL-NGN3-AA mouse.

FIG. 12 shows Q-PCR results for different genes involved in beta cell differentiation in Pdx1-Cre; R26-LSL-NGN3-AA and R26-LSL-NGN3-AA mouse liver and pancreas. (A) Ins1 (B) neurod1 (C) pdx1 (D) ins2 (E) ngn3 (F) hes1.

FIG. 13 shows a low magnification of insulin immunostaining in (A) Pdx1-Cre; R26-LSL-NGN3-AA and (B) R26-LSL-NGN3-AA pancreas.

FIG. 14 shows pancreatic spheres generated in Fbw7+/+; Rosa-CreERT and Fbw7 F/F; Rosa-CreERT mice injected with tamoxifen. (A) Day 4 after culture (B) Day 15 after culture.

FIG. 15 shows YFP fluorescence of pancreatic spheres generated in Fbw7+/+; Rosa-CreERT and Fbw7 F/F; Rosa-CreERT mice injected with tamoxifen after 14 days in 24 well adherent plates (A) Bright field (B) YFP fluorescence (C) High magnification of spheres.

FIG. 16 shows pancreatic spheres and differentiated cells generated in Fbw7+/+; Rosa-CreERT and Fbw7 F/F; Rosa-CreERT mice injected with tamoxifen after dissociation of day 4 spheres (A) Fbw7+/+; Rosa-CreERT (B) Fbw7 F/F; Rosa-CreERT low and high mag of spheres and differentiated cells.

FIG. 17 shows insulin and CK19 staining in Fbw7 F/F; Rosa-CreERT differentiated cells. (A) Bright field and Insulin immunofluorescence. (B) Insulin/DAPI and (C) CK19/DAPI staining.

FIG. 18 shows that converted β-cells induced by fbw7 deletion show hallmarks of functional mature β-cells. (a) Schematic diagram of the comparison strategy used for the mRNA expression profile. Heat map representation of 15 representative β-cell markers in GFP+ sorted cells from tamoxifen injected CY, CFY and MIP-GFP mice. (b) qPCR analysis of ins2, gck, pdx1 and nkx6.1 in GFP+ cells sorted from CY and CFY cells. (c) Double immunofluorescence of ductal CFY β-cells for insulin (ins) together with different β-cell markers (c-PPT, glut2, MafA1, Nkx6.1, Pax6, PC1/3, Pdx1) or ductal markers (Sox9, DBA) (scale bar: 5 μm). (d) Schematic diagram of the glucose challenge experiment design and FACS profile in uncompensated signal, indicating the sorting gating used for the isolation of the GFP+ cells in CY and CFY mice. ELISA for insulin levels in the supernatant from GFP+ cells from CY and CFY 0, 15, 30 and 60 min after the addition of 20 mM glucose.

FIG. 19 shows the blood glucose level in Pdx1-Cre, R26-LSL-NGN3-AA and R26-LSL-NGN3-AA mice after intraperitoneal injection of glucose (2 g/kg bw).

DETAILED DESCRIPTION

The present invention is based on the finding that inactivation of fbw7 reprograms differentiated somatic cells, e.g. pancreatic ductal cells, into cells that show hallmarks of pancreatic β-cells. The invention is also based on the identification of NGN3 as a novel substrate for Fbw7 and the finding that Fbw7 targets NGN3 for ubiquitin-mediated destruction by the proteasome.

In a first aspect, the invention provides a method for reprogramming a somatic cell to a pancreatic β-cell, the method comprising the step of decreasing expression or activity of Fbw7 in the somatic cell. The somatic cell may, for example, be a pancreatic ductal cell, a skin fibroblast or a keratinocyte. Preferably, the somatic cell is a pancreatic ductal cell. The method may be carried out ex vivo or in vivo. For example, the method may be carried out in a subject with a metabolic disorder, such as diabetes, or in a subject at risk of developing a metabolic disorder, such as diabetes. Alternatively, the method may be carried out in an experimental animal, such as a mouse or rat.

The method may comprise contacting the somatic cell, such as a pancreatic ductal cell, with an agent that reduces the expression or activity of Fbw7 in the somatic cell. The activity of Fbw7 may be the phosphorylation-mediated degradation of NGN3. For example, the agent that reduces the expression or activity of Fbw7 may inhibit the phosphorylation of NGN3 by GSK3β.

Pancreatic ductal cells form the epithelial lining of the branched tubes that deliver enzymes produced by pancreatic acinar cells into the duodenum. In addition, these cells secrete bicarbonate that neutralizes stomach acidity. During development, epithelium of endodermal origin evaginates from the future duodenum area and invades the mesenchyme to form a complex branched network. All endocrine, acinar and ductal cells arise from common precursors in this epithelial structure. Adult ductal cells share some similarities with embryonic primitive ducts and may retain the ability to generate endocrine cells in the adult²⁸. Pancreatic ductal cells may, for example, be obtained from pancreatic biopsies, e.g. from a human pancreatic biopsy, or from a full post-mortem pancreas in the case of an experimental animal. Pancreatic tissue may, for example, be digested using collagenase and single cell suspensions of pancreatic ductal cells cultured as described in detail below.

The pancreatic ductal cells have preferably been obtained from a subject with a metabolic disorder, such as diabetes, or from a subject at risk of developing a metabolic disorder, such as diabetes. For example, these cells may have been obtained from a pancreatic biopsy taken from a subject with a metabolic disorder, such as diabetes, or from a subject at risk of developing a metabolic disorder, such as diabetes. The subject is preferably a mammal, and most preferably a human.

Fbw7 is an ubiquitin E3 ligase substrate adaptor. Ubiquitin E3 ligases can physically bind to and add ubiquitin chains to a target protein to result in its recognition and degradation in an ATP-dependent manner by the proteasome. Fbw7 (or FBW7) is also known as F-box/WD repeat-containing protein 7 isoform 1, CDC4, Ago (or AGO), hSel10, Fbxw7, FLJ16457, FBW6, SEL-10, CDC4, DKFZp686F23254, FBX30, FBXO30, FBXW6, or SEL1.

The amino acid and nucleotide sequences of human and mouse Fbw7 are shown in Tables 1, 2a and 2b below.

When carried out ex vivo, the method may comprise the step of decreasing expression or activity of Fbw7 in a somatic cell, such as a pancreatic ductal cell, obtained from a subject with a metabolic disorder, such as diabetes, or from a subject at risk of developing a metabolic disorder, such as diabetes. The method may comprise contacting the somatic cell, such as a pancreatic ductal cell, with an agent that reduces the expression or activity of Fbw7 in the somatic cell. The activity of Fbw7 may be the phosphorylation-mediated degradation of NGN3. For example, the agent that reduces the expression or activity of Fbw7 may inhibit the phosphorylation of NGN3 by GSK3β.

When carried out in vivo, the method may comprise the step of decreasing expression or activity of Fbw7 in a somatic cell, such as a pancreatic ductal cell, present in a subject with a metabolic disorder, such as diabetes, in a subject at risk of developing a metabolic disorder, such as diabetes, or in an experimental animal, such as a mouse or a rat. For example, the method may comprise the step of administering to the subject or experimental animal an agent that reduces the expression or activity of Fbw7 in a somatic cell, such as a pancreatic ductal cell. The activity of Fbw7 may be the phosphorylation-mediated degradation of NGN3. For example, the agent that reduces the expression or activity of Fbw7 may inhibit the phosphorylation of NGN3 by GSK3β. The subject is preferably a mammal, and most preferably a human.

The agent that reduces the expression or activity of Fbw7 may be administered to the subject or experimental animal by any suitable route of administration. For example, the agent may be administered by intraductal, intraperitoneal or intravenous injection or orally.

Decreased or reduced expression of Fbw7 may be achieved at the nucleic acid level or at the protein level. For example, the transcription of the Fbw7 gene into Fbw7 mRNA may be decreased or reduced, or the translation of Fbw7 mRNA into Fbw7 protein may be decreased or reduced.

Alternatively, decreased or reduced Fbw7 expression or activity may be achieved by decreasing or reducing Fbw7 protein stability or by inhibiting Fbw7 dimerisation.

The activity of Fbw7 includes its downstream effects on its substrates such as c-Myc, Cyclin E, c-jun, c-Myb, KLF5, Mcl-1, mTOR, Notch1, SREBP1, presenilin and Notch intracellular domain 1 (NICD1). The inventors have also identified NGN3 as a novel substrate for Fbw7 and have found that Fbw7 targets NGN3 for ubiquitin-mediated destruction by the proteasome, thus reducing its level in cells. Specifically, Fbw7 recognises phosphorylated NGN3 and targets it for ubiquitin-mediated destruction. Therefore, reducing the activity of Fbw7 may include reducing its downstream activity, e.g. by reducing ubiquitin-mediated destruction of NGN3 by Fbw7. For example, reducing the activity of Fbw7 may include reducing the phosphorylation of NGN3. This reduces its targeting for ubiquitin-mediated destruction by Fbw7, thus reducing the downstream activity of Fbw7. For example, the agent that reduces the expression or activity of Fbw7 may inhibit the phosphorylation of NGN3, e.g. by GSK3β. Therefore, the agent that reduces the expression or activity of Fbw7 may be an inhibitor of GSK3β. Examples of agents that reduces the expression or activity of Fbw7 by inhibiting the phosphorylation of NGN3 by GSK3β include lithium chloride, CHIR-99021 (CT99021), SB 216763, TWS119, CHIR-98014, SB 415286, tideglusib, LY2090314 and TDZD-8.

NGN3 (neurogenin-3) is a regulator of pancreatic islet differentiation and regeneration²⁹. NGN3 is also known as Neurog3, Atoh5, MGC129292, MGC129293, Math4B, bHLHa7, Atonal protein homolog 5, Helix-loop-helix protein mATH-4B, MATH4B), atonal homolog 5, protein atonal homolog 5.

The amino acid and nucleotide sequences of human and mouse NGN3 are shown in Tables 3 and 4 below.

The agent that reduces the expression or activity of Fbw7 for use in the invention may, for example, be a small molecule, a nucleotide sequence, a protein (e.g. an antibody), a peptide, a modified peptide (e.g. a stapled peptide), an aptamer or an RNA interference (RNAi) agent (e.g. an siRNA). For example, the agent may be a small molecule inhibitor of NGN3 phosphorylation by GSK3β (as discussed above), e.g. lithium chloride, CHIR-99021 (CT99021), SB 216763, TWS119, CHIR-98014, SB 415286, tideglusib, LY2090314 or TDZD-8; an anti-Fbw7 antibody; or an siRNA molecule that reduces the expression of Fbw7. The agent may also reduce or decrease the stability of Fbw7 or inhibit its dimerization. Exemplary siRNA sequences that can be used to reduce the expression of Fbw7 include:

(a) 5′-cttttggggacctcagagc-3′ (b) 5′-ccaacacaagtaaaacata-3′ (c) 5′-ccttctctggagagagaaa-3′ (d) 5′-agaaaccatgcaaagtctc-3′ (e) 5′-caagaactttgtaattacc-3′

As mentioned above and as demonstrated by the Examples below, the inventors have identified NGN3 as a novel substrate for Fbw7 and have found that Fbw7 targets NGN3 for ubiquitin-mediated destruction by the proteasome, thus reducing its level in cells. Specifically, Fbw7 recognises phosphorylated NGN3 and targets it for ubiquitin-mediated destruction (see FIGS. 7 and 8).

Therefore, in a second aspect, the invention provides a method for reprogramming a pancreatic ductal cell to a pancreatic β-cell, the method comprising the step of decreasing phosphorylation-mediated degradation of NGN3. For example, the method may comprise the step of decreasing phosphorylation of NGN3 by GSK3β. The method may be carried out ex vivo or in vivo. For example, the method may be carried out in a subject with a metabolic disorder, such as diabetes, in a subject at risk of developing a metabolic disorder, such as diabetes, or in an experimental animal. The method may comprise the step of contacting the somatic cell, such as a pancreatic ductal cell, with an agent that decreases the phosphorylation of NGN3 by GSK3β, such as an inhibitor of GSK3β kinase activity.

The pancreatic ductal cells have preferably been obtained from a subject with a metabolic disorder, such as diabetes, or from a subject at risk of developing a metabolic disorder, such as diabetes. For example, these cells may have been obtained from a pancreatic biopsy taken from a subject with a metabolic disorder, such as diabetes, or from a subject at risk of developing a metabolic disorder, such as diabetes. The subject is preferably a mammal, and most preferably a human.

When carried out ex vivo, the method may comprise the step of decreasing phosphorylation-mediated degradation of NGN3 in a somatic cell, such as a pancreatic ductal cell, obtained from a subject with a metabolic disorder, such as diabetes, or from a subject at risk of developing a metabolic disorder, such as diabetes. The method may comprise contacting the somatic cell, such as a pancreatic ductal cell, with an agent that decreases the phosphorylation-mediated degradation of NGN3 in the somatic cell. For example, the agent that decreases phosphorylation-mediated degradation of NGN3 may inhibit phosphorylation of NGN3 by GSK3β. The subject is preferably a mammal, and most preferably a human.

When carried out in vivo, the method may comprise the step of decreasing phosphorylation-mediated degradation of NGN3 in a somatic cell, such as a pancreatic ductal cell, present in a subject with a metabolic disorder, such as diabetes, in a subject at risk of developing a metabolic disorder, such as diabetes, or in an experimental animal, such as a mouse or rat. For example, the method may comprise the step of administering to the subject or experimental animal an agent that decreases the phosphorylation-mediated degradation of NGN3 in the somatic cell, such as a pancreatic ductal cell. For example, the agent that decreases phosphorylation-mediated degradation of NGN3 may inhibit phosphorylation of NGN3 by GSK3β. The subject is preferably a mammal, and most preferably a human.

The agent that decreases the phosphorylation-mediated degradation of NGN3 for use in the invention may, for example, be a small molecule, a nucleotide sequence, a protein (e.g. an antibody), a peptide, a modified peptide (e.g. a stapled peptide), an aptamer or an RNA interference (RNAi) agent (e.g. an siRNA). For example, the agent may be a small molecule inhibitor of NGN3 phosphorylation by GSK3β (e.g. lithium chloride, CHIR-99021 (CT99021), SB 216763, TWS119, CHIR-98014, SB 415286, tideglusib, LY2090314 or TDZD-8), an anti-GSK3β antibody or an siRNA molecule that reduces the expression of GSK3β.

In a third aspect, the invention provides an agent that reduces the expression or activity of Fbw7 for use in a method of reprogramming a somatic cell to a pancreatic β-cell, the method comprising the step of contacting the somatic cell with said agent. For example, the somatic cell may be a pancreatic ductal cell, a skin fibroblast or a keratinocyte. The somatic cell is preferably a pancreatic ductal cell. The activity of Fbw7 may be the phosphorylation-mediated degradation of NGN3. For example, the agent may inhibit the phosphorylation of NGN3 by GSK3β.

The pancreatic ductal cells have preferably been obtained from a subject with a metabolic disorder, such as diabetes, or from a subject at risk of developing a metabolic disorder, such as diabetes. For example, these cells may have been obtained from a pancreatic biopsy taken from a subject with a metabolic disorder, such as diabetes, or from a subject at risk of developing a metabolic disorder, such as diabetes. The subject is preferably a mammal, and most preferably a human.

As set out above, Fbw7 (or FBW7) is also known as F-box/WD repeat-containing protein 7 isoform 1, CDC4, Ago (or AGO), hSel10, Fbxw7, FLJ16457, FBW6, SEL-10, CDC4, DKFZp686F23254, FBX30, FBXO30, FBXW6, or SEL1. The amino acid and nucleotide sequences of human and mouse Fbw7 are shown in Table 1.

The method of reprogramming the somatic cell, such as a pancreatic ductal cell, may be carried out ex vivo or in vivo. When the method is carried out ex vivo, the method may include the step of obtaining a somatic cell, such as a pancreatic ductal cell, from a subject with a metabolic disorder, such as diabetes, or from a subject at risk of developing a metabolic disorder, such as diabetes, and then contacting the somatic cell, such as a pancreatic ductal cell, with said agent ex vivo. When the method is carried out in vivo, the method may include the step of contacting a somatic cell, such as a pancreatic ductal cell, present in a subject with a metabolic disorder, such as diabetes, or in a subject at risk of developing a metabolic disorder, such as diabetes, with said agent. For example, the method may include administering said agent to a subject with a metabolic disorder, such as diabetes, or in a subject at risk of developing a metabolic disorder, such as diabetes. The subject is preferably a mammal, and most preferably a human.

Decreased or reduced expression of Fbw7 may be achieved at the nucleic acid level or at the protein level. For example, transcription of the Fbw7 gene into Fbw7 mRNA may be decreased or reduced, or translation of Fbw7 mRNA into Fbw7 protein may be decreased or reduced.

Alternatively, decreased or reduced Fbw7 expression or activity may be achieved by decreasing or reducing Fbw7 protein stability or by inhibiting Fbw7 dimerisation.

The activity of Fbw7 includes its downstream effects on its substrates such as c-Myc, Cyclin E, c-jun, c-Myb, KLF5, Mcl-1, mTOR, Notch1, SREBP1 and presenilin. As described herein, the inventors have also identified NGN3 as a novel substrate for Fbw7 and have found that Fbw7 targets NGN3 for ubiquitin-mediated destruction by the proteasome, thus reducing its level in cells. Specifically, Fbw7 recognises phosphorylated NGN3 and targets it for ubiquitin-mediated destruction. Therefore, reducing the activity of Fbw7 includes reducing its downstream activity, e.g. by reducing ubiquitin-mediated destruction of NGN3 by Fbw7. For example, reducing the activity of Fbw7 may include reducing the phosphorylation of NGN3. This reduces its targeting for ubiquitin-mediated destruction by Fbw7, thus reducing the downstream activity of Fbw7. For example, the agent that reduces the expression or activity of Fbw7 may inhibit the phosphorylation of NGN3, e.g. by GSK3β. Therefore, the agent that reduces the expression or activity of Fbw7 may be an inhibitor of GSK3β. Examples of agents that reduce the expression or activity of Fbw7 by inhibiting the phosphorylation of NGN3 by GSK3β include lithium chloride, CHIR-99021 (CT99021), SB 216763, TWS119, CHIR-98014, SB 415286, tideglusib, LY2090314 and TDZD-8.

The agent that reduces the expression or activity of Fbw7 for use in the invention may, for example, be a small molecule, a nucleotide sequence, a protein (e.g. an antibody), a peptide, a modified peptide (e.g. a stapled peptide), an aptamer or an RNA interference (RNAi) agent (e.g. an siRNA). For example, the agent may be a small molecule inhibitor of NGN3 phosphorylation by GSK3β (as discussed above), e.g. lithium chloride, CHIR-99021 (CT99021), SB 216763, TWS119, CHIR-98014, SB 415286, tideglusib, LY2090314 or TDZD-8; an anti-Fbw7 antibody; an anti-Fbw7 antibody; or an siRNA molecule that reduces the expression of Fbw7. Exemplary siRNA sequences that can be used to reduce the expression of Fbw7 include:

(a) 5′-cttttggggacctcagagc-3′ (b) 5′-ccaacacaagtaaaacata-3′ (c) 5′-ccttctctggagagagaaa-3′ (d) 5′-agaaaccatgcaaagtctc-3′ (e) 5′-caagaactttgtaattacc-3′

In a fourth aspect, the invention provides an agent that decreases the phosphorylation-mediated degradation of NGN3 for use in a method of reprogramming a pancreatic ductal cell to a pancreatic β-cell, the method comprising the step of contacting the pancreatic ductal cell with said agent. The agent may inhibit the phosphorylation of NGN3 by GSK3β. For example, the agent may be an inhibitor of GSK3β kinase activity.

The somatic cells, such as pancreatic ductal cells, have preferably been obtained from a subject with a metabolic disorder, such as diabetes, or from a subject at risk of developing a metabolic disorder, such as diabetes. For example, these cells may have been obtained from a pancreatic biopsy taken from a subject with a metabolic disorder, such as diabetes, or from a subject at risk of developing a metabolic disorder, such as diabetes.

As set out above, NGN3 is also known as Neurog3, Atoh5, MGC129292, MGC129293, Math4B, bHLHa7, Atonal protein homolog 5, Helix-loop-helix protein mATH-4B, MATH4B), atonal homolog 5, protein atonal homolog 5.

The amino acid and nucleotide sequences of human and mouse NGN3 are shown in Tables 3 and 4.

The method of reprogramming the somatic cell, such as a pancreatic ductal cell, may be carried out ex vivo or in vivo. When the method is carried out ex vivo, the method may include the step of obtaining a somatic cell, such as a pancreatic ductal cell, from a subject with a metabolic disorder, such as diabetes, or from a subject at risk of developing a metabolic disorder, such as diabetes, and then contacting the somatic cell, such as a pancreatic ductal cell, with said agent ex vivo. The subject is preferably a mammal, and most preferably a human. When the method is carried out in vivo, the method may include the step of contacting a somatic cell, such as a pancreatic ductal cell, present in a subject with a metabolic disorder, such as diabetes, or in a subject at risk of developing a metabolic disorder, such as diabetes, with said agent. For example, the method may include administering said agent to a subject with a metabolic disorder, such as diabetes, or in a subject at risk of developing a metabolic disorder, such as diabetes. The subject is preferably a mammal, and most preferably a human.

The agent that decreases the phosphorylation-mediated degradation of NGN3 for use in the invention may, for example, be a small molecule, a nucleotide sequence, a protein (e.g. an antibody), a peptide, a modified peptide (e.g. a stapled peptide), an aptamer or an RNA interference (RNAi) agent (e.g. an siRNA). For example, the agent may be a small molecule inhibitor of NGN3 phosphorylation by GSK3β (as discussed above), e.g. lithium chloride, an anti-GSK3β antibody or an siRNA molecule that reduces the expression of GSK3β. Exemplary siRNA sequences that can be used to reduce the expression of Fbw7 include:

(a) 5′-cttttggggacctcagagc-3′ (b) 5′-ccaacacaagtaaaacata-3′ (c) 5′-ccttctctggagagagaaa-3′ (d) 5′-agaaaccatgcaaagtctc-3′ (e) 5′-caagaactttgtaattacc-3′

In a fifth aspect, the invention provides a method of treating a subject with a metabolic disorder or a subject at risk of developing a metabolic disorder, the method comprising the step of administering to the subject pancreatic β-cells obtained by the method of the first or second aspect above. This method of treating a subject may include the step of reprogramming a somatic cell, such as a pancreatic ductal cell, to a pancreatic β-cell using the method of the first or second aspect above followed by the step of administering the pancreatic β-cells obtained by this method to the subject. The metabolic disorder is preferably diabetes. The subject is preferably a mammalian subject and most preferably a human subject. Pancreatic β-cells may be administered to the subject by any suitable method, such as intra-portal vein (liver) islet transplantation or kidney capsule transplantation in humans, and kidney capsule, intrapancreatic or intrahepatic transplantation in mice.

In a sixth aspect, the invention provides pancreatic β-cells obtained by the method of the first or second aspect above for use in a method for treatment of a subject with a metabolic disorder or a subject at risk of developing a metabolic disorder, said method comprising administering said pancreatic β-cells to the subject. The metabolic disorder is preferably diabetes. The subject is preferably a mammalian subject and most preferably a human subject. Pancreatic β-cells may be administered to the subject by any suitable method, such as intra-portal vein (liver) islet transplantation or kidney capsule transplantation in humans, and kidney capsule, intrapancreatic or intrahepatic transplantation in mice.

In a seventh aspect, the invention provides a screening method for identifying a candidate agent for reprogramming a somatic cell to a pancreatic β-cell, the method comprising determining the ability of the agent to reduce expression or activity of Fbw7, wherein an agent that reduces expression or activity of Fbw7 is likely to be able to reprogram the somatic cell to a pancreatic β-cell. For example, the somatic cell may be a pancreatic ductal cell, a skin fibroblast or a keratinocyte. The somatic cell is preferably a pancreatic ductal cell. The activity of Fbw7 may be the phosphorylation-mediated degradation of NGN3, for example the phosphorylation of NGN3 by GSK33.

The screening method may be carried out in vitro or in vivo.

The candidate agent may reduce expression of Fbw7 at the nucleic acid level or at the protein level. For example, the candidate agent may reduce transcription of the Fbw7 gene into Fbw7 mRNA or the candidate agent may reduce translation of Fbw7 mRNA into Fbw7 protein may be reduced.

The activity of Fbw7 includes its downstream effects on its substrates such as c-Myc, Cyclin E, c-jun, c-Myb, KLF5, Mcl-1, mTOR, Notch1, SREBP1 and presenilin. As described herein, reducing the activity of Fbw7 may include reducing ubiquitin-mediated destruction of NGN3 by Fbw7. For example, reducing the activity of Fbw7 may include reducing its downstream activity, e.g. by reducing the phosphorylation of NGN3. This reduces its targeting for ubiquitin-mediated destruction by Fbw7, thus reducing the downstream activity of Fbw7. For example, the method may comprise determining the ability of the agent to reduce phosphorylation of NGN3 (e.g. by GSK3β), wherein an agent that reduces phosphorylation of NGN3 is likely to be able to reprogram a somatic cell, such as a pancreatic ductal cell, to a pancreatic β-cell.

The method may comprise the step of contacting a cell expressing Fbw7 with the candidate agent and determining the level of Fbw7 mRNA in the cell before and after the contacting step, wherein a decrease in Fbw7 mRNA following the contacting step indicates that the agent is likely to be able to reprogram a somatic cell, such as a pancreatic ductal cell, to a pancreatic β-cell. The level of Fbw7 mRNA may be determined by any suitable method known in the art, such as quantitative PCR.

The method may comprise the step of contacting a cell co-expressing NGN3 and Fbw7 with the candidate agent and determining ubiquitination of NGN3 by Fbw7 in the absence or presence of the agent, wherein a reduction in ubiquitination of NGN3 following the step of contacting the cell with the agent indicated that the agent is likely to be able to reprogram a somatic cell, such as a pancreatic ductal cell, to a pancreatic β-cell.

The method may comprise the step of contacting a cell expressing Fbw7 with the candidate agent and determining the level of Fbw7 protein expression or expression of a substrate of Fbw7, such as NGN3, c-Jun or Notch intracellular domain 1 (NICD-1), in the cell before and after the contacting step, wherein a decrease in Fbw7 protein expression or expression of a substrate of Fbw7 following the contacting step indicates that the agent is likely to be able to reprogram a somatic cell, such as a pancreatic ductal cell, to a pancreatic β-cell. The level of Fbw7 protein or a substrate of FBw7 may be determined by any suitable method known in the art, such as immunohistochemistry.

In an eighth aspect, the invention provides a screening method for identifying a candidate agent for reprogramming a somatic cell to a pancreatic β-cell, the method comprising determining the ability of the agent to decrease phosphorylation-mediated degradation of NGN3, wherein an agent that decreases phosphorylation-mediated degradation of NGN3 is likely to be able to reprogram a somatic cell to a pancreatic β-cell. For example, the somatic cell may be a pancreatic ductal cell, a skin fibroblast or a keratinocyte. The somatic cell is preferably a pancreatic ductal cell. The method may comprise the step of determining the ability of the agent to decrease phosphorylation of NGN3 by GSK3β, wherein an agent that decreases phosphorylation of NGN3 by GSK3β is likely to be able to reprogram a somatic cell, such as a pancreatic ductal cell, to a pancreatic β-cell.

The step of determining whether an agent decreases phosphorylation of NGN3 by GSK3β may be carried out by any suitable method known in the art, such as phospho-specific NGN3 antibody development. Phosphorylation of NGN3 may also be determined indirectly by determining its ability to interact with Fbw7, wherein an agent that decreases the ability of NGN3 to interact with Fbw7 is likely to be able to reprogram a somatic cell, such as a pancreatic ductal cell, to a pancreatic β-cell.

Further aspects and embodiments of the invention will be apparent to those skilled in the art given the present disclosure including the following experimental exemplification.

Experimental Materials and Methods Mouse Lines

The Pdx1-cre²¹, R26-LSL-YFP²², R26-CreERT²³ and Fbw7^(f/f 24) mouse lines have been previously described.

Genetic Labelling Experiments

Mice were either injected intraperitoneally with tamoxifen (TM) or intrapancreatically injected with 4-OH-tamoxifen as indicated. Short and long term histological analyses were performed at short. (5 or 13 days) or long (60 or 82 days) post-injections. Pancreata were excised, fixed in cold 4% paraformaldehyde for 12 h, embedded in paraffin, cut into 7-10 μm sections and staining was performed as described below.

Immunofluorescence Staining

Mice were euthanized by cervical dislocation and pancreas dissected out into ice-cold PBS. The pancreas was fixed overnight in 10% neutral buffered formalin, briefly washed with PBS and transferred into 70% ethanol, processed and embedded into paraffin. Sections were cut at 7 μm for Haematoxylin & Eosin staining.

Immunofluorescence and immunohistochemistry staining was performed as described before¹³ using antibodies against GFP (Roche Applied Science), glut2 (Millipore), insulin (Sigma), amylase (Santa Cruz) and glucagon (Sigma) were used. The percentage of GFP positive cells per cell type (see FIG. 3 and FIG. 6) was determined in at least 5 sections per mouse (number of mice>3 per genotype).

Quantification of number of ducts per area, number of isolated insulin clusters per area, number of insulin clusters in close proximity to a duct per area and number of insulin clusters within a duct (see FIG. 4) was performed on at least 10 sections per mouse (3 mouse per genotype).

Electron Microscopy

EM analysis was done by the Electron Microscopy service at London Research Institute (Cancer Research UK). Briefly, dissected pancreas was fixed in 4% paraformaldehyde and 0.1% glutaraldehyde for 2 h at room temperature (24° C.). For conventional transmission electron microscopy, samples were further fixed by osmium tetroxide, embedded in Epon resin and sectioned at 60-80 nm. Images were obtained with a Tecnai G² Spirit BioTWIN transmission electron microscope.

Western Blot Analysis

Pancreata were dissected out and lysates were homogenized in RIPA lysis buffer supplemented with protease inhibitor (Sigma). Immunoblots were carried out as previously described²⁵. The gels were transferred to nitrocellulose membranes, and the membranes were immunoblotted with various antibodies as indicated: NICD-1 (Abcam), p-c-Junser73 (Santa Cruz), p-Myc (Santa Cruz), p-cyclin E (Santa Cruz) and actin (Sigma).

Statistics

Statistical evaluation was performed by Student's unpaired t test. Data are presented as mean±SEM. **p≦0.001 was considered highly statistically significant; ***p≦0.0001 was considered very highly statistically significant.

Cell Culture and Transfection

HEK293T cells were cultured in DMEM and 10% FBS. Cells were plated at subconfluence and transfected with Lipofectamine Reagent (Invitrogen) following the manufacturer instructions.

Sphere Cultures

Pancreas isolated from the mice with the indicated genotypes was digested in collagenase (1.6 mg/ml—Worthington Biochemical Corporation) at 37° C. during 30 min at constant shaking. Pancreas tissue homogenate after digestion was passed through a Nylon mesh (70 μm). Eluted cells were cultured under self-renewal conditions in Neurobasal Medium (Invitrogen) supplemented with 1% Penicillin/Streptomycin (10000 U/ml; Invitrogen), 1% L-glutamine (200 mM; Invitrogen), 2% B27 supplement (Invitrogen), human epidermal growth factor (EGF; 20 ng/ml; PeproTech) and fibroblast growth factor (FGF-basic; 20 ng/ml; PeproTech). Cells were plated in non-adherent or adherent 24-well plates as indicated. For differentiation, the primary spheres were mechanically dissociated and replated in 24 well plates.

Plasmids

PCR generated NGN3-HA was cloned into pDEST-Flag using Gateway® technology. All the phosphomutants forms were generated by PCR-direct mutagenesis and cloned in pDEST-Flag using Gateway® technology. For the generation of the construct to generate the NGN3-AA transgenic mouse, the PCR product (NGN3-AA) was cloned into pRosa26-DV1 plasmid using Gateway® technology.

Immunoprecipitation

Cells transfected with the indicated plasmids were treated with proteasome inhibitor for 3 hours. Cells were then lysed for 30 min at 4° C. in 500 μl of immunoprecipitation (IP) buffer containing 0.5% Triton X-100, 1 mM EDTA, 100 μM Na-orthovanadate, 0.25 mM PMSF, and protease inhibitor mixture (Sigma) in PBS. After centrifugation, supernatants were incubated for 3 h at 4° C. with 1 μg of the Flag or HA antibody (Sigma) coupled to Protein A-Sepharose beads. IP products and whole lysates were run in a 12% gel, transferred to a nitrocellulose membrane and immunoblotted with anti-HA or anti-Flag antibodies.

Western Blot Analysis

HEK293T cells lysates or tissues were homogenized in RIPA lysis buffer supplemented with protease inhibitor (Sigma). Immunoblots were carried out as previously described⁴. The gels were transferred to nitrocellulose membranes, and the membranes were immunoblotted with various antibodies as indicated: anti-NGN3, anti-flag, anti-HA, and anti-Myc.

Quantitative RT-PCR

For qRT-PCRs, cDNA was synthesised using Invitrogen Superscript reagents according to the manufacturer's instructions. Quantitative real-time PCR was accomplished with SYBRGreen incorporation (Platinum® Quantitative PCR SuperMix-UDG w/ROX, Invitrogen) using an ABI7900HT (Applied Bioscience), and the data were analyzed using the SDS 2.3 software. Results were normalized to those obtained with β-actin and results are presented as fold induction over control mice.

The sequences of the primers used for Q-PCR analysis were:

mHes1-Fw: 5′-TGCCAGCTGATATAATGGAGAA-3′ mHes1-Rv: 5′-CCATGATAGGCTTTGATGACTTT-3′ mIns1-Fw: 5′-CAGAGAGGAGGTACTTTGGACTATAAA-3′ mIns1-Rv: 5′-GCCATGTTGAAACAATGACCT-3′ mIns2-Fw: 5′-GAAGTGGAGGACCCACAAGT-3′ mIns2-Rv: 5′-AGTGCCAAGGTCTGAAGGTC-3′ mPdx1-Fw: 5′-GAAATCCACCAAAGCTCACG-3′ mPdx1-Rv: 5′-CGGGTTCCGCTGTGTAAG-3′ mNeuroD1-Fw: 5′-CCTGCGCTCAGGCAAAA-3′ mNeuroD1-Rv: 5′-GCTGGGACAAACCTTTGCA-3′ mNGN3-B-Fw: 5′-GCGCCTCATCCCTTGGA-3′ mNGN3-B-Rv: 5′-CCGGGAAAAGGTTGTTGTGT-3′

Immunostaining

Mice were euthanised by cervical dislocation and the pancreas was dissected out. The pancreas was fixed overnight in 10% neutral buffered formalin, briefly washed with PBS, transferred into 70% ethanol, processed and embedded into paraffin. Sections were cut at 4 μm for immunohistochemistry. For immunohistochemistry, an antibody against insulin was used.

Immunofluorescence

Sphere derived differentiated cells were washed twice with cold PBS and fixed with 4% PFA at room temperature for 15 minutes. Cells were washed with PBS and permeabilized with PBS+0.1% Triton for 10 min. Cells were washed with PBS and blocked in 1% BSA/PBS before addition of primary antibodies diluted 1:1000 in blocking buffer. Cells were washed three times for 5 minutes with blocking buffer followed by the addition of secondary antibodies diluted 1:400 in blocking buffer for 1 hour. Cells were washed with PBS and incubated with DAPI prior to visualising on a Zeiss Axioplan 2 upright microscope using AxioVision 4.1 imaging software.

Transgenic Mouse Generation

Transgenic mouse generation was performed by the transgenic unit at the Cancer Research UK London Research Institute.

Results

To assess the role of fbw7 during pancreatic cell-type specification, mice in which fbw7 was deleted in pdx1-positive pancreatic progenitor cells were analysed during embryonic development by generating Pdx1-Cre; fbw7^(f/f) mice. While pancreatic organ size and gross morphology appeared normal, histological analysis revealed some scattered cells in the Pdx1-Cre; fbw7^(f/f) ducts with altered morphology when compared with fbw7^(f/f) ducts (see FIG. 1 a-d). These mutant cells were characterized by enlarged cytoplasm and a nuclear shape different from surrounding ductal cells (see FIG. 1 b-1,2 and FIG. 1 d-3-4). Insulin expression, which is normally restricted to islets of Langerhans in control mice (see FIG. 1 e), was detected in mutant ductal cells (see FIG. 1 f,g). In addition to insulin-positive cells, Pdx1-Cre; fbw7^(f/f) ducts also contained, albeit more infrequently, cells expressing the β-cell marker glucagon, while insulin-, glucagon- or amylase-positive cells were never found in control fbw7^(f/f) pancreatic ducts. Quantification showed that insulin-positive cells were most abundant in the Pdx1-Cre; fbw7^(f/f) ducts (16.82% of the ducts contained insulin-positive cells compared to 2.84% and 1.89% for glucagon- and amylase-positivity, respectively. Thus, absence of fbw7 appears to trigger abnormal differentiation of a small subset of ductal cells, with a preference for insulin-positive cells.

One of the key features of functional β-cells is the expression of the glucose transporter 2 (glut2). In control animals, glut2 was co-expressed with insulin in islet β-cells (see FIG. 1 e). Double insulin/glut2 immunofluorescence (IF) demonstrated that glut2 is expressed in insulin-positive mutant ductal cells (see FIG. 1 f-g). To characterise further the induced β-cells found in Pdx1-Cre; fbw7^(f/f) mice, these cells were analysed by electron microscopy. At the ultrastructural level, control ductal cells showed no accumulation of secretory granules (see FIG. 1 h), whereas islet β-cells possessed small dense secretory granules characteristic of insulin granules (see FIG. 1 i). In mutant ducts, cells that resembled islet β-cells at the ultrastructural level were found surrounded by ductal cells (see FIG. 1 j). Thus, induced insulin-positive cells in Pdx1-Cre; fbw7^(f/f) ducts showed functional hallmarks of mature β-cells.

To analyse the induced β-cells, double immunofluorescence for markers of pancreatic cell types was performed. In fbw7^(f/f) control adult pancreas, cytokeratin-19 (ck19), a bona fide marker of ductal cells, and insulin were never co-expressed (see FIG. 2 a), but co-expression of insulin and ck19 was commonly observed in Pdx1-Cre; fbw7^(f/f) ducts (see FIG. 2 b). Triple amylase-insulin-glucagon IF revealed the existence of glucagon/insulin double positive cells in Pdx1-Cre; fbw7^(f/f) ducts but no amylase/insulin co-staining was found (see FIG. 2 c-e). It is noteworthy that glucagon/insulin double positive endocrine progenitor cells have also been described in human embryonic pancreas¹¹. Thus, deletion of fbw7 in the pancreas promotes the occurrence of cells co-expressing markers of different pancreatic cell lineages, a cellular phenotype which is not normally observed in adult pancreas.

During embryonic development, Pdx1-expressing progenitors give rise to all the pancreatic lineages¹². Thus, the emergence of insulin-positive cells found in Pdx1-Cre; fbw7^(f/f) ducts may reflect a developmental-specific defect. To test whether fbw7 deletion was sufficient to induce β-cell neogenesis in adulthood, and to clarify the cells of origin giving rise to the induced β-cells, fbw7 inactivation was combined with lineage tracing. To this end, the R26-CreERT line, which expresses a tamoxifen-inducible form of cre recombinase from the ubiquitous ROSA26 (R26) promoter, was employed in combination with R26-LSL-YFP, in which Cre-mediated recombination leads to the permanent expression of YFP (see FIG. 3 a-c). In an fbw7^(f/f) background, tamoxifen treatment will result in recombination of the floxed fbw7 alleles concomitantly with activation of YFP expression in Cre-expressing cells, and thus recombined cells will be irreversibly labeled with YFP expression driven by ubiquitous R26 promoter (see FIG. 3 b).

Adult (12 week old) R26-CreERT; R26-LSL-YFP (referred to as RY hereafter) and R26-CreERT; fbw7^(f/f); R26-LSL-YFP (referred to as RFY hereafter) mice were injected intraperitoneally with tamoxifen and histological analysis of the pancreas was performed 2 and 12 weeks post injection. CreERT mediated recombination was detected in all the pancreatic cell types, but with different efficiencies. While almost all acinar cells showed YFP positivity (97%), recombination occurred much less frequently in islet (12%) and ductal cells (5%) (see FIG. 3 c-d). Despite the high percentage of recombination in the acinar compartment (see FIG. 3 d), no insulin-positivity was observed in cells with acinar cell morphology in RFY pancreas (see FIG. 3 e-n). Likewise, direct intrapancreatic injection of 4-OH-Tamoxifen into the pancreatic tail resulted in exclusive recombination in acinar cells (see FIG. 4 a-e), with no recombination detectable in islets or in the ducts (see FIG. 4 f-g), but no β-cell neogenesis was observed (see FIG. 4 h-i). In contrast, despite the low percentage of recombination (5%), ductal cells of RFY mice gave rise to insulin/GFP double-positive cells in ducts as early as 13 days post injection, albeit very infrequently (in less 1% of ducts) (see FIG. 3 e-n). Insulin-positive cells in the ducts of RFY mice could also be observed at later time points after tamoxifen injection (12 weeks) (see FIG. 5 b-g). Therefore, fbw7 deletion in ductal cells but not in acinar cells, induces the acquisition of β-cell identity.

Fbw7 targets many proteins involved in proliferation and differentiation such as N-terminally phosphorylated c-Jun, Notch intracellular domain 1 (NICD1), phosphorylated c-Myc and phosphorylated CyclinE for proteasomal degradation³. It has been shown that SCF(Fbw7)-mediated degradation of its substrates is tissue specific^(4,13-16). Pdx1-Cre; fbw7^(f/f) pancreas showed increased p-Jun^(ser73) and NICD1 protein levels when compared to fbw7^(f/f) as detected by western blot while—Myc and CyclinE protein levels were unaffected (see FIG. 3 o). In control pancreas, c-Jun and p-c-Jun^(ser73) were mainly expressed in the ductal compartment (see FIG. 3 p-u), and c-Jun and p-c-Jun^(ser73) immunoreactivity was increased in Pdx1-Cre; fbw7f/f pancreas (see FIG. 3 s-u), suggesting that SCF (Fbw7) controls substrate abundance in pancreatic ductal cells.

Ectopic expression of combinations of transcription factors can induce changes of cellular fates (reviewed in ¹⁹), however examples of reprogramming in vivo by loss of only a single molecule are rare. pax5 inactivation converts differentiated B cell into other hematopoietic cell lineages²⁰. In the present study, it has been shown that fbw7 controls the differentiation of a subset of ductal cells into β-cells and other pancreatic lineages, implying an unexpected plasticity and heterogeneity of pancreatic ductal cells, which might be therapeutically exploitable.

The conversion of pancreatic ductal cells into β-cells observed in the RFY mice in vivo could be due to up-regulation of substrates involved in beta cell neogenesis in the adult pancreas. One of such factors is NGN3, which has been shown to be required for proper differentiation of beta-cells from pancreatic progenitors⁶. To test whether Fbw7 had any effect on NGN3 protein stability (see FIG. 6 a), a set of in vitro experiments were conducted. In accordance to the behaviour of most of the known Fbw7 substrates, NGN3 protein stability was increased when cells were treated with GSK3β inhibitor SB216763 (see FIG. 6 b). An in silico search for putative phosphorylation motifs revealed the existence of a GSK3β consensus site comprising 5183 and 5187 (see FIG. 6 c). NGN3 was able to interact in vitro with Fbw7 when both proteins were coexpressed. However, the interaction was abolished when Ser183 in NGN3 was mutated to Ala (see FIG. 6 d). Furthermore, the stability of NGN3 when mutated in the GSK3β phosphorylation site was drastically increased (see FIG. 6 e). These data lead us propose the following mechanism of action: Fbw7 expressed in a subset of pancreatic ductal progenitors would be controlling the levels of NGN3 (see FIG. 7 a-8 a). In the absence of Fbw7, NGN3 would accumulate in those cells, thus initiating the differentiation of them into β-cells (see FIG. 7 b). This model predicts that overexpression of NGN3-AA (in which the GSK3(3 phosphorylatable serines are mutated to alanine) in mouse pancreas should increase beta cell number (see FIG. 8 b).

To test this possibility, a mouse line that would allow conditional overexpression NGN3-AA in the pancreas was generated (see FIG. 9 a-d). In order to achieve overexpression of NGN3-AA specifically in the mouse pancreas, we crossed NGN3-AA mice with Pdx-1-Cre mice (see FIG. 10 a). NGN3-AA; Pdx1-Cre mice showed good recombination of the R26-LSL-NGN3-AA allele in the pancreatic tissue, while liver or tail remained completely unrecombined (see FIG. 10 b).

Accordingly, NGN3-AA protein was detected in the pancreas but not in the liver (see FIG. 11) concomitant with an increase in the amount of NGN3 and Ins1 mRNA (see FIG. 12). Furthermore, the number of insulin islets analysed by immunohistochemistry performed in NGN3-AA; Pdx1-Cre pancreas was increased when compared to control littermates (see FIG. 13). Thus, over-expressing NGN3-AA in mouse pancreas is sufficient to cause an increase in β-cells. Therefore, NGN3 is a likely substrate through which Fbw7 exerts its function. These data suggest that, inhibition of NGN3 degradation by Fbw7, for example by inhibition of GSK3-β, may cause the de-novo generation of β-cells.

To address the differentiation potential of pancreatic spheres in RFY and RY mice injected with tamoxifen, an in vitro culture system that enriches for ductal cells was optimized. Cells isolated and cultured as described in the Methods section were monitored over time. As early as day 4 post-plating, pancreatic spheres were observed in the cultures and remained constant until 15 days post-plating (see FIG. 14). Both RFY and RY spheres showed GFP positivity under the fluorescence microscope, suggesting they were originated from recombined pancreatic cells (see FIG. 15). Furthermore, when day 4 spheres were dissociated and replated in attachment culture conditions, some RFY spheres spontaneously differentiated into adherent cells after day 8 and the effect was even more drastic after 14 days post-replating (see FIG. 16). Interestingly, Insulin and Ck19 staining revealed that both cell types coexisted in the differentiated structures (see FIG. 17). These data suggest that Fbw7 deletion spontaneously induces the differentiation of pancreatic spheres into β-cells in vitro.

To explore further the functionality of the newly formed β-cells upon fbw7 deletion, mRNA expression profiling of GFP+ sorted cells from tamoxifen injected CY and CFY mice was carried out and the results were compared to those from GFP+ cells from MIP-GFP mice as a positive control for β-cells (FIG. 18A). CFY GFP+ ductal cells showed increased expression of β-cell specific genes iapp, ins2, nkx6-1, chga, pdx1, pyy, nkx2-2, insm1, pax6, pcsk1, vegfa, gck and slc2a2 (FIG. 18-A). Q-PCR analysis confirmed increased mRNA expression of β-cell marker genes (ins2, gck, pdx1 and nkx6.1) in CFY ductal cells when compared to CY GFP+ cells (FIG. 18-B). Furthermore, insulin+ cells in the ducts of CFY mice co-stained with the functional β-cell markers c-PPT, Glut2, MafA1, Nkx6.1, Pax6, PC1/3 and Pdx1 (FIG. 18-C) while they were negative for the ductal markers Sox9 and DBA (FIG. 18-C).

An important hallmark of β-cell function is the ability to release insulin upon glucose stimulation. To test this in induced β-cells, GFP+ cells from CY and CFY mouse pancreas were sorted and challenged with glucose treatment in vitro (FIG. 18-D). While GFP+ cells from CY pancreas did not respond to glucose, GFP+ cells from CFY mice showed a substantial release of insulin (FIG. 18-D). Thus the induced β-cells show both the characteristic marker expression and functionality of mature β-cells.

A glucose tolerance test was performed in R26-LSL-NGN3-AA and R26-LSL-NGN3-AA; Pdx1-Cre mice. Mice were fasted for 12 h before glucose injection. Glucose levels in the blood were measured at the indicated times. The data suggest that NGN3-AA transgenic mice show improved glucose tolerance compared to control mice (FIG. 19).

TABLE 1 Fbw7 protein sequence Fbw7 protein Human (Homo MNQELLSVGSKRRRTGGSLRGNPSSSQVDEEQMNRVVEEE sequence sapiens) QQQQLRQQEEEHTARNGEVVGVEPRPGGQNDSQQGQLEEN NCBI Reference NNRFISVDEDSSGNQEEQEEDEEHAGEQDEEDEEEEEMDQ Sequence: ESDDFDQSDDSSREDEHTHTNSVTNSSSIVDLPVHQLSSP NP_361014.1 FYTKTTKMKRKLDHGSEVRSFSLGKKPCKVSEYTSTTGLV PCSATPTTFGDLRAANGQGQQRRRITSVQPPTGLQEWLKM FQSWSGPEKLLALDELIDSCEPTQVKHMMQVIEPQFQRDF ISLLPKELALYVLSFLEPKDLLQAAQTCRYWRILAEDNLL WREKCKEEGIDEPLHIKRRKVIKPGFIHSPWKSAYIRQHR IDTNWRRGELKSPKVLKGHDDHVITCLQFCGNRIVSGSDD NTLKVWSAVTGKCLRTLVGHTGGVWSSQMRDNIIISGSTD RTLKVWNAETGECIHTLYGHTSTVRCMHLHEKRVVSGSRD ATLRVWDIETGQCLHVLMGHVAAVRCVQYDGRRVVSGAYD FMVKVWDPETETCLHTLQGHTNRVYSLQFDGIHVVSGSLD TSIRVWDVETGNCIHTLTGHQSLTSGMELKDNILVSGNAD STVKIWDIKTGQCLQTLQGPNKHQSAVTCLQFNKNFVITS SDDGTVKLWDLKTGEFIRNLVTLESGGSGGVVWRIRASNT KLVCAVGSRNGTEETKLLVLDFDVDMK (SEQ ID NO: 1) Mouse (Mus MNQELLSVGSKRRRTGGSLRGNASSSQVDEGQMNRVVEED musculus) PQQQARHQEEEHTARNGELVGANPRPGDQNDTQQGQVEEN NCBI Reference NNRFISVDEDSSGNQEEQEEDEEHAGEQEEEEEEEEEEEE Sequence: MDQESDDFDPSDDSSREDEHTHNSNVTNCSSVSDLPAHQL NP_001171245.1 SSPFYTKTTKMKRKLDHGSEVRSFSLGKKPCKVSDYTSTT GLVPCSATPTTFGDLRAANGQGQQRRRITSVQPPTGLQEW LKMFQSWSGPEKLLALDELIDSCEPTQVKHMMQVIEPQFQ RDFISLLPKELALYVLSFLEPKDLLQAAQTCRYWRILAED NLLWREKCKEEGIDEPLHIKRRKIIKPGFIHSPWKSAYIR QHRIDTNWRRGELKSPKVLKGHDDHVITCLQFCGNRIVSG SDDNTLKVWSAVTGKCLRTLVGHTGGVWSSQMRDNIIISG STDRTLKVWNAETGECIHTLYGHTSTVRCMHLHEKRVVSG SRDATLRVWDIETGQCLHVLMGHVAAVRCVQYDGRRVVSG AYDFMVKVWDPETETCLHTLQGHTNRVYSLQFDGIHVVSG SLDTSIRVWDVETGNCIHTLTGHQSLTSGMELKDNILVSG NADSTVKIWDIKTGQCLQTLQGPSKHQSAVTCLQFNKNFV ITSSDDGTVKLWDLKTGEFIRNLVTLESGGSGGVVWRIRA SNTKLVCAVGSRNGTEETKLLVLDFDVDMK (SEQ ID NO: 2)

TABLE 2a Human Fbw7 nucleotide sequence Human (homo sapiens) Fbw7 nucleotide sequence NCBI Reference Sequence: NC_000004.11 (SEQ ID NO: 3) ccttccgcagctgccgcttcagtccgaaggaggaagggaaccaacccactttctcggcgccgcggctcttttcta aaagtgtgagtggccccgggagagggaattgagggggagaaagtgggaggatgccggcgcttcctcctggagttt tgctgatgttctccagtagtgttgggagtctatcaatgagggagtctagctttattcacggaggggggaaggtgt tagggaggcctgtattttgtaactttttagctctttgtacgagtaatgcaagtatgcaggtttctgtcggaggag tcttttctccaaagtgaatactcgcacctttctggccagcagttggcaaagggaaattgaggaagccagagcata tgtgcgcacagaggccatctgtatctgtaaccttttcctggtactgtgggtgtgtgcggtgtactttttgtttcg ggggtactctcatataggttcccgaatgagaactacttaacctgtggacccctgaaagtaaaattccttttttaa aaaacaacaacaaacctgttttgagagaggttcatttcagtacctattggtacaggagttaaccttctcagttac tgggtccaatttcatgtgtgtcaggaggacactgtaacagatgtcagttactcaggaacttgagttgttaggaat cacaacttacttggggaagagcatttatcacattaatcattttataaaagttgtgattttttccctttcaccccc ccctttttttttttagagtaacaaattacagcacttttttatttggtttgaggctttgcttttttgaattatctt tatttctgtatcaatttaggatacatctttgatatttaagtcaggaatgattttttccttccattattttaaagc tgaagtttataaaacctttgacttgtattttaggtatcttgtcagagtaccaaattaaaggtttatgaaagtaat taatatttcatatacagaccttaagggttaaacatagagcagaattttatgtaagaagacatagacagtagtata ctgagaattgtggtccgtctagacaactgtgggctcgttctgtctgaagccactgctgagagatactttgtggga atgaatgtaagcctggggttatctctcttttcgaagttctccattcaggatcttgattagattaatttaaaactt tctcaaacttaacaatgtttggtccttttttttaaagtaatgagttctgcaagtttattagcctgtgtattatgt gacagttcatttacatattccatacctgtgtccctaaactttcaggacacagtcagagtttgtgtttcaagaatt atcaggacaagcctcagttcatcttttcttaattttatggactttcattatttctaccatcagtttctccaactg ttgtcttagaataatgtctttcatttgaccacttttccctcctctggaacttctcccactgaattgtttttcttt agttgccttgttatgctttggtaaggaactttaaattctgtcctttaaatgtgtactgacatgaaaatactgaat agtggatgtaatgttagaatcagagtcacgtattctgtttttcactaagatattgcaaagagaaatgtagtcatt ttctaaaatactgtacccaaaatacagtagaagctgtattttgggaaggaggggaggacaaactaagaaacaaac tatagtttgaatgtaggttgcactctgtcaaagttgcttactaactttaggacctacttttattgcttatggatt ggttaaaaattacggtattttttttttttttgagacagagtcttgctctgtcacccaggctggagtgcaatggag cgatcttggctcactgcaacctccgcctcccaggttcaagcgattctcatgcctcagcctcccaaatagctggga ctacaggcatgtgctaccacgcctggctaattttttttgtatttttagtagagatggggtttcaccatgtgggtc aggctggtctagaactcctgacctcaaatgatctgcccacctcggcctcccaaagtgctgggattacaggcgttg agacaccgcgtcctgccaaaaaattatggtaatgttttattgcacgtttgggtagaaatgacaaagtttttgggg tccctgcctttgagatgcttttacttgtaggtggtggagacaatcaaacacaaattgcacaaagctataggagag gggggatagaccaatatttatctaagaccgcactggatgttagatattgtcaacttatagatgtgaatatatttt tacatttttcactagggaaatgttgcttttggacgtgtacatatagaactaattgaagtattgaagccaaatctg ggaaggtttcttttaggaagaaaagagcaagtggagacacaatatttaagacttttggaattgaaaggcataagg gacagataaaattacgaagctggtttgaagggaatacagatagaggttgttgagtagtgttagggtcagttgtgt ttgtgtagacacatgattacagttcaaagaagcaactgagaagagtgttcctgagagtaatttcagatgactggg gagaaattacattttaggtaaaagctgtatgcctggattaggattttgtttttctagggaaccaacatttttatt atatcgtggctatccagggctatacagaaggaggagtgaaaaagaagatgcttagtgtgttgaaagtggcttctt acaaaaaagagtttagaaatgggacagtagactttgctttggatgtgaaattttagggcaacaattggatactgg tgcagtttcaaaacagcaaaatagtttagcaggtaatatactgaaaatgtaaagttggaattattttcaaagtat ttaggtttcccagagtactgatggaaagtgaagagctgaaaagtgggtatttttagttagggggaaaatctgaat gaatgagacactacctaaaatagatgttttagaagtggataagagttgaagttaaaaaggaatgagcaggcttta aacatggccaccagtttagtcccagggtatattattctgtatttttgaaacaagctaaaattttttttttttttt aaatagggtatataggtcccttgagattaagttcagaagagtatacttaataataatttcattgttaaggaattt ataaatagaaggacagatttgtttcctcaaattcatgttttaaagttcactgtgcattattatcctacgtttggt cttgatatgacgaagtgaggccaaaaatagctgacagagaaggctgcttcccttgttcacagttggtgtgtgtct ttgtgtcccatacaatggaggggatatccctgtgtaggcacagtagtgctgaacatgctttttttttttttttcc ttttaaactttttattaaaaaaaagcaacttggcatttaaaaaaatgtagacatttttgcggcttgcatttagta ttgactttaacaggattaaggattgtgaggaaggaaatatatattcacacttttttgtgttccttagttggaatt ctgcaccttcctagtagcttctctgacttcttaacagtgattattcaaatggcagaaactctggggaagagatgt ttcaacaaggagataggtctaaggtgattctagggtgaaaatatagggattggattatatgccatatggaggcct tctcttgtcatcctccactttccacaacattttttgtgcagtcgaagccatgttcagatgaattttagaaggcag ccacttatagttaatgttatttactagtgaggacagtgggaagagtggtaacttcatgtactttgccttggcaac ttttggattgatagggaattagagagccttgagggtatatgtttgtgtgtgtgtgtgtgttttagcacccaccag atgaccccaaaggccatgcataggctcatttcattgtactttggagatggttcttggagaagtcttctggtgctt ctgacatctcattgtgtagagaaagagttggcaaactatttttgtaaagggccagttagtaaatattttaggttt tacaggccacatgatctgtcacaactttcaattctgttcttatagtgtgaaagcagctgtagacaacaaggaagt gaatgaatgttgccgtgctccaattggcccataggccgaagtttttggactcctggtatagacaactgccaaatc caccaacaaacagtatgagctttattacactacaaagccaggactggctatttttggtggtctttgttttctaga tgagatgtttttctctgagttcatttaaaatgattctgcataaagcacatactctactgtacaaaatacttttgc cttcatggtgatgaaaactatggattgagggtcggggtgataccatctttaaagggatgcagctaaaaagtaggg actacctgcatttcttatctaaagataaccacagttggcatttagtgatatactggctttgtatcatggccaaaa gtatctgtaaagaagcttgtgggcatacttttgaaaccttctgaaaataaatgtaaagcatttacttcgtgtgta aaattagaagtgtctatggttggtttgaataatatgagtcaaattataagagcattaaagtttgatactgctatc tattaaagtcttaatctcttgcagtggctaattcagtgtgtagaattacacatcgcaatttttgttttgttaatt tcaagtactagtgtatattaacaaatcacttaaatcatttaaatgtgtactttaaaaagctttgatgtatataca tatatatacgtatatatatatatgtacacgtatatatatacacgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgt attttttttttttttgtgacggggtctcattctgtcacccaggctggagtgctgtggtgtgatctcagctcactg caacctccacctcctgggctcaagtcatcctcctacctcagcctctcgagtggctgggatcacaggcatgcgcca ccatgcccgactaatttttttgtatttttgatagagatgggtaacaacttttggattgatagggaattagagagc ctcgagggtatatgtgtgtgtgttttagcacccaccaggtaaccccaaaggccatgcatcatgttgcccaggctg gtctcttaactcctgagctcaggtaatccatctacctcgacctccctcagtgctgggattgcaggcgtgagccac cacacctggccagcattgctatatttttaagagtgaatctttctcacatgtgttgttcagaaagtgtaattcact ttgtgcaattttacattacagttaatattaaaatatttgtttcatatgtcagcagataccaacattggatgatgg tatatttagtttcataaatcctatttattttaggattcccttgtttatggcaggggtggagattactcttgcaat aatgatcactttttagttagtttgaaacctgcttttagtttatatgtagtatttctacatattgttacttaaatt gaactaacaatttactttttaatttttcttcttccttttaaaaaaagagtatataatctgacaatgttagtacat cttttagaaggtcactataaagttgctttccctatttatttggaaaggattatttccttattattttaaaaaatc ttttaatttttgtcttttcaagtaattttatcattgtcccaagcctaaggatgagtggcaatttaaaagacacaa ggtgtgcatcttctatctgcaaatactccaaacagaaattattccagtttgttgatactttgagtggaccaggga aaaatgtgtatgtttttagtgtaactgaattgtcattacaaaaagatgagtaaagttgctgttaaataatgaatt cttgctgttaatttgtcctgttcttagactttcccccattaagtataatgggggaaaatgtacacacacacacac acacacaaagtatgtatgtgtagatatacctagcgtattgtgctagatgttcatttctgcatgactgctctggaa tttaggcaatattaaaagaccttacaaaatatttttaatatcacttaatatcctgttttatgtcttaatgtactt ttatgtttttgctgctttctgatgatacctccttggtacttcctcatgatacctttcacagtatgtttaaatgga atgtattttctcaaagtacgtgacattttctgaacactatgggatatatatcttctagtaattatatttttacat gcaagtatctttaaaaatttcttcctattacaacttaagaattgtgaaacatttgagggagcatctgcagtttga gcacttgatctggatattggaatgacaaacttaacatttaactaaatttttctgaggtgttcttttacaattttt gagttaacacttgatttctgtaaagcagaaggcttataagaagaaaatatgagagccagtgttcgtcttgtatta tcctgccatgcacgtagttgaaaatcagaaacaaacagaaatgcacaagagaccttattgcttgagtatcttctg ctcttcccccatcagacctaagggacagctagtggatcagtcagatactgtgcagtttctgttgctatgctgtgt cacctttgccctttctttctatctggaagatagctactggaaatctggctgtaagaaagagatagcatgtttttt tcctctctctctctgtgtgtttctgtctgtctgtgtacctgtacctagtgtattgtgttagatgttcatttctgc atgactgctctggaatttaggaaatattaaaagactacaaaatatttttaatattatttagccttttctctgaga ggatcacatcctctggctttggcgttttcatctcacagcatcttaaacgaacccgtcaatgtttattatcagttc caaatgtgaagtctcaaggcaaattgttggtttgtttttggaagaatgtatgtgtgtgtgtatatatatgtgtgt gtgtgtgtgtgtgtgtgtgtgtgtgtgtgtatataatttttaaatttttaacaaaaattcctgatctagtcaatg cttatgagagtgtgtgtggtttgtataaaattattttttttagagacagggttgcttgttgtcaccaggctggag ggcagtggtgtgatccttgctcactgcagccgtgaccccctgggctcaagtgatcttcccaccttagcctcctga gtagctgagtagctgggactataggtgtgtactgccacaccctgcaaatttaaaaaaaaaattctgtaaagacag ggtctcactatattgcccaggctggttctcaaactccaggactcaagcagtcctcctgaaatggcctcccaaagt gttgagattacaggctggggccacagtgcccggtcagagtgtgtgtattttttaacagccttttaggattgcatg ctttttaggggggtgaatgaggggtaatatacctttgcatgacattctctaggtctgagaaggagttgcttcact aaaagtgtaatttataattaccaaatggttgaaaacaggtctacctggagtcagcaacaggcagctagtttactt ctcatttacgtaataaaatacattcataatatgcattcagtaattcataattcaataatttcttggcttcatggc cttggacaagctatttattatccttttaattttggtggcatcacctgttgatggaggaaaacgtgtcctttacat ggtggttgttaggattaaatgagataatataaagcatgcaaagctcttaatccagtgcctggcatatagcatttg acccatcagtgttaatttattaagttaaagttgttttaaagtgtgttgtagatagaagatttaaatcaggcctta tattaagcccaacatttaaattcaacacttgagtttgacaaataatactgaagtaattctgtgttttcagaaaca tctaaaacttcattaacattttattaaaaactgaagaaatgacagaactgttgggcttttttttaatagaaaaga aagactgatttgaacataactggaatttgaatttagtttcaaagatctcagtagtgcatggaccagaaaagaatc cgtttttgaatagttagtgattatgatagtgactgtggtttaaattgcccatttagaaatagcaggatctaattc agttatcagcctttttagttgcccatctctttaacccctggaaataaaaagctttaaaaaatcattaagaagttg aacggaaattttatctgtgtcatttacatctcaaattaagatcgaaataattttgtttacatgattctattttaa ctacttaattttagtttttgttttttcctgaaattattagtatgtgggttttaaaaattcctttttttgtgtgct taatatttgttaaatacaagattggttttcagaaataccttttcttcgagtagggcataatcagtgttttattgg ccaatacaataaataaatgcttcttgattgtttttgtatttggaaagaaattctatgttgagaacagttttggta tgctttccttcattggctgtttattgaatagaactgtatatttaaggtatatgaaaaaaatctaagctagaaaaa aagactgagatacattcttggaagaaaatacttgcaaactatgcatctgacaaaggtctattgtccaacgtctat aagaacttaaatttacaagagaaaaacgaccccattaaaaagtggacaaaggacgtgaatagacacttctcagaa gaagacttacatgtggccaacaagcaaagaaagctcaatattactcattagagaaacgcaaatcaaaaccacaat gagataccatctcagactattcagagtggctactataaaaagtcaaaaaataacagatgctagtgaagttgtgga gaaaagggaacacttatatactgttggtgggagtgtaaattagttcaaccattgtggaaatcagtatcgcgattc cttaaagagctaacagcagaactaccattccacccagcaatcccattactaatgggtatatacccagaagaatat aattcattctaccataaagacacatgcacacgcgtgttccttacagcactaatcacaatagcaaagatgtggaat caatctaaatgcccatcagtgacagattggataaagaaaatgtggtacatacacaccatgggatactgtgcagcc ataaaaaagaaggagatcatgtcttttgtgggagtatggatgaagctggaggctattatccgtagcacactaatg caggaacagaaaaccaagtaccgcgtgttctcacttataagtgggagctaaatggtaagaacttatgaacaaaat gaatagacactggggtctacttgagggggagggttggaggagggagaagagcagaaaagataactattgggtact gggtttaatacctggttgttgaaataataagtacagcacaccctcgtgacatgtgtttacctatgaaacctttac atgtaccccgaaacctaaaataaaagtttaaaaaatatattctttagcactgattatcttgctagctgtgacttc tccttttaaggaaagtttgctttagattttggtgtaaatgtagaaattagatgggtttatctgctagtggctctc tagttagatgattaatgaatattctagtttaaaaaatttcagcattttaaatacttttctttattggtaacgatt tggattatgcttctagggatctttacatttcatagtttttcaaaaaagactttataatgagttcctggatgggaa gaagagcatgttctctctagtttatttgatttcttcctgattattatgtatgtaatactggaaggttgtgtaaat aaatatttgggttgtaagttttcttttgcatgattggtggtctcttaattgactgctgtgtgaatttgatagtat aaactagaatgcttatacagtgagtggggaagcctttttctactgagtgttatgcaggtggatttttcccccctg cggataaatactaaacttttatattaccagatttagacatttagacaggaaacaaaatttccttttttttaaaat aaatgagcgggagaagagagtggggttgtacagaggtggggagagaggcagtaaaggagggaggaaagaagaggc agatatcagggagagtggctccggaagttttgagttcagtcttagtcagaggtcacaccctgttatttctttggg ttgtggacatgaggtgttaccacttgttatctgtcaccctttgaaatgatgattagattattggtttttattcat agcttttcaacatttgggttctttaaaccattgaaggtttagtttgtatgggataatcagtgttattttagggtg ggaaaatttccaccatacctgctaggagaaggggatggccaaagagtttttgacttacagaggagtttttttgtt ctgttgttttttcctcattgactctatttcgctagctttgtctgagaggggtgcttataattctatatgtgacat agtagctgagaggcaagctttggagttgatagacccaggttccagctctgctgctttgtgatttcaggcaagtta tttattccatgtgtgtttcttttaaaactgggatgataataatagtacctgtttcccttatagagtaattaagag gattgagtgagataatgcatgtaagcctttagcacagtgtctgcctaagttagcatccagtaaatgttagctgct gcaataataagaaatattatagtattaagttattgttatttctgtgagggtgctggttttctgaatgctgagaca ttggtgatctttgctcctgaacgtgttactatttggaatactgcctttattcagcagcagattcagttcagattt actgaacacctactatgccaggaattgatctaaataagcaaaacaaaaatcctgctctcttgaaacttactttct ggttaggagagacaaaatacacaaaacaaaatagatgatgtgtcactcaggaaatttggttgttggactttgtct aagctttcattaaccaaccctagcagtcttaacctttacatactttttgattgttcctgttgctgtgcttgagag tgtttggaggtaggggcaccctaaaagcgtatcatagactagctttattgtctccagcttcagcttgatcctaat tttaaggcagccttaaatgatagttttcatcatttccttgacttactacttaaattggctatctcaaaactttca gtccccaaaacttgtatttcacatcattcagagagatgttgctcctccccagctgctcctcttttgctcttctat gccattttcttaagaggaggggatactagaagcatctttttagatttattttctgactagtctaactgtatactt ttctttctcatattttgtgttttctaccctttatttattgttaactgatacatttaatttgcttattttctttaa aatatgtgttttttttggtctctccaattaaattgtgaaagtttttaggtcagggaccacttttctattttgacc ttgagcttactctcttgaaaaaaagataataccacccagggatgttgtgagacctcaaagagataatgtgtgaaa ggggtcctggagccagatccttaataaatgttagtttcttctttgaaccactctagttgctgaaactgctgtgca tataatcagtgcttaaaaaatgcttgttaatttatcttgaattgagtttgtgaactttcaattgtcacttggtgg tagttttagtttttagtgctagataagattcaaaatactacttttcaagtgtcagatgataaatgctgggtcatt agtggtagagctcaacagtgattaggagcaggaacttgagccatcattgtttgggttcaagtacaggctaccaca cttactggttgtatgacctttggcaagttgtttaacctccctcggtttctttatctgtaaggaggggaaaatgat atctacccacctcatggttacttctttgttagtattaaacgaagtaaatgagaagctcgaaacagtgcttagtat ataacaatctataacagggtccccaggggacatttggcactatttggtgatgtttctgatggtcatttgggggag tggggagagggaagggtgctactggtatctcgtgggtagaggttaggtataaacaaggtataggacagcatccac aacaaaaaaattacctggtccagaatgccagccctgccaagcaacattgggaaacccagcttagttcctttgtat aaggtactttgccagttagattctgtatacatgctgattattgataaagatgagtcacagacatggataggcttc atttaacattcattttaaaagacagacatgtaagaaggttagtaggtttcatttctttttaaggttacagggaaa gtactccaaaattaatctaaaagtccttacacttttttcagaaactgagaaaaggttccagatgaattttactaa tttaatttttttatgacgagctattcatgtaaaagtgtattatgaatagagggcttatgagatatgagtgccgta gtgtgtattcataaagtcaaacgtacacttaaaaaacaagtatcaaggtaaatggaatgatctattgggggaaac cttggagctctctctctttccatatgttttattactcaccaagtcaagggctctttaacttaggatcttgttttt ctgtgtacacttttcctgtttactgtttaaaattggacccttactaaaaatacagaaaattagctgggcatggtg gcgtgcacctgtaatttcagctactggggaggctgaggtagggagaatcgcttgaaccagggagacggaggttgc agtgagctgagatcgcgccattgcactccagcctgggtgacagggcgagactcagtctcaaaaaaaaaaaaaaaa aaaaaaaaaaattggacccttaccattccttgtccatttcaggagcctcttaattgccttgcttctgttctttct ctgtattcccacttgaattgactcttctttgctgggatccctttgggatcccttctagtccagatccaatctacc cttcagagctccttccaccgtccccttatttctgggagagaattcagccttaataccctaaggatggaatttgaa tgtactgacttttctgctatttacagggttgtgtattatctttggaagaattgggaagtcatatcattttctgta tgttgtggttcaggtgacttgtttcacttgtatccaaacactggttgtgtgtccatgtatatacagcatgtatgt actttaatgtctatttgtatgtgtgtgtacatgtatattctttcatgtatatttgtctctacaccttgctgtttc ctctgcttggaatattcttttcttttcatctgggaaacatttgtctcttcagaccctacttaaatattgatctcc taggaagtgtgtcctgattaatataactagaattagttatttttttccactggttacccacagtatctctgtggc ttttatagcacttaacatccttatttgtatttttcttttctactgatctctcagttctttgagggcaagagtctt gtcttagtcaacacctgctcccccaagtacctaacattcctgccacattgtagacatatagtatgttaacaaatg aatgggagagacaaaaaactggaaagcttttaaataagttctatgggggttggcaggaggggagaccacacatta tcttgtcacctttgtttccaagtgcttagtgttgtgcttgcccataggtgttcagcaaatatttgttgaatgaat tagaagcaaatcccaggagctgataaaattttcatgtgaacttttttcttctgcatttctagaaagcatatccta gaagtaaagtagcttagatattttggaacatcttattcttaaagtcgaccttcctatttttgactcagtagtgaa actttgatccaatggatacttcctgaatagtgcttttggctgattcctttttcacaacataggtccgttctggtg ttgagtttgttggaaagatgaatatgtagtatcatttttacatcatggaggagccatcattaagtaacagtgtta aataaggactggtcccctttgcacatttgggcccaaattaagtgagtatgtactcttaagtatgggtaatgattg aggctattgaggtgtaggaaagaaagaggacaactggaggtgaagaagtgacctactagatctcttttgcttttg gtaatgattgattttttttttttttttttttgagacggagtctcgctctgtcgcccaggccggactgcggactgc agtggcgcaatctcggctcactgcaagctccgcttcccgggttcacgccattctcctgcctcagcctccccagta gctgggactacaggcgcccgccaccgcgcccggctaattttttgtatttttagtagagacggggtttcaccttgt tagccaggatggtctcgatctcctgacctcatgatccacccgcctcggcctcccaaagtgctgggattacaggcg tgagccaccgcgcccggccggtaatgattgattttttacccccagttatttttaaagaattggcaaggaaggaga ggggtatgaatctagaagataagaatgtagattttgtaattcatgatcattattattaccatgtattgaatgttt actgtatgtcaggaactgtcctaaggatttttacaaccgttatttaacttagtcctcacagcagttcatatgaaa tagctactgatgttttatggcaaaggaaacaaattcagagaaatttccaagaacacatcggaattttgacttgca gaactgggattcaaatctagatctgtcatactccatggtctatgcttttaatcatgcatgctgtctcttaagttt ttattgaaaatggaaagttgtactgacataggaggggaaaatgatagaaaatcatcctattggaaggcagattaa cagactcgtgaagaagcaattcccagttttatgtcattatacaaaataattgtgaaattgaagtccgtcatttga attgatttgcattaaccctcattttttagtcttgctcttggaatcatcatcaggacaggtgtcccagatatgtta gcagtagaataaatttatctaggcaaaatctgtctaagattatggagaaaactgattttatatgtgaaatgtgtt gttgagtgagagttaaatcttttgaaggagattatttcaggatgagcctcaaatacattatttaggatttgaaaa cctatggtttagcagaaaatctgagaatcaacatttgttagtctcttgggtataaattgcttaagtcagtgggta tctgattattctttctttctttctttatttatttatttatttatttttgagacagagtctcactctgtagcccag gctggagtgcagtggtgtgatcttggctcactgcaagctctgcctcccggattcacgccattctcctccctcagc ctcctgagtagctgggactacaggcgcccaccgctacgccctgctaattttttgtatttttagtagagacggggt ttcaccgtgttagccaggatggtctcgatctcctgacctcgtgatccacccaccttcacctctcaaaatgctggg attacaggcatgagccaccacgcctggcttcccttatgtaatttcttaaactatttttgtttgctgatttggtgt ttgtaatttaaaaaaaatttattttttcttcttttacatactttgtagaactattccctacccagtgagtttatt tatagatatttcatatttaattaatactgtgagaacatagtttttatatctgtatttttgttttttcattaggat atttaaaataatttttgtaattctgttaccttaaaaagagctaaacttagatgttggcctttaaaaatgttttgt gaaagattttctcttggttccatttaaaatactgtatttgtaactatagttaaatacatttgaactttgcccttt tcagaaattttaattatatgcctttataaaattttatcaaaagttacatgcaaatactaagtatagagtgctgaa gctgagcgtgatggcttatgcctataatcccagttactcgagaggctgaggcaaaaggattgctttaggccggga gttggagaccagcctgggcatcatggcaaaaccttgtctctacaaaaataaaataaattagctgggtgtggtggt gtgcacctgtagtcctagttacttgggaggctgaggtgggaggatggttttgagcgtagtagttcaagactgtag tgagccaagatcctgccactatactttagcctgggtgagagagcaagaccctgtttcaaatatataaataaataa aatagtaggctgggtgcagtggctcatggctgtaatcccagcacattgggagtccaaggcgggtggatcacttga ggccaggagttccagaccagcctggctggcatggtgaaaccctgtctctactaaaaatagaaaaattagctgggt gtggtggtgcgtgcctgtaatcccagctactctggaggctgaggcaggagaattgcttgaacccgggtggtggag gttgcattgagctgagattgcaccattgcactccagcctgggcgacacagcgagactttgtcttgaagaaaaaaa gaaaaaaataagtattgagtgctgaatgatgttctataaaagattaaaaatgcctaagtggaactaaattctggt aaatagaatttaggggttggggtagaaagaatcagataagtacttcaagattttcagactttaagtctaaggctc attagtatcctctgacataatctactacaggtttcctttcttagaagtgaatgttaatatggcaccactatacaa ataatatcgatatcggtatttgagtgtttattatgtgacaggcactgttctgtgtccttcatgtgtattacctca tccagtcctcacgatagctctatgaggtaagtgttgttaatatccctattttatagctgaaaatttggcatagag aagttaaacatggtcaaggtcccacctttagttaatagtggagctgggattgtttccaggcagtctggctctaga gcctgggaccttaatctttattctatactgtctgtgtcatctgtaaatttatttcttatatattatttatagaac agtgcctggcacacaataagagccatagaagtactggctattttcattggcatttaattatatatgaggttggca gagcatgcattattattccatttgtttatttatactattaattagatagtagactttattcagatttctgtgttt cctttttttaaatagtcgagtttaccgaagttcttagaaactggatgattgcctccaataatgtggccatgctag atctcaaaattaacttttttaaaaaaaccacccctccaactttatttacacttccttagaaatggataaagacca agattgggcttctgttttgatatgtcattggtttagaaaggcagaaacagcctggcacggtagctcatgcgtata atcctagcactttgggaggctgaggcgagtggatcgcttgagctcaggagttcgagaccagctggacaacatggc gaaaccatctctactgaacatacaaaaattagctgggtgtagtggcacttgcctgtagtcccagctacttgggag gctgaggcaggaggattgcttgaacccaggaggtcgaggctgcagtgagccaagatggtgctactgcagtccagc ctgggtgacaaaataagaccctgtctcaaaaacaaacaaaaaaagcggaaacaaccatactaagtttacagtatt gtttgtgtgcctacttggtggaagaaataaattcttagaagttacactttctttaaaaaattaaaaattaaaaac tgtgcagtgttgagattagtattaccgcttatcttgctgacttgtgaaattgaattacatcacttataataatgt gaagttttagaaattgttttgatgataaagagtagcagcctttaagagaaataatataaaccatgtttcctcaaa tctaaatggtcatctgtatggtattagttttccatcgctctacagaagcagtccccagcctttttggcaccaggg accatttttgtggaagacagtttttccgtggatgcggggaaggagaggcagtggggaatggtttgggatgaatca tcaggcattaggttctcttaaggagcacctaacctagatgccttgcatgcacagttcacaatagtgtttgtgctc ctatgagactctaatgccacagctgatctgacaggaggcggagctcaggcgataatactccacctgcagctcacc tcctgctgtgcggccgggtttctaacaggccacggaccagtaccagttgtggacccctgctctacagcaaattaa cacaaatttagcagcttatcttacagtttctattggtcaggaatttgggtatggtttagctgggtctttcctcag gttctcactaggctgaaattaaggaatcagccggggctcctatctcttctgaggctctgggttctcctccaggct caccggttgtgggaagagttcaattccttgttcttgtagatctgatgtccctgtttgcttgctggctgtcagcca gggaccactttgagctccttagaggcccacccccgccaccatgttcttgccatgtggccaagttattatgttgtg tatgactaaggaagaaaaatgctggattaaaatataagacaaattcttactttatgtatgactaaggaagaaaaa atgttgaattaaaacatgatacaacacattattgcttggacagtgccttgcaaaaaaaaaaaaaaaagagagttg tggttattcttccagccacaatatctgcttcagaaatgtcaaatttattttctactgttgtgtttgtgttctttt ttgtaaacaacttttttgtttcaatgctgaatcatagtattttacaggacatttaaaattaagaagtaagcgtga cttcttattgtactactaaatgtacagaatttagtaataattatggtgatataaactgccattacccaatttgca agtgtacagggaatgacagctatgttgtgactgccatgggcaaatggtgatttgtaaagcattgttgcttttttc cccccattttaagttgattttattgagatataatttatatacagtaaactacaagacatttctgtcaccctcaaa agattcctcatgccccttcacagttaaatccttccctgcaccctcgcttcaggcaatcaatgatctgctttttta ttactacaaattagtttgcattttttagtattttaaacagtgaggaaaaaagaggagatggagacaggctatttt taatttttatattttcttctttgttcttagagaaatcctagtttctttattaggcattaggtttattccttcagt gtgggggtaaagtgattcttcttgaagctttctaagtatgtttcctagatgttcttgacctctgttttgcctaag ataacttttctccagcatacccagagttggctctcttaaaccatacgtgcttgtgtagctccctcatccatattt ctacttattgtcttttcaggaccgtaaacagaggtatttttgacttgacattctcagctcctaaataatcttata gtgttactgaggcttttcacctgtcacatacccccgtcctagtctctttttgtgttgctgtaaatacccgaggct gggtaatttatgaagagaagaggtttatttggttcatggttcctgcaggttgtataagaagcatgatgtcagaac atctgctttctggtgagggcttcaggaagcttccactcatggcagagagtgaaggggagcaggtatcacatggtg aggcaggaaaggagagaaagaggagagggggtgccaagctctttttatcagttctcatgagaacttacagagggg aaactcattcattaccttgaggatagcacaagctgtttatgtggaatctgctcctatgacccaggcacctcccac tgtgccccaccctcaacattggggatcagatttcaacctgagatttggaggtgtcaaatattcaaattatatcat cccctagagaacatacctccccacccctgagaatctcagaatttcttgttgagaattcatattacagtatgagtt gtaggaaacatgaaggaaagatttcccttctagtcagttcttatatttatcacttgtttcatgctccttggtgtg tatagtgttttcaggtgcaggaaatgctctgggaagaatgttgcttcttagaacgtaagagaaagggaaccccaa gggaagactcaaatgaagtccttaaaaagcccacttagcttcttctgagacagcattgtccaatggaatgcaagc gagatagttaaatttaaattttctggtagctatgttaataaagcaaaaaaagaaccagccaacattaattttaat aatatattttatttaacacaatatatcccaaatgttattttaacatgtaatcaatatgaaaaccatgaagacttt gatatttaaaattcttattttaatactaaatctttgaaatcagtgtatattttatacttacaagacatctgaatt tggacttagtcacatttcaagtaagtgctcattagttagtgactaccttagtggacagtgtagatgtagattatg aactttttaggaaaagacaatagcatgtggactccacaagaaattgtctttaacaactgtttgactctcataggt tagggctttagtgtttcagaatggtttctatggttgagatgtaaagttttttatttttataaaggtaagtacatt tttatacattttcattatacaagtaatgtcattgttgaagagttggaaaagtctgggtaaaagacaacaaattgt cctcacttcctcagtccagagataaccaatggtaaactttattgaatttcctctttgggaatgttgctttttaaa atcaaagagaaaatagatttcttttttgtcataattccttcctgttcacattcagacatacattattattgacaa ttcttttacctctttgtttaaatttatgtattctttttctcgggctgtatcctccaatagggtctctgtgagtgg agtactctggagcacagtaacctgaatggtgcttccctggattagggttaacaatagaaacttcaatggctttta tgtactcattgctaaaccagtgtagttgctttctattttaactatctgcgatctctttgcatatcctgcagcttt tttagttattgaggtataatttgcataccagaaaattcactctattaaatactcaattcaggccggcataatttg cataccagaaaattcacgctattaaatactcagttcaggccaggcgcaatggctcatgcctgtaatcccagtttg ggaggctgaggtgagcggatcacctgaggtcaagggttcgagaccagcctggccaatatggtgaaaccccgtctc tactaaaaatacaaaaattagccgagcatggtggtgtgcgcctgtagtcccagctatttgggaggccgaggcagg agaatcgctggaacctgggaggtggaggttgcggtgagccgagatggtgccatttgcactccagcctgggcaaca ggagcaaaactccgtctcaaataaataaataaataaatattcggtcgttttcattgtattcacacagttgtgcag taattatcactattaatccagaacattttcattacctcaaaagaaccctgatgctcattaaggagtcacttccca tttcctctttgcgcagtctcctggcaaccactaatctacttcctatctctatggatttgcctattttagacattt cttataaatggagtcatacagtacatggccttttgtgtttggcttctttcacgtaacacgatgttttcagtgttc ttctgtgttgtagcatgcatgagaacttcattctttttaggactgaatagtattcagttgtatggagggtaccac attttattcatcagttgatggactttggggttgtttctgctttttgagatcacattcattcttcaagaacattaa tttcttaatatcgtcctccattcttatcaaagctttcagtggtttcctgttgtctataaacataatagaaaaata tttagtttgcattcagtcttacaaagtgtaacttcaagttactttcttttccactcttattttccatcttgttgt tgtttactgtgtctaaaatatgtgctatatatgaattataaaattatgtagacaatatagataaccttgtcatct gatctaatttttaaaaagtgaatttgaatactgccaaactaaggagtatgtgtagtatggagaatttgagaaaga caagtatcagtaaagaagaaacagtatagtaaaacaaaatttttttctgccttggtccattgaaagaaatattta gtttcatataatgtgaacaaaaaccttaattttgaatgaacatttgatacataggaactcattcaaatcattgat tttctggttaactttgttagtagtttgtttttttctgagtcttttgttttaatccagatagtcagtctttttctc ctttttacatgcttgaaatggtaaaaatgaaatatttttacttattcttaaaaatatttccagaatgtcttacac acacatacacttagaaacaaatacaaatatatagtgatattctctcacattttacagagaaaagcgtagtataca tactttgttttttaatttcacagtagatcttagaggtcttttaatattagcatactgagaactttctcatttttt tcataatagtattgtttattccattgtgatgaatatgctattgtttttgaaactagtcccatattggtggacatt taggttgtttccaggtccactattacagataatactacagtgaataagtttgtacatgtatcatttcatatgtat gccaatatttctgcaggataatttcccaaaagtgaaattgctgaatcaaaagatatatccacctgaaatttgatt acccatatttttaagcataaaggaagagataaagggaaataaatagaaaaaaaagaaactatagattttcaaaaa ctttgtttatagatgaaaaaatggaggattagaacttttaagtaattgtccatggtcatgcactccacagaggac tgggcatggtgggattcggtggggggggcggggggctgtgtcaggatgctaacccaggtgttcctgattttaaat aagctgctatccttcctgttatattcatactgcatgttatgagcaaaaggggagagaagtcgccaactttttgga gaaaaagaataaaatcccagactttatttttgggatcatctgaggtgggaagatggcttgggcccaggagtttga gaccagcctgggcaacatgaagagaccccctgtctctacaaaaaaaaaaaaaaaaaattaaccgtggtgtttgag gcttcagtgagctgtgatcactgcactctagcctgggtgacagaatgagatcctagtatatgtaggtatttaatg tatatcgaaggcagcatttcagattggtgggggagaagatggattatttggtaatttatattgcagcaactttct attcatttggggaaaaagccctgtcccttgctcttactctttacataaaaaataatttaaaaatgaaagcaaaag gtaatatcgaaggatgtcgtgaggttagccttattcaatagactgacctcagtgagtactgaactcatgaaagtt tcatttttttcccagtcactgtcttttgcattttccttaattgatttttaacatcaccttggattaatctcatag ctttcccaatatgtttttagttatgaaaagtctatttctcctcttgccccgtattaggcttgacctgtggctgga gagatctgtggtactgtttcatatgtaccgccctcctctttctctcctcccttttttacttgttctgaaaacaat gagtaaggttaaaaaattatttttcacaaacatgaaaaaggttgataatacgtaagttttgaatatgggggaaag ttactctcatactgttgttgggagtatacatttttgtggcctttttggagagcagtttgacagtatttatcaaaa taaagtacacaggcatcttgaatcagcagttgttcttccaaaatttagtagtacagatatactgacataacttta cttgaaagaagaatcctttttataacttagtgtttattgacaagagaattagcatttagagtcatttattattct aactcttctaccaaaatggtgagatgtgttgtaatttactggacgtttaggttgttcccaatttttttgtaagta tgaaaaatgctgcagtgaacatctttgtctgtgtgctacccagtcatcactgttgagaatgtttcttaatggcta tatttaggtaagtgttatttccctttatttcccctcttatactttaaggagcacaggagatctcagttttaatcg cagctcaattgcctaaatggatgtgtgacttaaaacaagctctttaaccttactgagcaggaggttctgcagata tgggcctctcagactattttatagcagtaataaatggactacctattgtcacattcttcatgatcctgtagggag tatgtgtcagtaggacaacccattgagtgacagtcctaactttcttggttcctctccctcattgtgaggtcattg tttcacaattgtatatacttactgattcaaaaaacaactccaacacttattgaggggtagtactgttcccagttg agaattactgttgtaaaaattttggcaggaattatataagattattcatgattgttatcgacttatctttattcc ttcatttttttttaaagcaggaagcaataatagtttacgttcgttaacctcaatatctactctaaaacatgttgt ttttcttgttattaccatgaaagtctgggttaacaggagacgagactagcttcttcacagatacatacattaatt tgacattatagaaaaatgagactgtatcctgacttgccagtttggttttgattggtggtttcaccttttttttta gactcaggaaaacggtggcaaaaatagtataaagggtaaattctcatatgcccttcattcaagttcccaaaattt aacatcttatgcagtgacagaaaaattatcaaaatcaggagattaacattcatgtaatactgatagtctacagac tttattcagatttcacccattttctcaccaatggcctttctctggtccaggatcacaatatgaaattattcacgt gttgtattttagttatcatgttttcttagttttctttaacctgaaacaattccagtctttctttgccttttgtga ctgttatactttggaagagtactggtcagtaattttgtggaatattgcacaatttgtgtttgtatgttttcctat gattcaattaattttaagcatttttgattaagaatactgcaaaaatgattttatgaatcttatttagaggcacat gatgctgatttgtccagttatcagtgatactaattttcagttcctttcgttaaggtggtatcaggtttgtccact ctagttgctttttttcctttgtgattcataagtagtgttgattcagattctaggttaagaaaaggaaaaaaaaaa gtatcctatgggaaggtacttaaaaataagtactttgggaccaggtaattaacatttctcaccatactctaccaa tttttaacttccttgctgttttttttttcttttttttttttgcagtggcacgatcatgagtcactgcaagcctca atctcctgggctcaggtgatcctcctacctcagcctgttaagtagctgggactgcaggtgcacaccaccatgcct ggctaatttttcatattgtttgtagaggcgaggtttcatcatgttgtccaggctagtctcgaactcctggactca agccatcctgtgttggcctcccaaggtgttgggattacaagcgtgagccactgcctggccccagactttaacttt tattgatgattttgtcatggtgattttctgtttccatcatttcttctgtatttacattagtggtttttggtaagg aagaactttcccttgttatgtatttatttaatgttttatgtatatcagaatggactcgcggattttttttcccct cttatatcagatggacttacagattcttctatggatttcatctattatcatcattatttattttggtgctctaat tatcccaggtttggccccatgaagcctcttcaaggaggttcctggtccttttgacatgtctccatcattttgtga gggcttaactttttttttttctttttgagtcagggtcctgctgtgtcactcaggctggagtgcgcagtggtatga tcatagctcactgcaacctggatttcctgggctcaagtgatccccctgccttagcctccggagtagctaggacta caggccaggtatgtgccaccacacccagctagttttcgccttttttttttttctccaatagagacagagtcttgc tgtgttgcccaagctagtcttaaactcctggcctcaagcagtcctcccaccttggtctcccaaagtgctgggatt ataggcatggaccaccacacctggtcagaaggcttaactttctagtagcgcaaaatgttctagtctcttcttgtg cttttctctgccccagccatgaaatcagccatttcaccaaggaatattgattccttttactggaaatcggaattc ttccattccccctgcccccagtacttgtatcttaccatctttttaaaagtctttgtgtcttagaaccgtgttaga tgatctggacacattaccctgatgtacagaaattagtttatacacggtaattctttttgtagcagactgtttaat ttgtagcagattcattttgcactgattaatcctggaggatttcttcacaagttacattgtgtcaaatatgttatt tcatgggaacacaattgaattttttccttaattttgttcggtatttgataataacaatttaaaaaacttttctaa gagcttccttctaatattatatgatataggaatgtttacatttctcatttatttatttggtgtagtgatctcaga agtttttttaaaagaaaaatcttgactttgtctcaggtactctcagttcacttccatgtgggagggtcatttgcc atccatccagattacctttttgattaggcttttcagtgtctataaatatttcggtcttttctgatcatttaattt ctaatacatgcatatgctcctatgtataataaatagacaacttcaaatttgcagtttctagatggttggaaaagg gaaacattgtggtgtgtaatttatcagccatcagatcctagatatttgagattttaactaagcaaggtattagat agaccatgttgttttggcttcacagaattcattcatattgtgcattacacaatcagtgtgcatattgcacattga ttttatctgtaagttgtctttatcagtggttctcaaagtgtggtcccctgctagtatagtatcagcctcacattg gaactggttagaaatgcagacttctcaggatccacctaattgcagtagttaattttaacaagcccttcggtgatc ctgaaacatgttacagtttgagaaacactgctataatacgtttcatttaaattgtttcaggttgtgggggtaggg aataagactaccaatttattcatcttctgtgcaatattacctgtttacctaactcttagagatattaagatattt tgaagaatgtgtcccatgagattataatggaactgacaaattcctattgcttagtgatatcatagctgtcatgaa gtcttagtgctgtaccttactcatgtgtttgtggtggtgatggtgtacacaaatcttctgcactgccagtcgtct gaaagtatagcacatggccgggcgcggtggctcacgcctataatcccaacactttgggaggctgaggcgggcaga tcacaaggtcaggagattgagaccatcctggctaacacggtgaaaccccgtctctactaaaaatacaaaaaatta gctgggtgtggtggcgggcacctgtagtcccagctactcaggaggctgaggcaggagaatgacgggaacctggga ggcagagcttgcagtgagctgagatcgtgccaccgcactcccgcctgggtgacagagcaagactccgtctcaaaa aaaaagtatagcacatacaattatgtacagtacctaatacttgataataaaggactgtgttactggttgatatat ttacaatactgcactttctgttgttactttagaatgcactgtttgaactttaaaaaaataaattcactttgggag gccgaggcggacgaattgcttgaggccaggaattcgagactagcctggccaacatcgcaaaaccctgtctctact aaaaatacaaaaattagctgggtgtggtggtgcacgtctgtaatcctggctacttggaggctgaggcacgagaat tgcttgaacctgggaggcagaggtagcagtgagccagtatagcgccaccacactccagtcttggtgacagagtga gactctacctcaaaaaaaaaaaaaaaattgcaaaacagcctcaagaaggcccttcaggaggtgttccagaaggca gcactgttgttgtaggagattacagttccatgtgtgtgattgcctctgaagaccttccagtgggacattatgtgg agatggaagacagtgatattgatgatcctaatcctgtgtaggcttgggttattgtgtatatttgtgtcttaaaac agttttgaaagttaaaaaaattttttttaaaacaggaaaaaggcttataaaataaggatataaggaaagaaaata tttttatatagctttactatgtgttttaagctaagtgttattacaaaacaggcaaaaaattaaaacgttcataaa gtaaaaacgttatagtaagctgaggttattacggaagaaagaaaaaattttaaaataaacttagtacagcctaag tgcacactgtttataaagtgtatggtagtatacaataatgtcctaggccttcatatgcatccaccccaactgact catctagagcaccttccagtcctgctagcttaatttatgatacagatgcaccgttttacattttatataccttat ttttaatataccttttatatatttagatgtgttaatatcattgtgctacagttgcatacagtattcagtacagta acatgctgtacaggtttgtaacctgggagcaatagtttataccatggagatttgtgtaaaagcactgtgtaatat tcacacaaagaagaaattgcctgacacatttctctgaatgtatctttgtcatgaggcgatgcgtgactgtataat ttgagccacacatgttattttaaaaattgcaatacccttattaatgtaaaaagcagttgaattaattttaagata tttagcctgatggagccatgatattaccatttcaacataatttataaaaaaaattgttattttagattctctttg taccaagtttttgaaatctggtagatattttatatctcaatttcaacactaactttttaccggaagtaattgatc ttcctttagatttcataaagtttatggttggaaaagtagattcacatatctaaatcgtttcagacacacttaagt atttcgctacctgaattaagtaccaaaaaataatttttctgtaatatttgcatctaaattggtaaaactggttca tgttttttagtagaatgtagtagactttgaagcaaaactgtatccatttcaaaattgagctactgaagttaagca aattcatgaactcttgtgtcaactccgtattactgacattatattcaaaaggagattggatgttaggcaaatcta tattaaataaagtgtttttcaatgatttttttgtaatttttgcagccactttgcaaaacactgtcaattaaaatt tatatatgttgatttatgttagaacaaagtgtaaaatcttttttattggtttgtgttatgaaaactttaaatttc aatgtaaatttataagaaagttcttataaaatttctggcataccttcttccagtttctttttattttactttttc ttgtgttagcctggtcataaataaactctacctttctttggtgcttcaagttaagcttgttcataggcattgaga tgtaagtgaggaaacaaaagaatactgccatttttgtcttttattacttaatagtgaaaagcatctctttctaaa atcagagttgacattagagtagatctttgtgaagttattccactatacccagtgagcaaagaacacagatgtttt aaatgtattgttttttatttttttaacatttccatgaactggctaggattcatatttttggcatgtacatactga acaattcaactctttacatagggcttttcatagtctgtttcagaaagctgaacacagatattttcaatgtgtatc atacagtggaataaaggaataggagaaacatcaatttttgcttttaaaattcctaacatagctggagctgtctgt tgtgatagaaactaattgtttaatacctagctgaaattctttgacagaggtaaaggattaaaaaatatctatgcc actcttgattttttttttttttttacagctacatagtgacaactttttcttaagtttagaagttctttcaaataa atttcacctaaaagatttaattggggccgggcacggtggctcatgcctgtaattccagcactttgggaggccaag gcaagtggatctcttgaggccaggagatcgagaccagcctggccaacatgacaaaaccccatctctactaaaaaa tgcaaaaaattagccgggcgtggtggtgtgcatctgtaatgccagctacttggaggctgaggcaggagaatcact tgaacctgggaggtggaggttgcagtgagccgagattgcgccactgcactccagccagggcaacagagcgagact gtctcaaaaaaaaaaatttaataaggtagtgtctcttatatcacatggctcatctaaagctaaagggaaatagta atttttcacattttgaatctgttgatttttcgtattattagaaaggtcttccatatgatggatggtgtcttaatt gaatatcttccacgtgttgtaaagtatctattttagtattcctcataattttctaacaaaatttctataattgaa ataatttctttaccatctctttatgtaaatgtgattttctttcttttggcgcaagaatttaagccagtttatagc tgaccagagttacaagcccagatttgttaaaaagcttttaaacacgtttgttgtacatttacttctgacattgtt tggctaattttgttgcttttcttctgattgtagagcagaaagttcttataaaattcactgtgtatttgttgaaaa tgtctcctaatattgtctactttacttgtaaaactttaaaacccaagaaatagcttttaattttgctctgtcaca gctaattgtaattgtcattcattagaaaatttctagcataccttcttccagtttctttttactttactgttctgg tggtagcattagcttctgtatctctacttgattgtgtattgctgtaatgccttctttttacttttaaaaatgtgt cctcctcttgtccattcatttttaaagtaagaaaattaattatattcaaaatattaaaattaaaaaaaataaaaa gtattgcgattgagttaccagttgtgatttacataggcatcactgcaacttgtgttatttgtgaaaaacgtattt aagtaaacgcagtacggtgttagattgatgagtagaaaaatactcattctaactgtaaattagttaatttttact gactagatcagtatttttatgtgtaatactagaaatgactcactgtatcctgagatgtggagtataatatgcagt acagtggtcacagtgaaatgtagtactgccaaaagaataaaactttctttagtagaaggacattttacacagctt cagcttttaaatttaaattaagtacaactaaataaagctaaaaactcaattccctggtaacactagccacatttc aagtgcttagtagtcacatgtggctaatagctaatatattagaaattaaaccttttgagagttggttatgtatgt gattaaagtattttggttacttgggactagagattacagtcatttttgatcaggctgatgtcataggaacagtac caaggggacttctgaatcaagcaccctagaaagagctacttagaactacttgcattttctttgtggcatctctta taggcatgaaaaaatttcaaacatttttcatggataaaaggatttaatcagaaaagcatttggatatacatattt gaaatcatagcttgcctgtatgttactagagtagttggagagtggaaaagtatctatattctaaagactgtttca tcatttgggtgaaaaaccaaaaaaagagagtaattttgtttaatagctctttcacaaataaaaaaaagaatgttc atctgtagagacctatcgagatctcatcaggtttacaacaaactttagattagcaagctcaatttcagttgacat ggattgtggaaagattttcagtggagctcagatatttaatatcctgaatttggatgaatatacaatttctaaaat taagtacttcaactcctctgtggttttaaaaccagtagtggccatatcttgactagttattaaagcaccaacctt tttttctttattactttgcttttatcctttgagcaagttaggcaataagttctccatgtttttgatttctacaga agtaaatgaatgttattgctgatgtttatttcattctgtcttgatctcatgatatgagaatataaggagatgtct gaaaacttgttttaaaaatcctatgaagcataagttcatgaataaagaaggaagtggaacttgtcttgagagaat ttgcctatttagatggtttccaaagtgcattctgctagtcactagagatggtccttgaaagagttctgtaattaa gtttgaagaaacactgtgtaccataataatggcctattgaatattttaccatgcacattaatatatcaaaggctt tgagatatcttacaagaaagaaacttgctaaagcttgtcttcctgcatgtcttaagcttcttccacagtgacttt aagtcggacttgtctatcctgtgagaaatgctgccctctagaggcacaaattcaattgctcttaaatttgagtac cataatgctgcctgatcttagtgtgaagttgtgataattaatattaatcaaatgcatggtaacaaaaccaaatag aagaaatcttatgttgcttaattttaagaaaatatgaggaaacagaattgcaatttataaatacccactttttaa attgtacatgtgatatttttggctgctaataatcttttgaatacatttctgtatatggggaattgcggacacata gtacttcaagtctccaaactctgagtctctcctgctagcagaagccatccttccctgtgttaacaaccttgctta agacagttaccatccatggggaagctagtttcctcataccccttcccacgatactcatgtttccatacctgtaac tggagtcagactatatcatgctgcacctttgcagtcacaatgggaagaaaaggattatatgtaatttttttaaag ttttttttttttttttttttttttgctgacaaagcagcaaaaccttcaggaatatgtacagaaatgggtgatgat gatgatgtgagagagaaagaaacatgcttttgcatttggctgaatgtattggtatggtgcagttatcagagattc tgaattcattgttctcgttcaagtagccaggagtggttctaattgtttgctcctttggtctcagcattgacttat actaaatgaagttgaatgcctaaaaactcatagcataatatagaggaagaagtcaaaagattaaaggaaatagaa ctgatggagtggatttatcactgtgaccattcacttacctcccctattccctctccagtattctctactaagagg tctggaagacacttcattcaccaacctaatgagaaatactttgagggcagtgccagcatatatgaaaagtaattc aattgcttttgtctgtaggctggaggagatactttcattgaaattgtcttttttacttcagggggtgtggccaga ttctaggtaaggtccaggcatcattttcataaggtagcttgacaggcatgatgatcagagatgcactgacttgaa ttctttttggctgtgactaaatgatcatggatccctacgtctaaaatagagaggaagcctattaagatcctactt tatctgtatgagcaaaaaattttaggtctcataggagaaggccttacttgaactaccataagagtgagttggcca gcctgggcaacatggtgaaaccctgtctctacaaaaaacacaaaagttaacttttgtgttttgggcatggctgcg tgagcctgtggtcctggctgctcaggaggctgccccctttttgcagtttacttcatgggttatatgagaaaacag gaaggagggggatatcaccgagagattctgatattggagctgtgtgcaataactgataagtctctggttcttacc tccatatgaggaacagagagagtcttgtttgtacaaggcttagttataatctgtcaggtttgaggatcttgattg gaagcatagacttgatctgtatttgtggtttctcatttcaaatagcctctcaatgttggataaatttgcatgttc atgttttatagagtcagaactcaatttcctagtattccttgcagctagggcacaagcatatgacctaggctttat tgatcagatgcatctgtgtaagacatgaattcagggtcagagaaatgcagggtaacagctcttgcctggaattgc tttctaatgtgcatgttgagtgacaagatattgagctgtttagaggtagcagtgacagagatactagtgagcctt gttgtcctaggctcagctttcatggtactagggctgaaggatgggaacaatttggtggggagacttaggtatttt tcctggcatcatatctccaagtggattcttcagttctctgataattcttggaatcccctaatatattaataaatt tattttctcttgaaaccacctcaaatagattctgtttatagcttagagccctgattgatcgtctaactttaaaga ttttatttatcgtcaaaatattttatttttctaattgagatctaaaaattgtcaagcacttatactctatttttt cccttcctagagctctgcaatcctttgctgtcattttttcttaccaggaatgccaatttttgtaccttttgaatt gctttttcttctttgctacttggcttgagctccactattgttatgaaacctttcttataccatcccagcagcctg atggatttttttcttcctctgaattccagtagcacttgtttagacattaagtgcaaggtgtttttttgttttgtt ttgtttttctgtatgcatcttagcttccgcactaaatgataagctccctgaaaactagtactatgcctctttata tcgcttgcattgtccaacagaatgtcttgcacctattagattcaaggttcagcacagtcaccctcataatatata gtgatgagagttgctgagaatcacagtgatgccaggctgaatgagctgagataataaactagttagatgcatagt ctggccatgaggcccaatttccatcttcctcaaggagtctgagacttgcctctcagtagttttcgttagtaggtg atcatatcttctgaattactgtcttcttaatgttttatgagtttttttttgccttgacaaaagccttctatttct ctcatgcttcctagttccttccattgtgccctctgcaatttcttttcactaagcttactatttgcagtctcttaa aatttgtgtaaattatgaaatcaaagatacaaagaaaggacagaaagtagtataacaaacacctgtgtatctaac ctcagggtcaagcagatattaaccttttgcctcgtttgatttccttttaaagttcttccctccccatacttttct cccttctttggaggtactcattatcctaaagtccttacgattatttccctgctgtttttgtacacataaaacatt tacacaaaagatgacctgtgtttttttctagatgtgttcatattgacatgtgtagatttagttcattcattgtaa ctactgtttagttttccaggttgaataatgctaagttttatagagataatgcagttctaccttttggcaaataat attgtaatgctcgtacataggtacttactgtaattttatctaggatagatacctgaaagtagaattgctgaatca cagggttcaggtgccactttatcaagtattgccgagtccagtccaaagtggagctaccattttatacccattaac atgtttccacacttttttttatcaacatttaattttatctagctatgatttttttgcctatttgaaagatgagaa atggagtttgatgattttcattttcattactttgatttatagtaaagttgaatgtctttgcatagtttttgatca ttctagtttcctattctgtgaatcttttatgttctttgctgatttttttcctattgggtattgaaccttaaattg atttgtggtgattctttttataatctggatattaatggttttggtaatatgcattgcaagtatctcatagtgtgt gtgtgtgtgtgtgtgtgtgtgtgtgtggtttttttttttttttttttttgagatggagtctcactctgtctccca ggctggagtgcagtggtgcgatcttggctcacacaaattctgcctcccgggttcaagtgattctcctgtctcagc ctcctgagtagctgggattataggtgcccgccaccacacctggctaatttttgtatttttagtagagatgggatt tcactgtgttgtccaggctggtctccaactcctgacctcgggtgatctgcccgccttggcctcccagagtgttgg gatgacaggcgtgagccattgtgcccggccttcgtgtcggtttttaacattgtttaaaagtttcagatttactac atgagttcatttgtagaatctctcttctgtatcatagcttctttggtctgctttcatgtctttacaatattgtgt taattttaatagttttgatataaatcttgttatctgataaggtgaatcttctctcaatgaaaattttgggtcagc taatcatatttcaccctgaccctaatgaaaatttaacagaaaacaaaccctgttggaaatgtgattggaatctca ttgaatttacaggttacagtggggatagttgtcacctttacattggttgactgtcattgatatttctgttcaata cagttctatttttttcttttttctttttcttttttttttgagatggagtcttgcttttgttgtgatctcggctca ctgcaacctccgcctccctgtttcgagtgattttgctgcgtcagcctccccagtagctgggattacaggcgcctg ccaccatgcctggctgatttttgtatttttagtagagacagggtttcgccatgttggccatcctgaccttaggtg atctacccacctcagcttctcgaagtgctgggattataggcgtgagccactgcgcctggcccagttccatttttt tcttgatagaagtcttcattagattttttttttttaggtttttatgatttttgttaccatcgtgaatggcaaatt tttataaaaatactttttgttactggtagctacaaaccaattagtataagagacaaattgctaagaagcaagttg ctgtttgtatcttgattttgtatctctcaatcttgctgaattcttagttgtttttgtttgtttgtttgaggtggt gtgtcttactctgacacctaggctggagtgcagtggtgtggtcactgctcaccacagccttgactgcctgggttc aagcaattctcccacctcagcttcccaagtagctgggaccacaggtgcatgtcaccacacccaaccaattttaaa attatttgtagagatgaggtcttgctatgttgcccaggctgctctcgaactcctgggattaagcgatcctcttgc cttggcttcccagagtgctgagattacaggcatgagccattgcacctggcctaaactcttacttctaatgataga ttctcttggatacctcatgcagataatcatataagtagtgaataaagatggtttttgttgctttctaagcctatg tctttatatttttcttctgttattgacctatgtaggaccactttactaaaattgcagcttttcctgggccttggc tttatgtgtgtgttaatgtatggagtagaaaggggtatctcagaactgaagtctcccactaagggccaaaagtct catttctagtccctacatatgagtcaaaggataaatataggttaagactagcaacccattgtagggaagaaacat tgccaacttgtagcctaatatatatatttttaatatgttactgttttttccatgttactggcacacacttaaaga tttctgtttcatggtggctaaaggattaatttaaaataattttttttttcctttttgagttgagtttcactctgt tgcccaggctgaagtggagtggggtgatctcagctcactgcaacctctgtctcccggtttcaagcgattcttctg cctcagcctcctgagtagttgggactacaggtgtgtgccaccatacccagctaatttgtttttttttaaattttt agtagagacggggtttcaccatgtaggacagggtgatctcgaactcctgacctcaggtgatctgcctgtcttggc ctcccaaagtgctaggattacagacgtgagtcaccacacccagcctaaaagaattattttgatatatcctttctg catgaaatttctgtgggaaggcttttcaggtgatctgatctgatacagtgttaggactggaagtatagcttatac tatataaatttccttaattgaaatttcacattatttaggaactctgtaccctgaagctgtctcactctggtattt aatgtcctacaattagtttaagagagcaagcttgggtaagtttcttaaaacttccttatatttgtttcttcatca atattagtagtatagtatcatctcctatcttatagattattataagaaataagttaataatgtaaagcatttaga acagtattagttattggtctcaacctccttttagctgttatctacagcctttctggtagattttacttattctgg gaagatagtggtgtattcctcataatctccttgtacattccaggtcctacaataattctcaaacattttagcatc cttacatagactctccaatatcctggtcttatttccctgatctcctcaaatctactgatttttctcttcctcaga caagtgatttttcacttatttggtaaaatcctaattctggattaactcggttatcagattctttcattcctatac cacctgaatgctgcagtaaaaagttacctcaaaggacatttaaaaaaaattataaattcatgattgttgatcaca gaccctaatactgcatgggaatccactgcattatccttgtcagctagtttcctcatctttaccatgactgtttca atcatctgttctcctcagacctccaacatgccctttctctttctacagaaaacttttcctgttactttccagtga aaaccggagtacttggtgcaacccttagttgcccactatcttttctatattttctttcttcttaatgttaaaata gaggtagtcttgctttctatctaaggctttctccctttctctgcagtaccttatgctgttgatgatttccctttt ttttgtatgtttaacttttctttacacttgattcttctcgtcagtatttaaacacgcttaattcctgctattaaa aaaataaaaacaataagcattaatgggtaccacattactgacctttctgttctctttcctagccaaatgctagaa attattgtttccatttctcacctttgactcacctgtttagtttattcattccactgtaatagtatttgcttatga taccaacaacctctctgttagtaaattaagtgaatatttttaaacttggtcttatttgatctctcacttggtgtg ctagggcttctataaaataccacagactgggtggcttaaacaacagaaattaattttctcactatgtggaggctg gatgtcaaagatcaaggtgttggcaggtttgttttcttctgaggcctctctccttggcttgcagagggtgccttc ttgctgtgtcctcacttggtttttcctctgtttgcatgcatttctggtgtctgtgtccagattggattagggcct ggaagatgaatttactcacctctctaaaggccctatatccaaatacagtcgtgttctgacgtactgggggttagg gcttcaacacgtgaattttgggggagacagtttggcacataatctcctagcaggtttaaacagttttgaaaaact atccttggctggacgcattggcttacacctgtaatcccaacactttgggaggccgaggcgggtggatcacctgag gtcaggagttcgagaccagcctgaccaacatggtgaaaccctgtctctgctaaagatacaaaaataagccaggtt tggtggcacatgtctgtaatcgcagctactcgggaggctgaggcacgagaatcgcttgaa cctgggaggcagagtttgcagtgagccaagattgcgccactgcactccagcctgggcaac aaagcgagagtctgtctctggaaaaaaaaacaaaaaacaaaaaaactatcctttaaactc tttggttttcattttgcctctctgatctattgctcttcagcctttttttaacaggcttct cttctgcttggttattaaaagtagaaatttcttgagtcttggtcctttggtattttctgc ttctcttacttctgttatttctccccaggcagtcttaccactttgatttccatttatgaa ggaactgcatacctgttgttccagcacttaatttgcctgagtttcagacctatttaataa tgccatttagcttatcctagaggtaccttaaaaaaacgagttcaaatctgaactcataat aatccccatccatgtgatttgaccctaatctacctccttagccttactattctttgttac tctttgccatagctgcactatagcaaagcacggcctcttggtccaggcgtaatatacctt ttcctccagagtctttgctcttatttcctatgttaatatgctcctcatttttcatttaca acattacccagttttcaatacatacaaaagataaaaacattagttattagggaaatgcag acttaaaccataaaataatcactaaaaccaactagaaggggtaaaattaaaaagactgtc aactacaaatattctaggaagccatccagaacactcgtaaattgctggtgtgagtataaa atgggagagccactttggaataccactggcaatttcttataaatttaagcatacaccttt ggatccagcaattctacttctaggtgttttcggaaaaggaatgaaaacatgtccacaaaa aacttttaaaaattatatttacagtcgatttactccttacagctgacaactagaagcagc ccaaatgtcaataggattaatgactaaaaattttttggtatattcatacaatggaatgcc actcagtaataaaagaactacagaatggcaaatgtacacaacatgacatggatgaatctg acatacttggcaaaggaagctggacatgatactgtattattccatttatattaagttcta gaagaggcaaagctactctggagtgaaaaaataaaaacagaacaaaggttgccttttgct tctggaaaatggtgatattcaactgggagggtgcataaggggccttcttgaagtgatgaa aatgttccatatctcatagaggtgtgtgttacatgggcttatctattaaagttgtacagc taagaagtatgtatttcaatggcatacatgtatcttagataaaataaacaagttttatct aaaaattatagaaataattgagttaggtgacagggagtgggaggtgtagatgaaagaaga attgcagaacgttggtggttgctgaagttgagtaatggatgtggggaagtttagtatact attttgtttgttttttgtgtgttcaaagttttccatataaagatctttttaaatcaaagt tacgaagacacttaaacagcacctgtggtctgactgccagcttaataaagaatgcttact aatgtaattttcttctctgtgaatcgtaccctgattcagcactcctttctccccaccagc cactatcctgaattcctcttgactttttaagacatcttccaaatatcattctaaacgtca cgtccatgggggaaagccttttctgattcagaacactcactagttttaagtgcccctgtt aagtcctttcatagcttttatcttaattatgatttatttgcttaactattatcaacctct acactagactaagcctaagtttttggagggaaagatatatctattcttgctgttttaatg tctagaataataatacatttattgaatgattatgttggtgtagttttttaaaaaaattga aggatacccaaattgaagagctattctattactgcttatgttgtaaggtcagtatgttat aaaatgtatgaatgtgagagaatgactgagaaatgataatggtttctttctgattttatt ggcatctgataaaaacaaggggcaataaaactgtgttattagatctggaactatatccgg aaagtcctgtcagttggttctcaaatgcccttgagctttattatgcttcttccttattaa tgtattaagccgtgtgagtgaaagacccctttcctctttacaaagaatattctaaatcac ctcttttttctctcttctctaatgtgcttcttcctttgactattagttttggtatttatc agggacagcgatctttaagagcttgttgttttgttattttttgtactgcttgctgtatgt tttaaattttgatatatcttactgccaaatgaaggtgtgagctcataagtaataaagata gaaaaaatctgctttgcttataattataccattaaacatgttgtccttaatgcagataca cactattggtattgtaggtgcaggattatgttttgatttttgccagttcatatttccatg gagtatttaggcaattttaacaacatagatttctgtaatgaatgtttttttaacttatgc tgctgcttgagagacagagtcagactgccagggtttgagtcctatgcccaatatttccta gctctgtgacttttgagttatctgctttttgcctgtttcttcctcattaaaacagagata ctaagtgtaccttatagtgttgttgggagggctatttgaggtagtatatgtaatgcctgg tacttattaaatgttagatcttttttactttcttgggttctagggagtccaataagtaac aaatttttcctctaaggtttttttctttattggtggcaaaatatacatagtataaaattt acatttcaaccatttttaaatatacaattcagtggcatcaagtacattcacaatattttg caaccatccatctctgtccatttccagaagtttttcatcatcgcaaataaaaacttgata cccattaaacaataactcccatctttcctcccctgatttcctggtaacgtatgttatact ctgtctttatgattatgactattctaggtacctcataaatagaatcatacagtgtttgtc ttattgtcctctcatttcatttggcataatattttcaaagttaattcatgttgtagcgac ttctagttccattttttgtggttgaagaaagtacttccctaatagaaatgaaaacatgtt tgcaaggctttttttaaagaatgcttatggatttagtcataataggtaacaactgaaaac attggtatggctgggtcaagttggactaattcactgtagcccagggtcatgtaagaacat ggagttttcaaggtaggcatgggggtggtattgaggggagggtagtaagcataaaagaca gtgctcagaagaagataagggtgccaggtttcagttccagggatgttcacagtaaggatt acagtgggctaaattttaagattaattttataaattctaaacacatcctgttgactttaa ctgcaactcttaaaatgtatcaataacatgactttataattcctggaaactgatattttc ccaatactttgaagaggtttaaaaatagttgactaatattagaaccaatatttcatgtat tgatttttctctttaagtgacagttagtttagaaaatgctaaacttaagttagcagtata gagtaggtatatagttatactgagtatgaatgtcttggtaggcttggaagcctaccattt atgtcctcttgaggtacctgaattaccaaaagctttatgtattctgaagttattgaaaat aagagcttttgggaattcaggtagttcaggagtgacttttctaaaaaacagaactgagca ccatacactacttcttatagccttttaattactgccaattgcctagaataaagttcaaat tcgagggcaacacaaacatggccctttgtgaactgacagacatttcctaataattcctta catacatgatacccttgagccttacagaattaagaatttctgaatttaccttggtgtttg atgcatctgtgtttctatacgtgctgtttatttacctaaaatagactattctctatttag cctcaaaagtctcatggaaggttttaaaaaatcctgtctaagctctgttaattatattct ttatactattatatttagtagttaatattataaactaccttacagtaatttttacagata cctggcttactccctaaattgagttgcttgagggcaggaattgtgtattaatctttgctg tattttctttgccttgcttactgtagttgattggtgctaattgaataaatgaacatttat gcttttctttttgagaatattatatgggtagttcctttagttgtttaatagaagatagca cagacttctatacagattggtttttatgggaaaacacaagagaataattaattaaaacat aatttcatctagaaatttctgcagttggagcgaatattttttaagttattaacttaccta gagtggaagattgaatttttgaatgatagtgttttagttattaaagtaaatggcatgtaa gagaacttttaagttcatgtggcaaaacagggaaaaattgcaaagttagagtctgtaagg tttctgatgtttcatagactaacaaaaggttgctttattagcagcattaatgaacgaatc gctttattggcctaggcatactggtagcattgtcgttccaaagagctgagagttcttatt tcaattcatgattatgttagacaagtgaagtgaaagccgttgaccttctcttaaggagaa aatagaaagtttttacaaatagattatagattaaagccttttaaggaacatgatgaagaa ctaaattactgctaaatcacttgaaaaatttatatttttttatgcaccagtcttataaca gtaggttttttatagacctattaattgatatttaaaataataactttaataggatgattt tgtagagttagtcaaagtgattgacaaaagcaaattattaaaatagtcaaactttcaatt ctccaaagaatgttcagttttaaaagcaatatgagatacaggaagtattaaactggagat gtaatgccacttggatggcatacttttaacattattttttatttgtttaaatggaatgca ttttgcttattagaatcattttggaagtgccaatttgcttctgttaatttacattaaaga ttaaaataggaaccactggtgaccttctaaatcaaagttcttcaaaacggttgttttaaa cagcaagaattacagaactaagtattgtatagctattaaaactgaaaattatgtagcagc agtccattttaaatttaacaattttaaatgatacttcaaaatataaaaaatacaaggaag aatataagatgcttatgtatctgtactgccaaaatttaacaaatgttaactttttgctat cactgtttcagaactttttaaaataaattaaaaatataattgaagattactaatctcatt cttttgcctctcttctcagaggtaattacttttctaaaggtatcctgtaagttttcaata ctttttaccacatatttgtataaaaagataaacagtactgatttccttttaaaatgtgaa taataatatactgttggcataactttttaacttgcttttttcactcagtgtttatttaca tcaagatttattcatgttgatatttgtatgtacttaaagtttgtttattttatcctctga attattagtttggtccattatttatgatgtttagtttgattacttggttaaggtggtgtg acctgatttctctattgtaaaagtatccttttccccttgataattaatcattaatctgtg gggtaatgccttgagactgagtaaacattgtttctcaacagcctttcacccagtgttttt aacatccattattgatttatgcctgaattattggttacattgatgcttgcaaaatgattt tctacatctattattctttccacatttattagctggcattcttctttctgtaaaggagtt tctgtcctttcactaagggctcatggtttcttcattaattcggtgaactaaaatccatta ttgttatgtagtcatgcactgcatatacgacattgatcccatgagattataatggagctg aaaaatttctatcgcatagtgacgttgtagctgttataacatgttaggactaacgtcaca accaacatattacatgtttgtagtgatgttagtgtaagtcaaatctactgtgctgccagt ctaaaagtatagcacatataattgtgtacagtacataatacttgataataataataaaca gctatgttaacggtttatgtatttactatgctatactttttgttgttattttgaatgtac ttctacttattaaaaaaaaaagttaactgcaaaacagcctcaggcagttccttcaggacg tactccagagacattgttattataggagatgacagctctgtgcatgttattgtccctgaa gaccttccagtgagacagtatgtggaggtggaagacagtgatactgatgatcctgaccct gtgtagggctagtctaatgtgtttgtttgtgtcttaggttttagcaaaacagttttaaaa gtaaaacaggaattttttaaaatagaaaaggcttgtaaaataaagataaaaggaaagaaa atatttttgtgcagctgtacaatatgttagttttacgctaaatgttattacaaaagtcag aaaagtttaaaaactaaaaagtttatatgagctaaggtttattattgaagaaagaaaatg tttaaaaatagatgtagtgtggtttgagtgtatatttacaaagcctacagccgtgtgcag taacgacctaggccttcacattcactcttcactcaatcattaactcacccagagcagctt ccagtcctgtaagctccattcatagtaagtgccctatataggcatatgattttaaaaaat cttttatactgtatttttactgtaccttttctatgtctagatattctcagacgcaccatg ttacagttaccaggcagtattcagtacagtaatatggtatacaggtttgtagcctaggag caatgggctataccgtgtaggcttgtgtaaatacactctgtgatgttagcacagtgaggg aatagcctaacaaacacatttctcagaagatatccctgtggttatgtgacgcatgactgt gtatcctttggtgactcaaattgttaaaatttagccagcgagaactcaatcagtctggtt tctgtttctttgacatgccccctcatttttatagcaccagtttgctttgtggcacaacaa aacattctctccttaccttatggttttcttaccccagacctggacttggctattttacca aaagttcttgtttctcttagtggggaatggtatttggaagccaacatcatggatgcatta ctgggtgtcattgcttctattggttcttttcattagacagaataagaaaatatactttta aaaactagtgatatttgaattcctagtgacaccaaggttcttttctttccttgtttcata ttcctcttccataatgaagatccagtttaccaataacatcaatatatgtttattcatttg ctctgtcttatacatgatataacattggaattactatagcaataccactactaataataa tctcaataaagtttaagatttctatgcagttttttatttatagcgtatagcccagtgaag gtatatagtcagatcgctatgcttatagttattttaattatatttttcacataggcaatt tgaaactttttctaataggaaggttaagcctatttacttatcttgacataactagtatgt ttaatctaactgatatgttgtatttactgttgttttatgttatatatgtttttcttttac tcttttttaaaaagttatttttactaatacaaaaatcttcctttctctagatatttagaa tgttggtcttagctcttatagtttcccagtctttttggtatttttgttgtcattactatg tttaattttgttaactagaattcatgttttcttttcttcatccatttaattgggctttat actttttttttttttttttgagacagagtctcgctctgtcacccacactagagtgcagtg gcgcgatctcagctcactgcagcttctgtctcccaggttcaagtgattctcctgcctcag cttcccaagtagctgggattacaggtgcttgccaccatggctggttaatttttgtatttt tagtagagacagggtttcgccatgttggccaggctggtctcgaactcctgacctcaggtg atccacccaccttggcctcccaaagtgctgggattacaggcatgagctaccgtacccatt ctatatattcctttgcataaatgttgtgttctgaggaaaatttgtgtctgagcatttaat aatttaaaaaattaccatgtgagtggatttattttatatgaataatgtgccacatgtaag ttgttctgcagttgattttccctttttgtggagcttttacaatattgttagtatgtttac ttgtccctttttccccttgtatgtgtagccaagtaattataaaaaatggtgcactttgcc atatctgagcttctctttttcccctcaagtagatactgtgcatttgaactgcaaaaaggg tcatattcttgctttatctataatctcttttaaaggcaaaggctgatggttactttttct ttaataagaatcttgatttttcattttgtatatttgatatttatcattttaagaaaaaaa aatcctctatacatagttgttagaaatttatgtagaatggatgttggaacccaatcataa ggctgtaaaaccataaaataagtagtaataaattttaaaaaatgaataggatttgtatga agaaaataacaacacttcattctaggatcaaacaaatgaagagagagctatttttcagaa tgaaaaaccaaatagtgttgcatgtagactctattcaaagttctagtctgtaaaattaat agcaattctagtcaaaatagctgttttatcttttgagaagttcgggaagtttgacaatct gattgtcaaatatatgttgtagagtatgttaatgaaactatccagtgtaaaatttaaaaa taaagaccagtattgttatattgtactgtattgcttgctatactgtagtggtgtagtaat taaaacaccattctagtgatacaggaatagggtccaaataaataaaaactgagaaggaaa ttgtatacacatgcaagtgtgtgtgtgcacacacacacacaacacagaaagggttttttt ttgttttttgtttttgttttttggagacagggtctcactttgttgcccaggctggagtgc agtggcatgatcatggctcattgcagccttgacctcccaggctcaagcaatcttcccacc ttagccttgtgagtagctgggactatgtgtgccactgtttccaactaatttttgtacttt ttgtagagactgggtttttcatgttccctaggctggtcttggactcctgggctcaagtga tccttcacctcagcctcccaaagagctgggattacaggtgttagccaccacgcccagcct caaaaagctttttttttttttccctataaagagccatatatctaaaaagggaaacagatt tgactctattacaatggaattctgtgagagaaaacattttaacaagatcagactatctag tagaaaaatatttgtaacatatagcaaagttttaacaaacataataaagaccttctaaaa attaataaaattgaaaaaatgggttatgaataagtacaaataattcaaagagtgtacaca aattagttttcatagaaatatgcataaagatatatgtacaagtatgttaattttagcatt atttataataccaaataataatattccaacaaaatagttggaataatctgaatgttcacc agtagggaaatggtacatctgtattttagaatattagcaagctgattaaaaagaataaga tctttgtgaccaggtatggcaaaatatcccacattattcatgaaactatagaatgatctg tgtagtatgatctcatttacttagctcagtaggtctattgtttgtggtaaacttaaaatt attttatttatcttataggatagggacttttaagttaacctattaatattattaggaact aaaatatatcactgtaagagaataaagacaaatatagaataaaaggaactaagaaaaata ttttacatcagattaattggaaatattggtatgaatccatgagtaaataaagaaatatct ctccccccaccctgttctgtctaccagaaaatataagaagcatggacaatctcagtagca atggttacatgcatcttggttgctaaatatggctccccattgaaagaattcaagatgtct tagaaaaatagatgatcccagttcttaggaaaacacacagcaagccagaaaacaaggaag tggtgaaaaactgaggaattatgtaaaaaagaaccaactcaaaggagttctagtggccac ctccgaaataatgattgcaatcacaggcatatcttggagatgttgtggattcggttccag accatcacaataaaacaaatattacaataaagtgagtcacacacatttttgttttcctag tgcatataagttatgtttatacaatattgtagtctgttaagtgtgtaataacatgtttaa aaaatgtacatgccttagtttagaaatactttattgctaaaaattgctaataatgatcaa ctgagtttttagtgagttgttctaatctttttcctgctggagggtcttgactcgatgttg atgactgctcgccagtcagggtgatagttgctgaaggttggggtggctgtgacaattttt aaaaataagacagcaatgaagtttgttacatcaattattctttgatgaaatatttctctg tagaatgtgatgttgtttaatagtgttttacccacagtagaaattcttcaaaattggagg gcagttctctcaaaccctgctgctgctttgtcaactaagtatgtaatattgtaaatcctt tgttattatttcaatgatgttcacagcatcttcaccaaaagtggattccatctaaagaaa tcactttctttgctcttttttataagaagcaacacctcagccattaaaattttattttga gatttcagcaattcaatcatgttatcaggctctgcttctagttctcttgctatttctatc acatctgcagtgatttcctccactgaagtcttgagctcctcacatccatgagggttggga tcaacttcttccagagttctgttaatgctggtgttttgatctccactcatgaataatgaa tgtttttgaaggcgtccagattgatgaatccttcccagaaggctttcaatttactttgcc cacatctatcagaggaattcctatctatgacagctatacctttatgaaatatatttctta aataataagatttgaaagtcaaaagggcttctcaatccatggcctgctgaatggatgtgg tggtagcaggcatgaaaacaacatgagtcttctggtatatctccatcagagctcttgggt gaccaggtgcaatgagcaatgtcagtgagcaatgagtagtattttgaacggaatctttta ttctgagctacagtctcaacagtggacttaaaatattcggcaaaccatgttgttaaatag atgtgctttcatcaaggctctgttctgtttgtactgcacaagccagagtagatttagctt atttcttaagggccctacaatttttggaatgataagtgatcattgttccacttggaagtc accagtagcattggctcctaacatgctgagtcagcctatcctttgaagtttggaagccag gtattgacttatcgtttctagctaggaaagtactacatggcttcttcttccagtagagag ttatttcatctcaattgaaaatctgttgtttagtgtagccaccttcatcagtgatcttag ttagatcttctagataacttgctgtaacttctccatcagcacttgctgcttcatcttgta cttttatggagacagcttctttccttaaacctcatgaaataacctctgctagcttcaaac ttttcttctgaagcttcttcacctctctcagccttcatagaattgaagagaggactttgc actgaattaggctttggcttaagggaatattgtggctggtttgatcttctgtgcagaaca ccaaacctttgtccatattagcagtaaggctctttcttgttcctattattcatgtgttca ctggagtagcacatttaatttccttcaagaacttttcttttccttttttaacttggttaa ctggcacaagaatcctagcttttggcctatcttggccttcatcattccttcctcactaag cttaattatttccaacttttgatttaaagtgagtaatgtgtgactcttcttttcactcca acacttagaggccattgtggggttattaattggacaaatttcaatgttgttttgtcttag ggaatagggaggtctgaggagagggagagagatagggattggctggtggttggagcagtc agaacacatgcaacatttatcagttaagcttgccatcttctgtgtttgtgatttgtggtg gcccaaagcagttaaaatagtaacatcaaagatcacagatcaccataacagatacaataa taatgataaagtttgaaatattgtgagaattactaaaatgtgatactgagacacaaagtg agcacatgctatttgaaaaattgcaccaaatagacttgcccaatgcagggttgcacaaac cattattttgtaaaatccacaatatgtttgaagtccaataaaatgaaatgcagtacaagg aggtatacctataattgtaaaacctttgattgaaaaaaataatccatgagtccagagaga taagaagaaaaggaaggccctcctttagagaattgtagtagcagtgttagaactggaaaa ctgtactgtaaactccaaagaaacaatctgtgccaacagcatcaatggatgccaaaacct ttaggtgtaagagtgctgtgaacatgatacgtatgcagtggtgcccaattattctgtaaa tcttgtacgactacaaagggaaaatgtgcctttgcaatagatctatagattgccacttga actaagtggtcacacttataatgggacagcctgataccatgttccttttgttatgatata atgcggaatgatgtaaccttgggtgtattcttgcctaatgtttatctacaatccagtcag gtttctagacttgatcttgtttccaagaaatacaggaagttgtggagtaagtcagaatac attctgggaagcagtcagacaaatctaggatgtgggacattctgtaagaaaactggccat gagtcctgacacatcattgtcttaagaaaaagatatgtgtgtgtgcgagagactattcta ggacaggcgagaccaaagagatttaacaggcaaatgttatacataaattttcatttgccc ttggattgctctctcaaccctgaaggcttaaaaatagatatctttgagacacttgagaaa acatcaagatactagcttaccttatttttacttttttttgactagatgtcttaatggttt tattactttgtaagacttgttttaaaagatatgtttcactactcagtatagtctatgata aaatcagtaaatataaaatatttgtcatttgcatctcttaggaggttcgtctccattgat gaaagctcagcttatgtgtaactgtcttccaaagtagcttgcttggaaggacattcacta tcatcatattctgctttatttcatagctgtgtaatcacatctactacatccttgtttatc gtttattagattactgcttttaagcctattttagttctttaatctgagaataacagttga ctcagttaatttcattaaagcaagattgcctgtaatttttttataaccttagaaaagtac tatgcccaacagcaaaacaaaaacctaattcaaaaatgggtaaaggacttgaacagatat ttctccaaagaagatataaaatggccaataaacacatatgaagagtctcaacaccagtag tcattagagaaatgcaaatcaaaaccacatttagatacaatttggctattagcaaagaca cagaaaatagcaaggattgggaaggatgtggagaaaattggaaccatgcctgcactactg gtgggaacgtaaaatggtacagctgctatgaaagacagtttaccagttcttcaaaaagtt tgatgtagaatgattgtatcatccaacaatttcactgttaggcttatctccaaaagaatt gaaagaagggacacaaactggataacttatacagcagtgttcatagcagcattactcata ctaaataaaagatgaaaaaggccaaatgtctgtgaacagatgaatggacaaatgtgttta tacatatagtagaatattattcagccataaatacaaatgaaattttggtacatggtacaa catagataaaccttgaaaacattgtgctaagtgaaataagctagacagaaaatattgtat gattctacttagatgagataccccaagctggcaaatatataaagctggcaagtataatag aagttactaaggcctagggtgagggaggaatgagaagtttgcttaatgagtatagagttt ctatttggaatgatgaaaagttctggaaatgaatagtggtgataattgtacaacattctg aatgtactcaatgctactgaattgtccatttaaaaatggctaaaatagtacattttatgt atattttatgatacaaaaatatttttaaagtaaaagtttgtattttgatgttacttgtga aaaatattttcactaataaaattttgttgtttcattgctgcctctttgaaaatgtattaa aaattatatgcattttgaaatattacagctatataaatgaaaaagagtatcagtaaagaa ttttagctcaatactctcatatcatgttatttattgtcctttctgtttcacattaagata tacagagtcaacaatttaaatttgcagattaaaaaacactttctgcttttggccctatag aaataaaccaaactggaaaaggtgtcatgtttcctagattgtggacacagaaaaagaaga gctcaaaaggacaagtaactcagcacagtagatggttattgacctgatcatatttaagaa tatcctaaaaatgcaaaaatgttaagaagcaaaactgggaaatactgggatgatatatga aaaattcaaatgacaaatatgcagtggttgagtatttatgggtttttttggggtttacta ttttttcccccaaatttaatgtctcctcttttaaatacttctaccggattctctttagat gatgctgcaattggattgagacattttcattgtttggctttgtaagatttccaaatgcgt atagttgttaaaatacgtattcaatgagggtggaaggaaacacacaaaaatagaagtatg tcactctggtaagagatgataggtccgatgactgctaatacttttccaaatggccttatt tggatccttcttagaacttttatatttttctaatattgacctacccctttggaactagag tcccattgtcaagaactgtgaagaatcttgacattttaccctacttgcaagctagcagtt actcagttgtagttttatggatggtggttaaagatatgagactcctgagttggagttgaa ggacagtttattagtgcagcaagcagtagcagtgaccagagtattagcttcctttttttt tttttgccgcttttccaagccctggttcccatatagtagtgtgaagaggagggctacatg atgcttgtacacactgtggattgcataacagatgcggaatcctcagcttcttacagaatc tgaatagtagtgtataataggaagtaagaatgcctttctttgctttggagcaagatactg tctttgtctcccaacactattgctgtaaaagtatcctcaaaaggatagtgcagaccaaaa gggtagttgagtgtcttgctcctaagaggtgcagaaacatgagagtctcatagaaaattg tctcccaataatattttctgctcatttctatactattttgtcttaaggcaaatttttttc atgagtataccactctgatttatctgacagaagatagtgaagtatctgttcaaatagtta gctggcttttaaaatcaggttatttgctttcttattgttgagtttgagagttctttcttg attctgggtacagtcctttgtcagatgtgtgatttgcaagtattttctctgagtctgtga cctgtcttttcatattcttcaatctcttttacagaacagaagtttctaattttgattact atcaggtttttctcttatgaattgtcttaggaactttttgcctagttgaaaatcacagag tttttttctttgttttgtccagatgttttattattttgtgtcttacatttagatctgtga tctgtcttgcattaccttttgtttgcataagttatgaggtagagttcaggttcattgttt ttgcgtatggatatttacttgttctagcactatttgttgaagagactatcttttccttcg gtgaactgcctttttacctttgtcaaaaatcaattgcccttatttgtaagtatattttgc tggatttattattctgttttgttgatttctgtgtgtgtcctcttgctaatacatcattgc cttaaatagtaacagttttatggtagttattgaaattagtgttctcaaattttgttcttt tgctgaattgttttggatattatagtacctttgcattttctttataaattaggaatcagc ttgttgatagctacaaagatactttgagattttgattggtgttatgtttaatgtacatgt cagtctgaggagactgacattttgccaatattgagccttccaatccatgaatatagttat ttttccattttttaggtctttgattattttcatcagtgctttatagttttcagcatgcag atcctgcacatattttgtggaagtgtatcttttccagatgctattgtgagtgctactgct ttaaaaatttttgatgtactaacttattgctgatatataaaaatatggtcgatttttaca tattgattttatattccacaacttgttaaaatcataatcataaaaccctttggattttct atatagacaacatgcttttgaataaaggctgttttacaaaatttcattttaaatttgtat accttttatttcatttcctttcttaattgcacttgctagagctttccaggacaatgctga atagaggtggtaaaaacacatctttgctttgttcctggtgttttattactcttttattat tagtgttatgtttagagatggggtcttgctcagttgcgaaggctggagtgcaccggtatg atcaaagctcactgcagcttcaaactgttgggcccaagtggtcttcctgccccgccttcc tgagtagctgggattacaggcatgtgccatcatgcctggctaagttttttattttttgtg gagatgaggtcttgctgtgttcacaggctggtcttgaactccagtctcaagtgatcctcc cacttgtactgggattacaggcattagccaccacacttggcctcttattatcattattat tattaatatatataaataaatatacatatatttatttattgagacagagtcttgctctgt cagccaggatggagtgcagtgccacaatctctgctcactcaacctctgcttcctgggttc aagtgattctcccacttcagcctcctgagtagctgggattactggcgtttgctaccatgc cgggcgtggtggctcacgcctgtaataccagcactttgggaggccaagacgggcggatca tgaggtcgggagatcgagaccatcctggctaacatggtgaaaccccgtctctactgaaaa tacaaaaattagctgggagtggtggcgtgcgcctgtagtcccagctacttgagaggctga ggcaggagaatcacttgaacctgggaggtggaggttgcagtgagccaagattatactatt gcactccagcctggcaacagtgagactccatcttaaaaaaatgttatgggatgaaatttt aaaaagacctttgaaagatacaagataataggattcaattctgtataaggtagagataaa ctgttgatttgtactagtttgatttgtgaaatattacaattaggctaaatcaaataggtg atgggtaatttgagaattgatttctaatgctttgtcctagatcattcaaaatctgatgtt cacataactacttagaactcttgactagttgtctgtgctttttaaaaacctttacctggc tggctacaacttttctaccgccataattaaaattgaaaacaaaataattagtctcttagg aaaataatttacgaacactatctaaacatgctagatttaaaactaccagtatttacatgg ttagaattcatgagcagtcttcattagacttactgctgaatgaccagcatacagtcgctg aattcattgtgcatcttttaagggaagattgttttgcttgtatctttgcagtcttctctg agatctttaatgttactagaagttataaagtttggttaattttcctaataagcctttatc tgattctgtgggctatttaggtttgggatgggatctgttgtcttaatttggtcaccattg gctcttggcttcaaagtggaccctaaactatattcctagtctgtctagtatttgtctttt ttttgctgagatatgtgacccctggggaggtttcccttattttcaataagttaatctttc tcttttaggaagaaaagacaaatttctcataagggagggattttttccttttttgttcta gagtgcctttcaaattaacaattttgttcatatcaaaagttgcccataatgatcactgta agtctcaagttattcttggtgaaaatatgccatttagaaagataagtatctgaattggaa gaagcatgtttcatgcttggagaggttgtggagttagactgacttaaaaagtcagttttg tgcagcctgggcaacataacaagacccagtttctatggaaaataaaaaaaaaattaggca ggcgtggtggtgcacacctgtagtcctagctactccagaagctgaggtggattgcttgag cccaggagtttgaggctgtggtgagctatggtaatgccactgcacttcagcctgggcaaa agagtaagacctcatcttttaaaatatatatacaggtttttcttcaaaagattccaaagg tactgctttaaaaaatgtaagattttctaagcaacagggccgagtactttgctttaaagt ttttatgaatgcctggcttggctatctggttgcttttgaaatcaacatcttactaaacta ttaatgcttttaaaatgtgtgcagaattacatatttgtaaatttaacttgagtctgtcta ggaaagcattagcaagacttttgtttttgtttttgtttttggagacagtcttgctctgtc gcccagcctggagtgcagtgcaggtcactgctcactgtagccttcacctcctgggctcat gctatcttcccacctcagcctcctgagtagctgggactacaggtgtacatcaccacaccc agctaagtaaaaagatgtatttttcatagagacggggtctccctatgttgccaggctggg cattagcaagattttacgggaaattttttttatcattagtagaaaataataaggaatccc ttgctggtaaaatttatattgaaactactcttaaacatatttatataaaatgaccagaga tatttaatttacctttatataataaatagaaataaattgaaatacattattaggagattc agattttttaattagtaaattagtgtgatggcttaaagttattgagaggtaatttttcat ctttgattagaaaaacttaaaaattgtataatttacatacacaaagtatatatgagcttt ttatattgatgaactttacaaaatgaacacacttttgtaaccacaacccagatcaagata tcagcttctcaggagcttccctgtatctgtcatattcctttacatgaaaggtaattaata ttctgactttgtaattagagattagcttggcttttttttgaatttttataaatcataaag tatataagactatatgatttataagttttttcaaactggcttctttcccacagcagtatg tttttgagatggatatttggcttacagcatttttcattttcactgaggcttagggtttca ttgtgtgaatatccttagtttatttctcccttctgctgatggacattcaaattatatcaa ttttgggttcttacaaatagtgctgctgtgataattctcgtacatatttttttgtgcata tatgtacacatttccattgagtgtatgctcaggggtagtatcacttagtctttgggttta tcttcagcttagtagacacttccgaacagtttcccatactgtttgtattatattattata catttttacctgcagtattttagaatgtcagttgctacagatttttgcttacacttggta ttgtctttttttagataaaagttagtcattttggagggtagtagtatgtctttgtgaagt tttgtttggtctttagcctatcttaaaaatcggtttgttttttttgttgagttgaagtat ttttaaagaatatattttgggtatgagtctttagtggaacatgtgcattgctgatatttt ctcctacactgtagcttgcattttcatttttaatttatttaaattttattttatttttta agagacagggtctcactctgtcacccaggctggagtgcagtggccttatcatagcttatt gcggccttgaactcttgggctcaagcagtcctcctgcttcagccttctgagtagctggga ctgcaggtatgtgccaccacacctgggtaatttttaaatttttttgtagagacagaatct cactatgttgcccacactaatctcggactcctggcctcaagcagtcctcctgcctgggtc tcccaaaatgctgggattacaggtgtgagccactgcgccagtcatttccttctttccttc tttctctctttccctctttccctctctctttctctcttcctttctctctccttccttcct tccatccttccctccctccctccctccccattcccttccccttccctccccctttccctc cctgcttccctccttccttccctccttccttctctctttccttccctccttccttccctt tttccttccttccttccctccttccctccttccttctctccttccttccctccttccttc cctccctgtaattgtaatgaaattgtttgtttgtaatgaaattcagtttttttatgtgct gtgtattaaatctatgcctaaatatcgtgacagtatttatcttgtcactatataagcttt attatttaaaattttatatatataggtctctaatatatcttattttttttcccacctttt ctgtggcttgtggatagatgttattcattttactgattttgattttaacagtagtagtca tagaaaatctattgatgtggcttactcatgagaaagcatttttatttttatatgtgatat tataatttctagtatggcaacattttctgctagttatgatatgtgatttttactctgcag attctttaagaggaatttaaaaacagctttattcctgactttagagaaggcagtcagttt tacttaatatgtcattttatgtcttatttgttatgttttgtttttgaggagtcatactgg tagtttaaaaatactgagttttatcatcaaatgcagctacaatgtttaagcagatgactt aatctccgtgaggcatgaggtgattaaaaaatgtatgtatatgctttttcaaatacagag taaattgcctggcagacaagaagagcttcataaatattatttagtgctttttattctaga tttttgaaataaattggactcgacttgatgcaaaggatttgtactataagtttattataa aattattaaccgctagcttttgtaaatagtaaaattgatttgcccattatacatttcttt ttgttatgcataaaatattttaatttttaagtaccaacataggatggatttttaaatgct tcctcatgttaattttttaataaacatagaatgaatactgtgtgaaatattttcccactt tattggctctgttttaactcagtagactctatagttagattgtctaggtgccacttaaaa gatggtaactagataccagaatgtgttacattatatgttgattgtcaggaagaactgaat gcatgaatgcttccctaagacattgttagcatgccgtggtcttgatctataattttttgt ctaagttgtgatagagctcattctttacatggtctagtaaattcagccaatgtctttata attcattagcatctctatatattacctataaaatttaaattataaggctatgaaaaaatt gtttcatatgtataatataccaaagttttagcatatggtttggattttaaactgttttac tgaaacaaattaccgtcattaaatggaattcttaggtagacattacagagtaggtgtaac tctgaagttgaaaatggtttcatcttcttttttaaaaaattttattatatagggaatctc aaccaccagcaggattttaaaaaggctatctaaatatttggtaagtaaatattctccatg ttaaatattttgcgtgttacttaagtttacacatatgctacatgaatgacatttacaata gtgagtgtaaattatagttataattaatattttaaattttacttggttactagaattttt gtctccttaaacctattccaatccttttctctcttgttctttatgttgtgtccagagatt ctttcagtgtacagccttattattaagagttcaaggaaagaaatggttttagtgagcagg aatgctaatactttatgaataaaacaggatttcccctacttgagattttgtagtatttct tttttgtgaaaacactaatacagatttgttctttagccatccattgcttgactcctcctt gtatttaggtcctgtttgctgcaacatgccaacatgaaccaaatactgtggtcagggtct atgcatttcctgtttagattattctttgttccaagcttaaaatatgaaaaattttgtagg tgtctgtagttaagaatttcataaacttatgatttcatagataaagcttttactttttta tttttgagtaaaggtaggtgagtcaatctcagaatgtattttacttcaactacatagtat tttatacctgtgtactatggtgattatatagatataaaatgatttggttaatcataatag attttaaagttattaaatatgcttcagaatgggaaagaagtgctgagacattgcataatg aggtgtcatagtataggcttttggttgtttttaatatagtcaatagcagattattaagga ggaaatatttagtgataagcattggcttcttttattatactttttaatgtgtttgaaaca gcaaatatttgaccaaattatttttcttaaattttatagtaactacagtaggtgcctaaa tgttcaacagttatactgttctgtttactgtaaatttgtaataaatataaatacttttag gagcaaatgatgccacagagatctcttaaacagatttttactactttagaaggtggtaac tgatttctggagttgtattaagtacaaaagagtaacttattgtcatttttttccctattg atgcgtatatttgagttagagggaagcagtcgtctttgtgttatatgcttatcatgtata tttcatcttagcaaatttattaattttagtgacagttttattgagatgaaatttatatac caggagatttcatctctaagtgtacaattcagtgatattttaatatattcaccaagttat gcaactaccagtactttgtaatcttaggttactttcatcatccttcttctggacgccctc tatcacccagccctaggcaaccactaatacactttctgtttgtataggtttgcctattct gggcattttataaaaacagaatcataaaatatttgtccttttgtgtctggcttctttaat ttagcatgatatttaaagcttcactcatgttgtagcatgtatcagtgtttcattcatttt tgtggctgaatatttctttgtatatcatttgtgtattcatcagttaatgagttgtatcca cttctttttttggctattaggagcgcttttctgtgaacattcttatataagttttgtgta gatacgtttttgagtatatatggatggggtgggattgctcgataatatagtacctgtgtg taactctttgaggaactctcaaaatggctgtaccattttacaataccaccaacaatgtat gaggattgtaatttctccacgacctcataaacacttattgtcagtcttttgtatttcagc cattctagtaggtatgaagttgtatcccattgtgattttgatttggataatgactaatga taagcatcatttcacatgcttattgaccatatatcttctttggagaaatatctgttcaac tcctttgcccattaaaaaaaaaaagattatctttttaatgttgaatattgtaaacattct ttatatattctggaaactagtcccttatcacatacatgatttgcaaatattttctcctat ggattatctctttacttttatgatggtgtcctttgaagcagaaaagttttaattttgaag ttcagctgatctgttttttgttgcttgttcttttggtgttatattgcagatttgttttta aataaatactcactgatagcctgggtctatgacaaagttttaatcgtcataggactacga aaaagcattggcatttcttgtgtttgacagtcaaagcccaaaggaggcaattatccttgt atttgtgcttcagttcctcagctaattgttttaagcaaaaccttacgttttgatcccatg tgttactgtgaaaatattagctgtgtgtgatatgtttattgatattagtcttgtgaattt atgtttagttttttttggattgaagaagggaggggccatccttaatagatgttattagtc cgttctcacgctgctgataaagacatatacccaagactgggtagtttataaaggaaagag gcttaattgactcacagttcctcagggctggagaggcctcaggaatcttacagttgtggc agaagaggaaccaaacacatggtgacagcaaggagaagtgcagagtgaatcgggggaaaa gccccttataaaaccatcagatcttgtgggaactcagtcactattatgagaacagcatgg aggtcatcatccccatgattcgattaccttgcactgggtcccaccacaacacttggggat tgtgagaactgcaattcaagatgagatttgggtttggacacagccaaaccatatcaatag tttattatgacaattactgcatattttatttcatagtttaagaaaccattgctttttaga ctaaggattatcaggttcttatctttcttgtgaagaaggaatagattttgataatctcac tgattttaatcttgtacagattattattattattattttgagacggaatctcgctctttc gctcaggccagagtgcagtggcgcgatctcagctcactgccagctctgcctcctgggttc acgccattctcctgcctcagcctcccgagtagctgggactacaggtgcccgccaccgcgc ctggctaattttttgtatttttagtagagatggcatgtcaccatgttagccaggatggtc tcgatctcctgacctcgtgatccgcccacctcggcctcccaaagtgctgggattacaggt gtgagccaccacgcccaacccagattatttttaagcaatgatattttggtgtttttgtaa acttatgtatggaatatgtaagtttttcctctattaagtaaatgtctttaattttttttt ttttttgaaatggagtctcgctctgttgcccaggctggagggcagtggcacaatcttggc tcactgcaacctctgcctcccgggttcaagtgattctcatgcctgagcctcccgagtagc tgcgattacaggcatgtgccaccaaacctgactaatttttgtatttttagaagagacggg gtttcaccatgttggtcaggctggtctcaaactcctgacctcaggtgaccacctgcctct gcctcccaaagtgctgggattacaggcttgagccactgcgcccagccagtgtctttaatt tttaaggtaccaatttagttccttattttttatcgttatgtgacgttatgtgatcagttg aaggtgtatttcatgtattttgacctataatgttgtctagtttagtttctggagaaatgg tgtgtaacagtggaatgtcacttgtcttcaaagcattagctttctgtgtaaggttgaggt tgtaatataaatgcgctagatgtgtttttattaggggaattgactattcttacctttcat gttatttgacagtattctttgtcaacttcaaataacggcctattttgatgagattttata gtgctaggatcactgatattcttgttagttgatttaaaatcatggtgttcattggctaac agtcacttgagtggctattagatgggtaatattgttctcttttttttttttttttttgag aaagagtctcactctgtcgcccaggctggagtgcagtggcatgatcttggctcactgcaa cctctgcctcctgggttcaagcgattctcctgtgtcagcctcctgagtagctgggattat aggcgcatgccatcatgcccggctaatttttgtatttttagtagagatggggtctcacca tgttggacaggctggtctctaactcctgacctcaggtatccacccactttggcctcccaa agtgctgggattgcaggcatgagccaccgcgcctggccagatgggtaatattgttctata cattgatattcttatggtttatgttatagtattcatggaaatttagcaatggaaaaggaa atgaaaaacttattaatgatgttgttaaattttcaattgtgatgacagtaaggaaaagac ataattgagagctacctccaattgtttatatcaaatgtggtaattaagaggattttggtg acctatttatggctgaattaaggcaaaatagcttttatatccgattctttcctcttccct tctcttgttctgtgtgcttactatcataataattgagtatacattaattttctttaagta tttatattttaaataaggttttgatgtcagtagagacttttttttttccttgaaggtaag taccctttaaaccactaatttgtcactagagtctatagtcgcttccatatataataggta gcacagcttctcaggtcatgtcctgctttgctgctctgctgaccaataatacaccttgga gatactggttctggttatatgaatagaacaattaaattgttcttcattagatggatcttt gattactaatcttactcagaaaggctcaactggacttggattattctaattgccaatact gtttttgcacaaatgtctgcaaatcattgaacagtaatactgacccctttttcttccaat aaagttattgcagtaatcacatcattgcagagaatatttcttaaaagcttttttcaatat ttacttctttaatgaaaaacttaatccagattaagccacatacatagacgtcttgacttt attattttgaatacctgagatttccaaaatctctttaactgtcttcacacaatttctagt ctgtcattttaattaattacaactgttcatttcttctgcttttgtttcctcttcatgtgc tgcctagcgttgctagagtttcatcagtttcctgccttgagaggtttctagtacaaatta acattttcccttcctagtttatgcaggttccttcctggtaatttttatctttttgatctg tttcattctgcatgttagttatttggaaccattctactgtgctcaaatgaacagtcctat ccctagttctcttattcctttttcatttcatattttataccctttttcctcagtctgtct taaagaataaagtgttttcatttcaaaagcttattatctctgtttttaatcttagccctt ctttcctctactatttttcttcctcttaatgctccatgctgggttctctgccttatcaga cttaatcttgaaaagtacgttacttccttcccattatctccaaggttgttatagtccatt ctaggtgtctccaatttctcaactcttactcaattactttctgaagtttgactattattt ctactagtgttcagctttcttaagtgtaagtaaacacatttcttacttaactaggtagat aaacattagctggacggatctggccgtaaacatgctattatttccaagataaaactgagt tccaaatggctaaatctttgtcttttcacttttcatccattcctgtcttttttagcattt gtttttttgctgcttaacattcacttctcctttgtcttctgtaataaaccttaatggttt ttctcctctctctggctaaatgtttgttttctttgttactgctttcttcttttatatacc acttatatataaattgggttgaagctttgctcttagctctttttcattacacatcatttt gaggagatggtctcgtgttcattatgtagattatcacaaatttgatgactcagaaatacc acatttttcttagtgacctgttttaccactttctcagtcgccaggtccttagcaccccaa aattgttatttcccagtggaaagtttgtcttgtacaatgcttgctatatggttggaacta agtaaatgctttttgaatcatgaactaactccttatctgtctttttttttcttaagataa tggttttaactgtccacctattctcatgtcacaagccctgtaatatctctgaccacttct tgtctacacttgttaatattagttgtcatattttggtcctaagtttgactaatgaggaag atgacatttttgtctgcagatggtggaaataaaaatcacagagattgtgatttccttatg gttttgtgggtaatggtggagtttaaacttacaacgaagtttctggtgacatgtttccta gttttcacagaaaacattctttttttttttgagacagaaagtctcactgtgtcgcccagg ctggagtgcagtggtgtgatgtgtcggctcactgcaacctctgcctcctggattcaagtg attctcatgcgtcagcctccaagtagctgggattacaggcgcccaccaccacgcccagct aatttttgtatttttagtatagatggagtttcaccatgttgaccaggctggtcttgaact cctggcctcaagtgacccacctgtctcagcctcccaaagtgctgggattacaggcataag ccaccacacccagccaaaaacattctttataatgatacaagtaatataccagacaagaaa ttactcagtgtactatgtgagattaaaaaaaaaaaaaaaaaaagactggaagtggaagtg ggagtacttcaaattcttgtctgatgaggtaaagattttaacttggtggaaggaatagaa atcaaggatgtgagaaatttttcattaattagaatttcgagattcttcttttaagaatgc agtgatgctagtgaagtggttgagtgaaccatctttcagctaacaattataagatctgga caaaatattgaaagaaaaactttaactgttttaaaagccatggaaagtaaccaaaaaagc aaagccagatgagagcctccaactgcaatgtgtaattgtatgtttgtggctttcttgcct tatggtgcattttcgcctcctgtctttcagtggcataggaaagaagttacgggtagcctg gcagttggaaatttactcaggggaatcatggaagcaataagtaagatccaaaatctgagt gtaaactctgtccaaatcatggctaatcacgaaactatacatgcataggggagaccccag gggacccagtgtaaaaacaggcagaaacaagagcggaatctgctcctgaaagaattgaac cctgtgagatttgtgattgcttctttttttgacagtacatttctcaacttgcatgcaatt tgcgtggccaacagctggcttgaggtatcagagcacagaacatattgttgacagaagagt taaaatttagggaacaaacactgaaagcaagataacatcagaggatgtaggccacacatc tcagtataatctctgcccatttccttcactgaccaccagactacccaggtgcagagggga tgccctgggcatcaggttagaagaaggaagcagctggaaggtgaaagaactaagcaaaat cattgttgcttactataagggagacacacagtaagtcctcacttaacattattgataggt tcttggaatctgactttaactgaaacaacgtataacagaacatttttttctcatcagttg tataacaaagcagtgttaaaggaaacgacagtattcaaggaccttgctgtttgtgttttg cttcaagttgctgtttccaagacctatcgatggtattaagtgaggacttactgaagtttg cagtttgagtccatacgagcccattagaatcatacaacatgaaaaccaaatggtcctttg aaagattgcgacaaaatctggttgctataacttgctatttacaatgtgtattttcaacta aaaattattagatatgcaaataaagtggaaatatgactcattttcaggtaaagaaaatca ttcaatagaaattaaccccaagtatagccagatattggatttagcaagcaaggactttaa aatagccctaaatataatcagaagaatttaaggagtcctatgttcaaaggattaaagaaa aatacagtcacccctctgtttccatgtttctgcatttgcagattcaaccaaccgtggatc aaaaatagttgggaaaaaacataataaaatttacagtacagcaataaaaaataatgccaa taaaaaacaataaagtcttgttcagtgaatagataggagatcaaaacagagaaacagata ctgtaaagaagaagcaaaagagaattttagggttgagatcagtaactggaatgaaatttt tatattatattttattttattttattttgtggtggagtcttgctccgtcgcccaggctgg agtgcagtggtgcgatctcggctcaccacaacctccgcctcctgagtagctgggattaca gacatgcaccaacatgtctggctaatttttgtatttttagtagagacagggtctcaccat gttggccaggctggtcttgaactccctcccgacctcaggtgatctgcctaccttggcctc ccaaagtactttgattacaggcgtgagccactgtgctcggccaagcaaaacagttgtttg gagatggcaaaagaatcagttaaagatagattaataaaagtcatctatttgaaagagaaa gaatggagaaaaataagcagagtttgagagacctttggggtaatacaatgtttgaacatg catctaaatggagtctcagatggggaataaataatggctgaaaacccccacaactttggt ggaaagtatcaatttacatatccaggaagctcagcaaacttcaagcaggataaacacaca caaaaaaacagatgttagcacataagtggtcaaattgctcaataccagagaatttcttga aagtagccagaaatatcttaccagaaacaatggcgacctctagccatggatatggtatat tcagctgaaaggcaaaaagctatcaaataagaattctgtgttcagctagaagtgaaggta aaataaatacatgttcagataaaccaaaactgggaaattttcaccagccagccaccattg caagcatttctaaaggaatcaatttcaagggaaatggcaccagatggcaagtaccatcta caggaggaaatcaacatcaaaactagtaaatacataatttactctaatttttataaacta tatttttccttattttaaaaacaagtacctgtgacttgaagcaaaaattatgatgctgta ttgttgggtttataatggagtttgggaatttacatattgtacaatttttaatatgtaatg acatacaaattaacgtgttaatagtcttctaacttatgaacatggtatatctcattaagg ttattgtttaattttagtaatgtataataggtttctaaaaaacttaaaaatgtggtaaaa tatgtacaaaataaaatttgccattttagctgtacaatttggtggcacagttgcattcac agtgttgttcgttcattactactttttccagaacttttctatcattccaaacagaaactc tgtacctactaagcagtaatttcccatttcccttccccgagtctctggcagtgtctaatc tactttctgtctttatgaatttggctattctgtatttcatatatagtagtcccccttatc cacagttttgctttccacaattttggttatctgtggtcatccatggtctgaaaataggtg attatagcacaataagatgttttgagagaccacattcatataactttattacagtataat tgttcttttttattattgttgttgttaatcttttactttgcccaatttacagattaaact aataagtatatatgtataggaaaagacatagtatatatagagtttggttctatctgtggt ttcaagcatccactggggggtctggaacatatgctctgtggataagtgggggactagtgt acttcctgccctaccccttggtttggagtcgctgctgtatgaatataagggcagtctgcc tgtgatgtgactctccattgatcacctgggtgtatgaattaattgctgtctataaagagt cttggaatgactattgagtttgctctgtgtatgtatacatacaacagtatggctactgtt gtagctgatttggctatgatactgtagtatcttatttgctccaaagagatggagtagtga tgaaatctggtttactaggtggaagctagtgtaaaaatgggtactctgttttattgaatt agcttgattggaaaaagtgaagtgctgatggttttgaaaatatgatgataatgatgtcat ccttctggtttaaatattttgtagcacttgtggtagattgaatgctggtgtcggtagtaa agtcatgctgcagttatagtctgaaccagctgtactgttttgggtagtaacttagacagt agagaacaccacttttctaggcagggctcctcacctctcctagggggccatttcactgca tcttggagtgaatatacagagaggaagtagctagatcctaatttctacaagttatatcag ttggggaacagttgtggttgtcaacctgtgttagggctgtattggttttgctttcagttg tgctataaagtggaaaaaattgaattgtttatttatctcttattgtgaaactttcctgtg gcccatagaagtggcatagcaagctgaacatagctgggtttgttcttcattgcaagatcc tacccccacctccctttctttcctgcctggtttcttgtgtccatactgcaagctcttttt cccagttagaagtaagttagttcactcttaatagagctgatttacaaaggaattttatgt gtgtcatactgtttgattctctgaaaggtagatatgggactgtggtaacaatccagcatg atgttaactgtgctctcatttttaaatgtgcaaatggatgtatgtgaaatggtggtttgt ctgatacaatattggctgctaagacatgtctgacctagagcctagatgtgttggcttctt aataatgctggatgatgtatatctggatcattctttttaattgactgagatgatgcgtat cttctacctcttgagagttaggaatgtaaagggcaagtgaaaaatttgttagatgctctt aaaagatgaaaatagatgtgctccatcactagtatagtttctttttgaccactatgtcaa cctgtttagaaagtatattttaggccgggcgcggtggctcacgcctgtaatcccagcact ttggggaggctgaggcgggcggatcacgaggtcgggagttcaagaccagcctggccaaca tggtgaaaccccatctctactaaaaatacaaaaattagctgcgcgtggtggtgcgtgcct gtaatcccagctacttgggaggctgaggcaggagaatctcttgaacccgggaggcagagg ttgcagtgagctgagactgcaccagtgcactggcatggcaacagagactgccaacagagc gagactccatctcaaaaacaaaacaaaacaaaacaaaaaacaaagaaagtatattgtaaa catcaaattaaaagaatttcctgaagtaactttttttttttttagtaggggtggggaaga tatgagtagaagagaaatgaattggaaactgagtaccactaccctttttcttaccttcta tccagaaatctttcttaaattccacagtatcagtgtaacatcttttatcttttgagctgt ctttgagattctttactttgtctccagactgtttgttaggattctactagagaagttgtt ttcagattttaatttctctgctccattttaatcttctaattttttaatgaaaaaaaatcc agttttattgagttcttttttttttttttttttttttgagacagagtcttgctctgtcac ccaggctggagtgcagtggcacgatctcagctcactgcaagctccgcctcctgggttcac gccattctcctgcctcagcctcccaagtagctgggactacaggcgcctgccaccacgccc agctaattttttttgtatttttagcagagatggggtttcaccgtgttagccaggatggtc tcgctctcctgacctcgtgatccgcctgcctcggcctcccagagtgctgggattacaggc gtgagccactgcacccggcctattgagttctaattaatgcataatttgttgagttttgac gtatatgtgcactcatgaaaccatcaccacaatgtatatatccagtgaatatatacatca cccttaaaagcctctttgtaatctcttcactccccacttcgcaggcaaccactgatttgc tttctgttactgtaggtcagtttgtatattcttgaatgttatgtaggtagaatcatataa tgtgtctcctcttttgtctgacttctttcaaccctagctcaatatatttgagatttgtct atattgttggatgaatgatcaaaactttgtttttgtttttttaactggggaatagtattt catttttagtatatagtacaatttattttttgcccatttccatttagctgttattgctgg attgggttattaacagttattgactattatgaataaagtggtcacaaatgttggtgcatg gttttttatgtggacttgtgctcttatttctgctatgagtggaatggctgattcatatag taggtgtatgtttacctgtataagacactgccaaacttttccggtttgtatcagtttata ttaccacttgcagtgtatgagcattccagttgcttcacatcctcagaattcttggtatgg acagtattttaatttgatttgttttagtagctgtataataatgtcttattgtggcattca ttaatcattaggaaaatgcgaattatgttgaacatctttacctatgcttgtttgccattc acatctttttctgttgaagtattttctcgaatctttgcttattgatttattgatttattt attttgagtaagagtcttgctctgtctcctaggctggagtgcagtggtgtgatcatagct cactgcagccttgaactcctgagttcaagtgatccccctgcctcagcctcctgagtagct gggactacagatgtgtactacaacacttggctgttttttttttttttttttttttttttt aaggatttttttaaagagatagggcctgactgttgcccaggctggtctcaaacttctggc ctcaagccatccttccacttcagcctccagaagtgcagggattacaggcttgagccacag tgcctaaattttttcattgtattgtttgtcttattattgaattataagagttctttctat gtgctaaatactagtcctttggataattacttgcagatttttttcagtttgtggtttgcc tttttttctttacccatgtcttctcaagttcaagtcttttattttgatgaatacacattt atagtttttcttttatatttcatgctttttgtgttgtattttggcaggccaaataatgac ccctgaaagatgtccatatcctaatttccaaaagctgtatatatgttacctgatatggct ccacaaactttgcatatgtgattaagttaaagattttgaaatgaggagattagccaggat tgtccaggtgagcccagtgtaatcagagagtccttgtaagaaggaggcaaaaccaatcag ggcagaagagatgaatgactgcagcagaggttggaatgatgtgctttgaagatgaaggga aggggccatgagctaaggccatgagataataaatttgtgttgctttacaccaagtttata gtaatttcttaccacagcccatatagattttgcctgtaagtggaatgccgctgtaacaaa tacctaaaaaggtgaaagtggctttgaaattggggtattggcagaagctggtagaatttt gaggaacataatggagaaaacctagattatcttgaatgtacagatggtagaaatacagat gttaaaggctctgctggtgatgccccagaaggaagtgaggagcacagtagagaaaatgtg tatcgtcttagagaatacctaaatcatcataaagagactgttggtggaaatgtgaacatt aaaggtgctgctgcttgtgagtgctttgaaggaaatgaggaagatgttattggaaactga agtgaaggagatccttgtttagattgtaacagaaagtttacctgaattccgtcctgtagt tatgtgactaactctcctgtgttgcctgggtatagcagcaatcatttatacatacatttg cagttctgttctgtacttgttataaagtagttttaattaaagtgacagatttatgttttt ataaattaaatttatatttaaaaaatcagtatttagaacaaatatcttagtggactatat tcttgagatattattattaaagtctttttacagcttcctttcaaataaatggatatgtaa tttaaacctttaatttctggatttcctgatgacttttctttaaaaagaataatttttaaa aacacattttaatttataactaaaatggttatttaaggaaacacatatattagttttatt tagatatatgcttttttattattaatttggaaagtggaggtgcattgtttggtagaataa ttttaaatggtagctcttgttaatggctaatatttattagtggatacaacttacaaatat ctgtacattcagtctttatccttatcttccatatctttttcttctgcattctaggttttt ttgtttgttttttggaaggaggatatataaagtaattattactacatttttagctgttgc caagttgaaagtcagtgtttagttttttttaaaaagggctatttagtgtggaattcctaa atattatagttccctcttctgtcctttcagagctattgcaccctgttaaattttttttgc attcagttcaaccagtgtttgaaaattaccaatagacttaaagtacttctctgaagttag aagagctcctatgtctgatgctatattgctagtatttctattcaggttggaatttggaat agtcattatcaaaagttatttgtaggcaaaagaaaacaaaaagaaaaaagttatctgtag gaaatattggagactggggtacaaggtaagtgacacagtgaagaagcacagacaatttca gaatatgagacacctaacatggtttctgcaaggtattggcgtggaaaaaaaaaaaaaagg gggggggggcttagagagttactctccagtaaaagagatataagaagcagtactatagtt atatacaaagggtagttctttggattctgtttagatcaaattaactagaaaatacatttt taaaataagatggggaatttgaatattgactggtattacttaatatcgtggaaatatact tattttgaatgtgtatagtgactttgtgagtatgaaaggcagtggccaaattatttagtg atacataatgatgtatatggatgagggaagtaatgacgtgatgtctggaatttactttaa aataccttggaaaaaatgaataaaagatagataaagcaatattgctaatatttcagtacc tgttaaatataggtgacagtatatatatagaggttcattatactcctttctccacgtttt gaaaaatttcatttaaaaattcaaaaaaattctaaaaaatattttttcttttagaaacta acacatgctgaaaaaattcaaattttgttcactgaccacgtttttgctgccaccactctt tcaacatttaaaaaatgttcttaaatctttaaaaattcgatttacattttcattaagaaa atgaggcatttgctggccatttcatccttcctcttgtattttttctccttacctgctgcc accgtttactagaaaaatatttctcttgttttttctgtaccttctccttgaaatataaaa gttcagtgaaggcaggagagattttagcccgtttttgtatggctgcatcttccttatcca tacctgcatagcatgtggtaggtgctgagtaaatatttgaatgactgaatgggtgactga atagactgaatgaaccagagttgtagaagacatggggtgacgtggggtttggctaaggac agaaccaaggagaacccctttgaaaattcactctgtgggatttacaggggaaaatgtggg ctactacataagaaattaagataagaaatcatctttcctgtttgactgtaaagccttaat atttcaagcatttaacatattctcagcataaataattaagtttttttcttcttttttctg aaacagtctcactctgttgcccaggctggagtgcaatggtgcgatcttggctcactgcaa cctccgcctcccaggttcaagtgattctcctgcctcagcctcccgagtagctgggattac aggcatgtgccaccacgcctggctaattttttgtatttttagttgagacgggggtttcac catgttggccaggctggtctcaaactcctgacctcaggaacccacctgcctcagcctccc agagtggtgggattacaggcgtgagccactgcgcctggccagtaattaagtttttatact gtaagttctctaattgtctttaatgtattcttgaaagtgttttcctcctaatttatttat tactgcaaggcaatccgttcttgaggttctatttaaagtttatgtatttcttctcaggaa aaaaaattacttaaaactagtggtttgtacagtcaagatgagttttagaagctgttctta taatttctttttatgttaaacatatataatgtcacatttcccttttcctaacaactgatc ttctttctttttcaacgtgtatttataagctagatttttaaattttgctccagggactgc tgtagttgttctactacttaaaaaaagaaagatgttagtattgaacactattctgagaca cagctgataacaattgtgctcaacaatgaagatggctaaaaattgggttttaaaaataac agtgaaaattcagaagcattttatacttgctattctaaagtgagtatttttctaatctct gctttaaaattactgaagtccttttaatgaccaacgctgtattttaaggaaaaaatgtga gcaaagatttttagtgattctaaatttgttttgctctctggatctctttaaacttttaaa aattattgaagaccccaaaaagctttgacttatgtgggttatctctcttgatatttaccc tgttagaaaataaagctgagaagtttaaatagttttcatttattaaatttatttaaaaat aacagctaattacatgtttacacaattaacaaagtctggcttacaggaagacagttggat gctcatatctgcttttgcattcagtttgttaggatattacatgctctatactctgacaaa aactttactttgtgtatactcaagagacagagtaaaaaacataagtaacatcttagtatt atagaaataattttgatcttagaaactccatgtaagtgtctcaggaactcccagggttca tattttgaaaattgctggagtcatctctcagaggctatgtgtgacaaatggtgtttttaa atgaaacaataatttttaaaatatagtgtcaattggctaagtttttatttgatacttttt ttttcttttttttttttgagacagggtcttgctctgtcgtccaagctggagtgcggtcac agctcactgcagcctccacctcctgggctcaagtgatcttcccactttagcttcccaagt aggaagtaggtgggactacaggcaagtgccaccatgcctcagtaatttttgtatgttttg tggagacagggtttaccatgttgcccaggctgctctcatactcctgggctcaagcagtct acttgcacccaactcagcatcccagagttctgggagccggattcttatttgatacatttt tacctttaggggaactataaaaattagcttatgcttatttatgcaaataaatttgagcat tgaggaaggatgtctcttgttcagggttgtgtttgtagatttcacatcttttagactacc atttaagagaagtgatcaccttgtatcttaaggaaatgaaaatcatttgatagaaatgga ttgttaaatatttttaaaacaagttttgacagttacttgcaattaaaggggaaagcaact ttaaaatgcattagttaattgaagaaatactcaagatccttaattgttgttagtgagcaa gtattaatgttctttcctctttgaaaacgtatctgtacctagacaaagttaattataaac tttttcataaacaatattatgcttctttttcttctaatttttaaaccttatctttgttgg tttgccttccagctccatctatctgcaacctaagaatcaacaatactatacatcaaatta gctgatgtggaaggaaaatgataaataatgaaaatacgtcatattgcttatactctaagt tataattaatataagatccaagggatattggtgtgggggacgaggaggaaggaacattgc aagtcaagctatttcacaagtattctatacctttgaatcttttatttcatagttttcttt agaaacaatagtgatgcagactgaatagctagtaagataactttcattaatagcagtatg gcagggatttttaattgatgcttgttggtaatattagacacatgatttatttataggctt ttaattagaaaactaatacaaatatcaaattgttatggacaggttatacattttttggca acacaaatcctaccaggttttaatgcctggtacttgtttttacattagtatgtggtatta attttttcattactactcttagcttccctagcttacccatttgctaggagtacatagcag cactaggacatttttcgctaacgtggctggcatgcatctgaaagtgtgacagagcagggg tatagctagattttagaagttcttttgctcatttaagaaacttaaacttcagatttgata gtacttcagattctacataatgtgctctttcgatgaatagctgtgtagcagcagcacagg aaaagcagagttcttggcaatacccagttcaattgcatctgcagatgaggatttaaaatt ctgtgcagcagacagtacctccagcaggcgagtaaaaggttactgcattatttttcttgt ttgttaccatagcctttatctaccaaaggatttttaaaaaattgaatgtgaagtgcatgc ctgtttttagatattctggaaattgatttaagtataacatgaatatcaaaagggcagtgc aaggtgaaggcagaggcagtgctggatctttttagctttaaaccagggaagaaattgagc atgttacatacacaaagaagaaacctttcagtgtcacagacaatgggaagttttaaaata cgtattttaattcaaggtgtttattatttagttttagactatcttccccaaaaagggtgc tcttacattagcaatcattggtctatgaatgttcatttaagttacagtaatttgaagtat gattatttgtgaagactataaagtatttgtattaatgcgaccctcccttgtcctggaaat tgtaatgtggattaattacattgtataaacaaatacctttgtgcattattggcaaaggta gaatgcttaagtataaaaggtatagtatagctagcatacttaatattgttgcttatgttt tcagggtggtttgtggtttttaaaataatcttttaaataaaatgcagacaagtaagtggg agaaaattcagtatcaaaacaatgaggtttttgatggctttttacttgtggtcagtacct acctatatagccatatgtccttttttctcattaaaactcttctaacatttatgatagcag gagcctccttggattatccagcttgtttattacatcattatctgcaccagtatttttgct atagatttttattgttcattacttctcaagtctacataaagtatccagagggaatttttg cttgtatattttattcagttaccctcctaccctcttccatcagtcctcaaagatttgtat tagctactgtctacattttgtttatttaaaaaatacttaggcctatatttttgtattgta gattgaataataagattgaaaacaattctgtaaattaatctatagtatagaagtaaaatt tgttgattattcttactgtgagtatttggcaggcttcttgaattgtagagtcataaaagg tcagcagttctgattggtaagttaaagaatccatccagtaatttgctccctgttgtttct caaagcaatgcagcagcagttcttttccagatattaaacggttcttgaacagtgtgtgtg gagacggggaggggtggagctcattcctgttggttgttgaaatcgtgtgcatttacagag ggaacaggttggataggctgagctgaaggagtaggcaaacagatctaaactgatggaaga cagaaagtggttagtataattttggggtgtcttgagtttgctcctttgttaagtacttgg gctttaatataaaacaatttaaaatattaaagcaccttaccttctttctaaagagtagct gggagacttctgttttaatatccagtttttcttggcgatgagtttgtggctggcatcttc tgtagcaaagctgttgttttattcttttttagtagtgtttctttcagccagagagctgca gaatgaggcagtttgacaattttgagtatatatgtgacatcaccagttctgattttttaa gtcaaattctcatctaatgagcaagtcaatgtgtggctgtggctgcagcattatttcttc tttaatgaagaggttgagccgttcgacctgctgctccgatttgttctcttgtagccatac atcagaccagttgcagagattatccatcgaatgtcccaggcaaccaaaggtaactacata gatttatttcaaataaaaatatgcaatgaaaatggatgcattatgactaagaccaaatga ttaaaaataaaagaccaattaaagatgttcttagcagttttcttgaccttgcagtagata tccaatatcattttgtcatcatcagattgtgtagcaatggaaatgcactgcagtaattga ttttgtaataggatcaggtgatttactagctgcactgacaaccacttgcttgcttgctct gagctgtggagcactctaatggatgttgtgatttcagcctggagtttatgtgagactgcc ggccacttaaagcagcagcacttattttaaagattagattagtttttctttcttgttttc ttcgtttcaagttttgtgagtagcctcagtaactttatggttaagttgtatgccttcatg aaattttagagattatatattatttcattcatcaattcatagtctttctgctccatattc ctagctaaatatcaacatattctggtattgaccatgagagcatatctctaaaatatgaga gttattggtaactatgctgtgttatctaaatgaagtggaatattcctcacattcgtagat ttcattagcttcagactctagctgtaattagaatgatggtaaggttcttgatctcttgag ttgtactgcagttgttttgcatccttttttggtcttttactttcttagtggtttcatggg taaggcaccatttaggaaatatgagttgtattacttctaagggatactgatgaggatata taaagctattttaaagtagtgtttaaggatatagcaaattaaaaatctaatatcaagtat tataaatttcaaagtgattattttaaaataatttttgttttccttttctatgccttttaa acaaataattggttcaaatatagaagtgtaggaatattgctaactgtaaaatagaactac tgtcatagaaactcagatgctgtcaaagactttgattacttaaaagttttgctgatggtg ttagattagaaaagaaactctcttccactcccttcctcaccaatacctctacctcatgta aagtgttttatacagactccaccacataaaaatactgaattctttatcattccctgtttc tgttcttgccatgatagagacaccatttctctcaaccacatctaaaaacatttacaaaaa ttaaataagattaacaatttactgtagtagaaacggggcataaaattgtcatcacatgtg gtattcaaatcaccatgttaagagaactttcctttttgatgtactcaaatagtcacttgt agtgtttgaagccttagtgtttctagaaagttgaaaatattatctgtgctagtctgcaca tttcctttaattcagatactttaaacattaattatggaaaattgaaaataatttaaacac tagtatttgtaatcttttattattcaacaggtaaaaagtttatagactctcttgacttcc aagaaaaaaacccttctgtgaacactgatgaacataagcatgttaatatcatttaggatt cggtcaaggatgtgtctgaatttcatatatattgaaaatgtttaatgatgggccaccagc aaattaatcatggatatgttttactggagtgccgtctatgacagcttcttttcatgatgg gttcagcaaataggaaacgaggaagtaaacaccagagtgttgactactttttataaaagt tagaaagataactatgattggtctgtgattagacagtattttatgtaaaataaatgggca gcgtgaagttctagcctcagtggagctgccttttctaaagagccctggatcgagcattaa aagagttggatttaatttggctctgccattaatctatttgataaccttgatcaaattatg tatgaacattttaaagtcccttaaacatgctcttaaaatgtccattaaaaagatttcaat ttacccttaccagtgggaagtattagaatacttgaccttgaagctatagaagttagagtt aggaagaaggatgaagtttcttaaagaatcaagttgtaggtgatgttaaaacctctcctt tcactttttatgtctttttttgggggggtgggtgggtaacatgtttttgctaagaatact gttttatctctttgatatccaatatttcctaagtaggatagtaattctggaaattatcct agtggttaatagaatagacctgggattaaaatctggtgctgccaattttttctagacttt ctaacaaagataatgtcattagggagtttattcagtacctaggatattttttagtaaaca cgttttaagaaggttggccattatgttattggtgctttcttcctgtatggcctattagat aagcgctgtgcagtcatctttgttgcctaggcaaaatggtttgtaggttcattgattgaa ctcttactttggaccaagtgctgtactaagcactttggtttttttttttttttttttttt ttttttttttaaagacagagtcttgctgtgtcacccaggttggagtacagtggcgcgatc ttggctcactgcaacctctgcctcctaggttcaagtgattatcctgcctcagcctcccaa gtagctgggattacaggcacctgccaccacacccagctaatttttttgtatgtttaatag agatggggttctgccatgttgcccaggctgatctctaactcctggcctcaagtgatctgc cccgcttggcctcccaaggtgttgggattacaggtgtgagccactgtgcctggcctgtac taagcacttctatgttaattatgtcatttatgagaaagactctaatgattatttttaaag atgagagaactgaagctcagagaggttctacaactcctaaaaatcacacaactgggaaat agcagaaccaatgtcaaaactttaggcatcagcctgctgctcaaaggaagtgctcattga agcgcttcggattttgaaatttctgataagtataatgcaaatattttttaaaaatcccaa atctgaaacatttctggttccaagcatttcagataagagatactcagtttgtatttgtac tttcaattaagctgtgagtgtagagatgaagccaaatttcatctgaagatgtaatataaa tgattacctaatttttttagtcttgaataagaaatgatttgttcacctcttactcagcaa taattcgtaggaaaaattattcatataaagcatatttgtactatttggagtaatgttttc caaagtgtggttcagggctgggcacggtggctcacattggtaatcctagcgctttgggag gtagaggcagatgggtcgcttgagtcgagaagtttgagaccagcctgggacacgtggcaa aacctcatctctacaaaaaatacaagaaaattagccaggtgtggtggcatgtgcctgtag tcacagccatgtaggaggatcatctgagtctggcaggtcgagactgcagtgagctgagct attattgtgccactgcagtccagcctggacagcagagtgagaccctgtctcaaacaaaga aagtatggttcaggaatcacttgtatctatcactttggatggtggttaaaagtaccagtt tacatcagggcacattgaaagaatcaccggaatgggatccagagatctttgtttttaaat aagcctctcacatgattcttggttataccaaattgttacaaccaatgatattgagaatgt tttcctgttttaccttgtttctatgccttgcagcttttcctttaagaatttgtttacaaa ccatagatggtttgtaaaattatccttctgacttatggtgaaatgtaaattggttctttt gtggtactcattttaggaacttgtttaggattaaaagtattcataaactttgactcagaa aacctacttctaggaattatagagataattcctaaggagatactcagattcgcaggaaga cttaggcacaaggatgttcgtaccattatcatctataatggtgaaaaactggaaacaact caatttcaaacatcagataaatggtttgttttttatatccatttgtttggactaaccatc cagccataaaatgttatatggggaagattttgtaagacataggaaagtgctcatgattta atgtaaagtctcgaagaatataaaattatggtcatacatatatgtacatatagtgcatat ggtcttaggttaaatcatatgaaattaccgtttttgtaggtcaagagtagtagaatatca gcattttcatgtagtgtaacctaacatggcacccatttttaaagcacatgtttgtagaga agcacatgtttgtagagaagcacatgtttgtagagaacgctttgtagagaaggagctgga aggaaatacagcaaatttataaagtgattatctctggatgatgatattatggataatttg aaatatttcatctttacatgtcttatgttttcaaattttctactataccttagactcaac ctttgaaataggacaaagtaaattaataaaacagggaacagttgcaaactttctctgaaa aacattaaaattttttagaagattgtaaatcctttaagctagtataaggacccctttata ctaatattcttaactcttgtattaattattgagtgtgtttatgcaaatggatcatatatg cctttacctacccacccagcaaattttgattggattttaatctgcagtagaaagtgtctt tcaaaagaagagaaaaaaatcaattttcaaagtttttaaaaagaattgggttcttaaaag accgccaactatatgcaggagtcagttgggtagccacgttaagcacaaagccctgtttta ggctgtgtggttttgatttttaattgtaatttaactgatcgataaaactctgtagattaa aattagtaatttgtgtatttattttaaaatattactgtatttttttgaaaggaaaatgtt tctcccactttcatacactttaatcgtttaattttaaagaaatagcttaattaaagtaac agctaccatattctggttatctgccatacaccaggttttgggtgctttaatatgtttttt gttttgttttgtttttttgagaaggagtttcactcttgttgcccaggctggagtgcaatg gtgcgatttcggctcaccacaacctccgcctcccgggttcaagtgatactccggcctctg cctcccgagtagctgggattacaggcatgcaccaccacgcccggctaattttgtattttt agtagagacagggcttctccgtgttggtcaagctggtcttgaactcccaacctcaggtta tccgcctgcctcggcctcccaaagtggtgggattacaggcatgagccaccgcgcctggcc ttgggtgctttaatatgttacttctttcaatcttcacctagaatgtattaattagatatg agctttgttttagcgttgaggaggcactgaaactattataagctttcctgagatcatatt aagtggtggatctaggtttttacctcagtaatgtctaacttaaagacccatgttcttatc cctgatactgtccttgttggtctgtctgaaacttttaagtgttcataacttgggaaacaa aactgttgtataagccttcctaattcagactgtcaaagtgtagagcaaagtatataatga gtcattattgctgatttttattcaactatttgttacctgtcctttaatctggtcagtaca tgtgtactggcatttaacgatcgcctgctaatagctaacctcttgtgtaaaccactggaa atactaaaacagagaagccaaaaattacttccttggagtttacagtcctaatgagagaga cagacaacaaaatagttacaaggtatttcagaacataggtatggaatagaacctgttgga acagaaagtttttaaactaataatgtttgggagtagtggtgaagagttacttgggaagcc atcttggaagaggtgaatttagaaggagccatagatttcgtttttgctaccagcctagga atttacattttaatacaagaacccctgcctccagagtgatctgtaattctactgaagttt gagaaccaccactgtagattgtagttgatgctacgagaaagcatataataccctgaggaa gctgtgtggagtgggaaatgagaagttggaaacctcagagcaacaacattgctttgataa gtaggggaagttatatgatagttacgattaatatattataagcaatatcttttagtgcct agaagtaagattggaaatgtcttgaattttgtataagatcacaaaacttacaggaagaat atcaaaattccgattgaggttacttttttctgggagggatggtgaaatgttattctttac cctacaatgtgtgattaagagtgaataaaaatcgtgttaaccttttaaagataatgttta agaaacaggtttaatttttaaaagaaaagtgagcataggagcaaagttttgtaacatatt ctgcttctatataggaaattaattcctaaattttggaaatgactagttgaaagtaatagc agtctatcctccaaagagagctttttaaaatttattttttagaatcagacctgggttaac ttttagatataatttggcataaccagcaagtcattggttattcaaaaataaagattggaa ataattgccatatagtcttggctctttgcgtattttgtgatactgattgttgcttcctcc tttacttccattttattattagagcttcattaagagtgaattgaaaagaaaactgaattt tattattttaaaaatctgaatagttggattggggcaactttcttcctatttatgagactt cactcatttatgttggtcttatattgacattaaagaatcagtgtcatatactggtgaata ttaacaaagagacaagattaagattaaagaaattgaacctttaagcaggaggcgtgtgac taatggagcaaatttctgaaaggcttaggggtagagaaaatcaaagctactgatggtatt aatagtcttagaaatgatggagattacatttttcctctcagagacaggcgacaaagaagg gaggagtaaagaaacaaaacctttgagattgagatatcacaagttgataggtacacagct ctctgaaagcttcagtcatctcaataaggagaggaacatacttagccatataagttcgag ttgctgaatatacattctatgtaggtaggtctgtgtaattttgatacgtattttctgttt atcacagtggaaattgttcttgatcaatgtttttatttcaataagaaagatatattgccg cttccatcttaaaatttctacatagaagagacatgacgcttagtgaacgttattgaaaaa aaaaaaccgtataaacttaagcaatgaagaaagagaaacagtgtttcagaatgttacagt attgaacctgaattggtagagagaatagaaagtggtgtgaacagtcagggtccaaatgac attctatataaagcatataggacatgatttggagcagggagtgggatggggaatgcttag attacatctctttactagatgggtgtacttgttctgtgcaaggtaccattttctcccctt ttttgttagctcatatatcccttcccagttttgaagagtgctccactggtatttataact ttagtgatttgacatttcaggatatacctgtttcagaaaatcataagaagttttaaaaat gccttgatgtaaaacaaaatttcaggtaaacttaaatattttaagtttttaataatttgt gttaccaaaattaaattttggttagagtttgacattcctaagtgatagaatcatcttaaa cacatgttgtcaacagaaatttgaatgctgaagtcaatttttgttattgttattgttctg cttagtgaagaaatgtatcctctctaaccagggacagtatgtactgctttctccttaata ctttgagattttcataaagaaaataatttcaaaggtgttgtgctgcaagctaaagagatt atttctaaaggaagaatttgtcattctaatggttgtcatcttccttgtggcattattata aaattaagaaactgaaaaaaaatttttttagaagacatagtatataccagaatttaaatt tagtttggcataagaggccacatattctacctatgctattacagtctgtatttgttattg cctaattttgactcattttgaatatctcactccagtcctttttattagtatttcttaaaa caaaaatgggcaatatgttggattgactgaaaataatttttaagtgtttagtagcaataa aatttaatcttttagtgcagattggtttaccccataacagcacatatggaagaagtaaag ccaggtctgtatgaagcaggaggtgacattggactttttttttttttttttttttgtgac ggagtcttgctctgtcacccaggctggagtgcagtggcacgatcttggctcactgcaagc tccacctcctgggttcacaccattctcctgcctcagcctcctgagtagctgggactacag gtgcctgccaccacacctggctaatttttttgtattttttagtagagacggggtttcact gggttagccaggatgtctcaatctcctgacctcgtgatcggcccgcctcagcctcccgaa gtgctgggattacaggcctgagccaccgtggacttctttaaataaccatagtacaggcag ttacttttgttatataacagtatagttgtctctgaagaaaacctgaagctctgcaaaatc gggccctgaaaatcggtttctgagtggcttgttcctgtgtaaacttgtaatctctgtagt aacgaaaccagtacccattctaataaaaatattatatagctaaatatttaccccagttag ttctaagttctaggatttatgctttctcctttgaaatgtaggtcctcgagaacattcatt tctacttatgatgaaattgtgaaaactagtaaatctgatccagggtgtagtttttatcag ttgcctctcaccccttaaaaaaaaaaattaacgtgggaaaaatattgtcatggattttct atctgtactcacagcttgaccagatggttttaacattgtggaatgcctagcagtttaaag ccattaactagcctctggttaccctcttctggatttctagtttttttcttaaggtcatca tgtattgcttaagctctgtctttgtgagaaagcttgtccctgctggctttaaaactttac tatgctggggaaaattttggactaaagtgacattcacattatactgacctcatcgcccaa atgatgaatgaatgaactaatgtacactacaagaacatgtcttaataggaaagtagactg ttgtctgtagttgtcccattgttttatcattgctgctactatattgacatgaagtagcag tgggtgtgcatgtttgtatattttttctgtgctgttaaataagtccacgcacatctccca tgtgggtagggtagtactgggtgatggagtaggtttaccagtaccatttgtcccttactt tacttgtatagctttatgttttaaaatagagtgtttttgtttttttgagacagagtctca gtctgtcacccaggctcgagtgcagtgatgtaatctcggctcactgcaacttttgcctct ggggttcaagtgattctcgttcctcagcctcctgagtagctgagattacaggtgtgtgcc actcctggctaatttttatgtttttagtagagacagtgtttcaccatgttggccaagttg gtctcgaactcctgacctcaagtgatccacctggcttggcctcccaaagtgctgagatta caggcatgagccaccgtgcctggccactttttgttagaattaatactttgaagataccat ggatttggttctagaccactacaattaagcaaatatcataataaagttagtcacaagaat ttattgtttccccagtgcatataaagttatatttacatggcattgtagcctattaagtat gcaatagcattctgtctaaaaagtgtgcataccttaactaaaaaatactttactatttat ttatttattgagacagtcttgctctgttgcccaggctggagtgcagtggtatggtattgg ctcgctgtaacctccgcctctcaggttcaagcgattctcttgcctctgccttccaagtag ctgggattacaggcacccgccaccacacccggctaattttatatttttagtagagaccgg gtttcaccatgttggccaggctggtcttgaactcctgacctcaagtgatctgcccacctc tgcctcccaaagtgctgagattacaagcgtgagccatcgcgcctggctaaaaaatacttg attgctaaaaaatgctaacaatcacctgagccttcagggagtcacgatcattttactgct gtagtcttgcctcagtgttgatggctgctgactgatagagtgatgttgctgagggttgga atggctgtgtcagtttcttaaaataacaatgaagtttgctgcattgattgactcttcctg tcatgaacgatttctctgtagcgtgtgatgctgtttgatagcattttatgcagagtagaa cttctttcaaaactggaggcaatcctttcaaatcctgccgctggtttatcaattaagtgt atgcagtattctaaattgtttaactttgtctcaggaaactactttccttgctcattcaga agaaacaacaccttatccattcaagttttattatgagattgcaacagtttagtcacattg tcaaactccagttctaattctagttctcttgctatttctacgcgcctgcagtgacttccg ccactgaattttttttttttttgagatggagtctggctctgtctcccaggctggagtgca gtggcacgatctcagtcagctcactgcaacttgcgcctgccgggttcaagtgattcttct gcctcagcctcctgagtagccctccactgaagtcttgaacccctcaaagtcatccacgag ggttggaatcaacttcttccaaactcctgttgatgttgatattttgacctccttctgtga aacacagatattcttaatggtatctggagtgcagttatccttctcaaaaggttttcaatt tactttgcccagatccatcagaggattcactatgtatatatataaaacagctaatgtatt tctaaaacaagactggaaagtagaaatgacccttgattaatgggctgcagaatgggtatt gtattattaggcatgaaaacaacatgaatctccatgtacatctccatcagagctcttggg tgaccaggttcgttgtcattgagcaatgagtaatatttgaaaggaatctttcattctgag cagtaggtctcaacagtgggcttaaaatgtctagtaaagtcttgcaataaatggatgtgc tgccatctaggctctgttattccatttgtagagcacagggagagtatatttagcctaatt cttaagagctctacagtttttggaatggtaaatgagcactgttctacctaaagtcaacag cagcatcagcccctaacaagagagtcagcctgtcctttagtttggaaggcaggtattgac ttcacctgtccagctagaagagtcatggatggcatcttccagcagggggctgtttcatct acagtgaaaatctgttgtttagtgtagccaccttcgtcagtgatcttagttagctagacc ttttaggtaacttgctgcagcttctgcatcagcatttgctgctttaccttgtacttatat gttatcaagacagcttctttttttaagcccgtgaaccaacctctgctagcttccagcttt tcttctgaagctttctcacctctctcagccttcatagaattgaagtgagttagggccttg ctctggataaagctttggtctaaggaaatgttatggttgatttgaccttttattcagatc actcaaaactttttccatatcaacaataaggctatttcactttcttatcatttatgtgtt cactggagtagcacttttaatttccttcaagaacttttcttttgcatttctagcttttga tttaaagtgagactcttcctttttgcctgaatgcttagaggccattttaggattattaat tggcctaattttaatattgttttgtcttaggggatagggaggcctaaggagagggacaaa gatgggggaatggctggttggtgtagtagtcagaatacacaacatttttgccgtcttcca tgggtgcggtttgtggcaccccaaaacaattacagtagtaacatgaaagatcactgatca ttgatcatcataatagatacaataataatgaaaaagtttgaaatactgcaagaattacca aaatgtgatacagagacatgaagttaccacatgcttttgaaaaaattgtgctgatagact tgctcaacataggattgccacaaacttccaatttttaaaaaaatatactatttgtgaagt gcagtaaactgcagtccagtaaaatgaggtatgcctgtaaaacaaacccgcagaaaaaaa atcgtctactcagaagagcactgacaagtatcattgaattattatatctacaacttgcta atcaagctaatgtagtgtgagttgtagatacaaccattttaatgcaggggttcctgagat ctgaaaattattttaagtgtgcctccagtgtagaaagattgagaaaggttgaaagtgaag atcaataatgggatgaactggaaatatgcctacctgataatacaaagcaatgagagaaac atagtatcacttccgtcatattcctgcctgcgatgcataatgtgaatccaaaattagaca aaccaaaattaagggccgttatacaaaatgaatggcttataatctttcaaagttgttaaa ggaatgaaaataaagaaaggatcaaggaagtatttcagatacaaaactcaaatgggttct gaggaatagacagtctgatatgtcagtgccattttccttactttaacatttatattgtga ttatgaagaagtatgtcctttttataagaaatgcatacttacgtgtttggtaggggatgg tgcatcatgttggaaacccctcccctcccctcccctcccctcccctcccctccccaggcc tctcgttagtagtttccaaaccttttgcatgctgaacccattgctcaaatgaaggcagaa tattaaacatgtaaaggaagagcttgtgtgtgggggggggggagcggggggaaaggaggt tgggggaacagagaattaggaacccctgagtgactacagaacttctgaaaaccactgttc tgtaggataaaatctaaactctgaagcacattatacaaacctctatctatacacatcagt ttgtctgaaattgtatatttctcaacaatattgatctatttgtatttcaatatgttgtat gctctcatatgtctgtatctttgcaaatgatattttcttctctttaacgatttttgtctt accctgctggtaaggaagtcaccttccctctcccttctgtatatccttgtcaaggaacta tggaaattgaatggcataggtcttgatacaagaacctctcttttctctttataaaaaagt cagtaaatagtggtggaacattcaggagaaagttggggacatagagactaagaaggcaat gcagtattattaggacagggggtaaactaaggcatagaggacagatttgatgaacatatt atcttttttatatacattataagaagtagaaggctagaaaagtgaacatgcttgctattg cacgtctcactttccaatttcatgaagaaagcaaaaactacttctactttattacttctt tttgatgttaattggtaccacatagcaagtttgcattatagtactgagctttaaaatgca aggatatactcatgtatatacacagacaataaataataggttcttttccacattcaggaa gtgaaactcagctgtccgtacgacacttactgtccatatataggcatccctcagtatctg caggggattggttccaggaccctcacaccaaaatctaggtatactagtcctgcagaacca acatatatgcaaagttggtcctctgtatacttgggtttctatatcccattaatgtggaga atacttttgatatgtggatatggagagctgactgtatttattgaaaaaaaatccacgtat aagtgcacctgtgctgttcaaactcatgttgtttgagggacaactgtattctgttacttc ctatagtttctaaaagcattaaccaacaaatacatgtaagatgcgtggaataatttttcc atttcctagaaaatagtcaatcatttttttttcagattgtgaactagaaagcatagtgaa tatagatttttaaacatgaaaatgtaagtgtccattttaaattgttatggatatttctgt ttgctgaagttcaagactgaagactactggaaaaaatcctgtgactgagagcacatagat tccttctttttaggtagagcgcaacagtcaccatgacttcagtgtttgcatatggaagct aaaatctgtctgagaaagggcaaacaatcacaggagtcattgatttatttatttgttcat ttttaatgcaaattttaattgagtgaaaaagtggtggaaaaaacagttgactaaagaaga tatgagaagggttaatacatgaagaagtcctcaataatattagccaccatggagatgcag attaaaaccacaatgccatactactttccatccacaagaatagctaaaattaaaagaagt cagtcaccactgaatgttggtgaaaataggaagcagctgaaactctcttacatggctggt ggaagtgtaaaatggtaccattcctgtggaaaactaattgacagtttcttagaaaaaaca ctcaccctatgagcaagcactcctagttatttacccaggagaaatgtccacaaataggct ggcacaagaatgcttatagcagctttattcagaatgaccaaaaactgaaaacaactcccc aacattcatcaagagaagtgataaataaattgaggtatattcatacaatagaaagtacac agggacagaaaagaatggatttctgatacatgcgacaacacgaatatcaaaaacagtatg ctgaattaaggaaaaagacaacaaaagtaatgcagcatgagtcatataaacatcatataa aaatgatgtttcaaaatccacaaaactaatctgcactgatggaacagtagttatctaaat gtgctaaggagtgattagaatgggaatgggacatgagaaaagagggaactttatggagtg atgaaaatattcaatacctttttctttttgagacagtgtctcgctctgtttcccaggctg gagtgcagtgtcgcgatctcgtctcactgcaacctgtgcctcccaggttcaagtgattct cctgcctcagcctctttagtagctgggactgcaggcttgtgccgccatacccggctaatt ttttttttgtgtttttagtagagatgggggttcactatgttggtcaggctggtcttgaac tcctgacctcaaatgatccacctgccttggccccccaaagtgctgggattgcaggcatga gccaccgcacctggctgaaatattctgtgtcttgattaaggtgttgtatatatagatata tatgtttttcaaaactcactgcgctttctactttatataaaaagtacctcaattaaatta catattaattgaaggcaaattaaaaacatctctgtctgtaactctcctactgtgagtctg ttccatgttgaagaatgctcttgtcttcttttctttgctcttcttggttgggacttccct gttctaatatcaggtatgctcacgttagaaagctaggacagaggcctagaatactgattt ttttccatttcaacaggcaaggtgacagttttcctagagttcacaactcttggtattcca agtcagcacatagttcacggtgaaacattctctaggaggtagagagaattgtgaccgaag aatatgataggaccaaataagtagctatactagaaggaagcacatttgagaccactaccc taaacagccaatgactacagaaccagagaaaaccagaatctgccaaatttcagaaataac tcagcatctttacagggagcctctacacaatcacaatgagactctgaagagtagcaagta ctgattcaaatccttttagctgtggactccactctgcctcacaatcattttagaagaggg ttttgagctttagaataagcccaccactgtataaaactagtgagtctgttttttaaggta ggattcccatcttagtaatcataattttggcatggcaattattaaatatgaaccacaagg agaaaaaaatgtcctcttgttattactttttaaattaggcattttaatttcatttactac tgcttgatactctcttctctgggttcctgggaggtttcatttggtaagtattgttcagta aaaattatgggacaccattgttttggactgtgctcctgccctaggccccagtggaccaga tcaaactaaaatgtagtcattcatgttaactgccacataatcaaatagaaacttccagga agcagacagatcccaaagcagaccatttttttccctgagaacaggagattccagtctagc tgaatcccctgtgctttaaacccttagaaaaagtaaactgaagtaacctgatattaacaa atcagcttttttttcctgttctgtttccttgttcccaccttacaaaacccactgttgtgc tgttgctcagtaggacctctcattctaccttgcagaatgggggctgccctgattcatgaa atgcaaataaatgccagttagatctgtaactaaactgttgtaattttgtcttttgacaga atgcagttaaaactcaggtatatccacactgcttttatttcgtctccttcccatttcaga aactcctgtctagatttcttttcttgctctctaaggcaaacaattaccacagttagatga atatgcctggcaatcgctattgacagatagactctcagaaacactcgttccattttcctt ttcaaattcttggcaggaagactgggtggatatattgaaggaggtactgtttgattgtac tgcacacagtgtggtttcaaaaatgtgtatttgaattggaaaagagaaatatatgtcagg cactggggatacaatactgaccaaaggtatataccctgaatccaaaaataaatataatgt agttgctgtaaacatattaattttaaagattgtcagaagaatatccatcttagttagacc tttgcaagatatatagataagaaaatttctgaaagttacataactaaaatagaaaccagt caaaatgaacttcattctaccctggtttcttccctctaatgttcttatctgtcaatactg tgactgaaaaagccaaacctaccaccaccaccaccactgacaaaatccaaaaaactacag atatctacaacattaattctaacaccaagtaacattataacccacttaaaactgttcttt tttctttctttttttagtacagacagaacttttttttttttaatggtaagaaatatcatg attttcttataaagaccttctgtatgtacaaataaagctatactatcatagtggataaat cagtaaacaatatcttatgtaatctgtaatttttcacacaagaaatttaaaaagtttttc aggttatagaacagtatttctggacacacctaacaaggcagtagctgacattctcaaact tcaaaacaccctcactttcaatacaaataatgctgtgttgtacatttaacctaaaactaa gagaaagcattgttggttggccacaacttgaatttcttattacaatgaggactgcttcag gattttaccactaaactcagccgttcagagaactaggaagatagtaaccatggtcactgt tcttcaaaagcactgatttcagatttgttgaagggatatttcttagatcttcaagtttct gaaaaggtttccttcattctggttttctggatttccttgtcattctgaagttattcctct tgcacaggcaaaattagagtactcattcaataacaacaacaacaacaacaacaaagccat aaaatatgccttcttttttctacaacaccaatttagctactcaagatacagaactaaaat attagcagaaaatcttgtaggtatgaagccagttatgtattcacattagtaaagatgatt ccactgcattcatttaatcagcaaacatttacagaatgcctactacataccaacaatata ttagtgactgcatattcatttaatcccgaactctgaaatcattattcctggtttacaaaa taggaaacaggcttggattaaggttgcttgcccaaagatttatagttagtaagattcact accaagtttgaaactcagatatgtctgagccaaaatttctgctccttccactaagccaag ctgtcttccagcatggcttccagcatacttagtatcttgctgtatcaagagaaaacaagg tacaggtgacagtgacgtgatgccattatgactatctctttttatataaaaagacccttg ttgagataactacaactctctctttctcagacctcctacacattgcttttccttcaatca tcagggtcatgagccttctcccctctcctcccccgccgccccccaccacccaacttcccc ctaacaaagtaaaagaaatcaaatgagatttgattaaagtgttttgtctagactgcttca ggactaaagggtcttggtaatttgtaccagcacctcacttcttccccacgcccaaaaact cacgtacacagtttgattgttcttgctttctaaagtgttctcttttttttgagatggagt ttcgctcttgttgcccatgctggagtgcaatgacccgatctcggctcaacacaacctccg cctcccaggttcaagtgattctcctgcctcggcctcccaagtagctgggattataggcat gtgtcaccatgcccagctaatttttgtatttttagtagagacggagtttctccatgttgg ttaggctggtctcaaactccctacctcaggtgatccacccgccttggcctcccaaagtgc tgggattacaggcatgagcccggccaaagtgttctgaaagctatgtgtgaatctgttaag tacaatctatacttacatttgattataaatattttgacgatctgaattggtaagatttta ggcaaaacaacaaaatcaaatcccgtggactctgacccaaagaatatattcagctttgtt tgaatatatttcatcttgcttccttaccaattctcaacacaactccagaataactgttat taactcttattgaatcttattttgtactaagccttgttaaatgttttacctatgaattgt gatttcattgttacagcaaacctatgatatagatgctatataacagatgaagaaatgagg ttcagaacctgtgctacgctgcctcgcaggcacaatccatctgataaaagtagtcccttg aatctctctttctgaattccagtcccaggacagtggaaatacacagtattgtggtcaaag ataaaccattcttggctccaggtgagctaatggttttagataagaataaagaatatttat tagaaataaaaaattttgtaagtataaaattagactaggaaaaagctcccggttgaattt tatatttataaaatcctttactgaagttgaaatcttctccttgaaatttctgcttggtat ggcctctctgtttgcctcacaagtgaaatttaatcctaatgttaccttattttccaagca taaccacaccagtaggctgctttgtcatgagtttagtatggtgtcagtagaaacgagacc agcagtcattagggttacctaattcaaatcttgactcattttgggtacttcctacaagat tagaataattatcttccaaaccaaaaacatacccaaggttccatgggaaagggtgtagct tcaactctttttaggtggcaagaaaatcattctctaacctctcaaaggtcaggaataggg tcgatggtccttaaaatttggtctggggaccactggttgctgaaccttttgcaggaggtc tgaaaggtcagacctgttttcacaatacatgttaagaagttaatggcttttttgttgtca ttcttttccaagtgtatttgattatacaacttgggatgatgttcctctgatagttaatgc aattatacttatgttttaaaagtttgttagttttgatttctaatacagtaagtctcaaca gatagaactcacataacaaaacacttttgggagtccttcataatttttaagagtgtaaag gaggttctaagaccaaaaggtgtggaaactgctcttctaggtttcagaattatgaagcat acaaagactcttcatctggaagggtttagaggtaattttatctaatccccttattttaca ggtttagaaactgattctagacatttaagttcccagactaatgtcacagaagctaatgaa ttgcagaggttaattggaagcctggtcttaacactcccaggttatcttaatgagttcatg aggatggcatatggataatgcacttcaaagggtgttgtaagtattaactaagttaataca ggtcaaatgcatatattagcactcaatgcacggccattgatcaataaatgctagtggttc tgatcagtgagaatctaacctctgcttaaatacctttagtcatcagcagcttccactccc tgagtaacatgttgcatttcttgatcaattatatttttacagaattcttcctttactgaa gttgaaatcgtctccttgaaatttctacttggtatggcctctctgtttgctacacaaata aatttaatcctaattttatctagcttattttccaagcataaccacaccaatttcattaaa tgattcctcatgtggcatgactttaaactccgtcaccatcctatttgtttttctcaaaga gctccagttgactgctcctgtgaaattgtccatctattaatgtaaatgttttttctaatt ttacagagctccccgttgtattgtgtacagtgttaaaatagttttctgagatttcttgac tctgttttcccaagtttcttgtggcccttctctttccttcgtctctattctgtgcggttt ttatttcactcccacagtttctcattgctgtgaggccctgttatggaatgagagccctgg ttttgaaagttcacagaggctagacttctcttgtccctgtagtcctggctgagggcccac tacacttgtctgttatccgagtgggcaaacgacctacccgttttcatctgctgggcggcc ggttatttggggggatccccctgttacaggtctgatctctgttgcttcctttgggaggcc gaggcgggcggatcaccaggtcaggagtttgagaccagcctgaccaatatggtgacactc ctgtctctactaaaaatgcaaaaactagctgggcatggtgacgtgcgcctgtagtcccag ctactctggaggctgaggcagaagaatcgcttgaacccgggaggcggaggctacagtgag tcgagattgtgccactgcactccagcctgggcaacagagcgagactgtttaaaaaaaaaa aaaaaaagtttgtagccattatctctggaaatatttactcttcctcattcttctgcttat ctctttctggaactccaactagatatatactagacctttgattctatttttccacacctc ttaacctgtctagcttatttagtcatacatctctcaagctgcattcttccttggagcttt atctgtaggaattctttgagccttgattgagtttatattgcttcagagaaaactaaggtt tgcttcagctagttgcccaggaacattaccacctggttaccacttttaattaaggtcact gcttgaggatttcagagcgacacagagccctgtatgagggcctgtttatggttataaatt cttggggtgtaggcaggggtggggatcctgcttctttacctggagctaaaactgagacag aaaaatccctgactgtccatttttgtgtggtgggtttattttctgttcaccctgaacaat gataggtgttcaccttgtagagctccccgctttatgtgggggtttcctattagagaacta tcatatgtggcctgttggcttattttcttgccttcagcacctgtgccagtacataagtta gaaacccaaggtttccaggtttcgaaaaactctcagggcaaaagttcgttttagcctatg cttagctcccagggtcctttgtacttggccatatggatttctttttttttttttcttcat gtcaacactgtggttatatttatctgtctaacctactcttttaatttttgttttatctgg tattttaattgtttcatttgagaaagtttttcatagtatctgtttggtagttggccattc tactaggagtttaggagttatacttaactccttccagggagatgttggaagatttttact aaaaatacaatatttaaggtgggtattgaagtactatcagaggggacctggggtggagta tgaaaacaaaacaaaaccttacaaacgggaataattaagagttaggatatgtgagatgtt tggttttgctgggcttaaaatagaattgtggggtatggaattgctgcaaatgaggaagat ggactcagagatgaggctcaagagaggccacatgcaacttttgagagtctttgtgtgtca cagtaaggaagatgaacttgtgaaatcaccaaagggtggaatatataaggaggaagctga cactgtcatatctatgccttaggatgtttattttggcagcagtgaaaacaaatcaggtag acaagagtggagcattgctcttcaaaatttagtcattgactggcagtaatggcatcatct gggagcttgttagaaatgcagaatctcgggtcctatttcagatttaccaactcagaatct gcattttaacaagactcctaagtgtttcatatgcatagtcagttgatgtctcaaccagtg acctgcagagccagtgatctgcaaagcaagtgaacgttagctttattttaaatttcaaga aagaagtcttaatttgcctagagtacatatcatttgatgttcacagtgtcactttatttg cctaataactagctgactctagatccttagcagcattagatttagtatttgaggttttga ccctatgtacctttgataagtagtgatttgtaacttatgaattaaatttgaattccttaa catgttgctagttacaaaactctagtgtctctagcagactattaagaaattgagcaggtt tcgttctccattcactgttaacacatgcggtaactcaccaggcgcttaaaacaagtgggc tgtggtgcagggtacttcttgtttagtgacagaaatgaaaagatctaagaatatgatggt tttaaataaatatgataagagatgaagagagttttaagtaatagaactattctaagtttt ggatgtttggtttagtgtctctagctatatttctgtttaatgtcaaggcataatatattc gaaccattttatttattaaatttaataaacattgattgtcctattagtcaaagtttcaga aaaatgcaaaaagtcttgttctcaaaggaatgctccctggaccatgcacatcaaagtcgt gggtggctgcttgtgggggtagaaaatattaaaaatgcagaaactgggccctactgccga atcccaatgtacattttaaaataagcagcttcctggccaggtacggtggctttcacctgt aatcccagtattttgggaggctgaggcaggaggatcacttgaactcaggagttcaagcct gggcaacatagtgggacccccatctctgcaaaaaatggaaaaaaagtaaaagcatctccc aaggaattctgccagcaggaaagttgtgttttcccccctcaaatctggaaaccataattg ttcactttttgaccttttactggaatacagcatactctgagaaaagtaacaacactcaaa taaaaaaatagaacattctattccagaacctcctctagtgactccttctagaaagcagtc tagcaccccacccctccaaacacaaacataaccaccatagtgacttttaacaccatagat tagtttttccttttttgaactctttaaagtggaataatacaatttatactctgtctgttt tgtgttgtttttgcttagcattatgtctgtaaaatttgtctatattgttagctgcagttt gtcattctcattctcattgctgtgtggttctctgttgtttaaaagtaccagtggtaaaat tatcatttatgtggtttatctctgatggacattagaggtatggtggtaccactgataggc accaaaatcaagtttaataaccactgttgtatattatttgggaaaatgtgtactcttgac atgtttggcaaatttttatttctccaatctaaaatttagaaaattataattttaaaatta tcaataaaaatttacaaagtttttactgtcaaatattatctaatagctcaactagctcat gctagagtaatacagccatgtaatacagcattaccaacttttctaagtggtctatagcac tgaggaattaaatagcaaataagggaagcatttcttcatgaaatcatttcttttaataaa ttaaaaagaaatgagagtattagaataccaccattttgccaccttcagtgtattaatgga tggaggcaatgaatatcaacagctgctaaaatcataagaaggaaaccagatgctagatga cacataatgaagtaacacattattagacaaaggaacaaaccaagttcaataaagcctctg gatccagcttccaatttgccacaaataaagagaccagatgaatatgctgaattgcattat gtgtaaataatcagtcaaatctacactacgaaaagtcagatcaaacataaattttaagaa aaagaaaagaatggaaaataaacctgtagattaaaagagactgaatagaagtagcaactc ttcttaaaaatggtcaagattaaataatagttatctagggaggcttacttagctgataaa gtgataaatacaggatgtaattaagaataatttaaaagaagtgagttacttagaggaagg gaaggggctatgactgaatcagcacttggaggagcttctgggctcgctggcaaattgctg tgtggtggtcacaagaatgtattctcttaatatgctaaggtataaagtttttgtatagtt ttctgtatctgtattttattttactctaaaggaagtagttttttaaaaaagattatagat ttgctaatctaatataaactcccctagagcttcatcagtaatagtctagtgtcatctgga ttatcttcagcaatcattttcattctaaattgtcatagtgtcatctcaaagcagaaagag acagagagtttgacaaagtgtcttaaaatgcctgatgtctgtcttaatgattctaataga aactcttcagtacaatactcagacttactgcgcttacaactacataatggcttataaatt tgggaaactcaacagatttgggaatacaacacatatcttgaagccagttaatttactttt tacatacctcttcactattccactaccagtgtgcaactggtaattcagttgaattcataa agaattcactgagtttttctgaatttcttctgatttcaagaggctagaagaactcagatt ataaagcatcaggttatcacttttactatataggcacaccatggtttattgcactttgct tctgattttttttttaaacaaattgaagggttggagcaatcctgtgttgagcaagtctct tggtgccattttccagcagctcactgtgtgtctctgtgtcccattttggtaattctggca atatttcaatctttttcattattattatacctattatggttatctatgatcagcgatctc ttttatgttactatcataattgtttggggatgccatgaaccacacccatagaagaataaa agatggcaaacttaataaatgttgtatgtattctgaccactccaccaaccagctgttccc atctctcttttctcaggcctttttattccctgagacacaacattgaaattttgccaatta ataaccctacagtggcttctaagtgttcaagtgaaaagaagagttgcatgtctctcattt taaatcaaaagctagaaatgattagtgaagaaggcatgttgaaagcagagacagggcaaa agctaggccttttgtgccaaacatccaagttgtgagtacagaggaaaagttcctttcttt ttttcactttttcgtatgtttaaaaagtagagacagggtctcgctatgttgatcaggctg ttcttgaactcctgacctcaagcagtcctcccacctcggcttcctcaagtgctgggatta taagcatgagccactacacccagctgaaaagttcttgaaggaaattaaaagtgctactct ggtgaacacatgaattataagcaaaacagccttattgctgatatggagaaagttttagtg tctggatagaagatcaaatcaaccataacattcccttaaacaaaagcctaatccagagca aggcccgaacttacttcaattctatgaagggtaagaggggaggaagcttcagaagaaaag ttggaagctagcagtttggttcctgaggtttaaggaaataagctgtctcgataacatgta agtacaaggtaaagtagcaagtgctgatggagaagctgctgcagcatgttatctagaatg tctagctaagatcactgatgaaggtggctaccctaaaagattttcaatgtagatgaaaca gcactctattggaagatgccatctaagacttttctagctggataggggaagtcagtacct ggcttcaaaacatcaaaggacaggctgactctcttgttaggggctaatgctgctggtgac ctcaagtggaagccagtgctcattggtcattccgaaaatcacagggtcctaagaattatg ctaaatctactctgcccatgctctgtaaatggaacagagcctggatgacagcacagtttg ctagatatgttaagcccactcttgagtcctactgctctcagtgaaagattcctttcaaaa tattactgctcactgacaatgcacctggtaacccaagaactctgatggagatgtacaagg agattcctattttttcatgcttgataaacataataacatctattgtgtagcccgtggatc aaggagtcatttctactttcaagttttattatttaaaaaatatatttcttaaggctgtag ctgccgcagtgattcctgtgatgaatctgggcaaagtaaaccaaaaaccttctgggaagg attcaccattctaagtaccattgagaacattcgtgatttatgggagggggtcaaaatatc agcattaataagagttgggaagaaattgattctacccccatgggcaactttgaagggctc aagactacagtggaggaagtcactgcagttgtggtagaaatagcaagagaagtagaagta gaacttgacacagcgtgacaattgctgaaatctcaagataaaatttgaatggctgaggtg ttgctttttattgatgagcaaagaaagctgtttcttgagatggaacctactcctagtgaa gaggctttgaaaattgttgaaatatcaacaaaggacttagaatattacataaaattagtt gacaaattgataaagcagcggtggtttgggaggattgcctccaatcttgaaagaagatct acagttaggtaaaatgctgtcacatagcctagtgtctttcatgaaaggaagagtcaattg atgtggctaactttattgttgtcttattttaagaaattgccacagccatactgattagtc agcagccatcaatatcaagataagaccccccaccggcaaaaaaattggactcactgaagt ctcagttgatcattagcatttttttaagcagtatagtccttttaaattaaggtatgtaca ttttttttacacataatgctattacacacttaatagtagtgtaaatataacttttattgc actgggaaaccaaaacattttttgtgacctgctgtattgcaatattacctttattgcagt ggtctggaattgaacctgcagttttctgaggtgtaagtattggtgaaatcattaacctcc tcctctgtgtgtgtggtttcttcaactcacaaaggatagcagccccagtgaaagtcgtta cagaaggcagataaactccattttttttaagactttgccactctgtgtgtgtgtgtgtgt gtgtgtgtgtgtgtgtgtgtgtgtgtttaccttatcctagtgggccttaccacacatttc tattatgtgagatccaaaagtgatgtggtatacacaagagtaattttcaccccattctta aaactgaaaatgatgtttaaatcactttttcttgggggaaaattcaaccccgtggtaatt aagcttcacttttcctgaaattaatctttttataaattttataaacttataagaaatgta atacattttgcttaagtaactagcataagccaaaatggcacttcatttttcttaggctca gcagatttaatttaataaagattataaattgattttaattaatgatgtttacgaaatgaa aattaattgacagtgttgaatagtaaagctgctaaaaccataagccaaagcaatatgaat aaaagtataatttagacacaattttaaaccagattttgaaaaactttactcggtgatatg gtcaggctcaattgtatgcctgctagttagattacttcttgtataggctgatatatgcat aaaattattatttttgaattttaaataaaattatatttatctctttaatgtgaatttacc tctttaatgtaaaaattatatttgttttttgtccttgtttcttactgcacatatcaattc caggtcaagcatgagataagcaaatcatgttaatttcattttcaactgaaataagactat ttctgagcttattcttgtttgctgaataaaaaaagattgttcatatgtgcgagaaattgc tgcagcataatgttcactgtttttctgtgagtagcaggataatcttttcttcatagaaat ctacatcttgtctgggaaagattagtaaaactcatctttaatgtatgataaaactgcaga tctttttccttttttctcaagtcaaattttcacagtcagaacattcagaaacaggatccc ttttccagttctacacttgtgttcaagtgaaggaaaatctgttcagttttttttctgcag ctcctccaggtgatttccagtaagacttgagacttgctgatatccttcctcactctcaag cccaaacagcaatagaaacatttcaagattttcttttgtaacatgtttttcaaaagctca ttttccttttacatccaagaatgttgtcctttgaaataaagaaaacccaaccattttctt cacaccttacagagactgaaaaataagtactacgtaggctagtttctacagccattaatg tttaagtattcaacagactgtagcctactgttttctttaaaggacccatttcagttcaaa taccctattgagaagttttccactgctaagtggattttgtgtgtgtgtgtgtgtgtgtgt gtgtgtgtgtgtggtaagagctgttctttaaatgagtgaaatgacctttgtttaatgaag ttaaccattttataacatctttcagatgttaaaacattttgccccaagagcttttgatat cagtactcttaacacagatgttttttgtgttagtttgggtcttctgagacacagatgtca agacaagatttgatgcgcagggaatctcccaccccaccctactaccctcctattgtcctt tggagcaatccccattctagtacttctgccctgcttggtagcttgctgggagaagcccag aggcagcaaggccaggggtggttgccatggattccaaggtgcgatagatggaggctgtca agcagttctgttcctcatagcaggttaggttgaggggatatctgagtagggtaccactgt ggccactgcaccctcctcttcagcctttgacatctttccctcatgtggacactctttact gtgttacgcacccacagggatgttgtcactgtgttctgcctttgacctacgggggcttct ttactatatggattattcctcatgcttcttagagtcaagctttgccaccttgcccccttg ttggtctgcctctgttcctccccgccgccatccctcactacagaaaggctcttttcattc tgctttgactcccaaattttggactacctgcctgccacatacatacctaacttgcttggc tccatctaatagctttttgactaaatttttgaggaaaagaaggaagaagagccatctggg tttcttacatctgcaactagctagaatatgattccattcactgaaatagagaaaataata ggaggctctaagaacaaatttgggccttacgtatgtgtgcatgttgtggggaaatagaag tttggatgttaaaataatgaatttggtgtgactttgggacaccttcttgaaattaatagg ctcagtagaggagtctgactggagataatagtatggggagtcattagcttctgtgtgata gttggaaccctgactttcaacgaaacaacaaaggagggaggagctagcgatggagcacag taacagtgctgcctgagggatgcactgaggaatgtaccaaggaaaattaagggacaggag agagttgtaaagagagctagtgaccaacagaatataaagatacaaaaatggcaaaagagt ctttgagatttaacaaccagtttaaaatttagttattatggagtaagcccatatcacaca ggtggtggcgtatcacacagggattaaatttagccaaaacaaaatatgtattataattca aataacaaaaaatatatattgtcatgtggactgaggaatagtcttttgcatcctttccag taaagatatatatgattgactagtgaagtctatttaaattattcagtgtacatccataaa actttatcaattactttctgtgtatatgatgacacttgccagtattgcccattttgtgct gattttgttagtgtacattactaaggagcttttcatcaaattttgcagtccctgaaagat tttcatctttaaagttacaaatgtgatttctcagctgcagtatagaatatatggaaccag aaatactcaaaaaataattgttttcgaagtattaatctttttagtcacagttgttttttt acatcctgtgctgaaaataacaagaaatttgatacagagggaaaaatgatttattatgac ttagtagacaatttacaaatgtgtgttaagatattaggatatatgtcctaccagtttcaa aagtactacaagtgacttgttcaatacagagtacaactttaaaaaatgtatttaaagtaa ttgacgttgtgtttgtgacctggctcgaagttcttttttggtgaagagcatgcttagtac atgattgtctttaaaaagatctgataattatttactcagtaaatgttttgagtgccttat gagtcaggcatttttgtgggtgctggagataatagtgatgaaaaacaaaagtcccttttc cttatagaacttaaattctagtgtgggaaggcagatagtacagaaataaattgatggatg gatagatggataggtaggtatttgctatatagcaggtactatttttgcttttatatagtg ctgtggaaatgagtaaagtcaggacaaaggaaaagagaaggtgatgctggggggtattat tttaagttggatggttagggaaagtatcattgaataggggacatttgaacagagtcctct atgaaggaagtaaaagaaataaaaagaggaatagctagcacaaagactctgaggttgcag cacactttacccctcaagaaagaacggtgacagaaagggtggaacaaagtgagagagtag taaaatatgagttcacagtggtggggatgtatgtagatgatagaaggacccaacaggcaa ccataaaagacattggcttttactctgagtgaaatgggaagctcttagtgggtgttgagc agtggagtaataagataaaacttatttcatcatgcatttcaggtacttactcctaatcat agtaattaataaattattaatttggggtatagaaagttcatatatgaagtggagggtgtt ggctcttttaagaattcagtgaaaatcctaaacttcttgcagaaagtgattccaagcttt gagttattcttcctgatgatcagtacagttggtttactttcttgttatctttgtcctaaa gcttcttaaatcactttgtagctcaagcctaatggattatacctgcccatgtaaattctg aaaatgttaatgttgcctagtgattcagaggctttagtaatttacttaaactacttcttg ttttattggtataaactgtattcctcagtgtctactatgatttcaaagttagtatttgcc ttggaatttttctttgaagctggcaactctagttcaatataagacaggctctcagagctc cacttatttaacaactgtatctatgcccacttttattcctctaagcattcctagaataag catttcaaatggttttgaattatcaaaggtctttttgaaaaacaaggttattgagaaaat gtttaaaaagttcatgagaaagttgtctaaaatacacccccctcccccaaggtaggcctg aaatgccactaaatcaacaaaggatagtcactaaaagttaccctgtattttctaggactt ggggaaagtcatggagtagctgtgctttctctaagtaacctattttaaatttttttgaag agcaaaattattatacctctttttcatccagaatggagctgactgtaggcatctttttaa agtacccataggtcaggacctctctggactttccactaccttgaaagacactgccagaag aatgttgttccccacacttaaaaaaggaaaagtaatgttcttttctccagccattatttt atgcaaagtccaactttattttatctgagctatctgaactttcaatttatttgaaacatt tattgattaatatctataatggagcttttaatttctctgatcatttatcctttgttaggt tagcagaactgtaacaacacagtcactttcacagccacgaatgatacttttatagtcatt tgtggacatatactgtgcagagtatcacaaagtttgagccacctgacaaacttatttcca gttgagatctgcagggtgatgctctgccttcttgttgcagctctcataccatgaacaagt gtcattttcatggtccatgaggaaaaagcattttcctcattttggtgctttttgttagtg attgtgcagtttagaatggcccccaaacatagcctgaagttctgtttaatgttccgaagt gcaggaaggctgtaatttgccttaaagataaactaatgtgtgttaaagaaacttaattca ggcattagttataatgctgttgaccatgagttcgatgctaatgaatcaatgtatattaaa gatatatttaggcagaaacacgcataaaaaaggttatgcattgatcagttggcaacagtg ttgtgaccagaggcttgtgagaactgtatttcccctaggagcaatggctcagtatttgtt aattcagtggttgcagcaattttatagaacacaattactgtgaatgggaatcaactgtat ttgattaacggtggtgatttatagggactttagataccagaaatgtttcataattccctt tctctgaaatcactgttttaatatgtttaaatgcatgcatgcagagttgtgatgttaaac ataattttactgtgggttctggtcaaaaaagatgaaatgtcattggttaacttgaaagag acattattctaaacattatcctccagaaaattgacatgtacaggaagcatattgaatgct cagcaaataatttgttttagagtaataatctttgagtattgtagtgattcgagttttgga aaagaaggcagtatcagtgttagagaataatattctttcaggattttatgtagttacttt aaggtgcatgaaaggagactcagttgggtgttaaattcttgtccctgctttacttgaaac tagtaatcttgagaaatttactaaatcattttgagccttgattaccttgcctgcaaagtg aggaacattttcaatttaaaattctatttcttttattagtattcttcctgaccttgaatc atttctattcccatcatgtccagttgccaagaaaaccatgcaacttaatgttgtttgtta gccatcagattttattttactgaactcaagttcagtcacatcagttaccgataaaataat aagttctatattaatatttgatatatcttgacattttgtattatggtaggaagaaataaa agaccttcctcaaattttaggatatgggcttctgtttgttgatgtttgtgttttatgata cactgttgtaaatcatactaaaggttttctctgaaaaagactagaattgacaatacttca tttgttagcagtttcaagttttagatcacgggtgcataatttcttgtgtgtgcacaacat gttggcattttatctgacttcaaagatgtccatatagataccagcaaaggataaaatgta agaatgaatatcatgaaaagatgctgtatcagataaaattctagagcctaggttaaaaaa cataaaggcaaacatgcttgatatataaaaattgagaaatatgataaaaagaagtaagtt taaagcatcagtaataccactgcctaagatgcagtttattttgaaatgttgtcttctagt cttcatacagtttaaaaatcatactataatcacaatttccagcattttccccttagcata atatattataacatccatgtttttaaaaatattttatgaacgtcatgtttagggattaca ccatatatatcccatctcatagattcctttatattgaacatttggatttttatttacatt tttatttacatttatttttatctttattttttgctttcgtggataaactgcagtgagcat cctctgtataaattttttttttctgttttaggattacttccttaggatagattgccagaa gtggagttactgggtcagagggtatgaacttatgaactttttttttaaataaattttttg ttcattttctgtccagtcatttgtaaaaaaattaaaataataatgattataattgttacc tgctagaaaactgtgtctgaaggcaagattctgtgatcagaaaagtttgggaaataccca gggtaaaaagccttttaatctgatttacctcagcatttcccaaattaactgaacactgaa aattttttttgatacacttcttaaaattttgtggaagttttatataggatatagtctatg aactactgatgtaaaattaaaattgttttatttgcttgaaatattatgttttattggtca aaggaattaaactataaatcttcatgctaaaaattattaaaagggattagtgttgtttac tatggtttttgtgcatgtctttataaagtcttataacttttaaaattgaaatgttctata ttgtttactaaagaacattttaatgccttggtggcatcaatacttttttgcaatcattaa tgatatttagaaagtagacatataacatgaaagtagaacataatatagattgtacaaatc ttgttttttaccctattttcccctgcagaatgtgaaaacctttgcatcttctgatagtct agccaaggtccaagaagtagcaagctggcttttggaaatgaatcaggaactgctctctgt gggcagcaaaagacgacgaactggaggctctctgagaggtaacccttcctcaagccaggt agatgaagaacagatgaatcgtgtggtagaggaggaacagcaacagcaactcagacaaca agaggaggagcacactgcaaggaatggtgaagttgttggagtagaacctagacctggagg ccaaaatgattcccagcaaggacagttggaagaaaacaataatagatttatttcggtaga tgaggactcctcaggaaaccaagaagaacaagaggaagatgaagaacatgctggtgaaca agatgaggaggatgaggaggaggaggagatggaccaggagagtgacgattttgatcagtc tgatgatagtagcagagaagatgaacatacacatactaacagtgtcacgaactccagtag tattgtggacctgcccgttcaccaactctcctccccattctatacaaaaacaacaaaagt gagtatattcaatatattgttaacctgagaaactttacatatctattttaattgtaatga aactttcctcacagtctttgtattactaaaaattaatcttaagatgtgtaataataaaat gaactagttttttgtcttacagaaggtaatggaaagtattacaagtagaaatactgataa tgggacaatctgagtacaaatggagggaaagtaattgagcctattactatgaaaaattat taacatcaaattttcgtctaaaccttgcgtcccgggcagggattagtagcttgtgtgggg tgtagaggtttcgggtgaaacatgatttgtgtaaatcaagtaatgaagaaactttttatt tacaaaatgcagctgctagagtgggctattcacaagtgtcactctaaggcataaattact cagaagttgcctcaaattagttctagttatttatcatttgagaattagaaataatgaata ttcatgtagagtagacttacaagttaaactatgttttggaaaggaacattttaacttgaa tatacttgttttatagacattaacataatgtctgtggtagttcatcctcttactcctact tgcttatttcttacattttacagtgattttaggccataaacaatatacaaactgatttaa catcgtatcgtttaacctaacttgacttggaatgaagtatcttacacacttggattaata ataattgaaatgctaaatcatgttttacatctgcttgtaaaaccttactttctaaaatgt gtttggccctaatttacctagcctatctgcaagtcagattttatttagctggcatgatct aagaaggacgtgacatcaagtagtgatttttcatccattgtaccatccctgtctcaagat gttgaagcagtagctttggatctcagccagatggctctaccactaacaactatagcacca ctttgttaaggctcagaccttttatagtgtcattcctcccttttgcccccctagtcttga tcatcttcttcaaggttgctatatcaaaagggagttttaacatctctttttttcctgagg gttcagttagaggaataggggtttaatacttaaggtccaattgcactaacttatttcaga aagaggttcaatcaaaactaagccttgcggaacaatgcagagttattgctgatttctgct acactttaaattaaaagcaaaaaaagctagttagacaaaacattatctttttccaaaagg aaaagtcatccttatattctgtaggaaaccttccagttcacaagtttgacaggtgcttta tcttttctttaccttcaagatgtaaaattttgtgtatgatgattacctttttaaaaacga agtatactgaatttgttagtttttttctagtgattctgaatcatgaataacaattattgt cctgtacatataacaagaatggactgggctctaaccatagaaggaatacatgtgtgtatg catttatgtatatacacatgtacagtatagatgtgtgtatgcatttatgtatatacacat gtacagtatagatgtgtgtatgcatttattatataagtgtgtatgtatagtttttgatat attgttgatcatgagatgatctgccttcgtaagtatggcatgaagaatacaaagctattt gattgccaggacagtgatacccttctacaagtaagatttcagtctgaaatttttctaaat gttttcctttttgtccattcctgaaacccttctgccagagtattcccagagcactttcaa cagcattaaaaaaagtcactgagtactgcttcacctaaaaagaaaatgtcagtattctcc gtgtatacatgttttaatacaataaatagaagctctcactagttgaagtactttattttg gaagagctttaaattcttgatcattataaagttaagacacttctacagatgttaccctag accatagtttcattcaagtcttctttgacttttgagagtagaggcataagaaatgaaatc ccattttaaatgttaacataggattagctttctacaaattttactcaggatacctttcaa gttttattttttggcattctcataaacaaaccctgtggacagctagactgaattttaccc atcatccctaaatatgctgcataagcaaatatattaatgagaaaaaaagatttttaattc ttccttgcattgctttattctttgaaagcattctgaaaactgtcaacactctgaggacag tttttccccccttgtcactaaaggtttgttcttttgggagccttatggcaagcaatatat gttaaaaattccatgttgtcttaatgttaaaagtcaagggaagtggtagtcaagaattgc tatttattaagctcacactgtttgaaagatcctactacagatacataaacaaaagttaca gagaggccgaatgatccaccccagattacactacgtgatgagagattttaatcctgattt gcctaatagcaaaaaacccctttctctttccacatgctatgctgccccctatggttgaat ttgacaaacatcactgaacaagatttccaacatagtcagagactctgccatgtgatgatt ttctgatactatgtcccatttgaagtgataagagctaccctatagaaaggacagagggct cagttaactacttttctttgtggcacttaacaaaacattattgaagattttacagctcaa ggctaaagtaatcaggaacaaatgacgctgttaaaaaattttatagaggtcatactctct tgaatccataaaacatgcttttaaagcagaggttgtgtgctggctattcctctaaatctt gcttctccagatacaaatgtctgtctcttcagttttaactctcattctcaacatccttat catatttttttaaaaaaagttgttttctgaagatatgcccagctcatattgaagaatctt tgacatattttatgcagaatatcaactaattttctactaacatgaaaaacaaatgagatg attattaggccagatctggtatcctgatcttttgatgaagagaagtgtgtttgagcatta atttctgatgcttccatatctatcttagtggcttccacctgttctcttctcacataactt ccttacctcaaattagtctatttttttcccatcttgattgttagattcttcctagtaatt aaatcgtttttatccatttgcctcaaataagaaataaatgtttatcaaaaaataaatata tataaaagatagccaggtgtggtggctcacacctgtgatcccagcactttgagaggctga ggtgggcagattgcttgagaccagcctgggcaacatggcaaaaccctgtctctacaaaaa atacaaaaattagctgagtgtgatggcatgcgcctgtggttccagctactttgggggctg aggttggaggatcatttgggcccaggaggttgaggctgcagtgatctgggccacagagca agaccctgtttcagaataaataaatagagggtggggggaggtgtggagaagcatctagtg tattggagtttagaggcgtaagaccctttcttgtctataaattctgaacaatagaggaat caggtaataggatcattaaccaatcagggtatctggtccgccattctccaaccacctcct aggaccagagctgctgagcagctttgtaggataatgtggctaacattggaaatataacag ggctctaagttacactctgttgtagagttctagagaatgatagcttcttcagaaagtccc tggcctagtctacctgcaagacaagcaacttaagttcaaagtctgtttggtatatacatg tccagagagcaaaaaatgctatttaaggcagtcatcttgaataacatccactagtaaaca tggcccaatgaatagccatgtcccctacctttgtgaaggaggaaaaatgaaacattgact cagcctaatttagcccaattttaaacatgtggggaggataatggaaatgggagaaatggg gacaatgggtgagatgggttttcccctaacatattagagtgataagaacttttcacatct gagtttgaattctgattataccacttactgtataaccatgagtatataatctcagtgaac ctattactttatctgtaaaataaagactcatgcctacctctcaaggtttttgtaaggatt aagtgaaataatttatgtaaaacaagtagcatagtgcctgggacactcagatattttgca gatatcggccttgtgacctcattttttccgtctcctgtgccttaccccaaatcccacaaa ataaacttcattccccgcagcggttgtcaatctctatatatatgttttacaaccttcagc attcagtcatcatatcaccctctagtgtaaaaagtagagaaataaattcttttaaaaagg tacataggaggattagaagagtactgtaataagctaggcccatcttcaataaaaatagac ataaattttgaagtcgatgctacccacagatctccctagttgataaaatatgtacccttt cctttcattagaatttaagtcaatttctctagactttcttctaatctttaactaaagctt ctgtatatttaaatgctttaatgaatcttccatatggatgccaaaatgaattttagctca tggttaatttctaattaatgcctgttaatatagttttaaatgtatttgttaatattattt taaacaataatagctctaaatgttggtgataaactgtatctcattcactgcaggtaatga cagttactattattaattatttaagagacggggccttgctgtgtttcccaggctgtcctt gaacccctgggcataagagatcctcctgtgtagctgggactacaggcgtggaccaccgtg cccagctcaaaaagtattttaattatgaggtttcattgtagactaaagaataatcttcac accaaatggaccattttaaaattgttttatagcattccattgattagttctttatacaga tgataaacttcactgaagtatacacaaatatcacaaatagttatcttcaaaatgcttcca aagaaaattgtttcatatgtgtattataatgatctgatccatttttaatgttgtttttgt tggtggtagggaagacagtatgaagaaaaaggacataggaatagatcatgatgaatattg ttttcactttggtagttaactccaaaattatttctcaagctccgatcattcaccttagcc atagttttgcatttctagctgtctgttgggcatcatcatctgagtggtccactgaaaact aaaagtactgtttctttctccaaaccaagtttttatttgcctccccaggttatcttttcc ctctcaataggtcccatcttctggtcagtggatagaaaagtggcttgactttttcttatc atctgagtctgggcctgctgaataatttaacagacttgatcacttagtctctctttttct tacccgccaggcaattgtaagataaaaattggagggtcagtgtttaaaaacgttaagagc atttttgcttgggcttctgtttgcatcaggctataacgccacgtccttgacaatgagttg gcagtatgaataattggatgagttttttaaaatgaatttttagctctgtatagataataa cctcaatacagatatctggtgccccatttcccactgataaagccaaacctaaatgagaat gtttgaagtcttccaatatcttaccatagtttttacctggtttcatctcctctcctctct acttgttttgaatcatactccttcccaccagccatatccttcaaatcgaagtccaaatcc cctatcttaataccttcctagatttagaaaatcgaattcctttcaacctgttcttgcttc tcttcatggtgcagttcatttggtattacctggtgacatttttggtaagctttgtcttaa gttgacatataattcttcacttttttgagcagtagaaatttggtaaatttgttttcagtc ctaattcagtttgagaattgaagactggcaaactgtaatgtgaacagttgtcatttcacc tctttttttttttttttttttttttaagacaaggtcttgctttgttgcccaggctggatg gagtgcagtggcataatcatggctcactgcagcctccacctcccaggcttaagcaattct cccacctcagcctcccgagtaactgggaccactcgtggcaccaccacgcttggctaattt ttgaattatctgtaaagacaaggtcttactatgttgcccaccctggtctcaaactcctga gctctggtgatcttcctgcctgggcctcccaaagtgctgggattacaggtatgagccacc atgccccagcctcatttcacctcttaagatgtacctaagctagactagaaattgtaaatg ggcacgtcattaaaattgagtttaagactcaggtattcagtatatgtacagatcgttaaa ttgagactgccctgaggagaagttaaaaatcccactttaggaatcattttgctaacatgc agaaatcagttgaaggcaattcatacagccattaaacatcacatttgaaagaatatttaa taacaggaaatgcttaaaaatattttatttaaaatataggattcaaaactctatacagtg tgatcttaattatgaagcaaacatacatttttatatatgtgtgtgtgtatgtatctacat atatacatatgcaaagaaaaagatcagaagaagttgtaccaaatgttaggaattatctct gaatgtggttgaataaaaggttattttttcttaaatacattttataatttctccattttg ttataacaagcatatatgcctctttatttgaaaaataactctctgctggaaaccaattat tattctatgtggtcttttttgatgactttacatggtgtataatgctcctaatggaggatt ggataaaaagatgttcctcacaggaaatgtcattatatcatgatctctgcatgagaatgc agctgaatatagtggaaaccttcccctattcccaaccccccatttcatagtggaaagagc acagaactctttaagtcctagtgtaactgattcttcacatctctagggctcaggtgttac cctggcaattttctaagatcaacttaacacgttattagaagagtaatagcatagtagtat ccgttcttgttttgaagatatttattgttttctttgaagagttctttagccttgatagca cttttggctctctctaaacatgaatatttttcatataaaaaccgttctccgagcaatttc agagtctgtttttgaaaatgggtgtccccaacccccaggccatggaccagtctgtggcct gttaggaactgggcctcacagcaggaggcgggcggtaggtgagtgagcattaccgcctgt gctccgcctcctgtcagatcagctgtggcattagattctaataggagcacgaaccctatt gtgaactgtgcttgcgagagatctaggttgtatgctccttccgagagtctaatgcgtggt gagctgaggtggaacagtttcatcccaaaccatccccccacttcccctgtccatggaaaa attgtctttcatgaaacctgtctctggtgccaaaaaggttggtgactaccgttttaaaac acaattcttttttttttttttttgagatgaagtctggctctgttgcccaggctggagtgc agtggtgcaatctcagctcactgcagcctccacctcccaggttcaagcgattatcctgcc tcagcctcctgagtagctgggattacaggtgtttgccaccacgtccagctaatttttgta tttttagtagagacagggtttcaccatgttggccaggctggcctcgaactcctgacctca agtgatccacctgccttggcctctcaaagtgctgggattacaggcatgagccatcgtgcc cagccttaaaacataattctaaagtggccactgctttatgaggaaatcatcagtgctttc ccttccctcccttcccgtattcccaactccccatttcatcaaactaatgatatatttaac atgttaagggtttttagtcctgattttaagtctcctaagacagaccccaaattggagtgg acctttgagatcaccaaggccaaacctgtgcccagtggctttaccaacagacttgaactt acaaatgaggtcattacaaaggaatactgcaatgtgaagtatcttcatagttttagacgt tatttatgttagggttctttattttgtgttgacatctctgactttgctttggtccttgtt cttctacctagagcagtgacagctcttttcataggagaagattgctgccatattcatctg tgatcttctccagactgaatgccttcaatgtgttcccagaatgtgtatatatacttatat tttttctcatttatcttaacagtcctaatatgtcatttctaatctttaaccccataatgg agatgagaaatatgtgaagagaggttaagtaattaatcttgtatcagtcagctggtaaat agtggagttgggattggaactcagatcttatgctgggtcccggttgtacttttgttaacg tttggaaattcagttaaacaatattagtaattttgggatgagtgggctgtgatgaaataa cctataaagctaccgccctaacatttattttcatgtccccaaatgttgcctcaactaaat ctctgttactgtggtatatttattgtgactcatcgtattaagtttgtttttctccctctc tcttaagccctttgatttcttattttattgcctaataatatttattgtgttatcaggtac cctgataacctaattattagtcaacaaagaattagtgtataaatttttacataatacttg tcagtcattgaccacatggatatgtattttgtgtagctggagtcagtgtgtatacttctt ttatattcctgattctttcagttattatgataataatagtcttattattatttggtaacc tgtttgaacagttgacttgttcagcccctcaaagggaatttcccccactcccctctgctt cactgaaacatgacccattaacatgaattatttctacattccttcacctagtatttcata tgtatcttttttcttctatccctccaatctggtaagaagaaatacacttgtcctttacaa ggaaaccttgtctttgaattattgatccttgaggtctgcgaaatgttttttggagacttc ctgcattagccattctgtctttttaaattaacaagctttaatttttagattagttttaga ttcacagcaaagttgaatgagaggcctagagagttcccatatgccccctggtgcacacat gtacagcttcacccttaccagagtagtacatttgttatagtcagtgaatctacctttgtc atatcattatccagaatccatagtttccaccaaggtctcactcttggtgttgtgcactct gtgggtgtgggcaaatgtgtattgacacgcatctaccaatgtagtaccatagagaatggt ttcactaccttaaaagtcctgtgttctgcctattcatccctttcttccccagcctctcat cgccactgatcctttcaccgtctccatagttttgctttttccagaatgtcatatagttgg aattatacagtatgtagcctttcagattggcttctttcacttacgtaatatgcattgact tttccttcatgtatatttatagcttgatagctcatttccttctagcactgaataatattc cattgtttggatataccacagtttatccacttccctagtgaaagacatcttggttgcttc cacataattggcaattatgaatatagctgcttgtgaaaatctgtgtgcaggattttgcat ggactaagtttttaactcctttgcgtaaatgccaaggagtgtgattccaggattgcaagt taacagtatgattaattttgtaagaaactgccaaatggcttccaaagtgtctatatactt ttgcattcccaccaacagtgaatgagagttcctgtggctccacatcttcagtggcatata gtgttgtcagtgttctggattttgaccattctgataggtgtgtatggtatctctttgtta ccatttgaagttccctagtgatatatgatgttgaccatcttttcctgtgcttacttccca tctgtaaatctcctttggtgaggtgtctgtccagatcttttgtccatgttttagtcaggt tgttctgttactgttgtatattttggataacaatcgtttatcccatgtgcattttgcaaa tactttcttccagtctgtggcttatgttcttattttcctgacagtgtctttcagagagct gacattcctattttagtagagtccagcttatgaattatttctttcatgtatcatgctgtt agtattgtattgttgtatctaaaatgtcatttccggctgggtgccatggctcacacctat aatcccagcactttggcaggctgaggtgggaggatcgcttgagcccaggagtttgagacc agcctgggcaacatactgagacctcctccttaaaaaaaaaaaatgcacaaaattagccag gcatggtggtgcgttcctgtgatcttagcaacccgagaggctgaggttggaggattgctt gagcctgggacatcaaggatgtggtgagccaagatcataccactctaccccagcaaaaag tcatgtctatacccaaggccatttaggttttctcttatgttatcttctaagagttttata gttctgcactttacatttatgtctatgatccattttgagttaattttgtgaaggttgtaa ggcctttgtttagattcatttatttgcatgtggatgttcggtggttcagcaccatttgcg gaaaagactagcttttctacattgtactgcctttgttccattgtcaaagatcactgtacc atattctggtctctctattctgttccattcatctacttttaattctttcaccaataccac actgtcttggttactgtagctttatagtaagccttgaagttggagagtgtctatctccca gcttcattcctctccttcagtatcgttttggctattctgggtcttttgcctctccatata agttttagaatcagttcgttgatatctgttacttgctgggattttgattggtattgcttt gaaactatagatgagattggcaaggactgacatcttgacagtattgagtcttcctgtgca agaacatggaatatatctccatttatttagttcttctttgattttttatcagagttttat agttttcattatgtagatcttatacatattttattagattcatccctaaatattttattt cagggggctgctaatgtaaatgatactgtatttgtcatttcagatttcactttttcattg ctggtgtgtaggaaaataattgacttattatatattaatcttatatcttgcaaccttgct ataattgcttttttttggtcaattttttaggattttctatgtagagggtcatgtcatgtg ccaaaaaagacagtgttatttcctctttcttaatctctttatcttttacttccttttctt gtctttgtgctttaactaggacttcccctatgatgttgaaaagcagtggttaaagaggac attcttgccttgtgtctgatcttagtaggacagctttgagtttccatccttaagtgtgat gttagctgtaggctttttataggtagtctttatcaagttgagaaagttcctcccctctat tcctagtttgctaagagtccttctatcctatttcttaatggtttagtagatgactctgtg gtactttgaaggttgtttgcagaatttccatgccataggcaatttacctttccttgacat ttgaaggattgatgttggtgccaagtatagaatcttcacagagtcctcctgtagcttcta aaggtttagcttgaaaatgttaattgcttaacgctagtaagtgagtgaaaaagctgggga taaattttgtatcttgcttatatttcagttcccacctctgtcctgacaaaaccccaatat ataaacagtaaatgtaagatatatatattttaaacataataacaaatgtaagttcctttt gaaattattatttcaccaactttaaacagtattatcaatttttcaaacatttggccagtg gttacaattttatcaatcttattagtgagattcttttctgcttgtgggtctacttttact ttgactcttgtcctgaaatctcaattctatctttggcctctcttctgagagacataattt tgtgtatttaattaatctctattttctataaattaatatgataaaaagaatcaagttgga tggatcagaagtagaatacagagaaaagaaaatatggttagggaattgtgattaggtatg tttaaatacttagttcgtagacacgaccccattcttttgttttttctttgaaatttgcct tagattcagtattgtctagttcttaaaatgctattttttttcatttagcattacaattat ttgtgggaaaaattttaagaacatttcagaattctttcactggatattctaatatggata gtaattcaaaagaaagcacaaagatgtaaaatcacaaaagatataaaatatttaggtttt tgtaaaaatcatatattagtatgtttagcatacatagaaaattttataaagtattatttt tttttttactgtgaaacatcatatcaaaagcacacaattccttaagaatttatctgtttc ttcactgaacactagagggccatatagcaccttatagatagagcggctttccatatattt aaatatatttaatatgtgcttgtctttctctcctactttgtgttttagtatagtatgtct tgctcagaatttggagtatacactcaacagtaattatgtgcactgtcagataatatgtgg gatacctagggtttttgtttttgctttttttttaacaagcagctagcattttgctactta gtaacaacaaaaagagataaagagtgtatcttgggataaatgtatgtgcagacttactat gctttggaagtataagtatgcttttagaaaatttctcaagtatctgaacatatacgtgta aaaagaataggcctctcattgttatttgctctggtacttcttggcatgtatgtgagaaaa gaattatctctccaaagaagctacttcccttgtctaaatgtcatcttgtattagcacaga cagtaatattgtggggtataaccacctaatcatcgtggagtaaagaatatttaaacaact gatttaatcttcctcaattttttttttttttttttttttttttttggcttcttagggtaa agaaatgagaaatacaacttacaggaacttaaataggtaatgtcaatgtgtgaagagagt catgaacagccagtaagactgttcaggctcttatgaactacgtaagatatgccttcccca aaaggtgttaatgttcagttaaaagaataaaaatatgaggcaagaggatggtttgagccc aggagtttgaggctgcagtgatagtactacagtactctagcctcggtgacagagtgagac tctgtctttagaaatatcaagaaaagaaaaagaaaaacattgtgctggctccaaaaaact gactttgagctacatatgacctatgggctgtatactgttggatctttaatagcttctaga gaagaagttcaagatctaagtagggccttggctaaattttaatttcttccttagatatac tttagacagcacttttcattattaatttctgtgttatgtttcagggggcagaaataagga catttctcgttaactactgtttacactgtcttttggtgaaggaagattagtatgatgaac ttagaggaaacacactgtttacacctagaggagaaaataaaagagggtcatgtgaaagag aaagaaggaacaataattcactcttaaagacttaaaagaatctcatcttttaagtctctc ctcctttgatttgccaattttaataccttaaattatttcttcaaattagttactataatg gagtgacaaaactttgatctttagttctaaattttaccgagatgttagtcctcttgaata ttgatgaacttacaattattttaaagtcactgctatgataaacagtattctctaactgat aatattttttatgccttagtggtgtgtttgctagttttctgtatcactgccattttctga aaaccacatttattggcagattgacatgagcttaggtaattgaaggctgagcttgaattc tgatccttttgcacaggtaatgaaatgagttattgttagcataatatagctggtgcatgt tgaactcttaaatggtcaagaaggtaaatcaaataaaaagatcagtatttggatatgttg aaaaacacatggactccttctttgagtggcagagataatagatgctattgatggattgaa gacatccattattgtgtctttaacacgtgtctggagaaaacactggcttgctagttaaaa cttgattcatttaacagtattgctgagcactcaaagaattgccattatttttctaagata tgccacaatttaatagtagctcttgtagggaggggttggggaaggacgatgttgggagta gattactatgctaaatgcatatattcattgttagttgcattacattttaaaagggttaat gggagggggcaagtgcagaattgaggatatgtattattttataaagggtaagagatcact tttatccacccagaagtcagaggtttggaaaaagcaggttagtatgaatatgaatactag agatgtttctagaatatcatacacctgtattgtaactaatttccatcaatgatttattta ctaacctggaatgattttcaagcagaaaaccctctggaccatagggtccagaaagttaat tgatatgaatgaaatggactaaatataagagccagggagctaaaacttctctaaaaagag ggtcttgatattgccatgtttttgaatttttcaaaattcattggaatttcagattttcag atgatatttcttaatctttaaaatatattggtgtccagattttgcaaaagcacttccgtg ggttggtttgacccatagaccacctatttttcactagacatcatgtagggaagaagaatg gactattattcttgaactgaataactgaaactgaaataaatgcaagggaagatgttcttt ggcacttctgtagaaatgtttatcacccattgcaaaattacgatagtttgcctccagtgc tgatccattaatgggaattgggttgtttggagtggaagtgcggcatatgcatagcacttt aagtgctttagccagtgacctacggacttttccttatgagtttggtgtactgattttagt tattaaactgccaaatgaattgacctcattttaaagaaatgagtatccaacttccacaag gaatttgagatttgaagtggtctatatgtaggaaaaatacatataataaagtgacaaagt gtacatgaaaatcagaaccagggacaatactggagtagaatatgagggcaaggtcatgag aagaggtaaggattgagttatgatgagttggagaaagctagtaataggatataggttgtc cactcttaagtgtctgcaccagtatttttcaaagtgttaccttggaccagctgcatcaga agcatctggagtgctttttaaaaataacagatttctgggttactctcaagagctacttaa ctaaactccagggtcgggatgtgcatttttaataagttctcaactgattttcatgtaaac tgaagtttgcaaaacactggtgtgtagtttgttgtctctgctgccctgtgataaaattga cgatgtactgagtcctcaggggaagagaaaatatgtcacacacacacacttatgcacacc catgcatctttcctaatattacataaaaggaatttcccacataggacttcctgagtgact cagtagatcatgtcctcaacagtatagctataacaaatgcattaattctcatgaatagtg gtacagtgtgatctctaaggtcaaactacggggggattttcaaatgctagctctgtcact tcatggcagtttgaccttgcacaaaccgttaaccttgtagagtttcttagggacggtagt agcatctaccacatagtgttattgagaggattcagtgaggtaatgtatgcaaagtactca ggacaatgactggcatatagcaaatccttaatgagcatttgctatggtatattataaggt taattattgtaatgttacaagtctatgctataactaactcactagttcaggctgggagga atgctatgcgaaatatttttagttccaaagatgctcttttgggtaggttgaaacaattcc attgacttttgcctacattattaatggcttttagcatatagttcagctgtttgatattgc acattttgttactgctaactatgaagcactcttgatccccgacagatattttttccctaa ttggttgttaatgctgaacttagaaggaatttgtaaaagaacctacaagtcaattttggt aatcttagagctttgagtactcatatgaaaatgttcagaagagaggaaaaggcaagtttt attctttaatgatacttgggtgttcttgaactgaatagctgaaactggaataaatgcaag ggaagatgttctttggcacttctgtagaaattctagaattactgcttgatactacagaaa atatctaagtttttcacagtgaagagatgattataaacaaatgaagcaatcactatgaat ggaacaatagttttgggtaggttaaaattatctttaggatacattttagagaacacttcg taaattattagggtaacttctagctgtatctgacataggatattaacttcattccttgta ataagtattgtcagggtagcatcttgcatataaactttaacctctaattatatgaaatgc aggaagaatgatgattgttttcatagatcaggtttaaaaatgatgtttaaaatttttttg tatctttccccctccccagaaaagggtctgttttattcaaacgtaccttgtccttctgtc agttggtttttgctgatgcaaaaggtagtttctctgctttagaaatagcagttgctttta ggagatgcattacttggttggtttccatggcagtttttggttattgaaatcagatgcaaa tgaggaatggaagtatgaagaaggtactggtgaatttgaggagtaagtgccaacagttat tcatttgcttagctacagatgttggttctattaatataagactacattataagtttaatt ttctagaatgaattgtttgtactttaccgatatgctgccatctgtcttagggtttaatga gtgaaaaacttaaatatactgaggtacctctgtagtgtttatcacgtgtttcagttttgg ggtttattcatcattatcattttactctgttacttggtatagaaaagaaaattaaaactt taagacagaatcattattctctatcagttagattatcactaatcttacattttattttaa ttatatttttgtgcttctttactcagaagggaacagaatagagattacaaaatggtgcct tgtaggtatttaaatctttttcttttcatcatgacagcttaccattgaatgtatttttaa aacacatttaaatttgttgccaacattggaaaatcttgagagttcacataaaaatattga tttttttcaccttcttttgaaaatatggaacaaatggctttgctgggcacatgtacttac atggcaacatcaactgcaccttctaaaagggcttttcagatcttaaacctaatttgtttt tctcctttattatacttgccttatttgtcatctgaatttgcttggaatatagacaaaagc atgattttaggaaatccaaaagtgagtttgatttttcctttcttggtcataacatcaaat gactttagatgtaaattcaagccaaagtaactctttaaaaagttttattatgaaaataga aacttgctaaggcctcatttccattgagtgtcaaggtgggaaattgtcacccaaaatttt gccatcctcaagatgatcacttaacgttttcattttgaatcacctgttgtcctttccatt cctgttacattgtaaaattaacactccactaggatgactataaaccaacttagagctcct tgttttataagatgtctcggttatccattgtcacctgaccaatcaccgtcaaagctttgt ggattataacaaacgagaattttattatttctcattgttcttataggtttgtaatttgag gagggctgatctggccattgttaactctgggtggcagtcagatcatggctggaggtgcaa aagtgggggtctagaacagctgagggctgacccagaaattctttttccgtatcatctcct aacatctctatataatacggtctttttacctgggctagtttgggcttcttcagagcatgg tggcctgaaggtactacttagccagcacatcaggactaccaagtgagtgttccagcaagc aaggtggaagctgaatctcctttttatgactcagcctgtgaagtcacatgataccatttt tactatctctaatggtccaaacagtcacaaaggtctggcctgttattaaggcaagaggac atggcctctccctcttagtgaaaggagtctcagagtcaccttgcaagaagagcatgcaag atgggggacagcgttgtgactacctttgagaatacaatttgctataagagttcataatat ctttgatgttattcctgtttctttttttaaatttgggcttcataaaaatcaggcacatta aaacattccatatttaatatgtaaatgtcttatacaatactttatttcagttatctagaa ctcatttatatctgacaaacttacatatctgaaacactgacaaagaagtgtctccgaaca actaatttaggaagtaacacaactaagttaggatgtaacattctgtggaattggatagat gttctgttacaaagcacaggtttaggaattagacataccagggttaaaatctcaactcta ctacctggcagctgtgttaccttgggcaaatttgtaagcctttctgagccttagttttgt ctgtaacaagtagacaaggataatgatacctcagggtttcggcgtggtgattaagtgata caaagcatgatctagtagaaactcttgtcagtgtttaatctcttccccttatcattgttc cagtctgaaacaatgcgtacgtattttctaagccctatagctgcagacttcctttatatc tcttactgatctctgatagtctccttagacttatttatctttctatcccaagatctttgt attacaggcatactaaagaatagtaggaattgaattttatttctttgtttctaatttttc ttgatagccagttagtgttttacttactgtaaagaatgaaaacttaggctctcagcttaa tttaaacctcagctacaaaaaataacattgatatttttcagaaaaattatttttttgagt atgtaatattgacagcgtaaaaggccagtggtatcaactgtcagcccgtccaagaattaa gttctaaatatgtattttctattgcttaatcttatacttgttttctgtactttaattcta tacttaaattctgtgctgaagtactttcagttaatattaataaatctcacatacttgcat agcacattactgaaaattatataaatgaagtatggtactatgtttatctttctcaaaata tattgatttttatttataattgcatcagaaactcaacagtataggaaataatgtccccct cctattttacctatttaaagcaccagcactccttcagattttttatgtttccataagtgt agctttttatttttcataagataaatttttataatttgtcaaactgtcaatttggttgag ccttctgcattatagcttaattttttaaagctgtgtagcaaaaaaacacacaattactta ctgtattttaaaagaatattagtattatttccttagtcctaattattagtataattagta ttatgactccctggcctgcaatactcttaagaatttttataagagaaagatattattggt aagtaatgtttaatttattttaactgaaagtttaaactgttatgtttaaactgtttatgg acttaagtactgacattaaagaagttattgtctttcttagacttaaattttattttgaga attatggaaaattaggttactttaaatcacttactctgtcattacatgaggctttttgca tgactgaaatacctgtaattactgtgtcatttttgtcatagttgacttaaaggtgtaact ataataatcacgaactaaattcttggtgagcagctgtgtaaggccattttaacagctggg gtaacccagctcagcctgtaagagttaagcttaatcatgagacaggttatgttaagtcat tgaagacttcatactgggcatttctgaaatacttagaaaattcagtgggatagttggatt tttgttttatcttaagtactttttaaaatgcttaatgaaaggatgaggaaaagtatttta acaactaaagttcttaggcacaaataatagaaataaaatccaaagtttactcattaaaaa aaaaaagtcaaatatactctatatgctagtgtttagaccactacagtaggtaggagagga aaaaaatatataaacacacacacatattattatagtataatatggaatcagaaaaatcct gaggttatttttcttgaatttcatcttttaaaaatttttttaaaaatcattatgcctgta gcttctttccaataaaaatgttttcttattactatacaaaaacatttagagcacaggcat acatgccttttgtaaaagaaggcagacacaaaagaattgaaacaatgcattttatttgga tatattttataaactagctatattgttcactaaactataataaaggaaagctcaccaaat tgaaccttcttacaagaaggccatatatgtaatattattagagctgtaatatgctttttt ttggtataaatttaaggggtactctagttttttttacatggatatattacatagtggtga aatttgggcctttagtgtacctgtcacccaaatagtatacattgtacccgttaagtgatt tctcatctctcagcatcctccctgcatgtcacccttctgagtctcttaatatctattact acacactccatgttcatgtgtacactttacttagctttcacttgtaagtgaaacgtggta tttgactttctgtttctgtgttgtttcactgaagaatcacctccaattccatccatgttg ctgcaaaagacatttcattcttttttatagctgaatagtattccatttgtgtgtgtgtgt aaacatatatatatgtgggttagttatcatagttgacttaaagttataactattataatc accaactaaattcttgggcatctttaaaataatgatttcttttcttttgggtagatgccc agtagtgggattgctggatcaaatgatagttctattattagttctctgagaaatctccat aatgttttccatagagattgtactaatttgcattcccaccaacaccgtataagcatttct ttttctccacatcctcgccaacatctattattttttgtctttttaataatagccattctg aatggtgtgagatgagatctcattgtggttttactttgcatttctttgataatgatgttg aacattttttcatgcttgtaggcatgaaaaaatatgtatgtcttcttttgagaagtattt attcatgttcttttcccactttttaatgaagttaatttggtttttgttgttgagttgcct gtcaattctgcatatcagccccctgttagaagcatagtttgcaaatattttctcccattc tgtaggttgtcttttcattctgttgatttagagatgtaatgtactcttaaacctgcaagg gacttaatctcaatcatctgaagagaagtcagatagtctatatctattaaatatttagcc tcctctttgtagaaatctgaagtataaactccaggtaattgtactgcaagacattaaata gttaaggttcctgaatcttggagtttctggtgtaccatgggatggttttgtcatctgtca acttataatttattagcacttgttttggttggttttctgtgttttactttttaatttata atgtattttcagcaaaatttttggaaataactggaatataaaacagcttaaatgtctaac attgagatgttgattccataaatcagagcagttacaataccttatataatctagtattct ggcatttttattttgagaatgctacctatctgtgcatataggcatgtacatgcaatgctt ttattttgcaaatgtccaattatcaggtcacatttttataacacttgtgtatgttgtatg tgctgcttcagaacccaagcatatttctcttagttaggggccgcccttgttgcccaaatg aagaaaattagcagggaagtgcagtatgttgtccattgaatgtttacatacatgtaatgt ctcaaatacattataattggaagttgtaatctgagtgagcactttgagcatgtaaataaa tatcttttagaacatttttagtaatcattttaaatgtgattttaatcttataaaaacatt ttaatttatttgacataccttttgtgaatactaaagcttaaaaaaaaaatactaaaactt taaaagatatcctgaccaggcgctgtggctcacgcctgtaatcccagcactttgggaggc cgaggtgggcggatcacaaggtcaggagttcgagaccagcctggccaacatagtaaaacc ctgtctctactaaaaataacaaaaattagctgggcgtggtggtgggcacctgtaatccca gctgctcaggagactgaggcaggagaattgcttgaacctgggaggcggaggttgcagtga gccgacattacaccactgcactccagcctaggcaacagagcaagactcagtctcaaaaaa aaaaaaaaaaaaggtatgccatgttttttttctttgatgcccaatctaattgtatttatg tttctgcatttaaagtaatatgttgcttgctggatgtttttttttttttttttacagttt ttgctaatacaagcttgataatttatcattaagcagagggtttaatttattgatttatga ttgttttcctctgtgtttaaattgttccgtcttcaaccatgaattgcgttcatttgtgaa ttgcttggtagggaaatactttaatactctgatatctatgatgttaggaagactaaaaac tgatttggtagataaaataatctgctttactctgtatctgtgaaataagtgagtatgctt gataataccatattatctgatcagaaggattttgattctgatttattgcacttcagtaat ggtatactttttagcatgtgggactttctgtttctacaacagagtaagtcacttgttgcc tgaattcacagaagcagtgaataatactgaattcaaataacatacgttgatattattcct gtgaaagtttttgaggtaagactttgaaaactcttggtataaagtcaatttgaatttgca gggagatttacatcttctgagtgatttgttttagctgattcaggaataatttatgaaaaa tggaaaatctttgtgctgctcatatgctgtggctttggtcaggagcagttaaatttctag ccttttgtataatactgaatgaaagaaaagatggaaatccagagtaaattgctgtgatgg tatttttattgttgttacaagagaattggagtgcttggacttgtaatgcattttacagat accgtattggtgatattttattacatgtcacatttatatataattgtttttacggatgca aatttttagaagaaagaaaatcagggagtgataagtgggcaaaataagttaccttatata aagaccttttctttactacaagttccctaatgtttagcccatcttggatgatcagtggta tctggaatcttaaaatgccaagacactatggctaagatactacagcttcatattttggca aaaattttgacagaattatagaagtctttctgaaacttacaaataatatgttttaaaata ttcttttccaaagttattatagatggattttagaatctgctttatttcctggtgatttta aaattggaaatgtaatctacaaagctctatgtgatttttgattccattaatagtagtagt aatcataatgacagtttaattaacactatcgaacacttactagatgtgaggcgttactct gtatacttatatcaactaatttttaatcctcacaacagtcctatgagtactgcctaggtg aacttgagacacgaattaaattaaggtcatacctggtaaagccaggatttggactaagtt agtttggttctggagtccatgttcctacctatcatattgcactgccttattatttgtggt atcatctttaaaagagcttgtctgtttttaagagtctcaatttgggtagtaattaaagag tatctttaatgtttttaagagtagaattgagactcagactttatcttttcttgaacacat ggaattagctacattttgaagtattaacagttggggttcttatataccttgtctatgatt tccctcatttattagggtttttgtctatatttgaaggtttgtttttttagtgtttcgtgt tgattgggaatatcgcattagaaatactgttacttgaatcctcccaaaaaaatgaaactt gtagtatatatatttggaaattagtactcactttgcccaatcattgataatgtcttcttt ttccaaaaatatattatgacaaacacaggattctgttacagcgtttggtttgccacagtt gcaatgccctgtattatccagttgagggcagctatttactgatttaaaaaaataataatt gaattagaatgttacccccttgtgcttaccaaaacttggtatccactaaaaaactccaca tttgaggtacaaacagccttaattgtgttcacttatgttttgtttctgttgtcattgtaa attatttcatgaaaatgaaaagcctagctttacttcatagtaacttttggtctcttagaa actagaaactgttgattttagttttaattttcccaatccaagttcttttcaattctaaat cttttttccccccgttaggatcagactcattgagatcagggattttcttatctttaaaat gtctgcatgtaaatacatgtgactttagagttctttgagggcttttaaatgtaaaagtga aaatttataatattgaatacattatctttattaaccagtggaataaccactgatggctaa ctaggtctttgattcatttttctaaatgccaaatgaacatgttaaactcgtagaatatat taccctcttctaatttttctgcattggagtatcacattgttaaaattgtatcttctatgt agttcaggaaaagaaattcaggggggttaattatccaattgatttcaaaggtatcactga gacagaatttttttgaaaataatctcagactgcagaaaactgttgtaatattaacaggta gtgttagtaactgagataaggatattccatgggacctactttagcaaaagggattgtcta atgagagaaggacaaaatatgacatatgtttcacaaacaaaacaattagtcttatgttgg aaaataaagtgaacatctgaggagatgctaagactagcatgaacctagtattcattctgc aggatagtaagttgtctcatagggctgtagacatggtatcttggatatcattctcacttt ttttctactaccagtttataggtttccaacactctcatattatctcttctctctcctaac cttagatctctcgaggtttttttggtgatgattattccattttcaatcatttactttttt gtttaaaaacatttaaaatttatggttaaatatccataacatgaaatttaatattttaac catgtttaagtatacaattcagtggcactaaatatatttacattgttatacaaccattac cactatccatcttcagaatattttcattttctcctactgaaactctgtgccaactaaaca ctaactctcatttccccctctccctgtctcagacaaccatgattctgttttctatctatg caaatttaagtattccaagtaccttatataagtggatcatacactatccttttttgactg acttattttacttagtatagtatcttcaaggttcatgcatgttgtagcatatgtcagaat ttccttctttttcagagctgaataatgttcccttgtttgtatatgtcacattttgtttat tcattcattcattgagacttgagttgcttctaccttttggctattgttaataaggctgct ataagcatgtgtatacaggtatctgttcaagtctctgcttttaattatttaaggtatata ctcagaagtggaaatgctggatcatgtgctgattttatgtttaaattgttgaggaactgc catactattttctacagcagctacattcattcatttcacattgccgccaaggatgcacaa gggttctgatttccccacatcctagcccatgcttgttgtttactgttttgttttgtttta aaaccatcctaatggatgtgaagtggtagcgcattgtggctttgatttgcatttccctaa ttagtgatgttgagcatcttttcatgtgcctattgaccatttacatatctttggagaaat ctgtttttgtgtagcattgctgccagtgattttgcatttttaactacattttaaattgtg gtataagagaaaggtctactaataggaatgggactatttcttgtgcatagattgccttcc cagcagcccactttaatatttagcaatgctaatctttccaaagtcaaaaagaaatcaccc atttctaactttctcctttcaaatcctttcttgtctccgtcaaaattcctgctataaaac tccataaaagcttggaagttaaaagttggaaggagagataagtggagctgactggctgtt ggaagaagaaaatagaatggaaagagagaatagttgattgagcactgtgaatggatagtt cacaatcattatctactatctgttaggaatatggattatagtataaagattaattaacac ttgcacaaatggttaatttagggaaatatttgagataatgctggaaagtttagtttgggg ccactttgaagagagtcttcatctccagactttatcttaggccgtggagaaccattgaag gttcttgaggagtaaacggcacactgcattccctctctgtgttttaggaagatttatctg accatactgtgaaggagaaattaggcactagggatttgcaatggactggctagttactct cccattttactataattacttcccatcatagattactttataaataaatacactttttac atgtctttaatcagttgggtgactgttatgctttcaagtataatcaataagtttttcaga attaaagattgacaattgaaaactctggtacaaagattaatctgaagtgtgtattaatag ctattagagaaccagagtatacagtatttaatgtctagtgagtatgagataaagaatggt gtgtatggaaaagggaaagagagcacacacttgaaaatagtgctcattataatatatcta aggtagaacaaacatacatatgtgtgtgtgttttttttaaaccatacgtaaacacagttc aaaaaattcaaaacaaaatttgtaacagaattcaaaacattcctaaaatattaatgctga catttggataaggaagacttaatagatactcaagatacaatgtttgttttttttatttta tttttgtaagtaaaaatttactttcactttctaggaaacactcttagattagttagttgg agaaatattaaaagggaccttacagcacagccctgctttggttgtgttcacattactgac tttgtatgagaggttcaccagctgtttgactggcaggagcaggagctttcacttcagtag tttataattcctggctattctaggatagtttgcacttcaccaagcctcagacaggtcagg acatttggtaggggaaggttgaaagacaaaagcagcaggccttgggttctcagcctttta aaaactattattaaatatatatttttaaaatttagtggttagagcttttagtaatgtgcc tgtattacatgtagagagtattcgtcaaccaagaggagttttaaaatgtcaaaaccggga aaacctactctaaaccatggcttggttcctgttgatcttaaaagtgcaaaagagcctcta ccacatcaaactgtgatgaagatatttagcattagcatcattgcccaaggcctccctttt tgtcgaagacgggtaagtctcacacatttgaaaaatatgaatgtttatttaagtgaagta ccttggcctcttttagtgaaaagatgcctcaaatttcagtttttatttaaaattaaaact atcaatgcattgtgtagccagtaaacacttatataagtgatagttttataatatgatata gagcctagaagatagggttacccagttcacctttttctctcatttatttttcatgaaatt agaatcctgcagatttatctaaaattagtaaatgtaaaaaggtatgttttgaaatacgtt gacaaatattagcactgtaaatttgtagtttttgcatgaaaggcaaaaagttgatggtat tctccttatacatgggaaatacagcatttcattcgttaaaaagtgttccaaatccttagg cttctttggcctcttaagctgtattatttttttaaattgctattccttacattcaaactt aataggaacactactttttgctgttatataaaataaaaatagcatgctattgtgtggcag ttacgatatggtttatatatagacaatcctgaactaacaaacattttgtagaacttgtat gatctattggatgtttctcaggattacgtactcctctccctggtcaacaagttttaaccc tgttaacatgagcaattttttgtatttcctgagttaatttaaattgccagtttcaaatta tttattagagtgatatatttttagatctaacataggtttatgagcctgaaacaaacaaac aaaccactgcttgggattaaatgagtgtatttgtgtatatcaacactgttagggttctat ctttttgtgcgtttcttaaagttttattttgtttttaattgacacataattgtattgtac tttttcttttagctaaaggggaaatattctattaaatagaataatccataacactggaag aatatttttcctccttaataaagagaatcttctatttgctggaacttaaccttcataaaa acatattttctagttagcttagttctttttgagtagtttccacttctacgccaaacaagt ggcagccatgttggaaaacttttacatgaggttaaaatgtaatacagtttaaacatcgaa aacagaatacaaatatccccagcaccctcttgttacttttgtactttactgttttaagaa agtgtttgttttattttgtctgctttcaccttgtcattttagtagcttcttctgaatgag gtcactgctctgatttgagtattttatagaggcctctaatagctttaaagtcactaataa actaatttattatcttatacccctgtaaaagaaaataccaatacaaataaaattaaaaat tgagaaaaagaagtgtagggctttttaaaattaaagggttaagatgttaataatcaagtt aatttttttattgaatatttttaataaacaagtacatatgggagattgcctcattatgtt acagtatatatttcctgtctttgtcttctaacatggacattaagtttaaaagacatagga gaagattctgtaagataaattaatagagaactgtaaggctttcctgagggctttctgggg tcactatttaatttgacttttctaggttactgtgttatgttaaattatagccttgcagtt gtaatagcagaataaaactaaatattaaaaatgaaattcatattaaaatttttagaagat atttaaaaaacaaaatgaacacattcaattttagcctgccggccaataatttactattgc ataactcattgactctgactactagaaatattagagggattgtgaaattatatcctttct ttccttttgcaaatggttagttgatttacattttctgtgttatttattaactttcccatt tttctttattagaaattttaaagatatttttaatatgttataattaataatattttgtac ctgacgtattgtatggcttttacatcgggtatttttactttctttcaagcagattgctat acaccttgacaacaaagcacacaaagcaaacattcttctgtattttacagaccacttttt tccttctaagtcatgagctgaatgtaatgtatagttattgcttatgagactatatgcagt cttgatctattaaaatttgaaagcatggtattttccttccagtcatcatagatactgagt caagttaatgtttgcacagttaatattttggacagaataaaaaaagtttgaaaatagtgg aaaaaattttaaggtagcagtaataactataacttcaatttatatctcttcaaatatttt caggaaattaatttaccttactattaagtgtaatattagtactggtagttaatgtattat ataaattattctagctgctcatctgagataatttataagcattggatttaatactaacaa cagtaaataaatagcaacctagctattaatcatttaccttcaaatgaaaatgtttttagg ccaggcatggtgggtcacgcctgtaatcccaacactttgggggccaaggtaggaggattt cttgaacccaggagttagaggttgcagtgagctgtgatggcaccactgcactccagcttg ggtgacagagtgagaccttgcctctgaaaaaaaactaaaattagaagagagaaagaaaaa aatgtttttagtcttgcagtcctcataatttgtttgtgttttctcagattttcctggtca taaatatcagctggaatacttactaaaaataagttctgcaggtatttcttagtatcagaa aaatttgggaatacaatttttaactaaaaaaagccttggttgtcttttaagggaagataa attttacaggaaaaaattatttaacctataaaataattaggtaaagatatttaaaataac cagttattttgtaggttatagaaaaataactattttttatttgaaattggaatgcctaat tatggtaatgttatatttcttaaatcctaagatgcattattttttcacattataagcata tatgaaataaggatacatcttgatggcactagatagcttacctagcaacatttttttctt tctagtaataagagaaattatattaagatgcagcttacaactaataattgcatggttttt tgttttgctttttttgggacagtgtctcactctgtctcccaggctggagtgtagtggcgc catcttggcttactgcagccttcatctcctgggtgcaagagatcctcccacctcagcctc ccaagtagctgggactacaggtgcatgccaccgcatctggctaatttttgtatttttgta gagatggggtttcaccatgttgcccaggctggtcttgaactcctggactcaaatgtttta tctgctcgcctcagcctccaaaagtgctgggactacaggcatgaaccaccatgcccggca taataagtgtatgttgaaagaaaatggacttttaatgcataaatttcatctcttgactca ttttgtttttgtttggagcacttttaagcatttagaatattctcttaaaattattcttca gtgttactattttagtagaaatttccaaatgacatgcaattgttggtttatttgggactt aaatatatctggtttgactgctggtttatagacaaatattgtatttagagtgcctcctat attcttgagtttgtgtagcataggcatcagcaacacaggagagaatggagtagagctttg gtggaaaggatggagcacagataagggacaaggatttatgcatctgagacccaggaactc aggaaatagtgaaatatacaactgacgtttccagtagttgaaaataatttcatagtgatt atattctatatcaaattctattccaaatactgtacttaaaattatttttctttaaaataa aaatagaaataaaagtagaatatttgtgtgtcatttgaaaaggtgttactgtcacatctt atgtatcgtcatcttaaatcaggagtccacatgctttttctgcaaagggccagtaaatat tttaggctttgtggaccgtgtagttggcctctgttgcactcagctctctctgcctttcta tcatgaaagcagccacagacagtacttaaacaaataggcatgactgtgttttaataaaat gttatttataaaaacagtgaaaaacaggtttttactgttacaacagtactgtagtaaatt ttcttttgcatatagctttttgcttgtgtaagtttcattttatggtgagtttttagaatg gggttctggagctttaagccattactttcctttataagactcattttctctagttccttt gtgtttgtgggaggaagaattttatttttgcgtatctaatcactctgatttttttttttt ttttttgagacagactcttcctctgtcacctaggctggtgcgatcttagcttactgcaac cttctccttccaggttcaagcgattatcctgccgcagcctcctgagtagctgggactata ggtgcccaccaccatgcccagctaacttttttttttttttttgatatttttagtagagac agggctccaccatgttggccaggctggtctcgaactcctgaccgcagttgatccacctgc ctcggtctcccagagggctgggattacaggcagagccaccgtgcctggcctaatcactag aattttaacatactgctttttattatttatcttctaaatgtataataatggtattgatga tttctatcattttaaaagagccaactatgtattctaggtactcctcagctactttttata ttttaatccatttaatccttaaatagtttaaatgttaatgaaacaatgtaagttttatct ccattcagtagatgagaaaactgaggctcagacaagtgaataactctcccagttttccag gtagttcggaacgctggtatttaatctcaggactctctaatgctgcaggtactggctgtt cttttactatgctttgtaacatatacattttttttcttgtgaacttaacacgtttcaact attagtattttgtagggttagtctgggggcctattgctgttgacattgtttctatgagac aatacaaagtttcaaacagttgacttacaaataatttttgtagcaccgtccctttataaa tttaaaattttttgtgtaatcccttttaagttttttttttcctttttagttatagagtta tctcacagattatgtaacaattttagtcatgaaaaaactggaaatattcagaccgtatgc atgtttgtcctgtgtctttttgtaaactgagggatgatacttcagggtctggggtcaggc aactgagaacagggtgcctaacaggtgactatcctcaaaagacaatgcagtaccattctc atcagttaatcaattagtacttttgacaattatgaccaaaaaaaaacacagcacctctca taggacctcttagcaatactgtgttaggttcaggtttttcttcattgttcagttattcat gataatagacaagcctatattcaaaattttaatcttctcattctcataacaaattagtcc ataacctaatttgttgtttttgtatgtataaacttcagttagttcaaggggtgtataatt ttaatctattatactcctgtagtagactgttgggtttgttagaacttttcagcaaggtct gctaggcttattaaacagtcgtattgcttttgtttcagaaaataacatctctaattattt attctacaataaaattttcattaatatgaagattttatacatacccaaaacaccgaccat cataagctctgttggtaaagcaaatttgggaattattaccctcccaaatttagtaattta atatcctccaaagtctgagttcaatggctatataatgaatttttatttccctccaatatt taatatcttttacttctctttataaaactattagacatgttaaatatgtaatgtagaaaa ggcacttgtctttacagtgtcctaaattattagtaatctcacgggtcagaaataaccaca ataaggattttaagcatataattttttaaacaaaactgccactttaattcatttttaata tactgctagtattttcccatggctgtaggtattcaaaagtatataatctgccatataaat atatcgtaatttctcactattgttgatttagattgtgacctgctttgaacatcgttatgc ataaatttaaccacatcactgtacacagatttctacaagtagttttattgggtcaaggag tgtgaacttctttagattcttgataaatattattaaattttttcagaaaaaattgtatca gttcaaaaacagcatggggtttctaatgttacaagacattatattttaaagcacatttat tcttatggcaaagctttgtggactttacttagctgatactgtatcattgtacttccatgt ttattattctaggttttctgtgaactgaattttcattttgagaaaataacgatatatgtt tttacttaaggcctcataaggaaaatatatcaattgattaaaaaattatttgtcgctcat agtgattttattttttattcttataggagataatatttgccatagacttgtcacctatat tattttcaactgtaattttcttctattcttttcatcgttttaaaactgtgatctgtgttt ttatctaaatagatgacccacatgtatgatttgataatttattattgattcaacatattt tttatatggagagtcttatggttgtctcattttttgtcacattagattatacttttacat ttctgaattttagcatggtatctttacagtataagtgacaacctctcccaaagtatatag ctattaataatctctactgtaagagtacttctcatgctagcaggagcagctgccagaaat acttgcctgtttcctatagcactgttacttagcattacatttctgtcatagctttgtcat ttagccccaatcctggtttgccttaaaaaaaaaaattctagtctagctatgattaattct catttaaagtagttgtagcaagtctcctcttctaaaatgtcttgatgactaaaagggatt tcacgaaactcaacatcactttcatagaactcttagccaagtaaaaatatattgatacct ccttaacatacggaaatataaaacgttttaaaagccagcatcatccccccccctcctgta actagtctatttggtatccactggttacatttgtgcctgtttttctacatcctttccatg acctcatggtccagcttacctctctcttgaaacctcccttctggcactggtttcatccac tgttgtcctcaattattgctgttatctttggtccttaggcatactatatgggctgctcaa tgatgttatatgacaactttgtatagatatctccttggttaggttataaactcttacaat tcagaggttatttttaatacctttttatgcttccagagatatgcaggagaaagttagtat aaatatttctatggctgattcattgaagtgtctgttaaacttggaaaaatccttaaggaa tttttgtgctgtaagaatgtggcttgtttttgtttactaaatgtttttaagtgactgtgc agctgtgattttgaatatttcattttctaaatttaacgaaactcagatacagtttgacac ttaattttaaaagtaattactatgtactaattttgaaaatgaaaaagaaacacacatggt atagtcatttaggtggtacttttgggagtctagagtagctcctaaatatcagaaaatgct gaaatagagaaaaaaaatacagtactttgaagagaagaaagaatagggtggtggtatgag caaatttagagagggaatttaatagggtacctgttttgtggtggggtttttttggtttgt tttgtttttgtttgtttgtttttgagtgaggaaactaaaatgtggctttctaagcttctc ttacttgcatatatcttccattggtacttggaaaatataaacatttccagatcctttggt gttttttatggtcactacttactttatcaataaaggcattaattactgtcagttatcaca tatcagaccattctattttagatcctttggtctttttctctttaagaatgtttcacacat catgcatcaacatgagtaagcaatgtaattgtcggttttctttgcattctctgtgctgtg caagccaccggctataaataagatgatgatcccccatgcccatgcacaaatgaaaagtca cagtgataaaactacaaacaaaccattatagtacaaaataataactgcttaaatggacct acgtacaaagtactgtcagagtatgaggaggcactcagagctcaatatgagtggttcagg aagggcttcacagtaaccctgaattaaaacatgaagatgtttgctaagtaggtgatcaga ggagtgatagagggaacttgtatcattgcttgggatcgtgtcttgtcttatgttttttca tattaagtatactgctgcaaattacatcttgccgtcaatttattaatgttgaaaaaatac gtattgagcacctagagtagagaactttcctagaaactgtagatataacagtgaacttga tagacaaaggccatgccctcatggagcttagattgtagtttggggagacagaaaataaat actactgttaatattttgatgacagtaaaatagggtaatagaataaggtatgatttggga tgtagaagaggcactactctagttactttctatacataaactcatttaatccttaaaatc accttgtgaagtacgttgtattgtattttacctgttttacacgtgaggaatctcaggcat aggaaggttcttgccccttagctagtaagtaatgtagctaggacactaactcaagcagtc ttattccagcatcacagccactgcaggaaacggatctggagtaggcatttgttgaacaga cccttagtgtccaaatgttttaagggttaacttttgccatataatcttggaattttaaat gtgtaattctcacctttcccaacaatttatgatggtctcaatatctaattactgataata ctgccttctgtatgtttttgtcatcagatgatgttttagtattgggttgtgccctagttg tcagataggagtacaaagcgacttagcacatgatttataaatctgtaactacactatgga aacaacttgtatgattttgtcttttatgtaacaaatttttaattaggtaacaatttgtat aattttgacatcaaagggcaaggctatttaaatttgtaaatataaactaagttaggtttt acagaaactaacttaatgttgaggcttgtaatatagaagacagatgaaaaatatgctaaa aataaacttggaactaagctcctggcaaggtaaacaagtgtgaaataagtccacagtccc ttgtctgcaaagcagaaatccatcatgctctgaaaactgaaaatttaaaaataaaggtag caaaacctaacccaaactaacataaaacagtcatctttattatttatctgaatattcata tattttgctggagaaacactaatgtgtgatatactggaagtgctctgtaataaatagtat atgcactctgttacctttctacaattcaaaatattctgaatttaggaacacagctgtctc caagagcatggaactaaatccagagaccttagagttttacaactgtcacccactcataaa aaactgaacacactttgaaatgtcagagtagtctaagctttatcttaagtctctgagtac atgcatattaaatactttaaaacatacatatatagatgagtacctaaagtttattaacct aattataggctattataaccatataatacaatgatagtttggagcttttatattactttt cttcagtgattaaaatacagctcatgctaatagacttattctttaaatgagtgtagagaa cctcaaccacattttttttaattgtcccagtatttccatgggaaattaacaccacaaaat taattttaatctgctgatatatagtatgcatactctctgtgtgtgttgctactgttacaa agatgaataagacagcacacctttcccttaaggaatccacaaatgccttcatgtctctga gagacttgtctcatcatccacatttcatggtcacatatcaaaattagatgaacatggctt aattaatttattcaacaaataagcatctgctaatagccaggcccagttctaggcacttgg gatacattagtgaacaaaacaggaaaaaaagtcctttcaacattttagcggtcctgagaa gtcctccaaggcaagaggcagtttgtgccaacatacagtgtgtcatcagactcctttatt aaattatacacttttcatttctaaatcatttggtgataatttgtattatagttttttcat atgtatacataactacatacatgtacatacaatttacctgatttgatagaattaagttag tggccttattgaaatgtataatctgccggttttcaaatactgttagatagtatctattac aagcaaaatattattttgatgctctgaaatgataaataggattccatttaactccctttt taaatatttttgtgaaacaactttcttttggtgtttttaataattctatcaggcacagct tagtgatttctttctgaattattcctggatcagaattagacttcttgtctttttccatgc cactatggaaggttgcagaaactagcatgtatctcacatataaattagttatataatggt agataaaatttatggtagatatatttgtgttatattttggtataaataacagtagatata atttaagatttttaaagatgatattaattttactatagaaataatagtggaaaggcccag catttttggtaaagttgatgacattctaaatgttagcagtttcaattcagttatttcctt tcacaagtacctgctgatgcagcacgtctctttatttgtatatatagtgttttactccta aaatatgaagggtaaaattattaaagtagggtttttttaagtcaatatcttagattaata aagttttcttttgtgactttgatagtgtcttgtgtttatatatttataaacttcattgca atttaggaatgggggaagcagtgagtctttctcaaatattaatactgtttttgtaattct gtttttctatactgaatatttttcagagctatcgaaccataaaatagcaggcttttcttc ttaggagagccatcatttctcaaacgtacatcttactacattggcattatttgcatattt gtttttctttgagagagagaatagttgaaagtggccattgaaagtagcacctccaagtgt agataatgctaaaaacattgcagtgatccactatacatgtaatattttgatataattaac ttgaaacattaattttatagtggaattccatgtttgcattcattaggaatttactcacaa ctaaatcaagacttttaatttgaactttcttcctcaagtaaaggtagcactccccacccc atacataaaagagacagaaaaggttaaatatttatcccaaaagcacatggctaattagcc atttgtttatgattattttgtggtatcctcatataatgatgggaaaatctgttttgagga tttgagaaaatttatggtaatgttgaatgggattttcttaaatttcttctcctttgctag tcttgaacatgaaaattgaaacttatgagctatttaattacatttttatgtgtgggtgat ttcatatagttcacttgggaacaatttcagcttaaacagcttttcaataatccatagaaa tctctgcgttatcactttagattttaaaatgtcactgttgctactaatatgaacatgtag gggcagtatagtactgtttttgtaaatcttgtctgaatttgtgagttttaaaaatctgtg ttactgttagaagtttgttcttctctaagataacataactatatcttgcttttttaaagg atggttggtactgcttcattaagttggaactattctttcagttcatgttcttacatgtgt tatacaaaatctcttatattatgaaagctcttggccctgatttatatttgaagtcaagga atatgtttagttaaaaggaaaaatgcatttttattgtctgacaataaaaatgagaataaa tggattcataaaaaccggaaaataccaagatcagtgcagttttgcccttactctgtgtca tcctttcaaaatgaatgtttatgataatttttacagtcttaatttttcaatatgacaagt gaacaaaactgagattaccgaacagtcttatttttaagattctttaattggtacatcttt ttttcgtgtcaaaatggttaaggttattacaaactttattacaaaatatagtaacttttc ccccgacttaatatttaaaagaagacaactgagcagttatctcttccatctatgtattca ctttattcaacacctcctatgagccacggactgctttaggcatttagagtacaacagtga acaaaacatcccagccatcaccctcgtggagttcatatcagaaaagcagagtgcctaaga gcgcggattatagggtcagttggacctgggtttaagttctttgctgtaccacttactaag ccttatctgtgaagagctaatagtagtaccttttacagagggttgttgtaaggataaaat gaaataacccatggaaagcatggttatgtacaagagttctgtaactattatgaagccctc ataagacatttttagatgtgttggtaaaatttataattaattctcctgatgtaatagtac ttatgatagagattttgagtatttttttcatttttagtttcttcaagtggtaaaagaaga tgcacagcataaattttgaataatttttcttaaacagtttcctgagtgacttttaatctt ctctgagtggaaagataccatgcataaaaagttaatgtgatttggttctacttacattat gtagattcattcagtgcgactatcatatatgtaaaaattaaccccattatgtctccctac atgaagaaaagcatatgtgacatcttggtagtttgtttggagattctcagatgttcagaa taaatgtgttggtttcaaatgtcattaaattggaatttttaaaatcgctatataagaatt aaaaatacatgttattgatttacatattatgttagctgctaaaatgtctagtatatagtt tccattataattttaagttctgacaccattttcctactgaagcccccaaaatttggtaat taagaaatgtaacatactttttaacaaatttgaaaactgtcttgtcaaaatggtacctga tatatcattactacataagaattgagacttaaaacatttaaagaactgattcacaaattt taacaaaagtacttagaaaattagacctttctttagtaccctgtgcttctcttggttctt gttatttctgtgttatacagaaattccatatttaacacacacacatgcatgcatccgcac acacgggcatcccacacacatagaagtaaaattctaaccaccccctacctctatttaata gcttatcagctatgttcatgctacaactgcttcattgaagggaagtcctattctgctgtt ttcatattagctttgttataatgcagtatttttcagctgctcatgctcattaccatacag ctggtatgtagagaagagaaggaaacaggcagaagttgtactgtctacaagaataagaag ctctcttgcagatggggtgctggcagatggggcatgtgtttgaacaggttttactaataa cagagtgcgttaaaaatcattctgagaaagtcctaaggtcagtgccatggattcagagct tgagaggttgttcagctttttcgtagacctttttagaatattaatcgactcttacaaaaa aaaagtaataaatggcaaaccatacctcctccaaaaaaaacttaccagtttttttcttct ttatattagtatgttttttctttagtataattttaaaagcattaatactgtcacctagta tcatcaacttgtgttgtcgtagtcttaaatatatttacttaaaatgtctacatctatata caaaaatgtagatgctttgtgaaaatggtaataacattcttcagtttctgtatattagaa tatttattagaaagtgaaaactaaagatttagatatcagtgtgtatagtgttgactacgt tgaagattaagtcgaggaatgttttatctgcatacaaagttagaagtgaatggtatgccc agaaatgtatctattgttaaaaatatttcttgcatattttgcatattcacaaaaccaaag tgaatgaagtataattaaaataaccatcatttttattagagtattttagtgggatcttag tctttttggcccttttttattaactagtatttcttgatatatcctctctcaatgggagat gctgatgaaaagcaactgaagcaaaataaagctaggcaattttgtgtaacatatgtagta ttcaatttaattttaaaggaaatagaacttgttcttttcatagagattaaaatatgtgaa tatatataatatatatgaaatgtatacaagtatagctagtttttttttatactttaagtt ctagggtacatgtgcacaacgtgcaggtttgttacatatgtatacatgtgccatgttggt ttgctgtacccattaacttgtcatttacattagatatttctcctaatgctatccttgccc catccctcaaccccacgacaggccccagtgtgtgatgttcccagccctgtgtccaaatgt tctcattgttcaattcccacctatgagtgagaacatgcggtgtttggttttctgtccttg tgatagtttgctcagagtgatggtttccatcttcatccatgtccctacaaaggacatgaa ctcatccttttttatggctgcatagtattccatgatgtatatttgccacattttcttaat ccagtctatcattgatggacatttgggttggttgcaagtctttgctattgtgaatagtgc cacaataaacatacgtgtgcatgtgtctttattgtagcatgatttataatcctttgggta tatacccagtaatgggattgctgggtcaaatagtatctagatccttgaagaatcgccaca ctgtcttccacaatggttgaactagtttatagtcccaccaacagtgtaaaagagttccta tttcttctccacatcgtctccagcacctgttgtttcctgactttttaatgatcgccattc taactggtgtgagatggtatctcattgtggttttgatttacatttagtttttaaagctag tataagttacaacacttttggcaactcagaaactcatgcttcagatttcttgtcagtaaa atggaggtacaaaatactgtctgtgaagttaataactatgaataaataaaatgatttaaa tgagacctgtgagaagtggtgtactttaacaagcattgctaaaactgtgtattcctttta atagtgaaagtttagattctgttgggcattttaaaggtacagaagtcctaaactgtgaag ttgcatttttattgctcatcaaacttattttcttccaatttaatttgtatttggactgta tgactgataaatatgtagaaatgcttttatgacttttccctgagggggaaatatattaag tattataaacaatagacagtataattataaaatgataaacctgtaaaatgtatttttcac ctacatcatcatttcttatttagaaaattatgagtttttaaaattgtgttcaaaattaat ggttttctgttgttatttctgtagctctaccattgttgattagggtatgtcttgtgtggc taattatctcatataaatattttttattggtaccctactatatgcaggaactatgtgagg agctgagatacaacaagaatcaaaacagaataaaaatactgccctcatggaacttacagt ctattggagaagacagacaaatatttaatgtgctcacactgtaacaaggtgttggcttca agcactgggaaatcagagatagcctgtatacagataattcacatattttcatctccaacc cagacctttcttctgggccagaaccagttggagcatgagatttggatacccagttatctc caagatatctcaagatcctcctcacccctggcccccagaaaacaaaaaacaaccaactaa cccagtccttgttatccaattgcacaagtgagaaaacaggtgttatctttgataccttcc tcttttttacttgccatgcatgacccatctgttactatcctatcagtttgatgtcctaaa tgtctcttaactgcccactcctatgcatctctgtttctacctagctaatcgaagccatca ttatctcttgcctggtctagtggaataacttcctcacacagtctctccacatctactctc aatcactgccattctattttcagcattacactttgaatgatttttttcaagaaataacat ctgattatatctttttcttaactactatgcagcattagcttttcattactcttatggtaa aacccaaaatcctttgaggtcttgggtaatgtatcccttgtctacttcttcagcctcatc ttacacctgccatcctacctctgttcagtgtcctctagctataggtggttatccctccct gctccgaacctagctcagtcattcattcagcgagaagatgccttctgtgaacccacagac taggtggaaacctcaccattcttaacactcttcgaacactgcagcatttaccacagttgc agttttgctttgttttgtctcattatcttgcccactatgctgtgaactccagccatgaaa cagacacaagacttagtttagcttatgttaggcaactaagtaactattatattgaatgaa tgggagtatctgtggcagactgaaataaccatgtaaatctgcatgtgttgtaattatgta tattaggtagaatataattatggagatacttgagacaaagtgtagggtagaatcacagag atacatatgtttcaatacagatgttcactgttaggaatgataaccatacatggatcaaat ggacaattaatgaagatggattggctctttaaaaaaggccttatgtaatgaagttaagaa acagggcccaaatgatagccacatattgaaaagcatgtgctctatgtattcagatggaaa atcctgtgtatttgttactacttggttagtaagtcaagtatttttaggctgtgtactaaa aaaattttcaatgaagaagtcatagtcccttattaagtgtatttgggtttgagtaatcaa caaagaacatttgaagcagtttctttcataaatattatgattcataacttttgccagttt tccatagggttcaaatcattttggtaaggaggaaaaaacctagtagcttcgttgaaagga cactgcaatggagatcctttgatgtttgtgttttcagctctcttttatttcatattttag ggaaataaagcaggacatgaaggaaggagagtaagacatagaagtcctgagagggtttgg gaaaaggaagcaaaacccatttgttgttgtgtttcttccagtatgcccactgatgactgc ctggcctcaatataatgtcacagattataagagcagttcttaaccaagcggagccactgg cagtagtgtattaacttgttgtcaagctgctcctggtgaattcccagaaataaagtgtat cacagtacacaattagctgcatagtccgtctcttctttctgcaagtctgcagtggctcta gtgtggatttcatattcacatcacacatttgcatactgaaataactaagaaaacatctta gagatatttaaactgccaatatttcttgctttgtatatagagaaccctatttttattttt ttgtaaactgaagaccaggacttccttaggttcaaatcccatctaccttaagtgaccttg gggtcgcttaaaactctctgtgcctctttcctcatctgtaaaatagagataataatagta ctttctgcagaaagttgttaaaccgtttcattgaattaatataagaaaaacacctaggaa gagtgcctgacatatagtgagtttccttccctgtatacattgtattcttaagaaaacatc tttttgaaaaggagttgcttattcaaactggaataaagtttgcagcatctttgatagtgg gcactgaaagagcattcaggtcagtttaaaatctagtgtttatttttcttgtatacattt ggtttaaaatattgccatgtgcatttcttgaaaatattatctcttcctctttctagttgt atatattgtcatacaaattaagcagtttcacttattagtcaaatggatttgtcatttttt attgacttgcctgtccttccctcctgagttgggagtgcttccaggatagatattacatct tgctcaattttgcatcttttttgcttagtgcttggcttttagtaaccctggacaaatatt tctaaaatgtggtagatctagatctaaaaatataacatttgaatcactgcttaatttttt ctccctcttgttttattgtttagaaaatggagaatcattatatgactcaggattcatttg gatgagactgataatattataatggaatttggagttagaggctcacatgaattgctctca gtcactagctttttctgtgctacctataaatgataaacaaatattgtgaatctcacaaag ttaaagtctttgaaataaatggattggtagggggtaaattacatcttagtctaacaaaat ctgtttattgcaatggtttaataaaagtcaggtgactaaaaatatcttggaaaataccac ttaattttaatctttgctgtttatgttgtgatcatgatgattaacatatcttcatttatc ttctcttgaaaactattccaagttcttgttgtttttttttttttttttttttgagagaga gagtttcactctgttgcccaggctgcagtacagtggtgcaatcttggctcactgcatcct ctgcctcccgggtttgagcaattctctgcctcagcctcccaagtagctgagattacaggt gcccaccaccatgcctggctaatttgttttatattttcagtggagacggggtttcaccat cttggccaggtgatggcctggtgaacttgatcttgaactcgtgaccttatgatccacctg cctcagcctcccaaagtgctgggattacaggcatgagccactgcgcccggccctaagttc ttttttaatatgctaagttgtctgttgttggaatgactttaaaggaaaggtttaaaatga agaaaggaatatgtgtgtgtcttctttatcatctcatgaactaaaaaatactggaatttt aagattacactaaagttcagcatccttttgctagctttgaattgatattcttttgggtag atttagactattcctcataggattttttcatattgtataattatgaacatacattgactc ataaacttagattttaagaaatctaagatttagctttaaaaagtggtctcaatttaccat gtagcagttcgtaacagaccacagctatatgcacttgttcatatcaggagcagttcttaa ttttagttataattcatatttgtttaatagtcatcattccatcattacataccatgcctt catgcaactggtgggaaacttatgatattcattccacataaaggagtttttagaatcatg tattaaatgtgataagctttcttttttcttttcttttttctgttctgttctgttcttttt tttttttttttttttgagatggagtctagctctgtctcccaggctggagtgcagtgttgc aatctcggctcactgcaccctccacctcccgagttcaagcaattctcctgccttagcctc ccaagtagctgggattacaggtgcacaccaccatacccggctaatttttgtatttttagt agagtcggggtttcactcttggccaggctggtctccacctcaagtgatccaccagccttg gtctcccaaaatgctgggattacaggtgtgagccaccatgcctgtcaaaagttagttttc aaacatttggaggtctcaagtgtttataggtaaatattttttaaatcatgtgctaaaata aattttaagaaagtgaatctgtaaataaaatatttctatgagaatgttgagaagatagca aagtgcttctaactatcagtcttacagttcactgcacataaaagacaaatatacattcag ataccttttcaagttaagttgtcttgaaaatatttattgaccttcagagtcttttggaat ctcctaaacaggtagggaatcatgtaagtatttgctagtcatagaattcttgattttttt ttaaagcaaataatgtaatttgttaaagtatatatatgtatataggaagtactttaaaat ttaaattaatgttttaaaaatgaatagaatcctctttttaaaatgtagtgggggcctgat tactatgttcttccctactcacttgacagctatgtcaaacacttgaatacttaaagaaca agcaagctacataaaaacaaagaattgattaaagatcaccatgaggcacactttctagag caagaatccagtgttatttttgtttctcactggcctagcaccaaaacaaaataggtactc aataaatatttttgaattagtgaaatcagtttgaattcacagactttgattcataactag agttttaaaagattggttcaagttatgccttgtcattttgaattcttctggaaaattagt aagatgcttgatgagaagagatgcctatattttaaaaagtgcagtaagtatgaatctgga ctttctgtatctgccagccaaatgttggctggcattttactatattttatatatatatat atatttaatatattttaatatatttttctaggcttttctctatgcatacacagtgcgcac acacataaaatatatgcttgttttttcaaaaggaccgtaccttatattgtgttatcgaat ctcacataccttctagttttacagtcacccttccatagtcacctagctctgtacctgatg aacggtttctgagacttcaaaatgctctatagtgaagtctgttctttagaaaagaggctt tgagtctgggggccagacagtagaggtgtggggagaaagggctggggtagaacaagatga aaattttcttagtgagctatacctttttgaataggacaggttctttcagtcacatgttgg caagaatcggtgaattgtgtactcacctgttgccccgagtaacttttcggattgtttttt cattaactttttctgggaatatacaatttcaatgaatgttttatacaccatttccctgcg ctgtgtattcaggagtacaacttgcttgtatgtatgatttctagtgtgaatgcctgttca aacagaaagtccccttgtttgtcagtgtttgtaactaaaataaaaagtcaacagtcttca cacccacacagtaaagttctcctagtacagaaagacacataatgccctaagatggaatga tgagtcatgacaggaggtattttgaagaaatttaacaatatgttaaataacattttaact ctagtattaaatgtttcagttaggagaaataattgttatcacatgggttatcccctaatg aaccttgtaagaccagtgattagaagtctaatagatgctgttaatcctgcaagttaataa tagtcgtatttccatttacatctcaggtccacaggtggtaatttccttcttccctctcaa cacttatcaaacttctgttttttaaacacagaagcaagaccatgtcatttatgaactccc agactattaaaataacttctccattttctgactcactccacccaatttggcaattgatat atagtccagtattgctttctatttgtttcttgtctttgtgggttttttttgttttttgag actgagttttgctctttttgcccaggctggagtgcaacggcacgatctcagctcactgca acctctgccttccgggttcaagtgattctcttgcctcacctgtcttcgtggtttttgttg ttgttttgttttgttttgttttgtttttggtagtttttgtttgtttgtttttgagacaga gtcttgccttatcacccaggctggagtagagtgctgtgatcatagctaactgcaacctca aacgatcctcctgccccagcctctcaagtagctagggctacaggtgcacactaccatgcc caggtaattttttttcattatttatagaaatggggtcttgctatattgcccaggctggtc ttgaactcgtagcctcaaatgatcctcctgcctcggcctcccaaagctctgggattatgg gtgtgagccaccacacccagcctcatttcttatcttgtcacctggattttaagtatcttg tgggaggaaggtaattgcaatgtttttgtttgcccttggtagcatttcgcagatattggg acataggaggtgacttatttctcaattgattgtttttttaacctaaaattttctcagttt ctgtcacctttcccccatcaccaggcctttgcctcagatttcccagatgtagtagaagca tagttaatatggacctaaaataacgattcaataaactgaataatcaagatttacttgtaa tttacatacatactttttgcaaaatataacacttttttcttctttaacaatgtctcgtgt tttagcttaacttgagctaccacatatctaatggaaatcagtaagaattattgatcttgg gaccagagaccaaggttctaaatatagctagctatttaagcatgggcaagacacttaatt ctgtacctacttttcccacctgttaaatacagctaccatttctagggtatctgttatgtg ctaggcattttctgtttcctcacagtgactctgtagtttaggtggtaaggtaggtgtata tcattactgttttataaaaagagaaaacaaactcgtaaaggtcaagtaaccacctaacat tacactctggtaatggcaaaacttgcgcttaaatccaggtttttctggttccattgcttg tgttcattccacaaacaaagaggaattagatgacatccaaaatcactctatggcttcctg ctgttaatattttttgctgatttagtagctataccttttttttttttttagtctttagct ctcttctcaaaacttttttatcatgactaatcactaatttaggaaattaatcccagaatt ttaaacagttcttgaaatgaatacaagattaatatatcacacctatgggaaattcttctc aagtcaggcaaatatatttgaaaatcacaaacagcagcaataaatatggatttgtgtaaa gaaagcagaagagtgaatagggtttaggccttatattgagggaaatttgatttcattctt tctccatgttaaattctttcctgggtcatatttgtggtttcatttttcttgaagtcaaac ttacattttatactttttttccatgatatctgtcttactttactgaaggtattactataa gaaattcacaataaaattttgagaggaaacttatgaattccaaatgcttattggtagttt attttggattttaagcatgaaagattcattgctttctggataaatcagtaaactccaggg tgacttgctaactttgttgtcaaattattgcactgatgccggctgtctgtgaaaaagtag aggatgttgacttagtattagtctgacagctctgtaacaatataaattttagaggagcga ataaaacccccatattattttattctgtggctggcataaatagaactcttaacttgcact attgggtatgaattatgtatgtgtgtatatttatatatttgtagataaatacatttgtgc atgtgtccatcagatgactaatttggcccaatctgaaggaatgaaaaaacttacactgtt ggaactaatactgcagttgattagtgtgtcacactaatcagaggctgtgtctttgtaagg agagttttaaatagcttaaacttcaagaattaaaacttctggccaggcacggtggcttac ccctgtaatcccagcaccttgggaggctgaggcgggcggattacttgaggccaggagttc gagactagcctggccaacatggtgaaaccccatctctactaaaaatagaaaaattagtgg ggtgtggtggcacacgactgtaatcccagctacttgggaggctgagtcatgagaacctct tgaacccaggaagtggaggttgcaatgagccgagattgcgccactgcactctagcatggg caacagagtatgactatgtctttaaaaaaaaaaaagaaaaaactactacttctgtaatta tattaaaatttatttaaaagcagtcagctataacattttgatttgcagcttgatttacaa aggtttggattatatatattataaacatccccttccccccaaaaaataagtttaagtaaa gtaagattatctattacagtgtttagtgtttgggatttttttctaaggcactagtgtcac agtatttgtttacttagatggtagttttgaactaaagctggctcatcttgttttatttta catgttctcaaaaattttttagaactaatttcagtatgagttctaacagtcagatgtttg tgtatattacgttacactatgctcttgtgttaattgactgtatttccaatggagaaagaa caaagtttcaattgatgtatataatattctgtgcccaacacaaagtaggtgctaaacaaa tgcttactgaatgaataaatatggaaaattgcttattggccaatactgccacctatttgt gttagtgaaaatgtaaatgctgggcatatatactattccagttgatatctgtaatagaac aggtttattagtttgccagttttaataagcaaaccttttaataagcactcacatcccaga aagttaatgacagtgagttagtgaaatttcaaaaagtatttcagtctcaacatgcttggc attcctttcattgatgttgaaattttgaactgtctgtaattttgaactgtctgtaatcta aattctgtgctcagggcaagacagctaactgtggtggtttcctagtcctttctagagtac aaagtgttgtcatgataataataaaatcactgacataggaatataaacacattctcctac tattataaaatggaagtgtatttttaaatgttagcaaggtaaagaaaagaactaatagta ttcatagtattttccactaattataaactttttagttgagttaaactttttagtactgat tgtaatatatgtttaagctttagaaaaataatgctgtggtttctgatatttgcttgtatg gccaattcaaaaatattaattatttgttttgtatcattttctggatgccctagaaataaa tttgagtaagatgtgaatctttagggaggagataggggagaaagatgtataaaaataatg acaatgaactatgataactgcagtaatagaactatatataagacggaaataatgaactct tgagagtcaaggtgaggtaaagtactctattaactacatcctttatgttgacattatatg taaagataagtatttctctgaagatacatgattcgcttaagaccatttgtgtatattttt ctcactgtatatacactattttttctttctgctcacaaccacaacttgcccttctaaaaa cttaagtcgtcccaattatttgttcagagctctggtaggagctcacgggttaatgattct atggcatcattccccagaggcaaacatatcatcatatatttgtgttgtttttttttcctc gttacttctaacttctatgctagcacactagttccacttctacacctagcacagtggagt tgtgatgagatgagcattgggtctgagttagcagatggattttattccaggaccttattg ttggtcattccctttgctaatggaatttcaataatgcttctaggttgtcagtcaacacaa gaattaattttttatagaatgggagaaatgagtgcctagctgaaattccatgaaaattat tgtaatgaaaatatatttaatatagtgaaatagtaattctaaaaatatagatgacaaaca ttttcttagctgtttggttgactaacttagctatttggtttcttagctatttggttgacc ttctttcctgtgaacttactacttaatattttagtgtaaggcataaaatactttaaaaaa acttatttaacatagataatagactgatctaaagtttaaaatgtttcttgtcacagagat aagatagataaatacagacacaaacccataaaaatttttaaatattatattaccaaaaac ttaaatctgaataaagtttcaaactatattttagccgagtggtataaatagaaatgcaag tgattttatttgctttgttcatattaagattatgatgacagtaatataatagatcacagt tttcattgatagtacgtcagagaatgttgaaaatcatttttattctgttaatttgaaatt cagttagaagcttagttgactagttttgcttgacagattcttcacagttcaaattaacat cataagatattagggagaattgtttaaatacttgggaagacaaggtcttcattagtactt ataaacattcgcatttctcacttaaaggataaacaataaaaatcattcccaaatgggaac catggtagatggaggatatgtggtagtagaaagacttctcagtgtagaattttttgtata tcattttgatgtttgaagcatacaaacctggtaataaaacatggatctacaggacagtac ccacatacaaacaatatttgtagggaacaggttttcctgtaactcacagccctaagtagg agccatataaccatgggagcagtcctttgaatatacaaagttaagagatattttctttag gttttgagacagtatcccctacttcattaaagttggtctcttcttaactgagtagattta atttgaaaaggttagttcaactagaaataggacatgatttcagttgaagccaaagtatat actattatagcactagtattacacgtgtcttatggaaaacttgttaccactgacaaattc tggatgaaatttcaggtgttctcatagcccagaacattgaggaaaatcaccttttttccc cctttcctaaatcttgagtcctcagaacttctgacaaaagagtttatgggtccattccta acaattagatttttttgtttttttgcttcccttcgaagtgagattatttagataactaac ttctgctgagtacatatacatattgttcctgagggtaagaattaatttaagagccaggta ggagattttttttaaagataagtagacagtagaatataatttctagaagactcaagatgt acatcttcataaaattttacgttatcttaatttcatttatgttttaaagtaaaagatgtt gcattgacataatgtcttagttataaaggtaaacatttttatttttaaaattatgatact actttatgcagtatttagaaccattttactaaagaaagccatttaccaccatagcagaga gtattaaaatgttagtaagtccaaatggttgttttgttttgtttttctggagaaacttac ttcgaatagtggtaactataggcacacaatttacttgtaataaagaacactaatggggag ggtaggagtaaattaactagaaccttaataatgggcttttctagctcaaggaatcttata attgtccctgaaggtagttgtgaaggaagttgcaagatgaaatatctagatttgtgtagt agtcattccaggaaaaatgttgccttctggcaaactttggcatctttattatgtaaaatg cctttttaatgataagcaatatagattgtcaaaataattttggagatagaagtctagtgc ctcctgtttactccagattaaattagcaaataaaagtaagcctctccagccagcacagtg tttgctcttttggggataaaatagccttttggccaaactttacacacttaggtagtacta gtttgttaatcttacaaagatattcttatgattagtaagaattgatgaagtgtatggcaa tttccctgaaaattactatgttttaaaggattgaaatggaaaagcataggcaatgttttt attaaatcaataccaaaatacgcagtttgaaatataacttagattacataatttctgcaa atgctgtcggctccaaaccagtttactttattgtcagtcaaccccagagtatattaattt ctcttttcgggattgcctgttttgtaccatccaagctacttgaaaaatctgattagtctg tgccaggactacggtaggatgacagtgaaaatagcatgctttgcctcagttcagggggac cgtatgaaaatatatcctattttcaaaatgtttctgaaaagacttatttgtggaaatgtt ccttgctatttaaaactcttcaaaaatgcaggttttacaaaatacaaaaataacctcttg aaagtttgaaaggggagagggggctgtcgtgtgggggcggtctctgctgcccacatcctc cctctgttcagtgcgttgtgttcttacgggttccctggagcggatcaccatataattgat gtgcagtctgcattgctgaatcctggactgcaccattctgtgttcaagggaagatgtaat ctgatccctctgctgctgagggaggaatctgttcagtcaaggctttgacagggcatagtc tcctccaataatcttctccgttctctctcattattccctcgagttcttctcagtcaagct gcatgtatgtatgtgtgtcccgagaagcggtttgatactgagctgcatttgcctttactg tggagttttgttgccggttctgctccctaatcttccttttctgacgtgcctgagcatgtc cacattagaatctgtgacatacctacctgaaaaaggtttatattgtcagagactgccaag cagccggacacacgggggcacagaatcactgaaggggaaaaatacagaaaatatgggttt ctacggcacattaaaaatgattttttacaaagtaagtaagctgttttcttcctatgctat gtgattgtgtgtgtatgcctgcctgagaaggaaaatctgtatcatcagagatggctgcag tatcctctaattttggtgggtggctggatgtctggtattctgttttatatcatgaatgta agcaaataacggaaagactgtgagctggaaaaagctttaaatttaagataaaatgtatat tttaatctgagaatttttcactaaaaatacattgagactttagaaatgtggctctgttaa atgctgacctggaaatactctatccttagttggcttttagtgaaccttttgccttcaatt tttctataccttgtatacagtttttcttttttcattagttgactctttaaaagtatagaa tcatgagactatttgggatttatatttaaagagatcatgtttactttctgtacccctcaa aaattgcagaattaatttttggttctgaagtcagtattttcagtaaacagtacagtattt aaaagacttctatagtcatacagaaggtgctttcctttataaaatatttttaaaataaaa tcctttctcagtggtttccttgatacacattttggggcatgtttattcattgaaggattt ctaagatggaaataaagtttgcttttagtggcaaatggtatttgcaccaatgttttttac aaggtatcctgggaagttctgaaagccagtattacaaacttaattagaataaatatcaaa gtttctcctaattatgaagataagaatttgtggaagttatcaaaaaactagattttaaca tagtctgtactgactgaccttcctgatggattcatttgcatgtatgtttatatatatatg tgtgtatctataaattatatacacggaaacacgtggctacctatatacttcagcttttat atttatatttgctttttaataaaattatttggagaaagaaaataatggtttctgagagga atcactttaaaacaatgtaatgagtatataaacttctaaagggaagaaaccagccagatc aattccatttctttccagtaggtatggcatgaactaaggtataaattgtaggtgagcttg ttgtaccctcacctctagcccctaatgtcgtaagatcatcctagtattttagaaaactct tcgtgatgacaaaaacatggtctttattattcagtgtggctctaaaacattttaagtctc tttagtatttatattgtatcatgctatgttatgatgttcccatgtaaggcaagaggtagg aaaataaggctgctgcaaagcaaggatttttacctacttataaacatgaggatagctctc tatcactttggagtgtttttcctcctataacttgcagtaatacagtgattaattagtgga cacaaatcttaaaaaggggttgttgaaatggaaaaagtcaaatcttaccagattttattt tctagttttaaaatatacatatagtcaaatgagaatatttcaatattgatgttgacaaga aacaattctgaatatatttatagtataacatggcataatgaaacaccttaaaatgtagat tttggatacaccttatagaagaggcataactgattcttttaaaacctttctgttgttgct tttcaaattagaatattttgtcctgagtatctgtcaatagagctatattctgtatatata tcaattgacaactttcccctcagcgttattatactatctccagatttctcttattattga tttactttttaaaactgtccttattgcccatattaattgctttatgccttatcactattc aagattatttatactgctctttaccctcttataatggtatgagtgtttaaaattgagttt gttactttcataattatttctgattttacaattttgaattttatctttattaaaaacatg tttttaaagaaaaatgattcacgaggaaggcacgttaaatatgattattttctgtgtgga ttaacttgagatttttccctatgttggttattaaattggaaaacatgaaagatatattat ctcaaggaatatatatatataatgaatatcagttttcaaatcatcacacactgttcttct ggataaaatattaggggggaaaattcacctttccattccattcagagtaaaacaaagtag aacaaatagaagaaactatgaaacatttattttatatatttaattaacattgtaggcata tgaaaatttaagaatttaaaatattaaccatgctgactcaagatttgatagttagacgat ttttaaaaaacatatcttgtttgttttaccttatagatgaaaagaaagttggaccatggt tctgaggtccgctctttttctttgggaaagaaaccatgcaaagtctcagaatatacaagg taatattaaatataatgattatctcatgattctcagttttttagagttgattctgaaagt gttttagtttattcttgtagaataataatgttactagtacggttgactgtttaacacatt ttgagattaccggaaattgagaactatatcattttaaataagaggaatgccaccttagtt tatgttttgttactaatacattttgaagtaggacacaagcagaaattatcttgtgaacaa aaatttaaaattacaatttttaaggaattctaaaaagtaaaccataaaatatgaatatga gcccctgtgtcttgttactataggaacagctgttctagggtagacatttatgtatggaaa tttgaagatacatttattagcagtgaagtaaaacaattactaactcaatcttatttcatg aaattaatgtgttagaaattttcttttatgggcttggtaggtcttaagtttttaacacat acatttcttttctataccagtagaatagtttccataacaatgaaacataattgtaatctt tttctacttcagtccccattatttttggattttacatcagtataaaacttttcccactct cacagttattaggctaacttaagcacaatgtctattgcccagccaattaaaagtggcttt gaatgtcacttggaatgtcgtattttaaaatttaaatgacagaagtatcttagagctttt ttttttggtctttaaaacagaattttttgtataattgccagtttttaaaataagtgattg aaagttaacagtagaaaccataagaacttcatggttatgacatttcttcctttctctcaa aatatttcaatttagttatttttattctataagatgcactaaaattaacattgctcagat ataatttttttattgaacattgttgcagattgtgcctcttacagagaaatagcttgtttt tatttcagttgtaaaaaatatttctttcaaatgctgctttatgaaatttgaaaatcatca taattggaatagtcttacattcaaaaagtgaagagataacacagaaaagaaaaataaagc tatctcttattcaggagctgcttttcggtgtgatttttcttatgttaactgtcccccttc ctgttactctttgccttttgacagttttactgtgttttggcacttccaccagagggtgtg gagggatagagagacaagtgaaactattcacttcattttgcttttcccttctagtcatga taaagtatttcttggatgagaaatttgcatttaatgttaaactaactgttaatgctattt tttaaatgtatcagtcttctattaatatggctgataccaggacaaaggtattacattgtt cacttgtttttaaggcagcttagaataaatgagtgctgaatttttattacagaacacatt ttagtaaaatttgtctttagttgctcactttttatattgacaaagcaactgaaaaggcag aagttttatttttagcctgttaatatataattttattaaagcaaaattagaccaaacagc atatttatttccatgtttttctggagactctcggttaggaaggtatatatttggtatttt gtgatatttttgacagatttgctaataagataaaactatgatttttccatcaaaaatttg ttataaacatatcctattttattttggaggaaaagaaaatagaacttacctttgaatttg aaagaagaaaagatagatttaattgctgaatgttaaagattgtcttcgatttatactatt agaggatttaaagggagtagataagaagacttttctgtaaaatctggtgactgagataca ttttttaacaaaataatgctggatagaaaaaaacagctggaggagttgtttcattagtaa tttatcagagtttgattgttaactattacgatgcaatttgtaagtgttctttgaatttaa aataattaatcaacatagctcaatgaattgagtgagaatttcttttcaaatataattttg ttaaaccagaccttcctgctgctttttttccaaggtctttaaaatggatacccaatattt accttgagtttagtttaattacatgcagaaattagaaaggaaagtttaatacgttagtaa tatagttatatattctttctgctattaattggtcatttttagggtagttagttttagaga gatcatctagtcgtcctcattttactagtggcagaattgaatttcaagtttgtttgagac agttaaatctcaaaatttaaattattgtataaaaatgtttagatgatcttatttagtttt gcatataagttcttaatacttacctgttttgaataaatgtatttctaatcaagactgagt cctgtcaactattcagaccaaactttataaatagcagtaaaaaattagaaatattataga caattttgaaggcatctgaggcaaacttaaggaataatgtgattcttgaaattcaaaaca tttgaacatagtatttctatgtctgagttttttaaagctttaataaagtatcacaaccct ttttataaagaaaatttaaactttacttatgtcaccaaagaccagtcataggagaagtgg gacttgtatgttctaaggaaatattttaactttatttgtatatattaacaacttgcagtt ttgtcatggagattctgttatttggttgcaattagcatttctgggataataacaggcgtt atataggagggaggaaacttactgtaagaatagcaaataggttttgtgtacctgtgatct ctgggaagaaaggtaattaattgatgtttgaaatatgccagatcatcattctttgtacta gaatctattattaataagtatctatctttgctttcacttttgtttttttccttttatcct ttctctctctcttttttttttttttgtagtaccactgggcttgtaccatgttcagcaaca ccaacaacttttggggacctcagagcagccaatggccaagggcaacaacgacgccgaatt acatctgtccagccacctacaggcctccaggaatggctaaaaatgtttcaggtgacatat ctaaacatagtacttgaaatgcctcacttaattgctgcaaaataagggattattcatccg taaaaaaagaaggaaacactgttacatagtatactgtggatgaattttgaaatcattctg ccaagtgaaagaagccagacacaaaaggccacatgttgattctctttatatgaaatgtcc agaataggcaaatccatagagacagaaagtagattaatggttgcctgacaatgaggggag gagaaggactaactgccagtaggtacagattgtccttttggggtgatggaagtgttctgg aattggatagtagcgatggttgcacagcaaggtgaatatactgggaagcactgaagtata tacttgaaaatggtgaatgttatgtgctgtttatttcaatttaaaaatgctattaaaaac aagggaactcaatttttgaatgtcataattgagaaagtccaatacttatttgtgtaactg tgcactgacattttttttattgctttttaaataataagaccttccttcatcttctccttt gtttgaatttctgggttgtctagttttctaaactgcattgcttgtaagttaggcatttaa gagtaatttaagaaccattctagctagagttaatacagcttcttttcttcagtgccatgc ttgtatatggcttaccatgcttgaatcctgattactatattttaatagtatttatattct ttggatgtgtacagggaagattggttacaaccaaaccctgaatattatgaactttataac ctaagatagtgatattacattgatttatctcttcttccagtaagataatgcatatacatc aagtgtttagcaaaatacatggcaaggattaagtacttactcaattatttgttgcaaata ataaataatataaattatgtaaagtaaaaaatatctgatattagcctccatagtaatgta aaaagaatatactaccctacctctggctgcttgaatggttaggtatttaatcaatgcata aggcaataatcgttggtaaagatgatactttacttgacttttagtgtttcagatttttaa ggtgttcactttgtaaaccctaatattttaaatacctagatgaatatgagataaatgtgt ctatttttttcttctgtaggtagaaaactaacttggagcagtattgcttttaaatggttt tttaaaattgttttctacccttcaggtgatttctgcaaaactaggataacctttttttgg ttggtaactgaaagatttaagccttgctctcaacagggattagtttttccccgcgttgag acaaaagtgtctcaagacatttttcatttttataactgaggggatggggcgcaggacact gctggcatctagtaagtagaggcaatacaaaggacgatctccatgccaaagaattatcca gcccaaaatgtcaatggtactaaagaatctgggttagaggaatgaaagttagattttatt gttattaaaatgttataagccactgtaattacaaagaattttttttaagttttcattttc atagtcatttgttgattaaacttaccttttccccagggattattcctacccttcctcctg agttattttgcctctggttcatgatggtttgtaaaatgttaattaatttttattttttcc tatgcacaccctcagaattctaaatttttaattttgtcccatccttttaaaaggaaattt atccgtcaataagaggcgaaaataaaactcggacacttgtatagccttaaaaaaataatt taaagatttttatcatctctgattaagccaaagaggtaggaatatggttgtatgttgcca tgctaacacatgcatgtagtgtgaacagcatatatcattctgatttcatatcacatgatt tcactgatttcatatcatttttataagaaaataccaacatgattgaaaattacactatta gcaatagttttatcatcattgtagataacctccaaaatgcttattttatatgaaaaggct tatttcttttcagaaggtagttcagagtaagatttactttttctgtggtagggctcattt tttccaacgttttggaatacctgaatattctttgcttgctatctaggatatttctcaatt ttaaagggactctgtagtattgatctataggaagaggcatttcagctttattttagtcag gatattttagagagttcattgtacaccgtttgcttgtttagctagcttatgctttttaaa taaaatgtccaaatgttaccgcatgctgtatttcatcaaatctgagacaccatagttttt ttagatgcattccaatttcggaaatgttaatgtgaaaaaaaaaaaaactgtattcaataa gctaaagaatgttagaatagcttttggtcctgtaaaaggagtaggtgcagtgaagaaaac atgttcttagttaacaggaatagcatgtggaattacccctgcaggtatagtattatatac atttttttcagataaatattctgtagcaaagaaatctgtagcaacaaaaggtcaactgga caggtagatcaggaccttcatcttttgagtaccatgtctgtctctgacacacccaaggcc tcattgttgtacactgccctttattttcagggctggtcccagagtaactagaaatgattc acttgcctaaaggcttactgtgctttaatgtctaacttgtagagatataatttcataata aagtggggagtcatgagttgacctatgtgagagagttgagggataattaaactggcatgt tttctacaaatatactgtgggggcatttcatgatgacttttctgttgcataataagcagt atgggagagcttgtccccttactgttctgctattgctcctaaggcagtgatacagcattg ctttggtccagttccatctattctgacaaagtactctgtattctgtttataaattgagta tgaacaaatgatattccattttgcataaaagaggactgaaacagaatgccactagaaaat agaagtgcaaattaaagacaaaaaccaaaaaaaaaaatagaaagtttataccatttgaat tttcagtagccattgatccactttgtatggcagtcagattgatgcttcagttaatttagg gaagcgagttggtcatttttttggcttgtttatatcattcaggtgctaataaatggtttt tcacttaaacttttcagactgggtaaagacattaattacatgaacattatcttatattga aatttgtattcagaagcatcttgagaatagcacaaatgacatagaaggacaagttcttag ttcaaattttgacattgtctcttaaaaacaataatttcattaatatataaccattcttgc ctttattttcacgctctataaaacacataacattaaaaagttgtcaaaggggaaaaaaca tgtacatctgcttaaagttatacttggaaatatattggagactcaataaatgttagtttc ttcccttggtcaagaaataatcttagtggacctcagaaattttctcagatctaaaaaggg accgttaacactagcttcacagtattgctatgaggatcaaagataaagtgatatggaaga actttaaccttacacagatgccattagctttacattattttaattaaagcatattggaat taagctatttatgtactttgaaaagatgttatactaaaaatatttttaaatgataagtat aaaatacatgtgttttgatattaaaaatggagaaaaaacttctccattggaagatttaat taggtttcatgggaacacttagaaattgtacatgttctcaaatctactttatttacatct tatttgctaatcaacaaaagattaaaacctacaggatagtgccaaaattgaatttattat tactttgaaattgtatttgggacacagtgatttaaaaaccttgttttgtgtgaaagactt gaagaaagtcatgtatttagctagagaagaccaggagaaaatgtgatgtctgtttttaac attttaacgacttttgtataaaagtaggaaaagattcattcttggtttcttcagaaagta aaacctgaattatgttctttgagtcagattttggcattaagttaaacttagcaattagaa ctggtccacagtagaatgggagataataagttactcgaatcctggaactgttcaaacaga ctggataaccatgtgctagaggtgctagagaagtgagttctttaccacatgaaattcagg tgcagtactacccaaggtcataaaacagtctatctaattgagacactaaagatcatccag accagtggttcccaaatgctgctttgtgagtcatctacatcaacactggatgagttgctt gtttaaaaaataaaatgtcttcagccactgaatcagactatctgctggcgggttttagaa ctcagtaattcttgctgtccttgatcggcatttgggaaccactggcctggaccaagcctc tcactgtgtaggtgggttctctgagactcagactcattaagagacttccatgttgttcta cagtgagtttcctggtgaagcccaggctcttgattgcagtttagtgctccttctactctt ctctactacctctgtgccctaatttacctgaaataaaacaccacttccatggggaatttt caaatttgcatttgcaattttgtccttaaattaaaatttgcttctgaacctggaatgttt ttcctctccctttcaatgtgttccaacatggctaccatgaaaggccaaaggaagtacttt ttttttttctcttttctgagacaaccacttttcaagtagcagtaaaataataattaaaag aattatccaacactctgcttttgcagataaactaaggcctgaagaggttaaattgtaacc taattacaacgacagtatctcctaaaattatttcctgatccccaattcagttatctctca actattcttcgttaattgttgttgtaggttgttatttcatgtacacatgcatgcgcgcgc acacacacacatacacacgtagatagtcattagctgcaaggttttcatgttaatgtatat gtcccttgctgctaaattgtatatatacaaaatttgaaaccactcttggagccagaataa gcaagcaagtcctagtgataccatcaaatataaagaatagaaacactctctagagataga gttgagatgatagcattaatctagagagagtatttcctgattcttttgggattcatttat ttattcagtaaacatttattaagcaccttttctgtgctaggccctacttgggtgtggtat gcaatagtgaataaaacagacaaaaaacactttttcctgaaacctacattctagtcagaa ggggatgaggtggatgagtttggaactggttgcaattttagagaggttgctcagggaagg aagtaaggaagcaaggcatgtgggtatctagggaaacaatattccaaggaacagcaagga aagatgctgtgaagctgagtgtgcctggagtgtttacagcacagaaagagtgtgtatggc agagcagagtaaataaaagggaaagtaatgggaaagtagtgtgttgtaggccaacctaag gactgatttctactttgggtgaaatggaaagccattagaaggttttaagaaggtttaaga ctttctgacagtagcactgagtacagtgtggagaatagaacgaagagccgaaggtagaaa gctggaagaccacttagggagttacaggaataatctaggtgacgcatactggtagctcaa tccagtgtagtaatagtcagattgtggatatattttaaagtttaaggctgtgagttcatt tggatcaaaagcatctgatggtgaaagagagggaggggtcaggaatgaccaaagacttct tggcctgagtaactagtaaaatagagtttattttactgaaatgaggaagactgtggaaat acagatttggagagaaagatcatagacatgttaagtttcaattacatatccaaaaataga tgttgaaaaagcaacaacataatacaggtctggagttcaggaaggactccaggctggaga gaggaaagctgtttttatggtccagatctttgtttagaatcaccaaagagatgtgatttt aaacgataggacagttgaatatgtgtagtcagtggaaacagtctcattttctattgctac tcgtctttatcagtggtatccttttttttttttttttttttttttgagatggagtttcgc tctgtcgcccaagctggagtgcagtggtacaatctcggctcactgcaagctcctcccggg ttcacgccattctcctacatcagcctcccaagtcgccgggactacaggcgcccgccacga tgcccagctaattgtttttatatttttagtagagatggggtttcaccatgttagccagga tggtctcgatctcctgacctcatgatctgcctgccttggcctcccagagtggtgggatta caggcatgagccactgcaccgggcctatcagtggtatactttttaataagataacacgaa gtaacatctgctgagtatatagataatttaaattgagacgttttaaattgtctctggttc ttattaaagattctttaaaacaaaataaaaatacattgtttcaaatattgtttaaagtct tagatatgttttgttatacaggcataatttttttccatgtactttttcttaaatatatgg aaatttctaacacagtttcaaatatttagcttaggtctgaattagttgtttatcatctgt tcatctgttctgtcttctttaatttctacaccctatcctccaaaagccttgataaaagat atgtctcatatattagctttttttttttttttttttttgagacaaagcctcgctctgtca cctaggctggagtgcagtggcatgatcatagctcgttgcagacttgacctcctgccacag ccccctgagtagctgggactacaggcgcattccaccatgcctggctgatttctaattttt ttgtagagatggggtctcgttatgttgccaggctgatctcaaattcctgagctccagtga tcctcttgcctctgcctcccaaaactactgagattacaggcatgagccaacacacctggc ctaagctacattttaatcaagtcagcatagttatccatcactccaaatgagcatgtcctt gtattagtgtccatgcattgtctatgatgtagatggagaggtatagcatattatcaaaac ccacctcaaatcctgtgagggatgtgaatcaataaattaatgagtttgttctattgatac caaatggagagagatgagtggagattgatcactatcagttatgtcaaagaggtaagtttt aaaccagtgtttgaagttatgtgagagaaaccaaaccaaacaaggtaattaaacttcaaa ttaactgcttttaattggtcacgtgtgtacatatacatgtttatgagtattttacatttc tttgtataataaatcccctgaccaaaaaagatacaatgtaagactcaaggtgaatgttct ggatacatgtatttgtagaagcaaaaggaagggacacaactggttggaacctgtatatct ttaacattaggtggtaaaatggaatatactatggaaataaataccattgaaatgttttat ggtattgccaatttaatacatattttatgataagaaagaagagaagaagttcattgtaga ttccacatgggtaaatactttaaaaagtattccaaatacagctcctcatatccttaatat atctgttgtcaattattatatatttcatgtcttggatcaagttttcttgtttggacaaga aatggaatggcaaaatccagatctttttagctgaaaacctcacctattctctattcaccc acttctaaagtggacacaataattcttaaaatggtacttaaatcagtatagatttgcaga aacaaaggcctgcttaagaatgttctttcatttaagtattatgaggttaattaaatgtgt gaaattgtaacattttgaattattcacatggcattttagtttttccttttaagaaccatg gcttttctgtgcaggtgttcttcactgggataatgttttaagataatcccctgatgtcat ttaaaggaactcttatttctgaagcatgattgtgtgtttttgtgtttttatagtgtatct ctttcttatcctcttttggcctttgtttatattctcattttaccactatcagctactatt ttctattgttgattgtttttattgtttattctattttctattgtgtatttgttttcacat aacaaatcaaagcctgtttaatttgtggaagacagttgtcaaatgtgtaatatatccagt tactaatttttctctgattgatgctgctcgtctcctttcagatgagtgccttattaaaac catctaattttatggtgcttactagaaagaatgtagagaaattgtcaaaattttctatta tttactcagtaacccaaaatgatgtttctggaataaaattttggcttctcagttagtgac ttggtggtagatgaacatataactattataataatgtaatgaaaaaatatttttctgtca aacatgtttttttttaaccttttacgaaataaaagttgttggtaagtgatgaaattaaat gccttcttcaatgccttgtggaaaaaatgtcactataaggagccactttttatacagttc taccctgatcaactatggttgtgtgtagatgtgagttttcctttataagcctgtaatttg ggacatctgttaaaacaataaaaatttctaattctctttgatagagctggagtggaccag agaaattgcttgctttagatgaactcattgatagttgtgaaccaacacaagtaaaacata tgatgcaagtgatagaaccccagtttcaacgagacttcatttcattgctccctaaagagg taaagattatcaattacattcttcattgggtgttatttgaatatataactcaacagctac cacctttaaacattgtcatgctaattatggtattcggtattgtcagtttgacactcttaa ttagtggtaaagagggctgtaaattgataaattgccatgacttaataatgtaattgtcag attaaagaatgcatgtgaactaagtaactcctttccactatttgaataagtcatatatgt gagagtaactattgagtttcactaatttgattatttatttaccaagatttttttgtcaag ttatttgaagcccaacctttagttatcttggtatattcatgatagtagattttttaaaat tctgaccatattaaaatagcaatattttttctcttattttattatatatcttaagtccct tcaagaccaataggtttatgattctattgaattctagttctctggtacttaacattgtgt aattattttaaatacaaatattataatttcacatgtgtatatttttctaatcattctcct ccccatctccattacacaaatacagatttcccatactaattacatgctaacagcacttct ttgggaattatttgtgcctatttctttttatatttttatgtaagctgaaactatcataga ctgattatatgggcattcataactataaggcatagtttagatatagttcctttattgata ttatagacctagtaattttgtcagtgaacagaaataatataacattaaataatcagcatc accatgatttatattttcataagtaatatgtatatgcccctagttatatgtcagctaaga agacaatataaaatactaacataccatttcaaatgatcctgaaacttcaaatttgaacag aaaaaagtcatttttttaagattatcattctaaatatcctaatggtgttaaagatagtcc caattagaattgcacgttggatacgaataaaatagttgtatcatatttaatgcatggaac taagcataactccagagaaaagcaaagtggagaaaatcttttcttcctaaaatgtgtttt ctactgagtgctttttaaaaatgactttggcccagaagaaaatttttatttttgctggct cattctaaaactgaatcctttgaatagatttggcttatgaaagtgacgtcagctctcttt gcttccagaagggcattaataatgggaaacacttacataagtatttgactgaaagtgatt ttattcacctttgatatattctctgtagtttacttagtgatatcctccgtattttagagg ttatcatttttgttaaaatgcaggctttctcatagtctcattttttctcttatttctaat aatggtgaggtttttggtttgttttgctttatttgaatttccataacttcttaacagaca aaatgttgtgtaagatcagaatatgaaagtattttgaaaaatctaagaattaaaaacaaa taatcaataaccagggtaaatccagtaaagattctctcagtttaagtgttcttttaaaga ctgcctggggaaaaataagtgaaattagttatataggagactaagggagagccttggttt agcatatcatctgtgtttccctcttgttttcaactaccagaaaaaaaaattcctattttg aaaaatgctttattgtctaagtggataaataatgatatagaaaacattatcttctcagaa acaaagtattattatttaaaacattattaccttcttgtcaggaaatggacttatttttct tataattcctttggccatatctcaaaactatttacaaacatttactttattaatctcaaa aagcttgacagaagtctcactaatttcacactagaggcttgtgatgaatagcattgtctt tataagtgaataccaagaaaatcataatcatacaatagatgatctagagcaaaacctgaa attcttaatagaggattagccaacatttaaaaataattttttttccttgactagtttaat ccactgaaatacagcaagtctgggaaaatattggctaccttttaagtacaatgtggttgt cttttatataggttatagagaaaaataaaagcaattcttacagagttggacaaggaaaag gtaattatctgaaatacgtttcaattcaagagcttttctctggcagaagtgatctttagg aagaaatactaatctgggttttacctttaaaacaaatgaatagaaaactgcattaactgg accatttgacttggctgtaagaaacccataactccctaccatagcggttggacagacaag agtctcactctgttgcccaggctgggatgcagtggaatgatcatagctcactgcagcctc aaactcctaggctcaaatgattctcctatgttagcctcttgagtagctaggactacaggc atgtgccaccacacttgggtaatttttttgcggggtgggggtggtggtagggggggatgt cccttttatgagagtactaattccattcatgaggactccaacctcatgacctaattattt tccaaacacctcacttcctaataccatcacattgagggttaggatttcaacatgaatttg agggaatatggacattcagttcattttagagccctacatgacaaaaccttgcttcctttg agggaactggtatacttcctcctcataatacgtagtaagaatcatgcaactaattgctat tctctttttggcttggcagtttaattcaaaaccttgtaagacataaccttgttgacagcg tatttgtgctctactttttctctagtttaaaccttcttttctgattttttaattcatatt tttttgttttgctttgtgtattttctgtattgcttagatgttttgatggaactggctaga tgtaaataggcaaacatttaataagtcgaagttcttggttgagttagtatcttcactact tttaaggaaagtgtgccagtaacctgtagagttagcacttcaactttctttagcgcctta gtatttctttatatttctctttaatgcactggactagatgatctcataatcactattaat gctggaaattttacctggtgatttggggatgtacttttgaaatctaagctgctctggatt tgctgtttcctagtctttaagatacattgactatttctgtaaattacctgatttgtactt cagttagataacgaaagcaaagaaaagaatagactttttctgtttttagataaatatttt ggtcattaattagcatctcttttgcacaaatatttttatgcctgatagcttctatgttaa attacaattccacatagattagtaatggtaacaccacattactgctatactgacacaaag cgaacttgcctaggctttgattgattggtaattcccaaattaagtagcttttggagtatc tgttaagtacaatattgagactatatatgtgtgagtattgttgaatatagttacaccttt ttattttaaatgactcagcttctctacataatactggtcttctctgatataccaaaagta attatttcacaaaaagtttttcactatcattcatcagtagtagtattaaactgaatattg gctggttggagtattttgtatattgaatatactcaatacatatgtaattaatgatgagtt gttgttgttgttgttgttgttgttgttgagacagaatcttggtctgtcgcccgggctgga gtgcaacgacgtgatcttggctcactgcaacctccacctgctgggttcaagtgtttctta tgcctcagcctcctgagtagctgggattataagcatgcaccaccacacctggctaatttt tgtatttttagtagagatggggtttcaccatgttgaccaagctgatgtcgaactcctggc ctcaagtgatccacccacctcggcctcccaaagtgctggtattacaggagtgagccacca cacccagcccatgatgaattccttatatatgcaaagtcattgggaaagcataatttatat tatatactttacatttagcatcaaaaaataattaaaattgtgcttttactccctgtgtga cccttgaccctttaacggataatttatgttagttcttgtcatttatttcatatgaaaact gaacttaattgctctatttaaattgttttccctagtatcataatttaaaattttatgtga tatgtgaggctacttgaataatcagtcagaggctataattccccttctttcttttttttt ctctttatttttcctttttatttatttgaactgacacactgaaaaaaaatcaatagttta taagtattataaacaaagtttttaaatcagtggtataccaaagatttagctcaaatatac aactcttatgtggcccatcaagaagatccttataagtaatataaggtaatcagaactcaa ttacttttatatgtctatggaatggtttaataattgtccccctttgtcatttacagtcaa ggatgtaagtgtttcagaagttcattacaataataaacactttgatttgaagactgttaa ttatgatatatatctatcaaaaatgatctggagtcataccacacagaatatatacaaatc tcatttcttatctgtcagggaagcatttaggtcctatcttcagtgaatttacattgtagt aggatagagaaaaaagttaaacaactaataacattttttttaaaaaattgagttgaggct cacagagtaaaggcaagagcagagacagagaggtttaataagtagtccttgaagagggta aagagttagtagagtgaagttcataaaaaccataaggtgtgtgtgtgttttttatctggt atatatttgttaggactgaagttactgcctttcataattttagaaatcagagtgcagaat tatatttatcaagtatctcatcctgtggagaataattaacttattatataatatatatat ttatttaaatatgggtattatactatgcatttttttcttagttggcactctatgtgcttt cattcctggaacccaaagacctgctacaagcagctcagacatgtcgctactggagaattt tggctgaagacaaccttctctggagagagaaatgcaaagaagagggtaaggttcaaaatt gcatagagaatatgtttatactttgaaaagcagagtgattctgaaaacaagtagaaaaaa taaaaataatattcagctacttatgataaaatccacagattattctacacattcagatga gccgaatcttaacagctaacccctttaacaagcaatataactaatagcaaatatatctga tttgcttcctatcactagtagaattaataaacaatgaaaaagtcaagatgaggatgactt aaaggaaaccccgaaattatatttccaattaaatatccaaatatttagtattaattcatt cttttcataaatgtttattgaacacctcctatgagccagacatttcttcaaggtggggga tacagcagtgaactgaagttctcttttgtcatggagcttatgttcttgaaacctgttttt ttccttagtagaggagtagttatcctgtgtattgggagaactgttttttttgtttttgtt ttttttcccagcagacatccagaaaaatatttttgaaaaaatataaaaatactatgtgcc tcagaagcaaaatattttagaaagaatcatagagcatgatgtaggtactctataggtgat tattcccttcatggtagttatagtcaagtaggaagcattgatggttagtccagttaataa aggtttgaggtaaagatccagggtgcttggtttgtcatcctgcttttgacctctgtaggg cttggattaatcagtgaaatcttcattcttaagtatttgcatacataaagtggaattaat ctgtgccttcccttcttccatataatcattttttaaatgatcttttaaaaattgtgataa catgtatgtaatttatcagtttaaccatttttatatgtacagttcagtggcattaagtac attcacattgttttgcaactaccatctgtgttcagattcactaccatctgtgttcagaac ttcatcatcttcacatgaaactccatgccccctgttctaactccccattattcctctcca cagcctctggcaaccacccttttactttctgtctctatgagtttgactactctaggtacc tcatataagtgaaatcatacaatatttttccttttgtaatcagcttatttcacttagcat aatgtcttcaaggttcatccatgtcatagcatgtgtcagaatttcctttattccattgtg tatatgtatctaatgctacattttgtttatccatctttcgatggacccttggattgcttg cacgttttagttactgtgaataatcctgctatgagcatgggtgtacagatgactgtttga gactctgcttttaattgttttggttatgtatccagaagtggaattgctagatcatatgtt aattctgtgtttgttttggggaaccaccagactattttccacagcagctgcaccatttgc acaagcattccaatttcctcacatcttcaccaacatattttagaatcattttgagaaatg aaatagtgaatacaaaaatatgccatatagaatgtaaactttacattattgctacatact gtgataacagtattggaaatttgacaatgtcttgcatttcagtggttttgagcagagaga tggaatactctttataaagatggaatactttataaaatgtataactgttattattggtga aggcaatttactcttgaactgtaacactttagggattgatgaaccattgcacatcaagag aagaaaagtaataaaaccaggtttcatacacagtccatggaaaagtgcatacatcagaca gcacagaattgatactaactggaggcgaggagaactcaaatctcctaaggtaactgaact ctgcacaatgtaacagaaaccgttaatcagtgttagtacctagtgtactatttcacttgg caaactgtataatatgacactcctcttctgtagaaagaaaaccgtatattttaaaataac tgaaagtattaactgacaatattcttttattgctaatttctctaatctgacaactaattg tgcttgaataaaagataagttaaattgagtatctttttatcttacgtaaatgaaaatctc tcaatatcctattgaattttactgtgtcatacacattctacctaagaatttgctgcctcc tctgatcacatactccaaacatgattttttgaggagtgtctgtgttttggatttggtgac ttttgtgtgtcttatttttacaaattgaagcataattctggttttctgtctaataacatt tcaaatacataaagaatggtaaaaacacttgaaattattgggcgtatttttacaactgga aaaaaccaatttggggagacattgttatactaatatgtatatattatgattttttttgtt ttaagaatgctaataattgttactctttgtggttaatagatgcttattttgttcttgtgt gtcaaagttgtaaaaattagaggcatattcttctggtttgaacagatggaatagattatt gtcaaatacattggtgtttgaaccatctttatgaggtcttgatgcagtaaaactttaata gttgatgttttgagtataaataatagactatggaaacaataaaatgtttaattctggatt cgtcagccaggaaaaataatgctttatataaatttaaattctagagatagactacaaatt actgttcctgtttatgccttcatttttctcttcaccagtattttaaagaacataatttaa ttaacatatttctaatctgcacatctttcttataggtgctgaaaggacatgatgatcatg tgatcacatgcttacagttttgtggtaaccgaatagttagtggttctgatgacaacactt taaaagtttggtcagcagtcacaggcaaagtaagtttactcttcctacacttcacaaagt cataattttgtgtatatcattgcaagtagcaactggaaaaaagaaaacaataattcattt ttgattcttagttatttggatagcaaatatcagagctcataacttagcttttttccccat atttatgaaagccaatggcatgaggatagtgatgcttctcatgatagaggtttcattaat tttttttcatttacatagtttgtctatctttgtgacagatactttaacattttcaagggt gctttatgtttttcttggctcaatttttccccacttttatttactatctaagttatacct cattactttgtaatgtttttcaaaagtatggttgagggatatttatttttccttataaac tgctttgttgataacctacctgtagtttagaacttaataaatcatgttaatttatactta tctcaaacccttgtgagatgagagtggacaaggcatttaaatgttaagtttgaagaaaaa gcccaagcaatcagcttgactgtgtaccatatcataccttaatgattctctagtgaatat ttatttttatctgtccagtagagaatattttcatgaattaagtaattgccacctaaaaaa taacagaagagagtatgaaaacaaaaaaaaatccatgtcttttaaaatgtcttttaccat gttgcttcttggcaagtatcttataaatattccaagtcagatgttaaaattttttaaagc agctttatatttccatagtgtaatattagtttgggatttattacagggctttaatgtctg tctttacttgtctagaagtagcttcactggctcctaactactgccctggggcagattcag atgaaatgtggtatgtagccacacccaatactgttggcatactttcattgaatctattcc catggaagaaacccagttgcaatttcagtattatttcatcaaagaggccaggccagagct atcataactgctttcatgtcgtgtttcctttttattatatcatgttttatatcatatgtt tgctttctacatactacatacacaattgcttgtgcttatataaatttataatgcttttga ataaacaaaacaataaatagtgatgggatcattttatacggatgtaatattttctgaaga gccaaacaaattaaataagtaactttactccaaattttaatgagctattaaattttattt aaatcacttttcctttctacccaaaagtaatcatcttaagtgtttttccagtgtctgaga acattagtgggacatacaggtggagtatggtcatcacaaatgagagacaacatcatcatt agtggatctacagatcggacactcaaagtgtggaatgcagagactggagaatgtatacac accttatatgggcatacttccactgtgcgttgtatgcatcttcatgaaaaaaggtaaggg aaaatcttgtcatggttgggacttcttcctctattattggtgaatttgggccctttctgt gtagagttactgaaatagtgacagtgtgctcttaaattagatttaattattacacttcag atgtttctattctaggaaagccatagcgttttgtcttaaaattggtgattgtctgtaaat ttcctttctcatttgctagtgttgtcagcttcctgtttgaaactctctactctctaatct ctaatcctttttgttgttctgtatctttgatgactcatcatattcaagcagaaatcatgt tttctctctctttttttgatattcttatttccgcaagtgggacaaattcaggcttcaagc gtctgagcctttttaaattacctacccaccttacacccaccctatctctaagtctaggca aaagctgaggcctgtgggtaatttgttctctcattctgaactattgtcacagctttctaa cttggttatcttatgttttatacagctgacatatagtccttttataattatgatagtgtc actgcccagttcttaagtctttttgagcctactgttgactaacgaattaaggaagaaatc ctcgatccagttttcaaaatggtttctgccaaactggactttttgactttctcttctcag tgcctttgctgttgcttttatctcctcctgtaaagccctcctattaaaatcctatctttc tattatcgtcatcatacccctgttaaacaagtgtgatttctacctcatttgtcaaaaaag gtctctctgaaaggtccttacagtgaatccatggcagtctttacctttaattctgtctat cgcatcttctttattaagaacaattacattaagcagatttcacaccagatttgtaatatg tgaaaaggagtaccttttcaattgtcatttacctctactttgaacagtgtgctatactgt taaaatttgggggatgagtatcaagagaccttgtattccagtttccattatgttctctca acattaggttctcaattaaagggttctctcaaccctttaattatctacttttttaaaata tggaaactgtggtatttctttatctcttcagaatgacataaggatacctgagttttcagc tttgttttgtaatccaaaagtgattacagtatttgtatattgctctggttttcagaagag tatagcgctgatgttgaaccaaatgtttaagggatagccattgtttttaaagatgtacat atcttttaaaatctttatctcttaatatttttatagcctattgcatatttataaatctaa tcattaaaagcataagcagcctgtgtttgaggacatgtctaaaactggttaatttttact ggaatgtatagtgtaaacacaaatctgcagctgtttaaattccaaattctgacttgtttt gttgtataaactttattgaattttttacctaatcacaagggaatatatgcattcataatt gaaattttggaaaatggcaaagaacataaagaaaatttaaattctttaaaccactaccac ctagagttctcccattaacattttatacatatgcccacattaacattttatttctaaaat caaaatcttattgtatatatttagaggcagcttctgctgctttttaaaaaatacagtaca gtggttttcaaactcagtcccacagaaccttaaggtgctgaacctaaggtccccaggtca gggaaccaagcatgcagcattctaggcttcccaacttcccattcccttattatgtttata taccaaggttttacataggatttcttttgaaaatggttgttgctgtgtaaaaaaaaaaaa aaaaaagctaaaaaaccttgactaaatctaccatgttttctcatattattaaaaattcta aacgtgggtttttttgttttgttttgtttttctgtttctccctctgcagagttgttagcg gttctcgagatgccactcttagggtttgggatattgagacaggccagtgtttacatgttt tgatgggtcatgttgcagcagtccgctgtgttcaatatgatggcaggagggttgttagtg gagcatatgattttatggtaaaggtgtgggatccagagactgaaacctgtctacacacgt tgcaggggcatactaatagagtctattcattacaggtaagatctcttatctctcccttaa atgctctcctgatgaatcataaggttgttttactcagataatcactgtcaaattgctgat ccagtacagtccaaaaaagattaatcagattattctgctgtttttatatacgttaaaatt ggcatgaagaaagtgtaaatttcaaaattatttaaatgctttcaattttctgtgatctat ttctcctctaaatacaaatatttgttttgcaacctgacactttggggctctaagttttgc tatgttaattaatggttaaatagtctcataggagagtaacctggattttaagaaactcct ttgcagaagattaatatggccaaaagctgcccctttgctttgatagctgaaagttgatgg accatgtaattgtgattccatccccattcaaagtggaaatagatcctttctgcataaagc actctactgtcccaggcccactgatttgtagtcataaagaagtaaagttgactaaaccga tttcttttttttggtttatatttcaagcccatatccttgaaaacattgccatattttgag atcattaactagatgaataggaagtttagaatatgaatcttgttttatttccagaaaggc aacatttttaatgtaaaaatatttcctatagcttatatagtttatttaccagttattacc cattaatgttcagcaaattagtacataatcttagtactgtcaattctgttcttaggatat ttgtttctttgtcaaagggatattgtccatgtcatgaaatattgacaatatgataaaaat gtttgctaaattagaaaaggtgatgaatttggaggaaggggaattggctgcacctgtttc tgatatgttcagaagcttaatgaatataatattctaatttaaataaactgtttgattgag aaaagaggtagccacattattgtttagaaatgatagactgttattgacttttggtgtagc tgggaagctggagaagaggtagtatgtagtttgcttttgatttcaaaatgccacctcttc tgattccagatacaattatcttttggcacatttcctaattagcattaggttcttataaat gaaattttattttacacacagtttttaatggaacttacttttgaacatcacgaaagttat ctctagcccttttcatgccttaggtgctgataagcattccgtttatcataagctatgtca ttagtctcagcttcctagtgggaagtaaaactcatagcaattctctcagtcatccatgat atatagctaggtggggccagatgatttgaaaattaacatattgttcattttaggtgcttt gttttcattttaagttgtttctgcatctagtttgaagctgcttggcataaagatgagcct ttctgatagaagtgtgtgagaacatacattgtagagttgcttgatggcatgcactttatc ctcattgccactttatcttagtacctcattttgttcctggcattacctgtttcccatcct cttccccctttcctactaggattaaggtcagtaattgataggaagagtatccatacttct attctaataaactgtgtcttttgtagtttgatggtatccatgtggtgagtggatctcttg atacatcaatccgtgtttgggatgtggagacagggaattgcattcacacgttaacagggc accagtcgttaacaagtggaatggaactcaaagacaatattcttgtctctgggaatgcag attctacagttaaaatctgggatatcaaaacaggacagtgtttacaaacattgcaaggta agtcttactcacctttcgttttgttcattaaaaatgagatcattattcaagagaaatatg gagccttagcatcacaaaaagagtcaacctcatacagaatggttacttcagaaaagttta tgaatattttcaccctgacttacagaactagtctactcttgaggaaatgttttaaactgt gtttttaatatgtaatataaaattgtttaatcacttcctgcatgttcaattgactctact taacctttaagaacagtgagaagaaagatcttggctatatcatagaagcaacgtactcat tatattttttgattgaccattgcattttgaatttgtagtccaattttaaagggctacctt attgaaaaaaacaacagcatccttgatagaaaatttatttggcctccacttagtcacgtg cttaactggaatggagaggaataatagtgcctactaaagtagagaaagaaacaagtgaaa atcactaatattagatgaggtacagcagtgcctcatgattaactttttctatttgttata gcccttagaaagtgtttttttcagtggggccatctgataggcaacattgcagctttttac tcattggttttgtctctcctcagagttttggaatagtgactgttcagctttgagtggttc atggtggtggggtaatagatctcttagtgctcatttgtccatcagcagaaggtatgaagt tgcactatctacatcctcaccaaggaatgctgtcgttcacacagccagaaaataagatgt ttgtctggtagctaaccaagcttcttggaagacagacctcttgaaagttaatgtttcagt tacaaatcactaccagattttcttacatccatgctgatcctaaaatactgaggacatggg tttctaaatatgtatcatttcaaatgttgcatttattgtatgttctaaagtagaagtcta caaattataatgtaactaactcatagccattatttctaaccagtaattaaattcttttgg tttttgtctaggtcccaacaagcatcagagtgctgtgacctgtttacagttcaacaagaa ctttgtaattaccagctcagatgatggaactgtaaaactatgggacttgaaaacgggtga atttattcgaaacctagtcacattggagagtggggggagtgggggagttgtgtggcggat cagagcctcaaacacaaagctggtgtgtgcagttgggagtcggaatgggactgaagaaac caagctgctggtgctggactttgatgtggacatgaagtgaagagcagaaaagatgaattt gtccaattgtgtagacgatatactccctgcccttccccctgcaaaaagaaaaaaagaaaa gaaaaagaaaaaaatcccttgttctcagtggtgcaggatgttggcttggggcaacagatt gaaaagacctacagactaagaaggaaaagaagaagagatgacaaaccataactgacaaga gaggcgtctgctgtctcatcacataaaaggcttcacttttgactgagggcagctttgcaa aatgagactttctaaatcaaaccaggtgcaattatttctttattttcttctccagtggtc attgggcagtgttaatgctgaaacatcattacagattctgctagcctgttcttttaccac tgacagctagacacctagaaaggaactgcaataatatcaaaacaagtactggttgacttt ctaattagagagcatctgcaacaaaaagtcatttttctggagtggaaaagcttaaaaaaa ttactgtgaattgtttttgtacagttatcatgaaaagcttttttttttttttttttgcca accattgccaatgtcaatcaatcacagtattagcctctgttaatctatttactgttgctt ccatatacattcttcaatgcatatgttgctcaaaggtggcaagttgtcctgggttctgtg agtcctgagatggatttaattcttgatgctggtgctagaagtaggtcttcaaatatggga ttgttgtcccaaccctgtactgtactcccagtggccaaacttatttatgctgctaaatga aagaaagaaaaaagcaaattatttttttttattttttttctgctgtgacgttttagtccc agactgaattccaaatttgctctagtttggttatggaaaaaagactttttgccactgaaa cttgagccatctgtgcctctaagaggctgagaatggaagagtttcagataataaagagtg aagtttgcctgcaagtaaagaattgagagtgtgtgcaaagcttattttcttttatctggg caaaaattaaaacacattccttggaacagagctattacttgcctgttctgtggagaaact tttctttttgagggctgtggtgaatggatgaacgtacatcgtaaaactgacaaaatattt taaaaatatataaaacacaaaattaaaataaagttgctggtcagtcttagtgttttacag tatttgggaaaacaactgttacagttttattgctctgagtaactgacaaagcagaaacta ttcagtttttgtagtaaaggcgtcacatgcaaacaaacaaaatgaatgaaacagtcaaat ggtttgcctcattctccaagagccacaactcaagctgaactgtgaaagtggtttaacact gtatcctaggcgatcttttttcctccttctgtttatttttttgtttgttttatttatagt ctgatttaaaacaatcagattcaagttggttaattttagttatgtaacaacctgacatga tggaggaaaacaacctttaaagggattgtgtctatggtttgattcacttagaaattttat tttcttataacttaagtgcaataaaatgtgttttttcatgtta

TABLE 2b Mouse Fbw7 nucleotide sequence Mouse (mus musculus) Fbw7 nucleotide sequence NCBI Reference Sequence: NC_000069.6 (SEQ ID NO: 4) gagcgagcggggccgccaccgccgcctctatcccagcagcgcggaagagacccgggtagc tgcttggtggagcgacgctagcaccgcttcttcctcagttccgcgcctagccagccttcc gcagctgcccgcctcagcccgaaggaggaagggagccagccacattgtcagcgccaccgc tcgactctgaagcgtgagtggacccgggggagggggacattataaggtgacaagtgggag cgggtgggtgctttgatgctggagttcggctgttcctcgggagggttggcagtggaaacc tttcctttctaccaataggagaaagtgtcagtttgcaggagtaatgcaagtactcggagt tttttcttagagcagctgtgtctccagagtggacacttgcacctttttggacactaaact gagaaagcctatggccagctctgtcccatgccttttcctggcaaccttattctggcagtg gtttttgtttcagggaaactcccatggattcccacgtgggaatcacttgaatcaaccctt aatttttttttttcctttcctaactattgtttttgaagttgccttggatgggaagaccag atttcattttagtgcataggggagtttggtgcccccaacgctttatttcccaggtctaca gagtccgatgtgtagggtgcttgcatgtttcagttggctcaggaaaatccgagttcctaa gatgcacaacccactgaaacttatttcagggagagcacttaataaattaccatttcgtaa aagttgctgcagcgctctctctctctctctctctctctctctctctctctctgtgttgag ggagagagggagtaatatgttttaagtggtagcacatttttactaggtttctactaggga ctggaagggcatgcatgcagctgtttttatttggctctacttgtcgaccttgagacttta tactcttttaggaaaacatttagcactctgagtgttaaattagaggtatagaaaagtaat gctcacatatgtaggttaagctgaccatcctaaggcttaacgtactgtaggatttcatgt agcaaggaacataggtagtagtttaagactggaattctggtctatccagacagctaaatg tgggcagaataaagatgctctgtagaaatgactagaaacttgggagtatcaatttatcta cccatccatccatcttaatttctccattgagggtcatgattaaatttgtttgaaagtttt tcacatttcatgtttgggacctgttctcccctcctctttttaaataatgaacctccaagt ttatcattctgcattatatagcaatgtatttctctttgaccagccttctcaatctttcag gatacagtttgtttgaggaatgctaaaacactgtgaatttaccttaccttgcttttatag attttgtttaatctctactatcatctgtcccaacttttcttttcttcaagactgtttttc tatgttaccttggctgtcctggaactatctctgtatatcaggctggcctagtgttcacag atatgcacctgcctctgcctcctgagtgctgactgggattaaaaggtatgtgccctgact gccagccacgtcacagcttttttgagtagctccctcccccaaccctcatttctttggata actttgccctctttttggatctcctagccttttttttttttttttttttttgttagatac tttgttcttcatatgatttcatgagacactttaaattttgcttaagtgggaattagtatg aaaatattgaatggtaaatttaactttagatttagaataaaacgtgtttttggatctgct aaccttactttgctaccctgataaagtcatatattccagttttgaaaaagatgatatttg agaaacttagactttaatagtttgcttcttggaaaggaggagagtggtactttgagtttg tactgcatctaatcagaggacctgctttgtgtgtaaattggttttaaattatggaagtac tttactgaatgtagtaataacaagacatttgctattttcagtcttttgagggtgacagag acaaccaactacaaattgcacaaaactttaggaaaggttgaactggtgggaaggctctgt gggtaaagctgcttgttgcaagtctaaggacgtggagtctgtctaatgcccagaaccaca ggtggacagagaaactaaccatacagagttgttctctgacctgtgtcgtatgtgtggaca tgtggtggtagtacagttgtagtagtagtagcagtagtagtagcagtagtagtagcagta gtagtagtagtagtagtagtagtagtagtagtagtgtagtagcagtaaaaagaaatacat ttcttaaaaatgtaggaaaggggacatatattaccattattactcataagatcactctgt gtcagggaatgcagttgcacatttttattagggaaatactgattttggatgacccaggaa taattggagtgtctgagccaaatcttggaaaggctctttcaggaagaaaagaacatacac aaacaaaatgttaagggcttagcgtcagaataagtaaaagatgagatctaattaggaagt tagcttggtaggaatgcctactgagggagggaagttcctgactagagttaaggtcagttt gtgcttgtataagagaatttgaaaacagtgttccaaagaaggtagtttcaggatattggg agaaattatattttaggacagacattatactggctttaggattttgtttcattaggagac taacatttttatattatggtcatctagggggcagcacagaaggagacacgaaaaaggaaa tgttttgaatgttttttagcatgatgctagtttcttatgagtaaatcagaaacagggtag caggctttgctttggatgttgaatttggggttaagagctaggtatctgtacagttcatgt gttagctcattgtttttcttttcctccctctctctttaaaactttttccactttctttct cttcttttgtctctgtctcttgtgccaggctgaccttgaacttgttgtgtaccttttaac ttgtaaccaccctgcttggacttcctgaatgctaaatgtactagtgtgtgctgccatttc ttgttttgtagtgctggggatggaacctaggcctttgtgcatgctaggcgggtactttac caactgagctacatccttaggacctaccccgtgcagctttcaagactgcaaaatagtttg agtaatatgctgacagtgtaggtagatgggagtcatttttaagcctttatatttctgaga ttactgatacgaaattaagagctgaaaaatgaatttaaaattttgggacctgaagcagat ttgtgttggtataagcacagctatcaaaaggagagacagtccccagaagatcagcttcgc aggtgagtgggaatgacagttggaaagaggagcagtctgtaacataatgaccattcagtc ccaggacatgggattctgttttagaagtagttaaaggttttcaatagcacatttagatcc tgagatagataagttcagaaaacataggaccttgttagggaacttataaataaaatggaa ggccattacagtttccctgcattttataatttttttccccgagacagggtttctctgtgt agccctggctgtcctggaactcactctgtagaccagactggcctcgaactcagaaatacg cctgcctctgcctcctgagtgctgggattaaaggtgtgcgccaccactgcctggctcatt attataatcttaagcttggtcttaatactaatgaagtggggctaaacattgatgaaaggg aaggctgtttctcttacttcagaaatgtgcatctttgtgtccctacaatgtgtgtttagg ggggacatccctgtgttggtacagaagtattagggatacttttttgtataaacttaaaaa gagcctacaatttaggaatatttaaacaacacaaacaaacaaacaaacccaagctagata atgatggcacatgccttttaatctgacccaggtagctctgagttcaaggccagcctagtc tacagagtgagttacaggacagccaaagcaacacagctaaacctggtcttgaaaaaaaca aacaaaacaacaacaacaaaacgaacaaaaaagactattcaggcatctttatggttagct tttggtattggctttgataaggataaaagattgtgaataaaaacacgtaatcattatgat cctttgtttgaattcattgtttttctttggcttcttaagagagcatccagttggcagtaa tctcttggaatagatatttttaaacaaggatgtgggtctacaattattttagggtgagta gatagggattggaatatctgcaatataggggtcttttgctacttggcccatttcttccat gggtgaagacatgcttaagtgtatttaaaagacagccttttaggattaacgtgatttact aatatggcgggattctgatggagaatggtaacatcaatattctttgccttgatagttatg ctatttatatgagaggctggaagttttgtatctttgtgtgtcttagtaccagaggactcc agagtctgtgtgagttatcagcagttcatttagatatactttgaaaatgattctttgaaa agtcttgtgggacgacgtaaagaatgggttggcaacctttctgtgtgggtcagcatactg tgtcacaactttcagctgtttctgccatgtgaaaacagctgtagataatatggaagtgtg ttataactgttccagtcagatctcatgcataaaaacaagaagtagatgggatttggcctg taggcggaggtttatagctccctggcagaggacagctaaatctaccagcatctactagtg taagctagttatactagaaagccagattggccatttcaggtggattttgttttctagatg agatgtgcttctctggactgccattgtaatgatgatttgcctttatagtaatgagaatta tggatcaagaagcagagacttgatgttctgtaaatagatctgaaaaatagagaccttcat cttaccagaaggtgccataattgatgtcttagttacttttctatttctgtcacaaatatg tgaccaaggcaacttactgaaaaaaaaaagcacttaattttgcttacagtttcagagggt gagcccttggtcatcattgtgggtgagcaggcataacactggagaagtagctgagagctc acatctgacccacaagcaggagagactgggtctggtgtgggcttttgaaactgaaagctt accccagtgacacacctccttgccttttcaaagcagttcaccaactggagaccattcaaa tatgtgagccagtgggggccattctcattcaaaccatcacattggtatgtagggatacac ttgttttgtattatggctaaaagaatctgttaaggaagaagttaactttctaccccactg ctacagggataggagccccgagaagctcgaggcctgctaatcaagcattctcccactgag ctgcatctctctatggtataagcttacttttgaaaggttctcaaaacaaagtataaagca tttgcttttaatgtgaaagtaaaaaggcatttaagttgttttgaatcagacaaagatgtt aagtcagactcaaggaaagttaaggtttaatgcttctacctcttaaaatcataatctcat actaaatcagtgtgaagaattcagtacagaattttcattttgttgttaaatcttaaatta cctaaaccacttgaatgtatactttaaacaacattgctatctttaagactaatatagaca aataaatgtactacctagaaagaactagctattcacttagtgaaatgtttaatgttggct taatgttaaaactttcgtttcatatgtcaacagaaacctgtattagatgctagtatattt accctggttttacaaactttgtttcatggtactgcaattttttttttttttttttgtata atacagtgcaatagtgatccctttttagttagtttcagaactgtattaactatagtgatc cctctgtataggacccctgcagataccaaaatctgaggatgtttaagtctcttatgtaaa ataaagaaatatttaaaggaattagtgagacagcttgacatcatgtctgagaacctgagt ttcatccctcagacatccctaccatggacatgatggaaggagataacttactcttgaaag ttgtcctctggccttcatatatgcagtgtatagtgtgccaaccctgccataaataatgta ataaaagtatttacatataacatgcttatatatataaacatatatatgtatatacacaca tgtatatctacatacctctttctgtgtactttaaaattcacctctaaattacttgtaata aatataataaagtgtttaataattgttataatacaataaagtagcacattagcagttgtt atgctgtgttgtggagggataacaacaacaacaacaacaaaaatctattaggttatactt ttttttctggatatttttcaatttggttggttgagtctgtagacatagaactcacatgga actgtttattctgtgtatatagtagatactttagtttctgttttgctctttgaataaaag atgatataatctgacaatacttttctttttgggggtggggtgtaggctctttagtttgta tgcatatgcaattctgtgagacagtctttcctgcccaggctgcttttcagtgtgagatac tgctgcctcatcttctggagtgttgagattatagggatctataactatgcctggttttat tttggtcttttaaaaatcttactttaaacattttgcttttaaaaataattttatcaaaag atgggtgatgaagttaaatgacactggcactctcatgtctgcagggatttcaagtagaaa tattttactttggcaacacttagtagaccaggaatggtgagctttttggtgtgtgttcat taccattgcaaatagatgagtacatttgttttcaaatagtgaactcttgccatcacatat ttctattttgagactcttctcatttagtataaaatcattctctggtaggcttgaaaatag acaattagatttttttgcagttttctgatgttacctcactgatagtttctgtaatacctt tcatgtatgcaaatggagtatgtttcctaagtgcatatgacatttatacatattctagta attacatgtttacatataaatatagttaaaattattcctaagacattttgagattgtgaa atccttgagaaacatttgcaattctcactgagccctgggacagtagaatgattaatttaa aagaaaagttttataaaatgttcctttacgttttttaagtacactcaatttctctaatag agtaagcttaaaaaagagaagatgagaagcaatatttgtgttgtatatcttccttgtgtg taggtggaaatgctcaagagatgagctattattatttgttctttctcctaaagcatagct gtgcttcagtcaggtaggtcctgtgtgctctgctgctaatttgtggcctctgccctttgc ttccacctgaagaagaacaactactagaaatttgggtggctttggaaagagctagttctt tttccctccctgtttgtgtatctgtgtactggtataatatgataaatactttttttctgt gctgtaagacttctctgatgtttgtaaatcttaaaagctagtgtgtaatcaggctaacac acttacactttaccttccctctgagaagtctctatgaacacactgtgtttaccttgggct ttctcagcccagactagcctggaactcactaagtcatttgtataggcctcaaacttgaga gccctttgtctcagcttcccaagtgctatcagtgctgggagtataggtctgtgccgccag agtccacttgccaatactggtaacaccaatcagtatgattatttacaattggttgattct tttaggatgtctgtttttttaggagtttaagaagagatagaacttggcatggtgttctag gtctatgaatttgctttaccaaaagtattgagcaatttataactaactacaatcagtggt tgtattcctggaaccagcaacaagttgctagctcatctctcatttacctacgaacacaca atcacactttgggggcagaatactcaggtaattgagaacccatttcttagcttcctcacc ttgaacaagctacttcctcagtacccactcactggggaaagcatgctctccacaaggttt gctgggagaagctcatatatatgatttaccaatgttaatttactaaaggacatgcctttt aatatgtaatgtaagtagaaggcccgagtcagccattattttgctcaacatttctgtttt acggtttctattgctgtgaagagacaccatgaccaaggcaactcttataaaggaaaacat ttaattggggctgccttacagttttaggggattagtctattatcctcatggtgggaaaca tagtgtgcaggcagacatggtgctggagaaggagctgagagttccacatctggatccata gaccacagaagtgagtcctacactaggcctagctttaacatttgagacctttaagcctgc tcacgtacatagtgacacacttcctctaaaaaggccacacatcctaatagtaacactcat tactgtgggccaaacaatcaaatacatgagtctatggggaccattcctattcagaccact aactacaattcacctttaacaccacccccgagacagggtttctctgcgtagtcttgactg tccaagaactagttctgtagaccaggctggcctcaagcctttgccaggattacaggtgtg tgccatctctgcctggctgattctatccaacatttaaatgagcacttgagcttgtagtcc tgtttcataagcatcaaaaatgaatatatgtttcattaacattttattaaaaagtcctaa aaactagggaaatgacagaactatttatagacaaagatttaatcaaatatcactggactt gtagttaactgccaaagacctcaagtggtgcatggaacagaaaggtttcctctttattct ggaacaattagtgttttccttttccttcttctgctagtgatgatgatttgagttgtccag gacaaatcctgggatgcgattcagttctcagttcactgataactttactgccaccactgc cccttcaacactggaaatacaaagctttggaaaatcgtaaagctggatgcaagatttatc tttctcagttattcagactaagattgaaataccttgatttagtgaacacattgttagttg atacctaattttattttcataaaatgattgtgtgtgtgtaagtaatgtgccttaaataca agatagctttttcttcaagtgtagaatagtcggttttaacagcatctacaaggaatagtt gcttcttaatggtttaaagagaggatttatgttattttaaattctgtgtatagatctgaa tgtctgtgcataggtgtgtgcacttatgcaggtgcctggaaaggccagaggtgtcagctc ctctggagctagagtttctggggattgtgagccacccagtgtgggtgctgggaacaaact ggggtcttcagaaagagcagtgtctgcttttaacatccaacccatctctgactcttgatt tttttttttttttttaatttggaaggtttttctggtttgaggaccacttggttatgcttt tacttacattttacttgacagaattatacatttaatttataaaagctcacaggcaggata aaagatggaaacatttcttagcagtgatgatgtttatgctttatgctccattcttcatgc tttattttctcctttgtcggcagctttcctttagattgtgatttaaatgcaaaatcaggt tgcttatctgcacgtggatctcttgttagattgtcaatttgatgactatctaatttttaa aaatttcaatttttaaacttttttctttttggtagcaattatagctaggtcatctctagt tttctttccacttcagttattttcaaaaaggatgctgatttctataagaacgcagtcacc tagccttctgtgtggtcagtagctctgagctgtgggtttgatgcaggagtgcagggcgtt tctctacggatcattacacagtggtgttgttttcattaaggaagataagattttaattta ctggattgagaaatttggagtaggaaacatttaaacttgttataaaattcattgtggggg agggggaggggtggacggtacagggaagggacagacagcaggacagaagagggagggctg aaaaataaactgggggagcctgtctccggaagttttgaggtcagtcttagtcagaggtca caccctgttatttctttgggctgtggacctggggtgttaccggttgttatctgctatact ttgaaatgttgattagaagattgatttttgttcttggcttttccacatctggattcatta aaccgttgaatttctaatcctttttacataatcagtgtgtcctcgggtggtagaatttcc actatacctgataagagaaggtttggacaaaaggtctgatctaccctacatttctgattt ctgtaagagggatgctaataattgtatataaggagctaagaggcaagctttgagctgctt gctcaagttcattgtgcttcctgtacaactgtaatgataataatagtacctacagcactt atggtattaggaagactggcattgcgcatgcgagcctttagcagtatctggctcagtgcc cactaagtgctagtcactgtagtaatacagagtgcagtgttacgttattgttgtatttgt gaagttgctctgttttcaaaaactgatgtcatttgtgatctttgcttttgaatatgttcc tattttgaatactgtctttattcagcagaagatatttactgaatacctactgtgtcaggc attgatctaagtgaacaaagcataaaaatacctgattgctgaaacttatctctggttgga ggaggcaggacacacacaataaagcaaaacagaaattgggaggcagaggcagttgaattt ctgagtttcaggccagcctggtctacagagtgtgttccaggacagtcagggctatacaga gaaaccctgtctcaaaaaaaaaaaccaaaaaaaaccaaaaaaaaaaaaaaaaaaaaaaga aagaaagaaagaaagaaattgggctggtagattttgtgtattggaaaccaccgttaatga aacctgctagtctttcccctgcttgtgttgctttgctttatttaggaagcacctgctggc ctgtccccagctgtctgtcttccgtggaaggtagcctcaaggctttcagcattgccttgg cttactgcttacggtggctagctcagcctttgtcttccagtcctccacctctgtttgagt cttggctttgcttctccccagtgtcccacctttggcctttcaactctgtcacactcaaga gacagttcacagaaaacctctttttaaatgtatttcatgtttaagcagcattttcccatt ttgtgtttcctcccttttcattttctttagctaggatagttagcttgcttgccagtctca tttaagaaatcctatttttagccgggcggtggtggcgcacgactttaatcccagcactcg ggaggcagaggcaggcggatttctgagttcgaggccagcctggtctgcagagtgagttcc aggacagccagggctacacagagaaaccctgtctcgaaaaaccaaaagaaaaaaaaaatc ctgttttttttttttttttttttgtatcgtcaattcagttgtgaatcttttaaggttaca gaccttttccattccgagctttcttaacttaggaagaaaggataatacttgctccaagga gtgttgtgagttttctgagagttatgtagccactcgggttgctgcagctctggccttagt gctcaagagcccttgtgtctgtcatcccgcacctagactaggagagcttacagtttcact tccattttcttaatgggtcacagttttaggtttcttttttttttttttttaaatgataca atgttacttttcaaaaatcacatgaaaatatgatgatgtaacaatgattataaagcagga attcaaggcccatcatgtgggtgctcacactgattgtaagacctttggcaagttcctttc tctctcttcagtgtatccatatgttagggaaactaatacctaccttacggatgcttttag gaaaaatgagatcacaacactatctggatgtagtaactaatctctcacagtggaggtgtt tctgatggtctcagctatgtggagagaagacaggaggagcagaggggacagtgtgggaga aggaggagaggaatgaattaactcactcactgctgggatctaatgactatagaaactgct ataaatgatatatagggcactattcaaagcaaaaaattatctggtttaaaataccattaa tgccagtatcgggaagcctgctttgtaattaagtccccactctttcatttaggtcagttt tttttttaatatattttatcagttagattctgtttaagaattgattgttgataagcataa acatggataggacttatttaatattcattctaagaaagacttaatagggaagtcaataga taaaactttttaaaaggctttttctgaacctggggaaagtttgccatattcctgaaggtt ttattaattttttatttgttaatctgctcatgtaaaagtctgttgagaactgagggcttg tgagagaggagtgccagaactcgtgtgttgtaaagcgagcaacgtggacactgaaaggaa aagcacacatccaggttacggaagtgacctgatggggaacttgccttgtgtgttcttttt ctctccttgtcttgttttattacttgacaagcaaaggactttactgtctgtcttctcttg ttatctttttctccttgtttaaagttaaatcttgctattccctgtggagcctttcctttc tttgctcttgtcctttctgtgcattttcacctcaggggactgggctggtagcctttctgg tgcagatcctatctacatgcagacttcctgtatcatcctctaattcctgggagactatca gcctaaagtctaaagctttaggagttgggaggagcagacgtttctctcttggtgtcgcct gtgtgctaagaaagtagggacataaatttcagtctttgtcattcacttgaattttatcct ttgtatgcaaaagacaggctgtaccttcaggtgtctaaaacatatgcatactttgtgtgt gtgtgtatgtgtgcgcatatatgtgtgtatatagttgtctctcagtattagttctagaat ctcccaaggctgctagactctgcagcactgcttctaaccaactgcatcactttgaatacc ttgtctctaggttacttctaactaatactgtgtgaaggtgatggtgatgtaaacaattta aaaccctatgtttaggacaaaaaaaaaatgtgtgtattcagttgcacaaagcaacttttc tttgtgtgtgtctactcttgcatatgtgtgggtagaggccagaggttgacatctgctttg tcttacatgtttttcttattttttgagacaagtctgtcactgagtctggagctgatccat tgggataggctgactgccaggatgctctagagacccgcctgcctctggcttcctactgtg gttgggcttcatgactagtgtttaacattcctcctaggcattggaactgtctccatgctt gcatagcagactgttgtattgattgatccatctcttccagtacaacatcactttatagac tattgtttgtttgttggtttggtttttggtgttttttttttttttttttttgagacaggt cttctctatgtaacctttgctgtcctggaacttgctctgtagtccaggctgacctcaaag gctaactttttatttctatctttagtgtttcttcagttaaactgaggatgttgaatctgt ggatttggagcccatagatgttaaaggccagctatactttcatgtatatttgcttctgta ccctactattctctgcctggaggagtctttcttttcttagatcttacttaaaaagtcatc ctaggaaacgtgccctgattaagtagagtgagttattttttacttacagtgtctttagcg tttacagaatcagatgtgagtctgtttttctctagtctctttgagcataggggtcatgtc ttatttactttgtttagtgaatttccctcccccagataccttaacattactgctacatag gatgtgtatggcttgtgaacaaacatcagaagagaacacacagaaaatcttttcaataat ctctaaactggaagagattagagattattttgtcttgttatctgtctattcaggtgctta gtagtgccttctgtagaggtattctgaaaatttttattgggtcaattagaagcaagtttc atatgatatattttttttatttgcacctatgtgtatgtctctgtgaatgccacatgtgtg ttagtgcctgtgaatgccagaagagggcatccagttctgtggggatggagttatagacgg ttgtggcccacccaacatgggtattaggaactgaacttggggatctctgcaagaggtgcc agcactcttaaccagtaagcctgagagggaaaacttcatagtagatattttctgtgtgac tttccttcttcctcatttctaggaagcatgtagtactaagacagctttgtacccctggga gaatgtgccttatgctggcttttcttgatttagtaggaagattttgagacaacacaatca gtttaaacttttctttaaacttgtctgcctttctggggctggagtgatggctcagtcgtt aagagtattggctgctattctggaggacctggctcaattcccagcacccacatggcagtt cacaactgtaattccagttccaggagagccaacactcccacacaaacatacatataggca aaacactgatccacataaaacaagtaaataaataaatctatcttaaacaaacaaacaaac atctgccttttgtctaagagattagatttctaattatctttcagatattgcctgttcaga aacttttaccattgccaaatttttcatagcctgcatgctgagctgtgtaatccccgatgg gataatgacccatttaagaaactgatctaaaatgggctgaaatgatgctcttgattgatt tcttttttattcatggtccttgtctggtgttgtgtttgttgaaaaaacaagaatgttgct tattagccttacattatataagagctgttattaaataataataatactaaatgaaacttg aaggcaaaccaagtgaatatgttttttcaaatgttggccatagctgaggtattgaggctt gaagtaaggaagtcgccactgcatctctttccttttgatgatggattttagccttagact tgcccaagtaatttacaggtagttagctggaagggagaattatgtgaatctaggggatag gcatatagatttgatcatctaataaatgaataatttgatatttgtcaccacagtcatgat tagcacttagtgagtgcttcctgtgtgtcagtcactgtgctaaggactcccccacacagt gtttacagtttacttcttactttatctcatgctgtaggaagaggaaacattgagagcatt gaacacaaggctaggaatttgaactcccagaagctctgtaagctgcactacacaggagca aacattgtaggatttcactcttagaagcagattacaggctcagaggaaagcagttcccaa tcattgtaaaatttaagtctgtatttgaatgactttccatgagccttagttttcagtgtc acttttgccaggttaccaagacattttgcttcacttgcaggagtttattaaggtaaaata tatttaaaacctgaagcagggatgcagtgggtgcagttgagtggtagtgtgcttgcctag cacgtaccaggcacctagcactgccaggacgggcgtagagtgaagggacttgtcaatttt atattcaattcataattttaaaaatattaattaactaattaggacaaggtctcaggcagt atgagctgaccttgaactcctgatccttctacctctagttccccactgctgggattacag gcatgcactggtgttttcagaggaggagatgaaactttgaaatatcatctatgaattgaa aattatggttggatatatatgtgaagcatagcagaaatttgagaattaatacttgttaat ctcttgaatatgttgtttaagttagtgtggttctgtggaatatttccctaatgttacttc tttttggtcaattaaagtatgtcactttttctattctatactttgtttagcatgagtccc tactcagtatattattgacaatgcatttaagaagtacttctgatatctcccctagtatgc ctttgtatgttaactgggaggcttaaaagtttcacattttaattctttttccttacaaga gctaagcctggagattgttttttaaaaaatattttgtttatttattttagtggtggaact caaaaccaggccctgtgcatgctaggtgggcagtccaccactttttagtaaaagacttca ccgatttgagaactttaaattctgtgcttttataaaatatgtatcagaggttacatgcac gagtagagtatctagtgatttattaaagattaaaaaatatcaaaatggggtggctggaga gatggctccacagatgagcttgtatgtatggcttttcctagattcagcactcctaccccc agctcataacctatatctctctgctggggcaccagccctcatgtgtacatgcccctacac cacattgaaaaataaaataaatacgtggggaagaaggtctgaattggtcctgagattctc aattggtacttgcttccaggtcttgcaacgttgtttaatccctagaactgacatggtgga gagaactgactcccccaggattgtcctctgacctccacatgcatattgtggcatatgtac agtagcatacaatcatatatacatacccacagcaaaaaagtaacatcccctttaaaaatg cataaatggtgctgagttcagctatggaagctagttgagtgattgaggcagagagagtca gagtcttaagttcttcacgtccttggacattattgacctttctaccacaggtttctttct taaagtggctgttagtattggtattgtagaaatccattcctagaaacattgctgaatgtt taatgccagatactgcagtgtttatccacctctcacatactcctatttcatagtgcaagc taggtgtgaagatggtgtgtgtttacattttcaccttgacttcggagttgacctaggatc atgtgcaggagacatcaatggctaccctttactgcctgcactgtttgccagtttatttct tatgtatttttaatagaacccagcccagcccatgcatagaagtgggggtctcttttcggg ggcagttagttatgtattctctaagtttggcagcacatgcataattcttccttttactga ttttcactatttatacattgatttatttctttgtatgtgtgcatgcgcgtgcatgtgtgc atgtgtgttggtgcctgtgggtgtatgtaggcctgtgggcacatgcccacaatgtaggta aggaagtcagaggcagcttgtgggaggcagttcctttctttcaccaggtgagttccaggg gtcaaactcatgttgtcagccttgggaataatcaccatcttgctggccctgtttttccta ttacttagtttcagactttatatacttgtataaaaatatctatctatctatctatctgtc tattataatagaataatatataaataacaagatatttcctaaaatttcttgaattggcca tgctggatctcaagcagagttttttttttttttaaccattcactgattctcgtaggcgtg gatgagaactgaggctgcctctttcttccattctctttcgttccttttccttctctttat aacacccccccccaattctttcatttccccagttgcttaggaagacagacagatgcacac taaatttataatctagtttgtgcacacagttgatggaggaaacacagccttccaagttac atccttttttaaactgaagttttaagtatgtatcttaactgactggggagattgaattgt cacttatgctaatgtgatatattggaatttgggttagtgatcaagattggcatcattaaa gagaaacaagtcatagtttttcacatctgagaagtcacctgcttttcatggctgcataaa cagtgattacaaactcagcacctgagagtctgcctaggagtctggatctgcttccgctgg gtccttcctcagggaacacccaggctaactgaagttcatctgcctgttgcctctcctcct aatgctctgcttgtcttagaggcttactggttggcagaattagggttgtagggctgatgt ctctgttttcttgcaagccacaagtcagggatccttttgagttcctcagaggcctgtctg tgttcttgccacatggccctcccaccagtatagatctgaatcaaaggttagtgatagaaa catctgtatatgtgcactgggatggcaaatggcactgtcacatgtgcttgtttgctgctg ttccctgagtactttcagtcaactttactgtggcataatttatatgaaatgagctcatct atttaaaatgtatgatttgaagagatttgagatatgtatatgtcatgaaaacactgccac aagggaagacatttcatcattctttaaagatggttccttgtccagtctggtagtccctcc tgaaataacttcatcttctgacactcattgctgctttctctagcctagttttactttggt attttaaatagcatgatgaaaaaagaggagatggtgaaaggttattcctccatttttttg tttttgtttttgttttttttttaaatactattttatcttactttacgcctatgggtgttt gtttgtgtggaactctgtgcatcataggcatgattggtgccagaagagggtgtcacatcc cctggactgttacagtagttgtgagccaccatgtgggtgctgggagttaaaccagggtct tctggaagagtgaccagtgcttttgactgctgaaccatctctccaggtccgatacatatt actcttaacttacgctttcctcagattccttcttggcaaaataatcctttatcagataat gctttatggttttgggatgtggtaaaatgattcttgtattttctcaagatagttgcagta tgctcttgttctctgttagatctccaaagctagatagatttaaataggtagcttcctcat ctactgttccagttactgtctcttaaggcccaaaccaggtaatttcccattagaaattct agtagtagtgaatcttttcactggttactggaaacccaacccttctagagaagtcagagt atactcacctcagactttctgttgttacagttcatgtcacagtgtgaatttagaagacat gaacagtttcccttgtagtcacttactgtcaccccctccccctgctccttgtataatgca gtgttgtgcagtgcaggaaggttttaggaagccttgcttcttgcagcagaagaaaaagag agctcagaggagactcagtgagaccctctaaaagctgtcaactgtcaacgagacagtgtt gatcaatgctgtatacctgaatgcaaagtttctactaatggtattgaaacagatagtagt aattttagtgtatttttaaaatgtgtttactatatatttgtataatatatccaaatatta ttacttctgcatttaagctaagaaaacttattgaggtagtgttattttaatattaatctt tgagttcctgtgcatattttatcctttacacagtagtgtcacttccttgcttccctccct tccttctctttttcctccctctttggcatgtagcttagattggccttgagtcccctgggt aacaaaagccggtttcagacgcttcattcttcatccttttgccctgttggggccgtagac agttgcaccgtcccaggggcccagaggtgagtctggtcaggttgtaatagcaggtgctcg ctatctactggcccctccactagacagtgcttatgctccagataagcaactctctctttt atgtaacattacatgactccctaagaaaccacctttgagtaaatgtttgattatcttgta ggttagggtggtagctcagaaggtttctgtggttaattttgtgaaggtaaatatactgta atacatgtttattatacagatgatgacattattgaagaggtggtggggtctgggaagaaa cttgcttcctcagtcaagagttaactagtggtaaacttgggctgagttttcttcttttaa gtgtttcactttaaatcaaagagaaatgaattcctttttgatcttactctacatattaag acatttattgttagcatctgttttactgctagatctttaaagttttgtatttttttctca gggttgtactcactacaggtctgcttgtggtattctcacaatagtctgaatgtttcctga atagaattagaaatctgttttaatttactaaaattaaaacagtcttatagctcaaggtgg cctggaatttactatgtaggtgaagatggtcttcgactccttatccttgtgtctttaagg agtgctgccctaactgcttaagggaagctatctcctgtctcacccatacttttcaggcac agaaaaagaggtaaagtagaataaatgggaaaatggaagccaagagttctcttttacttt gtttatagaatcagaagaccatgtgagggtctgttgtgccctcccaggtgttcctgtcct gtctctcagcgtaaccttgtctgtcctggaactacagtgtatgccaggctactgtggaac tcagagatctacttgcctccctcctgagtgcagggattaaaggtgtgcaccaccattcgt agctttacctggcatagtgcgtctgtttaaatgaaggaaagaggacttgttttttcactg ctgtgacagaatactcaagaaaaccaaggaagacaagatttattttggctcatcatttaa gaatggatacaagtttgtcatggtgtagaaggcatggcagcaggaacttggggtgtctgg ttgtgccatgtaacctagagggctttgcattttattgagcagggggtcctgcatatctgg gcctgtctggctgcttcatagggtagcaggcacactgttgctgactactgccacctttgc agagctgtagaagggttgtgttaactcactgagtgtgtcctagtttccttctacctcttg cactgttaggatgatgttccacattgtaagtaatttctgcctcagaaagcagctccagca gtcactaaaggaggactcccagttgctatgagaaccttaggcaacgactatgtaacagaa tgtctcaccctaaaaaactaaaaaagaagatttattacttatttgtgtgcctgtgaatgc caggagagggtatccagtctcctggaactggaatggttgtgagccacctgatgtgagtgt tgggatttgaactcaggtcctgtggaagagcacaagggctctcaacagcggagctatctt ctcagcctcagcagttcttttgatgccacttttccttctgctacccaggaagcagtgatt gctcaagttcacctcaaactgtgtaaaacgcattacctgtttattgtctctgtgcgtgtg ttttctctgggccagagtctaactccgaaccactcacgagtcacatttatttgctgctgt attagcaacttcctattgctgtgataaaacaccacagcaactgactgaaaagtgtttgtt tgggctcagagttccagagcatagggtttccttcacagtcatggtggggcagcatggcag caggcaggcgtggctgctgcagttggagcttttataacttgggccacaagcatgagacag aaagtggcctgaggatggtgtgagttttttaagccccagaatcccgcccccagagacata acttcttttaacaaagccacaactcacttccccaaacagtaccagcaactgggaccaggt atctaaaaagctgagaatatgggggacatttcattcgaatcaccagtgtccttaattttc ttcagtctgaaacatttctaaaattcttgccttttattattactttggaaaagtactggt tgtgttgaatacctccagtttttgtttaatgttttggtgacaattgttgggtaagagtac tacagaaatgctgttgtgactcttactggatggcacacagtgtcagtatgcccattcatc tggtgagaccagcactggtaagctgatgttaggctttgtaccagttgcatgctaaccttg caattaagtgtcctatggggaaggtatcttgtgattatgttaattaccttattttcatca tgcttttagtttctaatttcaaattctggtgataatttgtatctgaatgaatattattac tttggtgtttggccagtgttattttctgtttcttttctttcttctgtaaggaagagctat ctcctctgaggtatgtactttaagtgtatcaaaatagactcatgaatattttctctatcc tgtagactcagattttattctctggatttcatctatttctttctcttcctttggtgcttt ggtcatctgaggtttggtcctcaggactctctcaatctttcatcttgtctcaatcacttt ttgaggacagattatagcagcagcatcagttgtcccaggcttatcttgtccctttttttc ttccctagccttcaatttggccatctcaccaaggagttatttgtttatttttactgtggg ttgagttttttccatttttttctccattagcatctttaaaaacgtttgtgttagaatgac tttggatggcacagacatatgaccctgctgtaggaatactagtgcatagccagtggttgt cttgtgatagactttgtctcccagcaggttcactcatttagtcatggatgagttttacac cttaacattgctcacatgttactccatggaaacaactgatatttttctttggttttgttc tgtgtttggtaataacaatttaaaaagctcttctaaaagtttccttctaatatcacatag tatagaaatgtttatagctctttatttatctttatttatttatttatttatttatgatgt tatgatagtttcagtttttcaataaagtttaaactgcttctacgttcttctagttcactt ccatgtcagagatggcttactctctagaatacatttgtctgtctgtctgtctgtctgtct gtctgtctgcttgcctgcctgcctttgtgtgtggtttgggatggtgtgagctatatttat atgatgcatgcacatgagtatgcaggtgtatatgcctgtgcacgggtatgtggaagttgg aggaagtctttgagtgcctccctttttggctctttgcggtattatgttgacccaaggtct ctccgtaagccaaaagtttggcattccagctaggctgactggctagtaagctcctgcttc ctccttccagtggtctagctgaggtcctcatgtttgcacagcaagtgttctttctgtgag ccggctcccaagtcctgcagtgcattttacatcaggcttcacagtgcctacagtagattt catttttcgtcctgctgctttggtttttaaagcaatgtttgttctcaatttatttcctat ctctgttgtaaactgcagcttgaaatttgcaacgcctggaaagttggaaacaaggaaaac atcatggagcatggtttataagctacagcttcccagacattgtgcttttagctaagcaag gcattaactagtgcccccttgtacttgggctgtgtcagtttgcttcacaaaattcatcca tacttcccattaatttatcccagttttatttctaagtattttcccatggtcagccatggc agagtcaacctcaactccgaatgggcacaaacacacagatgttcctggctgcagccagag agtagtgtacttgctcacttctccaggcgttcttgatgtgttctgaagctggaggagtac tgatgcatttcctagtgactgcttcagaccgcactgggaacaggcgtgccagctcatgct ctctgtctgttgaaagcagctcttaagtttctgcatcagtctgctgtttttcagctgttt cttgtcattttttttgcagctgatttgcttaataattctaattagcttttgtttatgggt ttatgttaaaaagtataaattttgattgttgattgtgttatggaaattaaatttcatcat tagtgtctatacttgggtagctagctcacactaaacttgacttttggatgcttcagttca gtgtgttcatatgcagtgagatatgagaaagtaaacactttttttgttgttgttgaatat tgaaacacattttgtttagtttagagttaatggtgggttaatttctacgaaattctttta ctattcatccagtgaggagagagcacaagatctttaaatgacttgtattttgttttcatt cccacagactgggtaggatcactattgtattgtattggcacatacacgctggacagctgg tgaactgtatccccaatctttcataggctgtctcagaaaactgagcacgagcattttcaa tgtgtgtcatacagggactaaagctacaagagaaatattgctttcttcttagatatttct agcatagaacacttggtttgataaacctaatttttagtttgctattttttgacatatatg aaaaattagtaaggtccatgtcattatcagtagttattacttggttattaccattttggt ttttttgacacagggtctgtgtgtagctggtgatggcatccaactcacaatcctgcttta gcttgttgagtgctggaatcacaatcctgtgtcaccatgaagggcttattttaatttttt aaaaagcagcatcttgacagtatttcttttatttggaagttctttcagaacttgtctgaa cttgtctgaagttgtgattggcagtgcccttgtcatttgactcatttaaaactgaaggaa gtaatttttcaaaatttgagtttattgatatttttacaaaacatcttcagactagccgag agtggccttgactttgggccctcttgccaccacttccctagcgcagggattgtaggcttg ctccagcatacctgcttatgcgatgctggatcaagcccaggtccctgtgtatgctcagca agcattctaccaattgagtggcatctgccagctgagccgtattgccacttgagctgcttc cttttgtatccactccttagaagagtataattttaaattgtgtataaaagataataaaat aattcagatagaaatcaaaaggcattttaatttagtaaccatctaagatttatgtaattt ttttttccattttttattaggtatttagctcatttacatttccaatgctatactaaaagt cccccatacccacccacccccactcccctacccacccactccccctttttggccctggcg ttcccctgtactggggcatacaaagtttacgtgtccaatgggcctctctttccagtgatg gccgattaggccatcttttgttacatatgcagctagagtcaagagatccggggtactggt tagttcataatgttgttccacctatagggttgcagatccctttagctccttgggttcttt ttctagctcctccattgggagccctgtgatccatccattagctgactgtgagcatccact tctgtgtagatttatgtaatttatataggcatcctcacaacctgtattgtaaaaaatttt cagtaaatacattaaactttatattgaaagttgaaaaacattcacttcaactgaaatagt tagtggatactgacaagaccagtactatttttatgtataattttgaaaaggcttatgtca taggaatattaagtaccagggaactttttaatcaagagccccgggaagagctgctcagta ttaaaattttctttgtagcatgcttcactgccacgaaggattaaaagaaatgataattca aacattgttcatgggtaaaaatctatgtaataagctggcaggatggcaggaaaggtgatt gccaccaagcctcatgacctgagtttgagtctgatgacccagccttgtccctgtcactca tctgatagagaactcacttagatgagttttcctctcacctttatacacgtgctgtggttt gtgtttgtgtgtgcatgtacacacagacagacagacagacacacacatacattttagaaa agacgataaatcaggaacaatctcatttaatagctcttttcacaatatatatgtatatgt ataaaggtctcaagtttagaacaaatatttaagttacacattaaattctattgtgggaag attgttaatagatctcaaatgtttaatattttgatttttgagtagattatcatctgactg tttctacccaccagctcttaataatgacacggagactatttatttatttatttatttatt tatttatttatgaaagcttaggccttagcttgttactttctaactagctcatataattta attaacctgtttattctaatctacatttgccatgtggctccttacctctcactaagtctg atttcctcagtgtctggctagtgactccctcacttctcttttcttcccatagctactatc tctgcctggaagttcccctttcctctcctgtctcagctataggctgtcagctctttatta aaccaattggaaggtggcggagaagaatgtttacaaaatactcaggcaggtgatgatcga ataaaaataccaatatgagaattcagtacatttgaataatacaaagacaacctttacaca atgcacagaaagattatcccaatagtagatacactttttttttttttttttaaagtttgc gtcttctgtaatgtacttttaaagctagaggtagatgtgtctggagtagttaccacacca gactttctttctcttctttgattaagtccagcagcattaagttcttcatgtctgatgacc acaggaataaggagtatttctgctggcatttatcccgctgactgttaggatttgcacaat acatggtgatgcccagagaattgctttaaagtcagaggaataagcccatgtaaaaaagga ggagacacttgagcaatcctgtaagcttttcttttgagagaattcgtccagtagtttgtt tgtgtaatgaattctgtgacaataattttaggaccactggtcctgaagagagttttgtga ttaagttgaagaaacaccatatactgtaactatagcctattaagattcatagtgcacatt aatatgctaagggttctgagccatctcttgaaaaagaagtttaccaaagcttgcttatcc tgtatttcttaagtgtcttggacaatggaattctttcattcaagcttctctgtcctgtga taaatgctgccctctagaggcataaattcaccggctcttcaatttgagcaccttaattta atacttagtgagtagtaagtggctcttaacaccaagtgcacaagagtggaatagcctagt agaaaaagatggtctgatttaattttaagaaaatgtatgctctaaattggctttattatg tttactacaatcatctttgtaatcagttctctctttggtggcacttgggatgggatctag ggctttgcatgtgctctgtggaccttctactattcactgtgctggaataacattctttta atagaagaaattgttaccgtttaatttttattacattttaattttgggaacacatacata gagttcagagaataacttccaagaatcagttcttacctcctcctggggatcaaattcagg taatcaggcttttcatcaagcaccttgacctgctgaactgtcttgtggctcaaatatagg ggattgttgacacataagtcttcatgaacctactatgcagttcagattggccttaaactc ctagtgaacctcctgcctcatcccaccacacccatctttgtctactttattgaagacctg atacaatcttgcttaagagagtgagcagttgttcccacagtccttatgtttccagacctc taactggagccaagattatgtcctacaacaccctcatctacttagattatgtcctacaac accctcgtctacttagattatgtcctacaacaccctcatctacttagattatgtcctaca acaccctcatctacttagattatgtcctacaacccctcatctacttagattatgtcctac aacccctcatctacttagattatgtcctacaacccctcatctacttagattatgtcctac aacaccctcatctacttagattatatcctacaacaccctcatctacttagattatgtcct acaacccctcatctacttagattatgtcctacaacaccctcgtctacttagattatatcc tacaaccccctcatctacttagattatgtcctacaacccctcatctacttagattatatc ctacaaccccctcatctacttagattatgtcctacaacccctcatctacttagattatgt cctacaaccccctcatctacttagattatatcctacaaccccctcatctacttagattat gtcctacaaccctctcatctacttagattatatcctacaaccccctcatctacttagatt atgtcctacaaccctctcatctacttagattatatcctacaacaccctcatctacttaga ttatgtcctacaacccctcatctacttagattatgtcctacaaccccctcatctacttag attatgtcctacaaccccctcatctacttagattatgtcctacaacccctcatctactta gattatgtcctacaacccctcatctacttagattatgtcctacaacccctcatctactta gattatgtcctacaacccccttatctacttagattatgtcctacaaccccctcatctact tagattatgtcctacaaccccctcatctacttagattatgtcctacaaccctctcatcta cttagattatatcctacaaccccctcatctacttagattatgtcctacaacccccttatc tacttagattatgtcctacaacccctcatctacttagattatgtcctacaaccccctcat ctacttagattatgtcctacaaccccctcatctacttagattgggaagaagaggattgtg tagtgtgttttaagacttgcaaaaactttatacgtatgtatagaacttgtttcaaaacat gggaggggaggaaaaaaagaggggtgaaaccacgtacgaatagacaacatgcttttgtat ttttgctgaatgagttaatgtgatttacttacagagattctagtttcagtgttttagttc aaatagttctaattatttgcccttttgattttggtattgctgtgcactaactgaaactca tggtacagtttaatggaagtgaaaaagttaaaacaaaacaggattgatgaagtccattta tcatgttgaccattcagctatcttcctgactctccagaaaattccacttatgatcttgaa catactgcatttacctaggtgggaataaaaacctttgtggcactgtgactgctgctgtgg gtgtcttttccctctttggaggaagggctgataggattctagatgctctcagttccgtaa gaaagataggcatcatttcataaggaaacatgctaagtgtgatggtcaaagaaacactgc ataaattctccttggctatgactgtaaaatggtagaattcctagatctcaaataggaggg cagcccattagaatctgttaagttctgtcccatgccccaccctcctaatgctggggattg aacctaggtctttgagcacgatagacaagtagccctgctacttttttagattgtatttca aggtagtatctaagttacacagggtttccttgagtttgtgacccttttcttggcctctgg gtatctgctagagggatgactaagttccttcttatatgcttatgtgctaatacattttcc ctcattcttttcattcctactccccttttgtaatttagtctgtaggttatagaaaagcca ggcaacggtcatcacctagaatttctgctattggagctgctgtagcagctggtgggactg gttttttaacgtttttgtgaggagcagaagatttatacaagtcttagagtttgtcatctt gagaattttgtttagagtttattgttaaaaataattgtgtgtgttgggtcatgtttttga ttgtttatttcagattgcttattggtattgggatactatcatactgggctataattcaca gaatcctttgcaataagatacaggcatgtggtctaggcactgtgcattacatggatgcat aagatacaaatcccaaagagaagtgcaagctaacagctcttgattggaatggctgcttca tgtagtttgaggcttgagaccctgagcttgtggggcagcagtgcggaggggccagtgacc ttgtcctgctccctgggcttttgtggtactagagtgctgttatggggatcatttgatgac tttgctgtttttctgctgtacagcctgtgagtggatgctttctctgagagttacttacat gttttaacaagttcttttttgcaacttagatcctggttttatagatttttgtaaatattt tatttttctgagacttaaaaattgccaagcacatctttcacagttttccttccccagagg tttacattttgacaccatttttcttgcttggtgtgtgttttgcacctttggattgttttg atgtttgtccttcacctcctcagcttcagttccactgtagttaggaaacctttcctccag ctggcactttttttccctgaatgccagccgaagttgtctaggtgtgaagtgttgcttttt ctttctgtatgcatcttagtggcacactagtgataagctcccagtgagctgctaacaggc ttttctgtctcttgcattaccaggcagaatgcactgtttagattcaaggtcacctgaaaa atcacagtgaagccaggccgagtgagctgagataacaagctagctacatttgattcacct tagcaccctgtccaggatgtccaatttccttcttccttacagagactcagatctgctctc attaggaggtcatggcctcgccatctgaattacttactgtctttgtaatggtttgtgatt tttgccttgacaaaaacctgtttgtctccaagttcctttcattgtaacttttggatttgg tattcatcctcagcaagcttactattttaaataccattataactctataaataatgaagt aaatcagacacactgtagaaatccagaaggtactaaacatacctcttgtgcccaagtcag ggttaaggagccattaacaacctttgtcctcctttgcatccctcatctgatgctctgaac tgatgatcctcctccagtctcttcctgacctgtaggggctcttcttcctttcaagttctt tccttctcagctcgtcttcctttgttactaggtacccggtggcctaaggtcctttggcca ttccactcttgtttttctatgtctgagatgttcataaaatgacctgaacagttttacatt tcattgctttatagatgcattccaattgacataaaaaacttagttcatttcttactgcct ctgttacctttgcatgttgagtaagagtcagttttgctgagagataactaggcttttttg tctttggcaagtggtgcgtaatgctcgcacatggtatcgtaatcaagtgtgtgagttgat ttgcgataggcaccagaaagtaggggctcagaggatctaggttcaggtattgctttgggg tccaaaagttacagcttgatacctcccaccctgtttcccttaatttttatctagtggcct ccagttggcctgtgtgagtttgatgtaaaatttatttattactttaatttctagtgaagt tgagcatctttcatcactctgatatcctgttctttgaatcttttcttcgtatgctctaca tttactttttgtattttccctttatattgatttctggcactttttatagtttgtgcatta gtgttttgagaactaagcattacaattacttttctaatctttgttacactcagttctgtt agtgttttagtttgttgcttgtatctatagttgatgttgagtgtcagcctgatagattga gccaagcctaggacctcagtaacaagcacttctgggtgtttctttccagagtggattaac tgagggcagagctgccctgactgtggactgtaccattcagagactgagaggcagataaag tgaatgtgggaacgaaaatcaggtgtacacagctgtaatcccagtactccggaggtcagg agttaaaggtgcttttggcctacacagaagagccctgttttcaaattttggggattgaga atgtactccagtggtagagtgttagcatagcatgtgcagccttgggctagatctctagag ttgcacttcacccaactccagcaagaagggggcggaaacctgagggtggaggtactgtgg gaagcctgttggcatagacagacatcctgtctttctgtttcctgaccactgatatgacac catgataagagtttctctcctgtgtcacgtcctcctgccccaagtcagagggaatccctc tttaaaatgatttctgccatgtatttttagtggtggaaaaagcctgttaagaccgtgagt tttatttgtgttgtttctattctctgggctgatttggtttgtcttccttcatgtcagaat cacactgttaaatgagaacagttctaatgtgtctttgaaatttaataaagtgaaccttct ctgaaactttcaggtcagcttatcaagtttcaaacatccttccaaaataatctaatatca agcaatttctaacaactcttggaattatgattggaacctcactgaatttacagttttcag tgcggtaacagtcatctgttcctttatgtacttttaaatgtcttctggtacagttgatgg ctttcatagacataatctttagatttactcttgttactgtagctctggctggcccagaac ttgctgtatagactaggctggcctcaagctcagacatccacttgcctttgcctccaaagt gctggaattataggtgttgccaccatgccccatttctagttccacctccccatttttgtc ccccccaaaacttttaattcaaaaaataggttctttcgggtatcctctgttgataagcct ataattagcaaatgctgcattaatgctgcccaagtctgttccttttaatttttcttgtac taacaacattgaagcccttccttgttcttggtcatttttatgctagtgtgtggaagagag gtagatttgatttttaaggaccaaaaagtcttaactatagtccttataaaggttaaatat ctagttccaacaagcaacctcttatagggtagacactttgttcctttgtagtcttgttag ttgtactgacatgtgaagatcactttcatgtcagctgaaagattaatttataagaatggc tttgatatatatatatgtgtgtatatatatatatatatatatatatatatatatatatat atatatatacacgcacacacacacacacacatatcccttcttaatggggcttctgaggga ggctattcagaactggaaatgtactcagtatttaatatatttctgtaactgaaactactg taactgaaactactctgtttggatgcttacggtaacctgaatccttcttgatgtgtgagt tgtcttatactttgttataacatggcttaagagaataacttgataaaccactttatactt catgctgtttccttcatcagtagaagtatcatcataccttaccatctaaatggctactag aattaagtaattcccagaaggcattcagaagcgtctgatccctgggattggccgtctatg ggctgccacctactgcctttctggaaatttctttaaattctagaaagatactagtacaac tgcatttccagccctatagtaactcttcttggcttagtacttgcatggatgactagctag tgttctgggttttactttctgtgatttttttttttcccctcctctgcagcctattctggt tggcctggaaattatgttaatgattgttactcctaggggctggagagatggctcagaggt taagagttcaatttccagcaaccacatggtggctcacaaccatctgtaatgggatctgat gcctttttctggagtctctgaagacagctacagtgtacttgtcatatatataaaaaataa gatttaagattttatttatttaaaaaaaaagattgttacacctagttaatcactgtgtct ttcaaggaagccaggttagttgctgccactgtagctactgtactttctgtgcgttagctt gctctgtttccctttcctattttaacttgttttctttacttttatactacttaaatgctg cagtagaaagccaccccaatgggtacacatatacacatgtgtatatatgtacatacatgt gtgtgtgtatgtatgtatgcatctaaactttagattataaatgtgtacttgggtatattt ttgtaagtttaagattatagaaactgctataaattcatgggcatcaacaagtagatgttg gcatagcttggctgcctgatgcagtgcttcttctgttgactgcttcccttggccccagta cctaccattcagtcatttgggctctgtaggctcccacaatgtctctttctacagaaggtt ttgttctcactttctagggaagacaggactccgtggtgttccttagtgtcttccttctta ctgggataggctcccttcctctccacagccgggcatgctctgctgctctgcgtcattctt cactgttaagccaaagttaaagttattttcacttagctttttccttcaatttagttatta aaaaaattaaatttgcttgattcttggttctagactattctctcctgatttttttttttc tctttccaggcattcgcaatatcatgactccatgcagctatcttagaagtagatcagaca aaacaagtctaaatctgagttagcagtcctcactcacataatttcacctttagcctactg tctcagcctattctcatggggcttcactgtctgtcttgtctgtctctctctctttcttat aactattctggccttatgctctacatgtactatatttttaatgtagaaaaagatactcat gtcagtcattagggaggtgaaagtttagcctgcaagatagccatcaaaaaatagccagaa gggctaaaattccaaggactcacaacagagatttccttgggaagataccccaggaccctt ttagatgacacctctggcagcctcttacaaattgaaacatgtactactactggacattgt cctcctatgtattccaaagagaaatgtaaacactcattcataggaagctctttgaaagga aggcgtactagatttattcagaatatctgactactggaaacaacccaaatatctttcagt agctaatggctggagtttcctgcagtggaatgctactcattaataaaaaggaactcccag tgtaaaaagagacatagatgaatagcacagacacactcagcagaagaacctggatacaga attctataatttctcttatattaagttttagataagacagagctactctacgataaaaag taaaaattatgaccactgactgcctttgagtttaactatgctagtggtggatagttgact ggatatgtttgctaaagtgatacaaatgttcagtgtctgactggtatatgtgtgcgcgtg catgcgttacagggtatatttatttgttggacagctaagatatgtgtgtattttctggta tggatgatttatctagtgattataaaaatagttgaatgactgggagtgggcggcaaagat gcttgaaatttccacttaagatttttgaaggtcaaatgttataaagatataaacaacaaa tacccgtgcatgcatttatattcttcaataaaaataaccttattcatatgataaattcct ttctcttaggcttctatactgactccttactcttcagtccctttttgctgtttttttttt ttttttttttttttttgaggcagagttttactgtgtagcccaggctggccttgaagttga agtccttctgctttaagcgctagaatgcaggtatgttccaacctgcccaccctgttctct gctttaaacatctgtatgtttgttgaaaggtcattgccatgggggaaagctgctcttgat ttagaacattcactagggtgtgccctttcatagtttatcacagttatggttggaattaaa gtgacccttgcaggttcatgttttgaaatcctgttcccaagctggcgctgtatggcaaat gggcctgatcggtggaggttggtcactaggagtggtcttgaaggttacagcccggactct gcttctgggactttgtacttccttgtctgttgtaatgtgaggaatctctggcacaggctt ctgccgacaggaactcctctccacccttcctgcagctgtgcatggggatctccacccttc ctgcagcagtgcatgcagaccatttaaattgtgcgctcagtaggtccttccttgtgtagt ttctgtcaggtgttttgtcagggcgagggacgagacaagtggttaatacaattacatgct ctctccagaagacttgagtttgcagaggcagagctgttctagtattgctactactcaggc tttttacatcctagtgaacagttcttgagaaaagcaagtaaggaacacatctctctgtat gtgtgtgtgtaagtatatgtgtacatgtgtgtgcatgtatgtatgtatatgactacactt tatttcagctcctgattttagagacttgtgttgattatgatgaggagggtatagcacagc aaagcatgaacatcacaacagccttggaaggacatagaaggagatccagtggggcaaggg caagtgatagccccaggttcacactccagtgattgatttattccagtagacccgcctcct actcatcaccacctctcagcaatacagtcacgagatgaatccatcaggagattcatacat tgattaggtcagagccctcagaatccagacacttctgaaaatctgtcagcagacaaccaa gcccctaatactttagactgggatgggaggggagatgaggcattacatactcaaactaca gtgcattttcatgctggggtatgtatatataactctcttcccccacaaaccctatcttgc ctatagctctcatttgcttttgtcacttaacaaaacaccacagacatctgctttatgatt accttcattcaaaacacagttcaggctggagttggctcaatggttccgagcgagcgcttg ctgctcttccagtagacatgactttggttcctagcactcacatcaggcagctcactgcag tctgtaactgcagttccaagggatacaatgttctcttctagcctttgtgggcacctgcat agaactgcatgtaacatacacacttgcacataattagaaaaacaaagcagttctgtcgct tgattttaccagatattcactttttatctatgaaatgtgaatactaacagtttctgtctt ttaggacttggagagcataatattattactatttcttaatgaggcatgcaaagttcccaa ttttaagtgttcagcatgtattgaagaaatgttggcagaactagccattttgttccttta aatttttttctcatatactacattttgattgtatcctttcctttgagatttttcccacct cacttcatgctctcactttctctctttctctctctcagaaagtaaaagcaaatggtcaaa taaaacccagtaagataaaaaaagtaacaaagcaaagaaaaagcataggattgccccaaa tccaaaccaaacccctaaaccagcctgtaaagtctgttttgtgttggccaactactcctg ggcttggttatatatcaattgtaactatcttcttggttaagggtggggtcacttcccctt ctctttgctccagtgttgtctgggtccaacttcttgtgagcattgtgcatgctgttaagt ctctgagctcatatgttttatcagccttgttattttggaagatgctgtttccttggagtc acccactgcctctggctcttatgatctttccatattcttttacacattgatccttaagcc tgatttccaagccgtctgtgttgactgtgggttcctggtagagagctggtttagggatca gttgggaagctggtccctaaaaggggttactgaatgggataacaaaaagaacgaggattt taaacatttttaatgtagacagtcaggtggcagttatattttgcaacaatcacttttatg taattatttttataatcattcttcccagcagaatgctttaccagcagaataattcctcac accttccccacagccatttgtgacttctgttgtgcagtctctgtgaacttgagggttcca ggtagcacatatacatgcatttataccgtggttgtctctgtatgactggcttatagccgt gtggcataatgctttcaaggttaatccatgtcttaccatgtgttgtcacactgatttaca acttccctccttgtgattggagaagacactttcccagtggatgtgaaaacatgtccatag agttttcccaagaaaattgataataaatttattaaaaatagctaacgagtagacatgtac cagtaccatggacattcatagtaagattataggaaggtaaccatgatcaaggttatatgt tctgagtgctgggctggcaagatggtttagcaggtgagaatacctgctggcaagcctggt gacgtgactcctgaaacccataaagtgttagaacagaatcagctcctgagagttgttctc tgacctgcacatgcttgccattgtgtgtcctctaacaataaaatataataaaataaaaca ctcagtgttttctattgaatttagaagtagcagctttgtatcttacagttctcaggaact tgaaggattgaagatacctgatattgtagtcatttcatgtattaaacaagtagtcatatt gatcatgaattgcatttaaaaatccagccttcatgttcttcacgtaccagtggtgcctca tttccccaggtctttatgccttctgaaggcattggaaatagagcctttagggctggagag atggcccactagttacagagcgtacgtttgctcttgcagagggtcggagttctactgtag cacctacattgaggggctcacaacttcttgtaactccagctctaggtgcatttcataaca taactctggcctctcagggtgcctgcacttgtgtatgttgcatttgtgcacacacaacca catacataaatacacacaaaccttttaaaatcataaaagaaaaggagaggttttggtagt tcagatagttgagaggtcttttgggggtgggggtgctggggatggttcacagcagtgacc tttctgaataacaaaccatagtgcttcttacaaccatgtagttaattgcccattgtctga aaccacgtccagattctagagtaactagacacttcttcatgaaccaacagctattttcca atataataattcctaaagctagttccctatatggatggttacacttagtcttactaatgt attaaaagtctggttgtggcatttgattttatatattttatatttactttgtatatatgc tgttcacataacttacagtgtcctttctttagtctcttctatttgttcaagcttaattat acctgttaaatcatatttagtagttaacattatcatattgtaatttactttagttttcct aggtgtacatcttattctccagaataaattgcttgaggtcgaagaggagttagtttggca gtattttctgtgctgtgctagctgaagctgacagtgctgattggaagtgatcagttatgc ttttgattttgtgttgtgttgtttctgagatatttagtaggtagcatggattttatactt tggtcttatgggtaaccacaacagaataaagtatatattagttaaattctcaacatctct tataattggatgtattgtagagagaagatttgagtttagtgaggttttaaagtaaatgcc ttaaagaaaacctcaagttcatatgataaaacagagaaacatttcaaaattgaagtccct gaagcttctgatgtaagttgaacaaatgttgcttccgcagccacagcaatagaggagtag ctttattggctgaggcacattagtagctttgtcgatgagaagagctaagggtccttattt caattcatggttatgctagagtagtgaagtgagagctgttgacctgctcttaagcagaga ctagaaagtttttacaaatagattaaagtacatagagaggtgtgaggagctaggcatgcc tttaacattaggaatttcatccttttttgtccagtcttaatacagttgaccctacaacac ttactaattgattctgaaaacaacagctttaatgaggtggtttaatagagttaaggtaat agttgagttgttggtacatctctgagtgctattggatacaaagagtaatatgagatacag gatttattaaactggagacaatgtaatgccactaggatggcacatctccctatttgtttt atgagtccgtgggcatgactatgtggaggtcaggggacaatacttggagaagctggctct gtgtttccatcatgcagacccaggggattgaactcaggttgtcaggcttggcagcaagca tcttcatctacactgagccgcccttttggtccttgaactgtaacttacctgttaggtttt ttttttttttttggttttctttctttttttttttttttttttaaattttactttcataat tttcaattgtgcgtattatggatgtttttctacattgtcaggttgttattttttcctcat ttctttttattctctacttttcatatttttcagttctctcatgtttttattcttaactca tgatatttttcttaattttactttcctggagaaggctatcctagtagaggttataattgc catggtgaagtactataactaagaacaatttggagaggaaagggtttatttggcttttgt gttctgaatcaaagtccactgatggaagcccaggcaggaactcaaataggatagaaactg tggagacaggagctgatgtagaggctttagagggatgttctttgctggcttgcttcccct ggcttgctcagtctgctttcttatagaacttaggaccaccagcctagagctggtcccacc cactgtgggatgggccctcctctattaatcagaaagccctgtcagtgcttgccaacagca agccatcttctggaggcattttctctgttgaggctccctcctcttagatgattacagctg tgtcaagttggcctaaaactgacaggacgagaatcagctcctaattcgtttcttgctttg tcttacccattttttatttgtcttttgtatttttagttaaaggtggaaaaaaaaaaactt ttaaatgtttacttcagtcattccagttgatcttactttattgtgttttctgtggctttt gggttttaaacttagctgaagtttgacttggattatcttgagtacttctgcttctgttcc ttagagtcctacaagtgggagctgggaagaaggagggctcagtggttaagagcacatact gcttttctggagaccagagtgaggtttccaggatccatatcaggctcacaacagcctgga actccattcccagaagacccaacactcttttggcttccaaggataacccctgcccacaat gtgcatgtatagataagtaaaaaacccaccaatttttgagtaccatttaaatgtgatata ctaaaactttttaaaaatagaaaatagaattgttttaagttttttttttaatttagaaga aattttaattaaaaaagtacatatgtttatggagttccatgagatgttatgatacatata tgcactgtgtgatgtttatggtaaggttaatgcatcaacctctataaacagttaccattt ctttgtagtgaaaaactcaaaatctagcttttttttttttttttttaataggttataatg ttgttctgtgtaatgattaggctactgtgcagagcactggaacttctagctctgagctag agcctgggaaagtgctggcttaggtatcagtgtttgctggttaccatgcaggaagaatct catgaacttactgttgaacagtgtgtagctgcttaggtaactgtgattttctgctttagt ggacattccatgcttcttccatccttacagtctttctgatctctctgtgttctcagaaag cataaaactgggtacttactccttaacaaacccataatctgcttctgtgggctctttaga taatgcatgatctgttttttctcttgcctttggtcattgtagactcttaacttcaaactt caaagtagatacctctccccctcccctcccctccccatcctcccctcccctccccatcct cccttccccatcctcccctcccttccccatcctcccctccccatccttcctcccctcccc tcccctcccctcccctcccctcccctcccctcccctcccctcccctgcctgccttccttc ttccttcctgaaggatagatctctttggtgtaagagttcagtcaggatgacaaatctctt attatgaaggagtttcccctttattgttataagatacctctccaattaacatttacatta aaggaggctgatatttctcttaataattgagagacaactttaaagcagtatggttcatgc ttggagggggaatgcagtctgacttaggaattgatgcagtggaagtgttcctgtggcttt gctctaagtgttggcatctgtattgtgtggctgtcttgtcttctcacagaaccctcataa ctcctggctgattgagctttctctacaggtcaaattcttatattcttgtaacattttggt gagtctacctctaatgaatgctggccttaggagaaacttaagtttctcagcatctgggga gtgctgagagagcttatctttaaggactgggttttcaaacaagttggtttgtaaatggtt aaaggtgcttgctgccaagtctgccaatctgagttcaatctatatgacctacatggtgga ctaagagaatatacactgggaattgttctctgatctccatgtgtatctgtggaacttgtg tgcatttgtatacatgtacgtgcacacattaaacaaaatgttaaaagaaagcaaaacgtg tcacagtccatggcaatctgctgttgtgaattacaaagtgtaggtggctttttttctcaa atgtgttaaaaacttcaaattgaatcaggatgctaatttagtttagagagaaatagaaat tgtttacattaagagacaaatggaaaagtaattaaaaagaaatgagactgcttatatttt aaataaagaagatactattgtcaatattatgtagaattttagggatatcagaatcttctt ttgtaaagcattcttcagcaggtccaaaagactttcttaaaaaagacattggttagaaaa aattcagtttgtaaaacaataggactgggtagttttctttaaaggttttacaggtccctg gcacgaccaccatctgttcactgctaattctttatatgtgtgctggattatatatttgta attttgtttgtgtgcatatgtgcatgccacagtgcatgtttgtgtggagctcagaagaca atgttcaggagttggttctaactttctactgtggattctggggatcaaataagccaggct agtaaagttctcacttgctaagctatcttaatggcccatatgtttgtggattttacctga acctgttctaaggtagacctgggggaagggtgtgggggtagggaggagataagatatggg gcataaataattaattaaaaaatgtattagcaaaatttggtgttaagtggtagagctgta gagtgcttgccttgcatacatggcatcctaggttcactttgtccccacaagaaaccattg acaaatagagctagggaggtcgctcagtggatcagagtgccttatagcacaaacataacc agtagtgtctgtgtacccttgtgaccctagtgcttggggcaaaggtcagttggaggcaga agaatggctggggcttgctggcctattagtttagccaaaaaagtagcaagctctaggttc agtgagggaccctgtcttaaggggctaagggagagttgacagagaggatacctaccaggc tgccagattttcagatcagagttttctatgtcactagtctgatatatcgtctaaacttgt tttattgaaaacaaatttgtcaagtctgttttaggaaagtaaacttgaaaaatggaacta tagttgaacgttctattttttatttgttgctatattttcacaaagtgaaacccagcctag atagatggagatccattcttcaggagactcctgtgcctttcactagatagtagctccttc ctaagagaggtaatcacctgtgactttatcagagatatactttgcttagcctcatttttt tttttttttttaaatagccagatcctaagtgtgtgcttgtttaggttcttatttcatatt cttaatttagtttctgttatatgactatactgcagtttatggcccctgctgctgttgata cttggattctgtacagtgtggggttcataaggataattttgctatgacagttcatcttcc tatctttttgtatgctctcactcactgttggtagtagatgcctaggagaggtgttatttg gtcagtaggagtgtgtatctctgtaccattttatgtttctttttccagaggataaaaatt acagttgcagcacatgcttgcttgcaattaatattatgtagttttaaaataaacaaaata ttttggtatgcagtaatatctcactgtgataacaatctgtatcttcctgattaggttgaa tatgatctttagaatttttattagattcttaggctatcttaaaaattagattgccatttt attttaaaaaccattccacttttgattatgtatatacatatatgtgtctgtgcgtgggtt gtgcatgtgagtgcaggtgctcatagagtgagtacaggaggatatggaactcatagctag agttataagggggggggcactgagctaccgtgggtgctgggaactgaactgaggtgctct gcaaaagcaggaagtgccctgaccacagagctgtctctctagcatcagttgtcattttct ttctttcttttttttttttttttaaagatttatttattactatatgtaaatacactgtag ctgtcttcaggcacaccagaagagggagtcagatcttgttacggatggttgtgagccacc atgtggttgctgggacttgaactctggaccttcggaagagccgtcgggtgctcttactca ctgagccatctcaccagcccagttgtcattttcttaatgatttgaaattgttttcagcat gttttggatatgaatcatggttgtacatgtgccttgccagagtactgtatccttagcctg cattttcagtttttcataatgaatatggaattataccaatctagtttttttccacgtgtg ccagttaggaaatctttgcccaatttgaaaaagttataaccttttctggtcatcttctaa aaactttatggtcctaaattttctgtttaagcttctaatagccccctttctttctatatt tcccacggtctggggttagatgctatcaatctaattgctcttgattttagcacaagtaga gagaggaaatctattgatatgacttaatatcatttacatttatttaatttggtgtgcatg tatgtatgtgctgggtgcatacatgtggcagtcagaggacaacaggataggttccttcat cctcgtgggctctagagatgaactgaggtcatcagacttggcttctttacctcaggaccc atcttgccagctcaagaaacattttgctgccaggcatggtgttgcacgcctttaatccca gcacttggtaggcagaggcaggcggatttctgagttcgaggccagcctggtctacaaagt gagttccaggacagccagggctacagagaaaccctgactcgaaaaaaccaaaaaccaaaa caaaaccaaaacaaaaaaaccaaaacaaacaaaaaaagaaacattttgcttttgtataag ttttagatggtagcagttgtgttgttaattaaatctggtgagtctgtaaaaggaatttta aaagtttttccactttgattaagaggcagccagttatactttatattatagtacatcctt ttgagtctagtaatggtggtgatagaagacgtgtgttttcttttgaggtagtgtctctag cccaggctggccttgagcttcctattgaactcctgattttcctgtgtgtacttctaggaa cctgtacccagcttataatggtagtgagaacacgaactgtgcatcttggtgtggctgctg tgtgtaagcaggtgacttagtcgctgttaggcatgaagtacttaacaacaaaaagataac tatatgctttgtaaaggcccaagtgaagaacctgacaaatagtgagtttataactcttat tcctgaatttttgaacctaattaaagttgaaacctgactgagaagatttgtactctagtt gttcttattaagatgacttaaatctaatttttataagtagtaaaatcaacttgccagttt atacatatctttttgctatggataatatattttagtaaagccgggcgtggtggcgcacac ctttaatcccagcacttgggaggcagaggcaggcggatttctgagttcgaggccagcctg gtctacaaagtgagttccaggacagccagggctacacagagaaaccctgtctcgaaaaaa acaaacaaacaaaaagcccccccccccagggtttaaactactaatatatatatatattat atatatattaaactttgcatgtgtttttgtagtttattatctcttccatgcattatgtat acaagttatttttaagatcataaaaactttaggcagactatgtttaactctgacattgaa taattatttttattttctattattcaggaaatctcagctatcaaggagacttttaaaagg ctctctaaatatttggtaagtgcttgatttctgtgtacttttgcatgtttcttatattta tgtatatgctacatgaacccctcttgacagcagtaagttaaattacaataagaattatat ttgttttcatttttacctaggtacgagaatttcttctctttatatcttttgtgatccctc tacacattgtgtctagacaatcaggttagtaagtaggaatagtaatttatgaattaaata agactttttctacctgagattttataataattgtctattagttgccaaaacaaacagatg atgatgatgttgtttactgtttgttgctgactgttccttaatgtattaagtcttgtttat tacaatgtatgagcataagcaccgaccggtcctgtgggttggtgtctatgctgtttccca tctgaattaccttaatttctctgttctaatattaaaacgaaaacttctgtttgaagttgc ctctaattaagaatttggtaagttaatcatttcactagggtaagctatcccctcctacta gccccttgtgtacatagtatgtgtatgcggtgtatgtatgtgtgcacatgtgagtacagg tatgtatgtaggcatgcatgctgaaaccagaggaggatgtcaggtgtcttgctttgtcac cttcttccttcttctcttgagacagagtctcagtgaaactcaggttcaccatcttttggc cgagctagctggccatcaagccctaacagctttttgtgtccctcaacccagtggtgacgt tccagggccacttggccatacctaccttttatgtgcgtgctgggaatcaaaacccaggtt ctcatgcttgcagagcaagcaccgttagtcagaagccatttccccagcccatctcttgtc cctgcctcttttaggctggttcctgcctgtccatccatgcatccaccatacatgtatgat gcatatcctgcacataggggaaagcaaagcaggtgatagtttgtcttccctgagtttagc ataatcattagttaacatgatgatttcatttctatctatcttcttaatgtatttttattc ttctttaatagctgaggggaaaagaccccctcatagtttatgtgtgcatgtttctccatc cattcattcatctgttgcatggtggctgtgaacagtgctgcagcaagcactcatgtgcac agcactgtgctgtgctggctgcttcttttagctatgtgtctaggagtggaataactggaa cacaaggagttctagttgtaggtttttttgaggatcctctgtactgatttccatagtact tataccagcttatatgtatactaacagtgttgaaagcattcctttttactagtgaaaaaa attcaaaaggattaagtgttttataaaatgggataagtgctgagatatccaataatcaag taccgtggtatagtttatggttgtttttaatataaccacacaaaaatgttttgttttgtt tttcattcgctaaaggagtgtctctagatatatgaagtgggaaaaacatctgtatttgaa acagtcagttttcaaccaaaagatatttctatagcaattatagcacatgcttacatgagc agcagttacagtattctgtttagcatacacctgcaatttgtagcatattcaggaacaact aatagagcaaagatgcctgtgaagatttccactctgtgtgccttgtggtggctcatctct ggagtatatcacacagaaggggctagtttggccctctcctaaacatgaaccagctgtcat cattgtaggcttttggagcatatctcatcctaaatgtatttgtgacagttttattgtctt acagtttacacaccagaaaacgtcacacttgtgtactttccaggattctgagtctgtcag ctatgcactcctcacagccactcaggcgtagggggctttccttactgtctaacctcatct ccctctcttggcaacaagtaatgggctttctgctcgagagtttgcctgttttggttattt ataaaaatgcaccacttagtatttgttcttttgtgcttcattcacttagcacagtatttt tagcatcatatgatatgtgtcatatcagtacttaattcatttttatgacttggtattcct ttgtatcccacatgttacttatcctttcatcagttgatagagctgcttttttggttgtta gtaatgtgtattgtgaactagtgtggatatatattttcacttcttttgagcatgtgtgaa tggggaggggttgagaattaatcctttgtgtttcttttcaagtagtggtcaaaatagcaa tacactggggtcctagtctccacatccgcatgggcagccatgtgtgtattttagtcttcc tggttggtgtgaaaggacacttcattgtgggtgcttactggccgtttgttgtctctgaag aaagagccattcaagtattttgctcattttccagagcaaatacttttaattttgaattgt gatcattttttttacgtgttatgagtagtagatgtttattcagtatgagagttccaagta ttttcttcttttctatggattatccttttagtttgaaaaaaaaagtcattggaagaaatg acccattttatcagatgggtttctctgtagccctggctgtcctggaactcactctgtaga ccaggctggcctcgacctcagagatctaactgctctgcctcctgaatgctgggtttaaag gtcgtgacaccattgcctggcagaatgaagtgaaaggttttaatcttgatagagctcagc tttcattttgctttgcgtgtggcacaaagttacacgttcattttaaaataagtcattatt gatagtttaggtctgtaataggcttcaccctaagtccataaagaacactggcatttcttg tatctgacggccagagcacaaagggaaggatttctactgtatttgtctttcagttcccca tgcatttgtttcttatgtttgactctgtgattactcaatacccgaccctacctgcccttg ggggttggaggttaggtagtaaagagtcaacaacacaggctccaggagcaggtgagaaac accgcagcaagctcttctgaaaactgctgcaagctcctttaataccctctacacacacat gcccattggtcccaagaacagcagttcctgactggctggcactgtctgcatcaatccttg ctctctcaggcagtccgcacttaggtcctcctgcaggatatgcttttgcggctccttgcc ccctcccggcatttacataaaggtgaccccgctggtcctgctacagttcctatatgagat tggtttatttatattagtctgtgttgtatatgatagctccccctccacctgagatgagac agggtcttgtgcatctctggcagatcctggagatctctgcttcctaagtactggggtttt tgaagttttctttgcatgcctttggtttctgttctggaaagaaaatagattcataggaga cttatctttgttactttttggctgagaatgttcttctgatttggcatgtggccagaagct ttctgagagcctcacagaacacaaagctgatagaagaagtgctgggaagaagctctgagg caagatggcatcagaggatgtgagccatgtatctgagtacagtctctgcccagttctttc actgactaccataaactactcaggtgccagggtatcaggctgataaataggaaggcgtgg ctcaaaggtgaaggagctgagcacaggcagtagctacatgctgcaaaggacagtttggtg ttcccattcacaacactaaaaccacaagttcctctgaaagattatagcaaagtccagata gactcttacattgtttacataaggaattctgttttcaaagggctcaagaagaatacttag gagtgaatagataaagcatagaggcactttaaagatgcaggagagagttttagggctgtc tggaaggagagttaattaagtaacaaaacaagtgtttagcagtgaggagagtcaattagc ttagagtaataaaagccatcaactggaaggatgggaggatgaagagtccaagtcagaggc ctgcgagaggattgctagcctgtgatggcacaggaccataaataatggctgcagactcaa ttcagggggaagtgtcagcctacaaaagcaggaagttcaggcagaatcaacataaaagag tccacatttagctaagtaagtggtctagctgcttagttgcagagaaaatcttgtaagcac taatagagaaagaagttatcatgttggctagttgaggaactagacattcagtgagctgaa agaaaacctaaaagatgcgaatttgttaccgtaatttgaaagtaaaataaagacatgttc agataaacactggggttttctatcatcccaccacacttatgaacagttctataggaaatt gattggtttgaagggaaatagccccagaagtcatatcgaagctagtaaatacataatgta ctatatttttctaattataaaaagctatatgatttccttactgtaaaaatgactaactat aatactatattgttgactaatgaaaacatttatcaaattatactgtttctttttgactaa atttggatttttttgttgtcgttgaggatttttattgtgcttttgcttatttgaatgtat ctcctgtgaaatgtctgagaaatgacctattagactctttaataagatataccatgacaa gtggatgttaaatgatatttagtctttcaacatctcaaatctgctaggtctttaatttaa tttcaggttgtttaatagcatattgtcactttgaaatgtgagaattttctaacaaatctg acattgtaattatttcaggtaggccattcagttgattgcatttacactgcagtccatatg cctttcactgcctgtatgcagaactttctccttactcccgccagaaactctgttcccagt aagcaacaatcccgtagttcctcttctttaagtgttattagaccgtctgtctgagccatg agagtgctgtaaattctcaacacgagctgctcagacagctgtttgcagactcttctcagt ttgtgactgattcttcagaaatataaaagctttgctgctgaggcctcagaaggaggtaag ggaacctgggagagaaagcgggaattggggctagagagatgatagaagaggtgaggactc ttgctactcttgtagagactggggtttatctccctgcaccaacattaggcagctcgaaac ttcctctaccctcctctcagtgccccttaagacatacatgcatctgcccatacaaacaca catgcacagcactagcatgctcacacacaaataagtaaataagaatacttaaagagaaga aagctttttttttttttttttttaaaggaatcaatgaaatgctcatgaatagactgctgg tggagatgtaaatgttaaaggttggtgagggtcacacgtgtggaggacacagggcctcac tgaatgtagactttcccagtgggttagactggctggctagtgagcccctcatgcttctgc ttctacctcctagtctgggatcgccccaggattgccattacatgctagcctttttacatg gggagctgaactcaggtctccatgcttggacagcaagcattttgcctacctagccatccc cttagcccctaaatttatagttttcataaaatgtcagtatcacttgaaacaaacttcttt tagagctggggaccaaacctgaggatttgccagctctaccactgaggtgtttccctaacc ctgtgtgcttaggagttaccataaagactttgtcacttctttttgaacacatgaacattg tgcagtttaatgcaaatttcagaacttccttgttaaaaattgaatttatgatagaaattt atttagcttaatatattagtctacttagattagcttatttattgaggaaaattatttgga aactggaagtacattgttttttggatcattttaattggtaacttctgataatggcagtga gtacatttgaattataaaatctatacagtcagcccttatctacctttaaatcctttacag tgtggtcttgtttgcttgttttttgatggaatcatatgcaaggatataattgttgtgttt ttagccattactacattgaaattcagtgtctaggtttaaagtaaaatgtaatgttactta gtatgaaattactaaacattacagttccctcttctgtcacttcagagttactgcaccatg cttagctttaaatagctgaccagaaaactgtgtaccaattgtattgttagtatttcttta atagttgaatttagaatattcactgccaaaagttatttgtaggtaatatgggagattggg taacaaggcaagtaacacagtgaaaaacacaggcattgtacaattgatggcatcagcatg tttctgctggactgggcagggcatctaggcacccagcggggtggattaaggcgaaggaag aagccggtggaacggagtgctactccattcatctgcgcggacttctttactttagatgtt tttgagacaatgtggaaaatttgactattgactggtgttaatgaaatgtggttaatgtga tctaattgtgtggtgattctgtgaacatgagaggcacttgtgaagtgtgtgtgtgtgtgt gtgtgtgtgtgtgtgtgtgtgtagtaagtaggcatggtatataggacttaatttaaaata tctgcaaggaagaagaaaagtcaaagcaatattggtgatattttgaattgaatataggtg taatagatataaaatataaataatatactgtttttttaatgtatattggaaacttttcat gtaaaaattccagatttttacctactgactgttttcctggtaccacaaatcctctcctag catttacaagtcttctgagatattggagagctttacttaaatttttgttgcagaaatgag gctcttgtaaaccatttaacctttccctttgtattttcttcttgttatctgtcaccattg atcatagtaattattctggttgttacgtcttttttttttttaaactttattttgtctttg ttttgggaatataaatttaattctggtggaagagatttgtttctgttgcagtaccttcat tacaattgttttgttttgtttttcgagacagggtttctctgtatagccctggctgtcctg gaactcactttgtagaccaggctggcctcgaactcagaaatccacctgcctctgcctccc aagtgctgggattaaaggtgtgtgccaccacgcccggctccttcattactcttagttgcc tagcatattagatgcttaataaatatttcaatgattgagagaatgggaattagttctaga aatccaaggacatggagtttggtcaaggacagagcaaaagagaatgtttttgaatcttat gaggctatggaaaccttaacctttggagaaaaatgtgggctaatatgtacagatttggaa gagtaagtcaccacctcttgttttttcactctaaaaccttgatatttcatacattcagca tagtcccaacaacaggaattcctctaagttattgtgctgtgcttttccaattgtcttcaa aatgttctctgactttttgaggcaaatcttactctgatttccaacttaacatggaagcgg tcctcctgctcagcctctcagctctccaggacttcctgcctgtgttgccatgactgtact tatttatctgtatgatgtaccacttgagtctggtgtctatgcaggccagaagaagggatt agtacagatggttgtgagctgccatgtgggtgctggaaattgtacctgggtcctctggac gaacagaacagccagtgcatctctctagccttgaataactaactaactaactaactaact aactaactaactaacacacacacacacacacacacacaaaataactatattctccttaaa caaaataagaaggatggcattgtgtttcatttttgtaaatgtatttaaggtctggcttga aatatgttttagcttttgtgtttagccaagtgagagacaggctgtaaagtgtaaggaaac ttctcaggtaaataactacaggtgtcttcttataacatagattgagaatgacagactaag caacatcctaggagtactgaaaacagttgtggtctcatagattccatgcaaggatcctgg gaaccctccatgttgctggagtgaataatttctcactgggatactgtttatgaacaacat gctatgtttgtaaatggaagaatcattgtttaaagatatactagaaccggtttggtgaat ttatatttgagacatttttcttaaagactgttgacagtaccttatgtttacttacacaga taaaaatgggccaaagaatgtcctgccacaaagttgtgtttgtagttttctgaggatgga tgaatggactgatggacagatgggtggatggagggagcaggggagggagctggagatcta accaaggcccttgttcatgctaggcaaacactcaagacctctactacatacagtatttaa cttctcaaaggaagaatttgacattgagttacttatagttaagaaggaaactagttactt tattgaagtaatcatgagggctggggatgtggttatgatggcttgaatgagaatggcccc caatgattcatatatttgaaagtttggtccttagtaggtgaaactgcttaggaagggtta ggtggtgtgggttttgttgaaggagctgtcactaggggttgggctctgaggtttcaaaag ccaagactaggaccagtgtctctctctgcctcaaacctgttgacaagatataatctgtca gctccttttccagggccatgcctgcctgctgccatgcttcatgttgtagtagttatgagc tcaccacctgaaactaagcaagcctccagttaagttcttctaaaaagttgttttggtcct tatgtcctcagtgcagtagaacagtaactaagacaacggtatattccttgcttaccatgc ttaaagccctgggttccatccttagcatcacacaggaaagaaaaatcatacttttaactt attattgataaattattaataaataatgttttctctactttttaaaagggcctgtaggta gacttgattaaatatgcactgtctttttccctccagaacagtattatgctcatttttctg atagttaataaatgtttacttctgttggttagtcttcaagttctgtctgtatgcaacata gtgatcagtattacattgaattagttaacagggaagaaaaatgataaataatgaaataac cattattgcttacattctatttttgttagaactaatttaagagccaagggatattgggga tggtatagggaatgaggctgatgaagcatttcatgcttttgggtatttcattttatttta ttttgtttttcaggacagggtttctctgtgtagccctggctgtcctggaattcactttgt agaccagggtggcctcgaactcagaaatccgcctgcctctgcctcccgagtgcagggatt aaaggcgtgcgccactacgcccggtgtggatcttttattttaggcctttgtagaagcagt agtgatgctgaggctggtcagatacctggctttcattgagagcagcatatatttgctggg gactttaaattgattcttgctttattttgtacacagtgatgtatgttctgagcatattct gtacttattggcggagtaaattatgttagatgttaatgcttggtacctgttcttacacta gcacaatactttatttatttatttatttatttatttatttattttaccttctttagctcc cctacttcccatatttgctagagaggtttattagcagcagttaagactgtaccctaatgt ctctgatgagcatctcaaaaggtagcaacatggctaaagcttgcttttacaagttctgtt gataaatcattttagacacttagtctgatagtcccttgcattttccatgtactgtgttct ttggcttgtctggcagcagcacaggaaggtagacttaacggcagtccagctcaaatgcat ctacagatgagaatttatcttctgtttagcatacagtgttttcagcaggcgagtacgatg ttactgaattattttgcttgtttctttctacagtcgttatttatagaacttttttccccc cttgtgaagtacatgcctgtttctaggtattctgaaaaaactgatttaagtataacatga gttaagtctagaaggacagtgcaaggtgaaggcagaaagagtactgactctttttagctc taaagcagggaagggactgagcgtgggaagggactgagcgtgacacacacacacacacac acacacacacacacacacacacacacgcagagcagggaagggactgaatgtgagacacac acatacacagagcctttcaatgcctcaaacataggctcttctatagaaggtattttgtta tttttagtttcctttttctttttcttaaaggtgtattaaaaagagttgacatcattggta tctgaatgttcattaaattattttgaatatgattatttaagaagactaacatgtttgtat taatgtgactctaattctgggaattcagaaagaattataattactggatacaagtaactg ttatcaggaaaggtagaatcttttaagtataaaattgttagggaatgactaataccttca gtggtgtttgcttatgttttcagtgtagttttcgggtctgtctactgaaatgcaaatgac caagggaaagatgcctcgtcagcttaggttccgatgacttttagttattgcaaatagagc acatagccacatccttttcttctccgtaaacctcagaacacttatgacagcaggcattcc acagctcaccttgctgcttgtctcattcttacctgtagtgaaactctgcagtagattttc ttgttatccctcaggtccaagttcagcatgcagagggaatttcttttaacattgtattca gtcatcctcctgcctctcccaccactattcaaagatttctgctagctactctccacattt tattatttttaaaaaagctgtaagcccacgttgtctgtttctcgttgggttttagggtga ctgactgagaacagttctgcctgtaactgcaatagagaaataaagcgagtgggtattctt actgtgcgtgcttggcaggcaggcgtcttaagtgtagagtcagaggtcctcagttctgag tggtgagctagaggctgtccagtgatttgctcccgctgtttctcagagcagtgctgcagc ggttctctccagatcctgaggctttcttcaacagtgtagaacagtgagggtggggctctt tccggttggctgttgaaaaagcgtccatttacagagagaacaggttggataggctaagct gaaggcgtaggcaaacagatccaaaactgatggaagacagaaagagtggttagtatgttt tggggtgtcttgagcttgcttctttgctaagttcttgagctttaatataaacacattgaa atattaaagcactttacattctttgtgtagtagcctggagacttatgcttttcacagttt cttggtgatgagtttgtggctgctatcttctatagtaaagttggtttttcatttgttttt aatttgcttttagtggtgtatctttcagccagagagctgcagaatgagtcagttagccag ttttagtatatatgggacattactagttgtgattttttaaagccagattctcttttaatg atcaagtcaatgtgtggctgtggctgcagcaacattatttcttttttaatgaagaggttg aaccgttcgacttgctgctccgatcttttcccttgtagctatacatcagaacagttgcag agattttccatcgaatgtcccaggcaactaaaggtaactacatagatttatttcaaataa gaatacacagttaaaatggctgcattataagttgggccaaatgattaaacataaaagacc aattaagatgttcttagcagttttcttgaccttgcagtagctatccaatatcattttgtc atcatcagattgtggcagtggaaatgtactgcagtaattgattttgtaataggatcaggt gatttaccagcggcactgagaaccacttgcttgctctgagtgtgcagcactctaatgact gtgtgattgcagcctagagctgaagtgaggtggctggccacccacagtagcagcgtttac tttagaaatcacaagagtactaggagtttcttgtttgttttctgtgctatcagttttact tcatgtgttcatgtgaagtcttagaaattacgattttctttttcactcaccagttcataa aaagtttatcggtttgactaacattaagtataacaaaacacattcttctctctctacttc tgagctcagcatcaacatactgtgctgctgaccatgaggacacatgtctaaaacataagc ttttgttaactattgtacattatctgcattaaggaaaatattcttaacacctgcaaatct catcatgtttcaactcttatctgtatggtaatactgggactagaggaaactattggtcca gagagttactagaaaattgttttgtgttttattttgtgtttggatttctcagtagtttat ttagcaaatctggactgtataatttcttggaaatatccagaagaacattaagggcaagtg taatgtttaatgatactacaaattaataacttggtatccaattttatttaatatccaaaa tttataaaatggctatgttttaataatgtttgtgcctttaaagcaaataattggttacaa atatgtgtgtacagagctcattcttgatgcggaatgcacctgctgttgtaggaatgtgag accctgtgttgagatcctggctgctgagattttgctacaatgatcttttgcttagctttg ttgtattagtgaaaaaaaaaattacacgctttccctccctcactcctttccttgccagaa ccaccaactcttgtgcttagtatgtaatagtgttttgtacagaaccacacacacagggaa aatgacttttctgtcagtccttagatatatttttcctgttagccttactatatttcataa cagttaaaattagatcttgcagaatcaagaagagttctgccattgcatatgctatgtgag gcgcttccctttctgataagcccagaggctagttcttaaactgtttgaaggcttggtgtt tcttgaaaggtgaaaatatgaacagtcatactttggccaaatccagatacttaaaaaggt aattatagttatgtgttctgttgctaataatgatgcaatatcaacatggagtttgtagtc tttattcttctttgccaacaacagaaatccctctgtatacattggtaatgtgtacataag gatgttattactatttgagatttgttcaaggacatgtctaagttcaatattgaaaatgtt aaatgatgtaccatcaccaaatcaattactgataaatatttcactatggtagtgttcatt aaagcccctcccccagcattgtacacttgattgagcaaattggaacagaaatatgtccca gtgtgtgattgcttcttaaaataatgattgtgaaagacacagggcactgttacatcttca atatgatagggcttcctttagggttaagtgggagatgtatcctgggaagtccttgcccca gctgggcacactcttccaaagtgcccgagatcaaacacctggattttgtttgactttgtt acaaaggaatttgatgatcttgacacagttacatatgtatgaacactataagctccctta aaatcctcattagaaagaaatgggcgtgtcctgtccctttagcactggggttggaagctg tagaagttggactttaatggaaagaatcagtgtgcatgctgtagggacaactcctttcca tattcatgcctttctttttgctgagactactgttttatctttttgatatctaatattctc taaatagcatatttgtagtagcgtttaaaagaccagacctagggctaaattctgacacgt gatttttttttccctaggcatttgatgataataatgttgttcggtcatttgttcagtatt tcacatggtagttttcagtggtcagagtttgggaaggcactgttgtttccagtgctccag caagtgcacctggcaggtaagcttgtggaggcgcctgcctttgcggcttaggatggactg ttgccttcccagagatggggatgtggtgccgactcactcatccttcaggaacctgtgttt tggatcatgctgctctctagtgaggactcctttgtcagtcatttcacttgtgagattgga actctgagtatttttaagacaaagagactgaggcttacaaaggtctcattcctaaaatca gataactaggaaatagaataactaaggttgaagcactactttaaaatactttctttgaca acaatgtgataaaacactatattttcttacactttaaaagaattttctttcagttaagcg taactgcagagatggcaacaaatgtctgaagatactcatgtatccataaactacttcctg tactatgagtgaattttcaggtgtgggttagattttatcaactgcttctctctcgaatga cagaaaagcttggcatttactaattgtacagaagaaaaatataagttcatgttaatatgt gaagaatcaattaacaaacaacgggactagaaagtacagtttgtccttctataaaactat tatgtagtgtgagttggtttgtgcattcctcactgagggatttagctggtatagtggtaa agtcacttcagtcccccatgttcttaagtaatgtaaatacatctatttcagggtttctac tgctgtgataaaacaccatgttcaaaagcaacttggggaggaaaggggttatttcagctt acagttttacatcacagtccatcactgagggaagtcagaacaggaactcaaagtagaaac atagaggcaggagttgatgtagaggccatggaaaagtgccactcactatcttgctttcca tggctttcttataccatctaggcccaccttggggtggtatcaccagcaataagccaggca ctgagatatcagtcaagaaaatgccctgcagacttatctacaaataaatcctgtggaaac aaagactgtagcttatgtcaagctgacatgaaaattagccaacacaccatcagaagtaag tttttttcacaaagagggtattatcaatatataatggcattaagaataaaactgaaggtc cagagagacacttgctatcatgcccaattacctgagttcattttctacagcccacatgat aagaaagaaattcagagagactcctgaaattgtcttcagaaccttctgaatgccacatac ctattttcagaacctgatcttgcagaatttccttctttttcagagaaagacagattgttt attatttgtgtgaatgtggaacatgtgttatctaagtatggatatgtatagaatgtgtat acacaggtgtatgcatggagggtattaagtatctttctcagtctatcttacctctatatt ttgagataaagtctctctattagtagctttccatgtcctgaaactcaatatgtagacaag gatggcctaagactcagaaatctgctcacctctgcctcccaagtgctgggataaaaggca tgcaaccaccaccatgcctggctcaagccagtgtttcttactgatcataccaatttagct acacattttggccagcaagccgcaggaatcctcctatcgccatctctctagtcctgggat tacagatacataccagggactcaaaacccaggtcctcacactcatgcggacacactttac ccaccaagccattcaatttgtaccatatgtaacagaactgcctgtgttacagctttcaag gatctcagggatagccagtgcttcatttagacctggctttactgtggaatgaataccatc ttccttgagatgaagttttattcctgatgttcatcatgttcagtcaacctcaaatagtgt ccatttcagctgtagtctaagaaacactaatagagatagaatgaaatattaaaagaaaaa acactagaaatacagatttaattatagagctaggatctgtggtccttttaaaacaaatta aatttaaattcttgtatacgctgtctacttttcaaaacaaagtctggggctggagagatg gcttagtggttaagagcactgactgctctttcagaggtcttgttcaatttccagcaacca catggtggttcacaaccatcggtaatggaatccaatgcccttttctggtgtgtctgaaga gagtgacagtatactttgtgtgtgtgtgtgtgtgtgtgtgtagtgtacacacacacatat aaataaataaattaaaacaaaacaaaatctgagtcagtttcatcgactttatgaacatcc catatgagaaaaaaaggctatccattaaaatgatatattttcttgggagccatctgcctt tagcagaacaaaattgaaattgaaattattttcttcatgaaaattacaaattaccaatga gaagttatgggtaccattcctggttataggcaattctttctttaactaagctgaaaagta ataaagttgacttcaatatttaaatctattgattacatatacttccctcatccaatctaa atcataactttgatgatataaatccttaaaaagtaaatcattaagatatatacttcaaaa gtaccatttgtgttgctataaactggaatcacttaatcaagaaataagagagaaagaagc gtgtagcacttcagaacacacgaggggcagcagcctgaagagaagacgtacccacgcacc aaacattcccagctcacctgcctgcccagattgcatcctgtccactgcacaggatttcct ttgccttggaactgtcattcataccattttctgtcaagttaagccctcttctctaacatt ttgaatcagcttctgggttagggattaaggttagtaactattcccaaactccatctttct ttttcattgcttacatttacatgatttaacttctcaaagcctcactataaggcatacgtc ttttctgttaaaaattttatatgtatacatatataaaatataataaatatatagtataat atatatgtttgtatgtacatatatatgtatgtatatttcttttggggggcatggtttctt tgttagtggtgtggtcttcttttttgtttcttgagatctcacagtgtgaccctgtataat cctgcaactgctatatagaccagctagccttgaactcagagatccacctgtatctgtctc ccagtgctggcatgaatgacatgaaacacaccacatctggtctctgcaaaaaaattaatg tggaataagcaaacatatatctttcttacagaaaaataaatatgtatcagtaattaaaag caatttccactctggataaaaacactaataaaaatcaaaactacaatctgcacagaataa aatgtttataaacaactagaactcatcctactaaactaactcttaactaagatgactgga gttttcagaactgggtacttgtcaacatgggtgatgtgccagtatcaaaactcaggtttt ttgtaactcctttcttttcccatatcatttacaaactaatgacatcagttcctttgaata tcttccccacaccagaaactagacccaccaaccgttcattccccttctgctcaaaagctc tacctccttaacctcagtcactctgctatgcacaagtctatgacttaaatgccaagacag accagggtgaatggtgaagtctcacaaagaggagagaatttgtaccaatttgactattca gatttaatttttttaatttgattttcttttcatttctttgatttttgtttttaatgaaac cctaataatagaatgaaatgttagaaaagcaaaaatcagtattaaagtgtacattgagaa aaagctgtagtgtcctgactcccaacctctgagtaactaaggactttcaagttgctccta aagttaacctggatacaaggcacatgttaaagggtgcctctttgggtggcttagcttaca cagaagcaacaaatgcagaccttagattctagaggatgccacttactgctcttaacatgt gcccatggaagccacttcccaattcttcttgccaactcagtattatatatgtgtagagga aatttatcataacatccagagtaactgtgtgaattgaacacaagacaacacacacacaaa acaaacaaacaagaactagtcatgtctgaagctggaagtaaagtgtcacggctctgactc ggctcctttcccttaaacactgtttttgcacccactgccaattcgtgaattcagctccgc aacacttagctccttgtctccagtactcgtccctgcaaagctgctgcttttggcttccct gccctgcatgccttccccaggctctccactctgctctcaccgtatccgctgccaccaaca cggctgattcttaaaactcaacgagatgagccatcccatgggagcaagggcactgcttcc agggcagattcctagaacttgtggcaaaaacaaaaccactggctccttctccactgctgt cttccgagatggctcgcacactccccactccaaagccattaaagggcgcttaagaacctc aagcagcatcagccacacctgtgctcttcagtgttggctttcctaaagggcccagcagct gctccagggcagtttttgccttttctgtttcagtaattccagttgcaccggaatttaact gtaacctaacaaagtaagtgacaagaatggcatatgaatcgccctcgtagcagacatcta cagtggttggataaaatgaaaaccatttgcttattctatctcaacaccatcaacagtctg aagcacgattttacattttacaaaacggattctttacaaaaacaatccttaagaaaagga aaacttgcatgcaacatgcagaacaagttaagaatgacagtgtgttctcagaaccaatgg ctctcaaaagaacccagggcttgagagatggctcagcagttaagagcatatactactcta gcaaaggacctgagttcaacatctggcatccatgcgaggctgcttagtgggatccaacat tctcttttggccgctgtagacactgcacgtatgcagcgtagactcaactcatacacataa gtttttttttttttttttaaacaaaataaagatgcctgtatcccattacacagcttttgc cagtggttactaatatagcgtgaatgtgtgtacgcagatgtgcatgtgtgtgtaggcaca cctaactctgcacgtgcatctggaggccagagaacaacctgagctgttgtatctcctcag acacagcaacagcctaccttgtctttaacacggggtctctggctggacctgactagatgg ccggtaaacaccaggaatcttcttgtcttcttcccagtgctgggagctgcccctggcctg cccgtgggtaccgggcatcggcgtcaggaacacatgcttgaacaccaagcacgtgacagg ctgagctatctctgcagctctagtttttacttccccctcctttctgagtgaggcacgtga tccctgaagcactactccaaaggaggacatgcggtgagatatgtttacaagacaaattac catgtgactgctgaataagagggtcagcatgtcacctcattccagtcgctgtgataaaac gcccttaacaaaagcaactttttgttaagagaaagggctgattttggctcacagttgatg atgggctgtcatgaggggaccctccagtgacattcaggttacattcttgctgcccagcaa tgcttcccctttctgttcagttcaggacccagcctgtaaaggaatgctacttcctaattt tagggtgggtctttagagcctaagttatcagttaacctaattgaggagacaggtacaggc aagggcaaaatctgctttcctgggtggttgtaggtcctgttaatttgacagtattaacac tttccaaatgctagtggaatcagattttccagacatggtattatgtacacatactggtgt gtccctgcatctttgtgtgcacatacacgtagaggtcagaggtcggcctctgaagttatt cctcacacactgtccaccttgttttgtttttgtgacaagtgagctccagggaccttcctt ctgtctcctgctgggagacttaaatttccaaataggttttaagatcagaagcactagtcg ggtcttggggtctgggcattgagcacaggtcctcctgcgtggcaagcactttactggttg ctgtctccagccctacttcttagtaagttagaaaagtcttctgtttatttacaagcacaa gtagaaatgattttctgacaccttacagtttgaatgcctataggctctcataatagcccc cctccctcactggttgagtagtgaataaggaggtgtgttaaagtaaattaactgacttga agatgaatgggaatattgtgccaggtagttttcagtagacacattaggacagacatcacc cattttacaggcgatatgctctatcagtttctgtatctcattcttacccttgttatccgg ctcccttagctcttgcctgccatctgagggaatttcaaatgggtttacagaagagctgag caagcagtttattacagtaaaagttcctccagacagctaggagagggctgacaagaggga tggcccacagctctcaggctatgaggtttgcctctccccccacccccaccccccatgtcc tctacttatgtccccttcctcccaccccttttcctctcccctccttgagccagggttcta taaaacaggttgtttttatcttctcccagtgtgtgcactcattgctaagttgcttgtgat ctggtgtcctgatgataggatcacatgactgtttgtcagctctgatctcctgatgccaca gagctgagattaaacaatgcagctttaccccctgaccccttccaccactgttgactgtga cttctacccatgcagtaacaacttggaataaaagtaactcagataacattgacctgatga agctgtgggggagtccttgtcagaatgacagatctcatcatcgtttgcattttgctgtca tcttttcttgagcagtggctttaaagtggtgatcaggtgattctattcctaggcagctta gctcaagcaaatttaactttctctgaagcagtataaattccaccaaagacacaaagaatt cttataaagctccaaggtaaaattccaggggacagtgaagcttcaacaacaagtgaattg aagaaaaatccttgttctagcatgcctctcatttgccttctaaaaagaaacagaagaatg gggaagagtctactaaaaactgaagaagtatacatatgtaaagctcagagaatgtcagtc aggataaatacaagtaaactcactctcagactcagcacacagtaactgcagatcagtcag cctaaagaaactatttaaactacaactggagagcagaagtaatttacctaaaagaactag gtatttcactgcattgtgagtagaaatagtggaagcctgatgatggtaagttcagcttgc tgagaagggtggccaaatgaagacctgctgtggaggcagggaggctatcttagttagggt ttctattcctgcacaaacatcatgaccaagaagcaagttggggaggaaagggcttattcg gcttacacttccatactcctgttcattaccaaggaggtcaggactcgaactcaagcaggt cagaaagcaggagctgatgcagaggccatggagggatgttctttactggcttgattcccc tggcttgctcagcctgctctcttaaagaacaaagactaccagcccagagatggtctcacc cacaaggggcctttcccccttgatcactaattgagaaaaagccttacagctggatttcat ggaggcatttccccaactgaagctcctttctctgtgataactccagctgtgtcaggttga cacaaaactagccagtacagaggctgatgtgtagattccgtctgtattggcttttacccc tcatgtgactgctgcttactataccagaggtgtcagaggaggagtcttcatgctgggtta cctgagccaaaggatggcagctcctgcacagactacagtgtctctggaaagcactgctct ttacagtctgcctgccagtctagttttgcagcatttggtgtttggttaccacgggtttgt gtaggatttgtgtcttagcagaaaaaaagcaaagggaagctgtgggagtcaggcacagca acccccagattacacggttccttacagtgttcttcccttatacctccattgtaagagggc tgtctgtaaagtgggagttgctgcttctactgatagaccactagtgtctggagccaagta aatgcaagtctaatgtgtgctgagaccacactagaaggctccctgattttcaactctata ttttcataggagaaatctcttagaactttttaatcaaaggcaaacaggatgatggagttt cccatagccgttattgattatcttaatttacaaaactgaaatctgaaatgccccagaatc caaaattgttgactgtcatgttggcatacagaaagtttctgatttgggaacagttggact tcttgagattttgaaataaggaatatgcagcatacagcatgttaaatgctgtgtgccaaa atatcggtcagcactgtatatgtacactcttatttaatctttaatacttctataagacaa agtacaatcatttgaaatcctctctgcagattagacagttgaggttttaagacactggat aacttgtcccgttttgtaggtagatgctggcacaacttgtgtttgtagctcctcactacc aatccgggcctcttatccaccaagattatagcttgggtgttatgtagctgtgtatttccc catccaatgctttaataacatccagcaaggtttatatttttcatgtcctttgttcctatt tatttttactttcaacataaaacagctctgctggaattggaatggcctggctgtggccac agtattgtataacttccacagccttgggagtgaaattcaggcaaatatgttgcagcaggt ggtcagtgcttaaaattatttcacttgtgatacagcgtgtgtgtcagaggtttactctaa cagtaacattacgctttaactgtacagcacctggcataatgtaaggttcagttggagata attgttattttttttagttgtgtggggaattagaaggcagcaaaataagaaagttggatg tgtttgtgttagaattcaaaattttattttcattattttgccttaaaagtaattttagat gtcaaagtacttataacttatgtaatacagtgctttttaaaatttatctgcagctttaaa aactctttgtaactggtaaggaagcatgcattagttactgttctgctgctatgataaagc accacgatcaaggcaactgatggaaggaagggttcatttagacttatgttctacaaggta aggatatagcagcagcagcagcagcagcagcggagagttcacatctgaaccagaagtggg aagcagaaagagggaatgcctgagtttttgaactctgcaagttcagttgcagtgactttc tccagcaaggctactgctccccaagcctcctaaaacagggccaccggctggaagccaagt attcacatgccatagactagaaggggacatctgactctcaccactaccaagtgcagtaag aatgaatgcatgagtttggggtgtgcacaattacaaattaccaaaactcattagtcctga tacaagttctaaggaagagagttgtgtcttagttagggttttactgctgcgaacagacac catgaccaaggcaagacttataaagaacagtatttaattgggctggcttacaggtccaga ggtttagtccatcatcatcaaggtgggaacatgacaggcatggtgcaggcagagctaaga gttctacatctttatctgaaggctgctagtgcaagactgactttgaaagatctgcatgcc tttcttctaaacactgggattaaaggtgtggtccaccaagcctggatttaagtttttctt tacctagaacttgctctgttctaggctggccttcaactcatatctatttgtctctgcctc ctgggattaaaggcttgtactaccacacctggacctaaatttagatgggtggaatcttgc cccaaggtcacttctcccttaattcaaattaatctccttgagcacaggattcagctccat ttcacttcctggtgtccctttagtactcaaactttatattttgtatttttcctttctcag cttgctacatttgttcaaatgttcttcatcagacttaaccagagaacaaagtctgttggg ctttgttgggacttcctttgtcaatgcaattattataaatctctttagtcttagtcttag gcagtcttttcagacaatggccacgttcaccaaaacaacacaaaacagtctctcaccaca tattaaagttcttctccactgaaacctcttgggctagcatgcctaaacagttcaaatgac cctcagcaccagtcttccatattttactaggttggctcattaagccccacttaaagcatt ccttggcttcccaaacccaaagtctcaaatccacattcttccaaacaaaagcatcgtcaa gcctatcacagcagtgccccaatccctggtaccaacttctgtcttagggttttactgctg taaacagacaccatggccaaggcacctctcagaaggacaacatttaactggggctcactt acaggttcagagtttcagtccattatcaaggcaggaacatggcagcatccaggctggtgt ggtgcaggagaagctgagagttctacatattcatctgaaggctgctagcagaatactgac ttccaggcagctaggagtagggacttaaagcccacacccacagtgacacacctactgcaa ccaggtcacacctattccaacaaggctacacctccaaacagtgccactccctgggccaag catatataaaccatcacaagttgtcactgtcttaatcttaggagggagcttttccacaaa aggagtagtcacagatggtactatttgctgttgtaagttatgtcttgtttgtggtggaag acagatttatacgagtgacttagaaataacctgcaccagctaagcctctggaccagcagc cctaagaacttgtttcctgttccactgcctgggaatacttatctcagagtctagattagg tgaatgtgccccctagtgttagagtgggcactctgactgcttgctggttaaatggaagca tctttacccatttaccaactggaacataaaactggctttgtacctgctaatattttctgt attcttagtatcttggttggtagattaaggaaatggatctatagtattttctgttccata actccttaattgctagtgtaggtaatggatagtattttctgtcttttctttttcttagtt tgaattagtgcttggatttttgcctagacaaaaggagtaacttggaatgaagaaaatgga ctcaaacttaaagagctgcacactaatcaatccttcgaacgcacatggaaaccagagacc acccctactgtcctcttagttacacaagtggctgaggttagtgataaaatcctctgcagc ctccactgaattcaagctgtgaccaacacagcattgtctgtatggaaactggtgcctcca agttggagagtgcttacaagtgctgccttgttaggttggtgcagaagtattgttctgtaa cctgaacactctgaaaatttcttgtgtgaaaaatacagattattacaaacaggatctatt ttaatgattcattaagacactgttattttatctctacacaaatggaactctttgtaagag aatcacagtatttcttaccatttcttacctcataaaaccagtcagtacaactgaccctgt gtcaacttgacacatacatccatcactagtaagctataatctttcctttcttaatcatct ccagatctataactttaaactccctcattttttacaaattcaaacacattaaaattcccg tccctttaaaatagccagtttcttaaaatccaaagtcttttaagacaaagtctgaattgt ggattcaaagtcttaactgtagactccagtaaaatacttttttacttcaagaggaaaaaa aaaatcagggcacagtcacagtcaaatcaaaatcaaactccaactgtccagtgtctagga tctactcataatcttctggacaccttcatagggcttgggtcacttcttcagttttgccct ttgcagtacacacagcttgtcttctaggctctggttggctccattccactgctgctgttc ttggtagtcatcccatggtactggcatctccaaaactcaagcctcaacttctgtgaaggc cccttcagttctgggccttcaactgccactgaggcggcaccctcaccaatggccttttct ggccgctcccagtgccaaacctcagctgctctccatgacccctttatatctttaaaactt gtatcgtctgggtgactcatatattcctatgtccggctgccaacatgaggtacaaccttt gttgcctctggagcacagcttctctgtgctctcagaaaacacttctcagaagactttatt tcattaatactggtctttctttaatcggcactaatttcttaggtccagctaaccagcaac aaatgttctagggatgtaaaggtttcactttaagtagttctggtgtcttgttagtcacag ctacagctggttctttagccccagctggccagaaccagggtctaaatcacagtaacaaat ggcccggatagtctttaatcttccctctgaaacttcacaagacaggcctccatctttatt gctctcaacattcttttttttttttttaatattttttaaagatttatttatttactatat gtaagtacactgtagctgtcttcagacacaccagaagagagcatcagatctcagtatggg tggttgtgagccaccatgtggttgctgggatttgaactcaggacctccggaagagcagtc agtgctcttaaccactgagccatttctccatccctcccacccccgcccctctacattctt atcttacaggttccttcagaacatcccactgaggtgtcaacactcaatggttcttctagc ccaaagttcttacatcctcccacaatccttcctaaaacatgtcaggtctcttacagcaat aaccttctgtattagtcagggttccctagagacccagaacttaaggatagtctctatatt gttagggaatttgttgatgacttatagtctgtagtccaactctccaacaattgtcagcag tagctgtgaatgggaagtccagggatctagcagttgctcagtcccacaaggcaatcaggc gaagaagagagagtaaatcttccttcttccaatgtccttatgttggtctctagcagaagg tgtggcccaaattaaaggtgtgtggctcacgcctggatctgggacttgctttgtcactat gcctcaagagctccatgccaagatccaggtcagaaacttgtatctcccagattaggatca caggtgagtcttccaattctggattgtagttcaatccagatatagttaagttgactacca gaaacagccactacaatcaccccttgtcaacttgatacagataacatctcatgtccacat gaaacaataacaaagccaagaatacacctaacatgatatacttactatccgtcgtacaat tgcaaacacatttgtaaatttacaatggggcaatgtcccttgagaacattcttttagtgt ctcaacttaaataacaattgatgttaaagaaattggaagaaaaacaaatgtattctaaac aaaataagacagaagcatttacattacttgataatcctcatttctgcaactggttacatg gccttagctggtatttataactaccttcctctactacccattctgtatttccttcaccct tggcaaacacctcagcaggtcttggctcttttcctggaggattgacccataccttcattc ctgatgggtctgcatcctttgtcaccttgcttggattaggctgttgtagtttcccattga ctttaatcacagggcatggtagtactaagagacgccctaaggggtctcctacattccaca cataatcctgcttacctccattgtggagaggcaatccaatttccccatggtaatctggat ctatcacccctcctaacactgttattcctttcttagcctgttggtttaagggcattagaa gcccaaaatgaccagggggaagtctgagctttcagttcaatggaatgtttgttgtggttc ctggtatgagcactccctgctctggatcccaaacttctaggccagcagaacctagagttg tggggacaggatgcaaaacatttcctataggttcactgggagtgataatgagtggaacta ttcccttttccaccccttgattcctggacccatggatcctgactatgggggaaactctac catatattggatgctgattcaaagcatatactgccttctgaagaactctgccccagcctt ccatgctgctgccacctaattggtgctgtaactgtgtcttcaaaaggccatttcatcttt ctgtcagaccagctcctttaggatgataggaaacatggtaagagctgtagcactttgctg gctgtgaaatgagttccttggtcagaagcaatactgtgtggtataccatgacgatggata aggcattctgtgagtctgtggatggtggttttggcagaagcattacgtgcaggaaaggca aatccataaccagaaaaagaatctattccagtaagaacaaaacattgtcctttccatgaa gtaagtggtccaatgtagtcaacctgccaccaggttgctggctggtcacctcgaggaatg gtgccatatctggggctcagtgttggtttctgctgttggtagatctggcattcagtagta gctgtaaccaggtcagccttggtgagtggaagtctgtgttgctgagcccaagcataacct ccatctcgaccaccatggccactttgttcaagtggtcattaaacaacgacatggatggct ggggaaagaggctgactgtccacagaacaggtcatcttatccacttgattattgaactcc tcctcagttgaagtcaccttttggtgagcatttacatgggacacaaatatcttcacatcc tttgcccatttggagagatttatccacatacttcttccccagatgtctttctcaacaatt ttccagatctttccaggtccttgaccatccagtcaatccattggctatagcccactatgc tggtactaacttgtcttagggcttttaatgctgtgatgaaacatcttgacaaaagcaatt ggggagaaaagagtttatttgacctacactttccaatcatagccggtcattgaaggaagg caggacaggaactgaagcagagcaggaacttgaaggcaggagctgatacagaggccatgg agagatgctacttactggcttgcttcccatggcttggtcagcctgctttttcatagaacc accagcccagggatggcaccacgtacaatgggctgggccctcccccgttgatcactaact gagaaaatgccctacagctagatatcatggaggtatttccgaatctgaggctgcctcctt tcggatgactctaactcttgtcaagtcgacgcacagtacagcgagtaaggagagaaatcc agacaggagtttttaaaatgggaagtaggtgaggtaagagcagcctggagctagccactt gttgcttcatatttgacaaagattactcaggagatgagcagagaggtaggctctatagag gatacacttctcggttccaagatgcaaactatacgaagacagttttctttttttgtttca caaaaataatgttaaatgattagctaacaattaaaatgatagctaagttgggagtcccat taaagaaacagactcctcactggttgctacaaataatgtgttttccatactgcagtgaaa gatttattttgaagttcagaacgttcctgcaaaatgatagtgaggcacagtggaaagaaa atctaaaaggatttgaatccatagtggttactttctagttccttatgatgctgctgagca aggctgtctctctctctctcactctctcactggctcttagaggtttcaaatgcttcacct atggctgttgcttgtatgaccctctcatattctgtcactagtagtgagctgtgggcacac acctgcattgtaggggctgtgcatttgagaaagcctgtcactttgcccattgaattggga aaccatgagtctttattctgattccctgatgagatttttatctgatggtactgtagggga atccttaccactgaggacacgggctccaaggccaacaccgtccttgaatttaacagcaga cagtaggagagtcaaaaacagatagagaagttttgtattatcttcagttttacagactcc ccccacccaatacttaatatagatttaaaccaaagtactctaactaaatactgaaagcac agttaagctctgaacaatacatacacttatatacccaacacatcagtcaagatagagatt atcaccctagaaaattctttaaggatatattttatgtgtatgtgtgtattagtatctgag gagaagagggggttggatcctctggagctggagttacagctagtgaactgctcaatgtgg gtgctgggaactgaattctggttctctggaagagcagcaaatgctcttaactccactgag tcagtctttccaggccttactcccaaattcttttaagccccatttcagtcactatcacac ttagaaaaactctgtactgtcttacatcattgtaaatagtttaaaattaaaaaaaatatt gtcagtggaattttacaatatttcttttgcatctggatatgcatgcatttacattcctgt gcatgcacagaggccatcctacccttttccccccaaactcccttgacacatgctctctca cccaatttgaagctggactagaggccagaaaacccaagtaaactctttgtctctgccccc tcccacaatgaacttggtgaatagattaggctggctggcctgcaaaccccaggcatccat ctgtcttggctgtcatttgtgggggaaagttaagcatgtaaatgtttctaatctgccacc aaggtattggcatttcatgttcttgcagtgattcaaagggtagacaagagtgatagagat acatggtagaaatgactagtgatactacttaaactgagttcaggtgtgtgacttgtctgc tttattaagcagggatagggatctgagagtgattgtggcactcagaaggacacatttaat tctagaggctttgagcccatgatcatcatgccaaggaacatggtggcaagaaggtactgc agtggatcagatcagtagctgagagcttacatgtgctctaggagcatgaggcagataggg agactgagaagtgggagtgctgtggggcccttttccaaacgaggtcacacctcccaaccc ttccttaacagttccatcaactggggaccaagtactcaggtccaagggactattgtcatt cacaccaccacaagcactaaggacttactttgccctggtcatttggtagaaagttaccaa cgtgatgtactgtctcatccaaacctgccctttgttccttctttccctcttctcccttcg cccctttcttttccacacccttccattttgttttcatttgtgtgttgggggtgggtgggt ttggttgtggtttgtgtgtgtatgcatttgtatgtggggacagaaggcagttttggggtt tcattctaccctgttcttgtcacacagtctcttactgagacttggggctaggctgtgcag aacttacagacaagcctgcctctgcctcacaacctcacaagcactggccttacaagtgca tgccaccatgcccagcttcctacatgagttctgggtaccaaactcagtttgactggcaag ttctttaccagctgagctatgtgtctccgatccagtgtacattttgtatgaataggacat tttcatgcataatgacagtatagatataaaagcaaagatgttggcatcaatatggcattt cccagacctcctttcagttaaccagttatctggttaatgtgtcttgtgagagattgactg caagactttgtgttgcatttgttaggcttctagaatagttcattgatctcatgtgctact ttcatccctttcaccacttggaggtgcttctgattctgtacagtagtaactcttgcctca tgttcccttagtagtggttctagttgtgccttactagtggttcagtatcagaagtgccag agaaatgatgctgcctttgtcttgctgcttagggattaggtccatcagcagggatgcact tttatttattgtcagtctgtctacatgagaataaaaacattcacatctttaaaacatctg gctagttcaagattcaagtgcttgccttgcaaatctgacagtaagtttgtgcctcagaat ccatagaagggtaggaaggcactgcttccacaaagttgtcctctgcccaccacgtatcat ttgtagcatgcacatgcccacatgcatattatacccagacacgcagtaacaacaggaaga acacctgcatggcaatggttatccacagctcatggtaagtaagttagcgtggtccctgag ttcctgacagtcagtgtctgatcagaagctgctctgtctcccaccctgtgttttgggtgc tcattgaaacctctttttctctattgcactcaccccacaaacagtatcagctcttactaa agttattaaagcaagtaatagagattccattttttctttttaaacattatacattgtctt tattacatcattagatcttatcatagatttctgctggcctggaatgtgccagaataattc ttacacacttcttattaagaataaaaagtcatatttaaatcatctttttggggggaggtt gcaactccctggtaattaattcagtgtttggtctttgaaatcaatcccttatcttttcat aaaattttcaaactcaagaaatttaataaaataggtttagctagtttgagccaaaacgaa gcttaatttttcagaatctcggcttagatgacttaaattaaataaaggccataagttgat tgattaattttatttgtagcgtgacagtttattgatagtattgcatagttctgttactta gagtggtgccccagggaccaggaacagataaccagactcgatgtgattataggactgagc aaacagtgctgcctgcccacggagcctgtgcagtgcggctcttacatggctcctcctctc ccaatctctgccttatacttttgagcctcgtgaatagaaactatttcacctgcccttatt tcctgccacacaaataatgtgggtcacatggccaggtgggttagttggaaggtctcaccc tcaagtgacaaggccaggccgagcttgtttgtcactgctgctgagttagaaaggctcctg tggtatgcaatttgaaaacttggcataatgcttatgcttccatatcaagtcaggcttcta tttggccaggtcgggaataggctttttctcagttactctgtgacaggagatggagcacct gcacttgctggtccccttttttttgctctcaagccgtagcagtggtagtatcatccgtgg cctcttagaatgcattgcttgtcaaaagcttatattcccattgtatccaagagcctcagc tgatggaagaaacagctgccatattcttgatgctgtacagagacagacaataaatatgtt acaggctagcttctgcacccttcatctttcaagtctagagcctagcccataggatggcat ctatatgtaaggcaagcctttcttctttagttacatatttctggatgcaaccacagactt acctggagagatacatactttaatagtgatcctaaatccagtcaagttgacagtgatgat gattaattatctcactcttctggaatgcccattttatgtccgtgttaatatgtttcttag tcagtgttctgttgctatgaagatacaccatgatgatggcaactcttaaaggagaacatt tcattgaggattgcctacagttcagagggttagtctattatcatggggaagtatggcagt ggatagtagacagagtactatatgtagctttagctgagagttctatatctggattctcag gtatcagggacagggacgaggatggacacacacacacatacacacacacacacacggggt ggggagagggagggtagacagacagacagacagtgatcctgacttaggcttctgaaacct gaaagcccacccccccaaacccctacctcttccagtgcacacttcctccaacaaggccat acctgctccagcaaggccacacctcctaatccttctcaagtagtgctactctgggatgac caagcattcaaatataggtgcctgtaggggccattcttattcagaccactacagctatgt atcactgaggatcttttcagctctttttgtggttcttgattttttaccttaaagttttta cttcctgccatgggagataaagaatcaaaattactaaaaagcaaaatgagcaataataat ttgaaataaaagtttttagttatagtattagtagtctgtactaaagatgacaagatttat ttttttgtctattttgttttgagacagtatttattatgaagactaggctggtcttaaatt cacaagtttctgagttctaggattacaggtatgagctattaaactatttgctaaagagga aaaatatttcatgattttatgctttataaaaaactaaatataatttataaataaatttca gagtttagaaagcatttatctttgtggtgctgggatgacatagtaagaaaggacagtgcc tttctgatatggcctcacatctagtgggggaaggccatacatataaatgtgggcttgtgg atccataggcacttcttatgcagcaaggcttattctagtgcttctatttatttagtatta tgggaagaggcagggcaaggggagaggggagcttagtgcaggggcttttactgattttag tggactagtgggagagtgtacgtatgtttgtgcaggtttgtatgctatgcagagtcctga agttgatgctgggtgtcttcctctattgctctacaacgtatattttgcaacagggtctct cagaaaactgggtgctctagattggcaggctaacaaataccattgctcccctgcccagct ctgggttcatagacttggattggaatgctgtagttttacatgggtgctggcgatctgacc tgagatccttatacttgtgaagcaaatactttacccactgagccatattatgagcttacc tgtgaccttttggcagttatttgtggacatgtacaggactcccccctcccaaaaaaactg gacccaattggcatgcatgtgttaataagcaatgctttaccttcttgtttcagctccaca tcataaacaagtgtccttttcatgtctatactgaaatacttgcccatatttgttttcttt ttgttgttgattatgttgtttaaaatgatccccagtcatacaaccaaattgccctctaat atttgtaagttccaaaatgctttgatactgccatgtgaaaaaaatactgcagtttagcta agtctcgtcccgacctgagttgtattatttgggcatgagttcagtgttgagtcaatactg tacattgaataagatgccttttggctggtaggtcaacagtgttgtggtctagatgcattt ttcttcaagagcagtggcttgggatttactaattcagtgggtaaaccaatttcaccaaac attctggtcatgactaagagaactaactacatttaactaatggtggtgacctatagagat tttaggtattgtgaatgttttattatccccactccgtctgaaactactgtttcagttacg tttaatatttccttgtagccttgcatgtgaaatcctattttactgtgagttctggtcaga aaggcctgacacaagcagaaacatgtcttagatttgctgttgtttgtttttttttagggg tctttgagacagggtttctctgtgtagccttagctgtcctagaatttagctttgtagacc aggttggcctcacactcaagagatctacctgctcctgcctcccaagtactaggattaaag gcgtgtaccacgactgcctggctagaatttgttttataatttattcttggtgagacttgg gattaggtaaagaaacaattgtaaatacttctctccagaatattaatgtggatttgaagg aggttcttgttttagaataataatgtgtgaacactgcattgattctgatgttagaacagg caatataagtgttagcacatactactgtcagggttttatcatgtgcttcctggagactaa ggcacaagaagaacatgcagttacaagttcttaaacctgggctgtttttgaaggcagcat ccttagcaagtgtaattcttttgagcctcggttgtctagtgtttagttagaaatatttcc agtttgaaagttgtgcggttttgtcagtatcatttacattctcagcatgtccagttgtgg aagaaagagttcagtattttgaaaattgtagtgttaaaaatacattagctgtcagatttc atttttctaaagtctagctaagtctcattagatattggtagaaacataagttttgtacct gataaatattgacatcatgtacttctgctatgaaatagtagagcatttaaaaatgttaaa gttgaaaatttcaattaaaaaacaatagatactatatgtttgtgttttatataagtgttc tgattcatacaaatatttctctaaagtgttcagaattagtttgtaaagcaaaatgtgtat tcttgggagattattaacctttttgcatcaagtttcaagttctaggtgcttttttaaaaa atgtatgtaatgtgttgaaattattttaggaaaacttgaacatggtcaaatatcagcaaa gaacgttctctgtaatacacagactgaacgctggtatagctgtcttgtatgaggctatgt gccagcctggcaaatacagaagtggatgcccacagtcatctataggatggatatacacag ggcccccaatggagaagctagagaaagcacccgaggagctgaaggactctgcaaccctat aggtggaacaacaatatgaactaaccagtacccccagagcttgtgtctttagctgcatat atagcagaagatggcctagttggccatcactgggaagagaggccccttggtattgcaaac tttatatcccccagtacaggggaccgccagggccaagaagtgggagtgggtgggtagggg agcagggcgggggggagtatagggaactttcgggatagcatttgaaatttgaaatgtata taaagaaaatgtctaattaaaaaatacacagactgaaaaatgtaacatgaaatattagac catatgctagcacacagaaaaaaacattttcagtatgtaagttttgagaaataagttgaa agcctccataaatacccaatcttcataatgcttttaaaatgcaacagcatgtcttggcta caaattctagctcatggactaggttcttgtaaattcattaactagttaattaatttatgt atgtgggtatttttgtttctatatatgtctgtatatacctgacctgaagaaggtgtcaga tcctctgtaactggagttacagatggttgtgaacctccatacaggtgctaggaattgaac ctgtgtcctttggaagaacagccagtgctcttaactgctgagccatctctccagccccac tcatgggctcttgtatcttgtatactaaacatctggattttattctcattctccccaccc cctttccatcagtgagcctcttctgtataaagttccctttcctattttcagatccttagg ataggctaccacaagtggagttactgggttagagagtatggacttttgtgtgtttttacc tacaatatttagatttgttgttaattgatttatattcattgtagaagataaaaattacag cgattgtgcttcttatctgaaggtaaggttttatggttcaggaagcttgggaaattgcca ggataataaagctccttgaaggggacttatgccaacatccctgaaccttgctggtcactt tataggtggctttgttgttgttgtttgtatatgtttaatagttagtgaatgatttatata ggaattattttattggctaagttatatcttattgatccaagcaactaaaccaccaagctt ttagagatttatgagaaaaggtagttaaaagtctttagtatgattttttttgaaaaaaat tataacttacttaatttaaaacttatttattactaacgagcgcgtgaatctttaattata tacttagactataggcaaatgacacgaaaatagacagtaacaaagattgtacaaatcttg tttatcccccttttcctcctacagaatgtgaaaacctttgcatcttctgatagtctagcc aaggtccaagaagtagcaagctggcttttggaaatgaatcaggaactgctctctgtgggc agcaaaagacgacgaactggaggctctctgagagggaatgcttcctcaagccaggttgat gagggacagatgaatcgcgtggttgaggaggatccacagcagcaagcgagacatcaagag gaggagcacactgcgcggaatggtgaacttgtgggtgcaaaccctaggcctggagaccag aacgatacccagcaaggacaagtggaggaaaataataaccgctttatttcagtagatgag gactcttcgggaaatcaggaagagcaagaggaagatgaagagcatgctggggaacaggag gaggaagaggaggaagaggaagaggaggaggagatggaccaggagagtgatgattttgat ccgtctgatgacagtagcagagaagatgaacatacgcacaatagcaatgtcacaaactgc agtagtgtctcggacctgcccgctcaccagctctcctctccattctatacaaagacaaca aaagtgagtacctttagtccccttccagctggtatgctacctcgctagttcagttatagt gaagtcatccttgcaatcttcacatgcagaacaactttagtaagtcagttttagtttgtt aaaggctttagtgttagaaatacagatactaataagaagtcaacttaattgcaaaactag ggggtgcggattgatgttgtttaccaccaaaaaaaaaaaaatcaccagcttcacctttgc tgccagagtggtttgggtgagtgtgcagggttggtgtgaaatatgatgtgtgtaaattaa gtaatgaagaatcttatttgcagagtagctaatgtagtcactgtctgtcgtttcaccagc gttaactctaatgggtaagttagtggaagtcacagcaaatagttctgcgttgttgttcag aagttaaccgtggctcatggatgtctaagtagactagctttcagttaacctgttctaggg attgcatggagtgacaggtcacttgttctacacacataaaaacaaacagtccacatcaga catccctttactcatttgctgatttttaaatttaacaatgaatcattggatttagtctgt aaatacctgtaaatttaacagtatctctttcaatatggtttgactttaactatagtatct aatacagttggcttaaaattcaatttgctgaatcagttttgtttttaggttgtaaaacaa actgtctcagagaacgtttgtacctctaatctccagagcctggtcacgttgttttattta cttgctggcatggcttaggaaggaagtggcatcaggtcatggctctctcccatttaactt tctcaaaatggagggttttggtctgggctgattcttagtgttttgccgttctttttattt ccgtcatagcagtttagaaaatcctctagcactctggagggcagggcaggaagattatga attcaaggcaagacattctctcaattgcttaaaagacgttttaaaagttacctgtagctg tattaagacttcacatatcgaaatctattaataagaagaaatttatactaattcagtatt ttattttggaggcctttacatctttgatcattataaaacaacagaattgtacagtatcct aggctgcagtttcatttcagtccgcaacgtggggccttagagaagagtagggctgagatg ggaatcctactggagaggcaggtgtggcagcctcatcacttaaggcaggaagattgttgt agatttcaggctggcctgggctgtatagtgggtgtgagtctgggatggactgttcagaag acttttatctttgaaaggaagaaaacgtcttccacgttctcagtttgctttaaaaatgtc cctgtagaagtctccagcaatcctgaccgcagctccacttagtgtgtcattctgcagcat ttctgcattaatgagaaaaggcgtgtgttctctgttgcattggtttactctacagagcac aatggagactgtccagactaagggcagtttcctgcccttggtcattgagctttccttttt tgtgagtgtagagaagacggacagcagttttgacctaactgtgaagtgggcacccttaag tgctgagtataacagtggccagtggttcaaggcagacttgtactagttcttaaaatttct gaactcttgacaggttttttgaggtgctttctgtccaggtgactgacagacccatttgga ctaactcttagttatagggaacagtctctgctctttataagaaagccatgtttcctcttg gggccattaagttttgttcttaagggaatatgccataagcagtatagagtatattagata ctagataaacagatcgggaattctaatattaagtgtcaaggctaattgtagtcaataatt gctgcttattaaatacaaactgtttacacgttcctgaatagataagtaaacaggttatag ggagcccaatggttcactccacaccacactttctttctcatgtttagatttttttctatt tttcttttcttgagaatagtttttttttaaatacaatatattctgattatgcttcccccc ccaacttctccaagatccttctcacctccttcctgtctgtacaccccaccctttctgtca tgtaaagaaaaataatagaccatgataaaacaaatccaaatgaataagaatagacataac tggcaaacaaacagaagcaaaagagccaaagcatgagtaacacatacagatgtagagaca taaacgcttatacacataggagtccataaaaacaaagacctggaagccatagtatatatg catagggtctgtagggttaaaaagatgaagctttgacaaaacattgtgagacagagaacc tccatggatgccatgtagtttgtttgtgttgatcatctactgctgggcactggaccaacc cttaagagtggtttgtttcctcagtgagactctgttggaacaaagtaattatcaattgca aatagcttctaggtttgcaatggggctctgtctacttttcctctcagtgctgggacccca tctggcacagacctgtgcatgttctgagtaacaagactttctaagaaccctttctctttc cgtgtgctatgctgccccctatggtaggatgtaacacacagtacagcagagtttccaagt ttgataatgaaccccctgagaactcagctagctgcttttcttgtggtgttaacctgactt tattgaagatatgaggccacaagacctaagttgtaagaggaaggaaggatcaggaaaaaa ggaattggaggctctggagtctattctatgctagctgtttttctctgtgtgacaagtctt caccaagttctagagtcagtttgctttgccagttatgagtgtttgtctttgcaggtttaa ctgtgccagcttactatatctttttttttccctaaattattctctcaagagattctcaga tcatattgaatctttggcatgtgataatttaaaatacagattattaacgtttgcttctgc tttatctttatcagctcactttcagctaacaggaaaagcaaatggagctacagaggtcag gtctttatcctcttgatttttttcgtgaaaacaagcatgtttgagaagtcatttctgtag taactccattctttatctctaattattagattcttcctagtgatggtttttatccatttg cttctaggaagaactccatatacacaggcatacacatgaatacatatgcaaagtagatag agaatacattgtgtctttgagctctaaacagtagaggatgagcagggtctagttgcaagt gcctatagtcatggcgaccagagagaagggctttagaccataaacttaaagtaaacaggt ttgtgggggctctactggagctggaactaccagaaaactctatataataatgtgaccaac agctgaaacttaaagggaagctaggttatactctagagaatgatgttctcctcagaaagt ccatggcttagttctgtccataactctaaactcccaagttgtattaggtctaggtatgtc cagagagcagttgtcttatataacatttgctattatgcacagccctgtgagtggctgtgt cctttccttgtaaatgagaagaaatggaaacatggtgaggtctgcttaatttgattcaga atctgggggagatgatggcagggaaaaggcaggctgtgaaggatgaggtaggttccctaa acacggggaactgtagcttgtggtgcctgaacgtggactcttgactctttcactgactat acgaccatgtgtgatagatagccttattgaacttttctttatctgtacaatgattacctt tttttagaagtttttcaaggattaagtaaaatcaaatatatgaagtaggtagcatgatgc ctgagaaacgaggctagtgtgttcccataggccggtgacctggctttgtatctctccctt aaaatacaattcattctctataacacgtatatctttttgtgattttcagtgttcagtcat tatgtcactgctaacatacaaaagatatggagaaataagcagaaaataggttaatacttt aacaaaatgcatgcaagtagttgaacttaataaactgggcttcagtaaaaagaatataag ctctaatcaaagtgttgccagatcttgtcagtcatcaggctataagctattacttggagt agaatttgtcagtgcatctgtactttttccaaactttaactgtaaagtttctgcttacat gctctgtaatccttgcatttgattgataaagtcaatgtcaaattgtggtaaatttctaat taatttctcttaatacagtttttaaaatatttaacattgtttgaaactatatttgctcaa tatatatatgtgaatcagattatttttaatctatattccattcactgaaagtagtgaaaa gaatatcttaattaagagctttctctgtaaactaaagatattttccctatagaacatgtt accttagcacttgagaagcagaggcaggcatttctctaggccagccatagctacttgatg agaccgtgtctcaaaaacaaaaacagaatggggaattttaaaacttgagtacaaatgata tcattgagtacaaatgataaacttctaaaatatgtattagtaccaagcagagttattcca agatgtttttgaggcagatcgctttatatgtataattacaagggcagtctttttaatgtt attttgttggtggtaaggaagatggtatgtagaataagcatgcagtcttcaggatgaatt tcattttagttttgatatataacttcagtattcttaagtatcatagcaggcatttgttag tatcagaaaatatagatgtaactgctaggaaccacttattttacctaggcttttgtgtaa cttttctaatgcataatagagggttagagaaaaaggttttcttcccaagaactggcacat gcccttaaaggagggagagaagtgagactagaaagtgtcactcacttgtgtagtgggaag ggtgggtgctttgctgtctggccttgatcagattccacatctcagacacacttggagctc tggcaaattctagctaggtcagctgactccattgctttctgagtttggctagcatggggc tctatttcatttatgtttgtgcgtacttcatgcatgtgggtactttagaacttagaacag gtactagatcctctgaagctggagttaagacagcccttggtgcctggtggggattctggg aatagtactcaggatctttggcagaccagaaggtgctttaactcctgagccatttcttaa gctgaaaagttttttagtatgtagtttcctgaacaaattctttagtgtggccctgaaagc ttctggctctccatgtgtctgtcacatttctattgttgtaaagagatgccataaccaagg caacttatgaatgaaagcattcagttggggacttgcttacatcatggtagagagtgtggt ggcaggcagacagacaggcatagcactgagcaagtagctgagagctcacatctgaccctc aagcacaaaagactaggtctggtggtgtgtgcattgaaccctcaccccacccccacatcc cacgattaatttctctaacaagggcacacctcctaatctttctcaaacagttttataaaa caggaccaaacatacaaatatatgaactagtggggctcattctcgttccaagtattatac catgaaacataaataactttcattgagggagggtggaggggacacagtttgagaatcctg tttttaaaataattttgaggtgcccaatgctttagcaggaaggcaatagtactctccttt tccttccctgctcccgactctccatttatatagacctgttactataagttattctgtaat ggaaactgacaaggagactgggctcatcagctccaaatgtaaggtcatcactcagcttta tgtttaacagttctttatgttaacctctgtagtgagaattttggtttattaaaaaaaaat tcaattttgttgtgtgactatgttttttgttttaatcccaggtgtgggatcagaggctgc ttcacagcaggtgattatgatttgccttgtgcactaccaggggcgtgttttttttttttt tttttttaccagctacagatagtttaattctggggactctagagaaggtataaatgccag atccccaagaaggctgctgctccttctcctgctcctggttgatattgttgtagtttgaag agaagaagttggagaaatcttgctgatgaaaattggacttgccccccaaatccagcatcc ctagtcaccaggaagtagtctattttgttggctgaaagaggtcttcacccctctcccctc taaccttttctcctatatagtgttcagggttggaaggggttagggtggaataagggttgg aagagaggtagagataaaagaacccaataaaatagtaaaaaacaaaatagcaccaactga cctctttctggtcattgtgtttccatgtagagtactgacatctctgtagtcttcagtaga agactgctgttataaggaatgtatttggtgtattctctgaatcaacgtcctgtttttggt ttgtttgttttcgttgttgttttccttgtttttttttttttttttcttctggataagttg ctcttgaaatggcatgcccttttttcagcatgctaatgctgaggggcagttgaccacacc atcttttgtccttattctggtcctcagcagtgcagtggaagatggactacgtaatggtga tagccatagtaatgattgatatgtagccatgtgtgtggcacgttgcttttgtcccagcta ctttgtattttcttcccaaggatcatctgagctcctaagttcaataccaggctggggaac ataggaaaaaccctgtctcagttaaacaagcaaataaggataagtcatttattgaacaca tttctggggtaccaatagattccttatgtggatgtccttgctgagtctcgtcgttgccag cctcgtgtctgagatgacttatgagcaggaattcagttgtcctcaggttggtagcagaga tggtattgagctgagatttcacttcagcccaggttgttctccctgaaatacccagtgaag cagtatttccaactttgggatctgcagactatggaatgaccataaacctaacatctgctt tcaggtcctcagtgtcaccccactgtggcaagtttaaatacacagtttcatagcgcaagc ctgaggatcccctgggtccctgaattactgtgttggtgacatagttaatttgatagcctg tctaaacaattgattatttaaacaattggcctcttccttacccaactgaaattatgctca ttagcataaattcttcctacttactttaatctcatgtttcatcggtgcgctcaccctggc ccttcagtcctctaaccgtagaggaagaaatggcgtacactcaaaaggaagtctgtcctt gagttaacaactgattctccagacttgtgaaatggcttttttgggacagtggatggggtg gggctttcctacattagccattctttgtaacttttaaaaattaacaacctttttattttc aaggtaatagcaaaattgagtggtaacacagaggctcctgtatgcttcctgtctccatcc acacatatcataccccacaataagcccctcaccagaatggtagatgctttacaaatgata aggcagaggccacacttgcatcgggtttcactcctggtgttatgtattctgtgggtttga acaaatgtgtaatgatgtatttaccagtgtggtattatataaaatagtttccattgtcca ttgtcctagaaatcctcaagatcagcctattcagtctccatccacccaaccccaggctca ccagtctcttcactatttccacaacattcctatttctcaagtgccatataattggaatca tatagtttgtagtcctcttagcttgcctttttagtaccattaagtttctttagtgtattt tcatggcctgaaagttcactttttactgttgaataatattttgttgtctgtttatctatc catccacctaatggggaatattttggtttcctagacttcggcaaccgtgagctgcagaca aggagtagtgtagttgtatatttcctttcgaaaaggaaacctatttgccagggtgtcttc tcttgcctgtgctgtttttgcgttcttctcactgtcagcgactgctctgttcttcctggg gcttggcatcctcagggctattggcttgggctgcttgggctgtgttgctgtccactgtgg gcatgtgcacgtctccagtcgggtacatgcatatgtgcatcttcagactgttctcttttc ttttcttttcttttctttttccttttccttttccttttccttttctttttccttttcctt ttccttttccctttccttttccttttcctttttcctttttcctttccttttttctttcct ttttcctttcctttttcctttcctttttcctttcccttttcctttcccttttcctttccc ttttccttttcctttcccttttccttttccttttcctttcctttttcctttcctttttcc tttcctttttcctttcctttttcctttcctttttcctttcctttttcctttccttttttc tggctcttttcagtttattgcatgaaggagttacactagtccaagttaaaagcagacccc aaatgattacattatacaagctgtgaggtttttaaacttgtgacaagggacagaagggaa attctactcattgcaaggaaatcctcgcttaagcttcagagagccacaagcacttaaaac ccatgagccttcagctgatcgtccttagccagcccagtctctatcaggacctggcacatg ttcttgcgctggtcaccctgtagctgaattacttctccatattctggatgctcaattaca gtaccattgcaggcaaatttcttcttaaacaccttcactagtttctttttatcgtaatca tcagcgatcccttggacagtggtaagggtcttcctgccctttctctgttgagttcttata tggatataatcctcagagccagcaggatgcaggtcatcacccttacttacttgcatcagc aaaggggtcgaaagagtggaggttctggatagcagacatacagtacgattccttttcctc agtggaaatggcctgcgggagaaggcgggtggcggggcgggggggggggggggggcggag cgtcgggaagcgagggggctcgagggggaggcagctgagtccttggcggcggctcagtga ctggggctagactgttttcttagcaatgttgtttttcaagagttttatggattttttaaa aataaaagttatttgttaatattttacaagtatttttggcttgtttgtggtgtgtcttct tattctagtcatagtgtatttcagagaacctgcttaattttaatgatgtgcatcttctct attacttcattcatagacaatgcctttcatgttgtaactacagtcaaggttatctgctta ggtttttttcttgcgtgtttgcagtctgctttaagataatttttgaaaagagtgtaaggt ctctgagtgatgatgttgatgctgatgtagaggttcaggtgccgtggtaccatctacagg agactaacttttctctattatattttctttgtatctctgtcaaagatctgttgactgcat ttatgttggtccatttctgggtcctcagtcctgttgcgctatctgttccccaattgctct cttgatggctgtaactttgtgaaaagacttcaggataggttagtgtcaatctttccaggt tgttcagtattatatgggccctttgaagtctctaccgtaacatttaaatgttggagcatt tgttagtgtctgcagagtaactgctgggattttgtttggaacagtgttgagcatttttgc ttctgctctgccttcccgtgtttagaactttagaattcctttatctgccttcccgtgttt agaactttagaattcctttattatattttcagcatatggatagtaactcaaaagacagtg caataatgaaaacctacaaaagagataagaatgttcagttttaaagacgtctacaattaa tagatttagcagtgtccttagtaagttggagaaagtgatttttgcagtgaaacatgtaat cattaaagtttcttcattgaacactagagggccatatagtaccttgttaatggtgctgtt ttcatgtatttaaatatcttagtgtctgcctattatttcttgctccaatttggaataagt attaagggaagttgtttatattgacatataatatttttgtattttttaagtcaagaaact agcatatttcttattttgacacatagtaacaaaaatattcagtgcatcttgtacatgtgt ataaactcactctctcctcaaggatgagtacattttgaaaagtccttaagaattagcata taatcttcagtcagcagacctcccatgaccagtgattgccctgggcttcctcagcatatg tgcaaaaagagcaggtccctggcgttagcactacagtaggctgaggacgcctcgtcatct tgaagtaaagaatgcttgaccagttaattttcttgacttttcttagtttggtagtggggt tgaaccataatgtttaggccctaacaaagtagagtgactgtgtgaagaggaacaggaaca gtttgtttagacttttggaatatggatgactacttgtgaaagttcagagtctgatcagaa tcgtgctgaaattgtcaacttcttgcctctgtttatttgatagaatgcttctcattctta atttttgtattctgggggacgtaggacattctcactagtcagtgttcatgctgtttttaa gaactggtcaaggatggtgaacacagtgggcgtgccttagctacactgtgaacgtgggga agggatgggttggactctgagactgccaagggtaaaccaccacatccctgagcctctgca cctgagctgtcattattaagccacttttaggttgacaatttaaattttctgggcaggtta gtccctgaccatacatggttgttagagtcgatatggataaacagtggtgtctcgtggtgt ggttacggctggtgtgctctcaccacacactcactgctctgtaccgctgctgttcttctg cagaatacacacatgcttagttctggcagagtcatgtgagcagagcttaagtgactaaac accttttgctccaggaatgaaattagttacctcatctattatgttggtgaaagttaaatt cttaaatggctaataacgcaatccaaacgagaagagggatcggtatcccgtaaagccttt ctatggctctgttttccagagcgccaccttcatttacatcatacacagagaaaagtagct gttctcactcattgcaaaatcaaagcagttttcatccaacaaataggattaatagcagtt ggatagaaacatagcattgagtgccacaactgtattatatggtctgttttcattttcaat tagattattttcagatgttaaactgccaggtgatctaatttctttatcgacatgattatc cttgtttttattcttttgtgtgtgagtgttttgcctatggaccacatgtgtgcctagtgc ctgaagaggccagaagtcagtgtcatatttcctgaaactggaattgttgcttatcatgag gcatcatgtgtgtgttgggaattgagcccaggtcctctagaaggacaatcagtgctctta accactaagccatctctccagcccgcatgggatcccatttaaaggatgtatcccagcttg cacaaggaatctgaggtggtctgtatttcggagtagcatggtaaaatggagtatatgtga gaaccagcgcaatgggaataatgcagtagaagtgaggctgaggccagagaggacgaaagg aacgatctacagtgagttggggcaagctggtaatagcacgtaggccgggcatttctgtct tagggttttttactgctgtgaccagacaccatgaccaaggcaattcttttattattatta ttattattattattattattattattattattattttaaagatttatttattattatatg taagtacaccgtagcttacttcagacataccagaagagggcatcagacctcattactggt ggttgtgattcaccatgtggttgctgggatttgaactcaggacctctggaagaacagtca gtgagtgctcttacccactgagccatctcaccatccccaggctgggcattcctgaagttg aatcccagtattcttcagagtgtgaccctacaccacctcaaacatctggagtgctaatta ggaatgtaggtcggccaagtctacctcagaactacttagccaaactctagagctggagta tgcatttaagggagctgtcctaacattcgtagcacactggttgtccatagagtggtgcca taaaactgaggacagtgtacacagccctctcaatgaagaccaaatattctgtgtctgttc tctctgcatgtctttctctcttactgggtttcatgagtaccttagcagattgtacccttg gatgtataataagtgcattaaggaaacactgaactttacagttagatagctagtcaagac caaactgttaactctttgatgatattaaggatgctggtagtacctgtcacatagtatcac tgagagtgtttattgaagcctgaaaggtagccaggacgagtcctgagagtcttcagcagc tattagctgattgtatttcataatgtcaattatttcaaagttacaagtttgtgctaaagc tagcttgctgggatgtcatgcaaagcattgttggtttctaagaggtcatctggtgcattg aaactgttctgacttctacctaaatgattatggcatttaacttggagttgaactgtaggt tatttaagtttagttagtactaaccatgaagtactcattttgtaattaactgcttaatgc tgaaactagaaagagttggaacttaaaatattaataaactgggggccacttgggaatgct gagaagagatgaaaacctcggttgtcttcttgctatgctatggtgctgtggactcagtga ctgcagtaactggaaggaggatccattcgatactcctgcaggttctgtccctgctgcgtg cgtgctgcgtgcgtgctgcgtgcgtgctggagagaatgcccacatgttcacactcacttt ctttctttttacctgtctactactgtgtggtcagtggagtattcacgaagcaaagttgaa agtatgatgacagaaaatttaaaagtgagctaattttttaaatcttacattaaagtaact caggtgaaatccaagccatacattttctatagttttatattttattttgaaaatagccaa agtcttgctaaattggttcatttttagtagttatcaaagtgggaaagtgttaccaaataa tctccaccctcagaatagcaactaatctttccatcttggatcaccttttgcccttttcac cgtggttaaaaattgaaattaacactctttggaaagactacatagatgccatatggtagt gaggactgtgtaggtgccatttcacagtgaaggctttgtaggcactgtatcacagggaag ctgtatactcggagccctttatttgtatgtatactttgtcatcccccggcacattgaaca ctcagcctgtacaagtcagagtgcagcctgaggtgaagccgtgggtctgagtcagcggag gctggttagtcgccttcccatcatgcagtcttaggctttacccagattgctcttctacct gagctgccctgagctttattctatgactgcagatcacttggaaacagcacagcagtgtga aaagcaagctccacttttttttttttttttttaatgacagcctaaatggatgttccttta aacagaaatgtctgcccattttttttaagtgaagagaatgtagcgtacacccttcagagt aagcaatattagcgtcctactgcaaaaaggaatcttttcttaaagacagggatactatag tgatcatctttgaaaatgtatatgctgtaggatgttgtaatatctatggtctccttcctg cttcctttttcttacgtagaggctagagataaatagctggtgtcttaattactctccatc tcaggcctctcactgtacccgaggcttgctagtctggctagccagcaagctttggggacc ctttcttctccatttgggcactagcattgtagatgcttgagcccactgagcatgcaagtg taagcatgggagttgggtgttgaactcaaggtccccatgtttacatagcaagcatttacc cttatgtctctatctccacagcctctcactcacttcctcttaactctcaagatcttccta cacaagcacatttgggattaaagaagttgagttaatttatcttttttgatagctaattag tgttttatttattgaagaaatgaaaactcagacatctgctataaaattaatgttgaaatt tatgagactgtcagtttatctaggaatgaaagtctaaattattttctatagtttgattgt atgtttaaagtattttatttagagtaaatattttttgtacttaatttttttttattttta ttttaaaggtgtgtacgcacatatccttccctgcagggtccccagaagcacagagtagtg tgatttcctggcctgcaaacgatttgtaagacttttgcaggagaaatagctgaaaggttg atgagtgagttgtgctaaaacttgcttataaaagtaaagggcagactttaattgtttatt ttgaatactgtggaagatgaagttgttggaggctctttgtcgtcacatggggatggaatt cttgcatgattgaaatacctgtaattacaatgacatttttgccatagttgacttaaaggt atagctatattaatcaccagctgaattcttggtgagcagctgtgtaaggccagtttaaca gctggggtatcccggcataagcatgagagttaaacttagtcacgaagtaaaatttgttac agacacagaggactctatcctgggcactgctgagacacttagaaaacgtaatctgaaagt tggactttagtttataatcgcaaatctttctttaaatgtctgataaaagaatgagaagtt tgttttgaagtaaatagttcatagacacaaatattggattaaaattcaaagcttacatta gaaaaattaaatctatcatctatccactttgtatgttattgcctaggtcattatggtgga taggaaaagaaaataaaaatatatgattttttgtagtgttaattttttgaatttcatctt ttggtcaatttttaaaacttttcattacatctataccttctttccagttaaaatattttc attgtatacaactcaactacactacctaacacattgccacgattgcctgttttgaaagag tatccatgctgggcagtggtggcgcacccctttaatcccagcacttgggaggcagaggca ggcagatttctgagttggagcccagcctggtctacaaagtgagttccaggacagccaggg ctacacagagaaaccctgtctcgaaacaaaaaagaaaaaaaaatgaaaaccaaacaaaaa gaaacgtgtagaatatacactactggattgaaacagtgagttttgtttagatgtattcta ttttaaagtttctataaatcacatttatttacttatttatctatttatttatgaggggga gggacatatacatgaaggtcataggacaacctttaagagttggttctttgctaccatctg agtcttagggatcaaacccaggtcgtcaggcttggcaacaagcacctttttttttttttt tttttcattaaaccatgttgctggcccttggatatgttttgtaaagctaactttactaac tcgtgataaagaaggctcacaaatcgatagtccagacacgcacatgagactaagaaagct acagtatacactccagactgtaaagttttcaccgtcagtcttctgtcccatccttctctt tagggaatctgaagtgtgggataagcagccagcctaaagttccttgtttcatggtggcac tgaccctagaatttaaaattcacctgataatctatgtgctgtgcagttgcctgtcagctt tggataatttagcctagagcggtggttcccaactttactaatgctgggaccctttaaaat agttcctttaactatagtttaatatagttgtactgtggtaatcataaaattattcctgtt gctacttcacaactatcattttactactgttacagaacataatgtaaatttataatggtc ttaggcaacccctgtgggtttgtgaatttgtagctgctaggtctatttgtagaatgcccg tatacattcggtactgtttcagaatccaagctactgtctgcttatgtgcttagtgctttt gggttgttctttgtgcttagatatcgaaacttaacctaggaagttcaagaatacattgta gtattgctagtacactagtaatggtgggatgccctagaaactgtaacttgaaattgtaaa cttcctgacaagtactctgagcatttaaatgtgtcgaacttctctggaaatcattttgga ctggaccttagctcaccctccctcatttttaagacagggtctcactgtgtagttctggct atcctggaatacagtgtgtagaccagactggcctccaactcaaacagtccctcctgcttc tgtcttctatgtgccaccactgcttagcctggactttaaagtttataaacacagttcttc catttgatttcgttttatgaatactaaaggtataggggccacactgtatagttaaaattt aattttatgctgaattgttttttctatagttgaggtattattaatacaagaagacagttt attggtttgtgactgttccctgtttgtttcagtttctccatttttatgcataaatttctt aacgggcaatagtgtaagagtcctttctctttggcactaggctatgaaagaccgaaaatt gatttagcacaggttgacaatattctatatataatgttctatattaaaagaatagttatt ctgacttatttaactttcagtagtgctattaaaagtatctagaattttctgtttcaccaa gaacacagtggtgttaacaaaagtggctttaagttcacagaagcactgaataatactgag atccaatatttttcccattggaaaaaatgtgaaaacttatacaaaatcagtttcttaagg ctggtgcagtgtcactttgcatgtagaagctgaggcaagaggattcttggagggaagact gcttgaggcctcgaatttgaggctagtctggacactgtaaggaaactagcttactgtgct aatagttgaggagccatgcatactgtacacaatgcccttcatggagtgtggttttcttga gtagctaatcacgtgaaccaggctcttgcttaatgtccgtggttgtgaggctctacaccc actgtccagctcgtgcttcgttcatcagggctttcttggatatttgaagggtttgaaatt tttcacatttccattctagattgacaacatggctttagaataagagttccacaaagtaca gacacttggaagttagtagcttgcactgatcatcactgaagagctactttatatctttat ccttataaaggcttggctggacacttcatttagtgttaggtttgctggagttgcagtgct catattatccagttgagggcagctacttaccaatttaaaagagtttagaatgctaccact tggtgcttctgaaaatgcagtttctgatatacaacagtgctcactttctctcctgtgttt tttattgtcacaaataatcatgggaacatggaaagctcctttttccttcgtaataattgt ttagctctttaagaactgaaggagaaagccttatgttcacttaaagttcttcctgtccca gctcctttagatcccgtctttcttccccgcttacatcagacttcaaaagtcgcatagatt ggcttatctgggaacctggatcttaacagaacatacacataaacaaggcggctttacagg tctctgagacttttatgtgtaaatgtgagaacagacagtgtttattgtgctgtttaatta atcagtgggctgagtaaaaattaaattgcaactggcctgtttggtatgttttttatgcaa aataaattatattctcacagaatatacggctgtctttcatttttgtttttgtttaagcac agagatatcatttagtttaaatgatatcttccctatagttctgaaaggaattagaagtgc taattgttttcagagattatcactgaaataaattattttaagaataaagatctcgttctg tataaaattatattataatattattagcaaaatagatagtatttatatctggggatagat atattagcaaaaaggttattctaagaagacgggcagaatatgagtgagggttataattat gtgaacaaaatatcagtcttcctctagagagtaaagtgagtgtcaaaggacacacacaga ctgggaggtggtgacatttcttctggagaggctggtgggaccatagacatgtttctctta actatcagttattaccttcccccacccccccgcccctggccctggccataataatttata gatttccagtgtccttatgctgtcttttctctggcctacccctgggtctttgttgggaat gatgttgtgcatgcactgccatgcatctcttagagcatgtccttgtttagaagtgacatg tgactgtgatcatggtccttcataaattattttataagaaaataagtgtatgtgtgtatc tgaatgtgaatgtatgtggatgtctgtctgcatactcccatgcagagacaggaagacact gatgacttgtgcaatcaccccttgccttatttccttgagatacaagacagtctcttggag ctaagtagaggctaccaatccccagggattctcctgtctcagttccctgtacaacagtta ctgactcacgggaccacacccagctttttttgtgggtgtgtgcaaagcttcatactcagg tcctcaggtttgaacagcaagagccctgaagtccactgagccaagtctccagtcccagtg ttaaggactgttgttatattctactatcatttactttttaaacttgttactgtggttgag caggtataacataaaaagtaatgtttttccttgttcacgtatgttcagtggcattaagta cactcagaatattgcataacaattaccaccttccctctccagaactctcccatttttttc ccctgaaactctgtagcagttaaacatacttatctccacacccttgtgctttctgtctct gaactgaactgtccccacacactgcttaagtgtttgattataagtggagtcatgccacac ctgtctctttgtaatgcacttgttttacttagtatgatagcctcaactgtcaatgatgtg tcaaggctggaattttcttcttgaaggcaaaacagtattcaactctgtgtgtgtagcact tagcttacttagattatttccatgttgtgattgttgtgaataatgttgctacactcatgg tacttctagtcaagttcctgcttttaaatcgtgtgtatgtccatgtccatgtccagaagt ggaaatttggagtgaccccttgactgtgggcagtgtttggggagctgccataccacttcc catcgcagttacagtgttttccatcatattccagttcccctacatactgccagcaagttt gcactttaagctacatttcaaatttgatattagagagaaagacctggtttaagaacataa catccggatcgggctaggaataattcttgtgtacaaactgtcttttctgctcattagtct acttacgatagagtaacattgagctttccaaagtgggaggggctgggggaagaagccaga caaggtggctcaccctgtaattctagcacttgggagttagaggcaggagaatcaccagtt ccaggtcatttctggctgcaggatcgaggtcgacctgagtggttcgtttaaggaaatatt taagattacactgcaactaatagtttgagaatactttcaggtagagtcgttgcctctcaa gtttaccttgggcgttagggagcaatggaaagttctttaaacgtagatgttatagcccag tcctttgcgttttaggaagggttgtctaattgatatgttggaggggttaagcacagagaa tccccacctaccatccaacatttcagtcacctctgtgtcagagtactcggtgacagggac acactgccatgggtttcttagagcatgtttctgctgagaagtgacatcgtactgtgatca ctgcctatcatcgttcattttataaataataaacaatttatgtgccttcagtcagtcatg tggctattataatggactcaggaactgtttcagaactacagaggaactgagattgataat ttctagtaagataatttgaggttattaacagcgagagagcggtagggtatgtttgctgtc ttggtgagcaggagactggtctgtaaggtcagtcacttgatgcagaggaggctgaacaag caagctgttttcagcatgcatcaacactcctgaagtttgaatagactcaaagcattcttc agacacttatggtgacgtttgagaaaggaagacttaagagaacatagagttgtgtgtgtg ttgaattttgaaaaggaactcttactttccactttctagggagcaccttggttatttagt tggaacgctcccaggaccccgcagcccagccctgctttgctcctgtctgcactgctcact tgctgtgggagctcaccagctgtttgactggcaggagcaggagtgctcacttcaggagtt tataattcctggctattctaggacagtttgcacttcaccaagcctcagacaggtcaggac actcgcaaagagaaggttggaagacaaaaacggcaacaggccttggattctcagcctttt aaaactactagtcattttttcagtttggttgtgagtgttttagtaatgtgcctgtagtac atattgagagtgttcatcaaccaagaggagtttaaaatgtcaaaacctggaaaagctcct gtgaaccatggcttggttcctgttgatctcaaaagtgcaaaagaccctctgccacatcag actgtgatgaagatatttagcattagcattattgcccagggtctgcctttttgtcgaaga cgggtaagtctgtaacatttaaagcacgactgtttattaagtgctgtttctcttcctgta aagcactgcttccgatcgcctgtgtgttgtctgtgaggagagctaacgatgctttgtgtg gctagggaaaacttacacagcaagagccgggcaggggcaccctctctgcctttcgtctcc tctgtttttcatgagattagaatcctgtaaacttatttcaaactggtaaatgtgaaaacg gtatgtcttgaaatatgctgataaatgttagaaagttgtgtagcttttattttgctggga ggccaaactgctgttggtatgttccttacatgtgggaacgcagcatctagtgcaaagaac actccaggcaggtctgtaggcttcttgggcccgtaaagctgggctatttttacaaactct ttcatgaattaaaacttcatgggttagaagagatgtcagttgttttctgaaacagaagcg gcacatggttgggtgatatcccgaatgtcttggactttgcagcactcgtgttactgtata atcagcataaggtccaagggtgtttttcagtatgacgcactggggctgtagtattagcaa tatgtgtttcttctgacctagctgctgttgccattttgaattatttactagagtcatgta tttatacatatgaaatatgtttatggtcctgaacacagtaggcccaaacacataagtttg tttgtgtgtctatgtctgtctgtctgtttctttggaagtaaatgagaacttgtttaatca atgttttctgttcttacatctcttatagttactttaattttttaactgcaggggacaagg agaataatccacatcactagaaaacacttttgtctgtatagtggcatttactgagatata ggaaagctttgtaatttttttctaattagcttagttccttttgagtgatttcaacttcta tcttaataagtggcagccatgttggaaaatgaagttaaaacaatgtcttctaaattttga aacaagaacccaaagtgctctcttgtcctatttcttctcttcttccttttctatttgttc tgtcttcatggtttccttgtctttcacctgtcagtttgaaagctgctgtggagtgaggtc actgctcgcatttgagattttacaagaagcctctgacagctgtaatgtcattcgtaaaat aagcgattagtttactccttctgtaaaagaaaataccaacacaaataatttaaaagacaa aaattagcaaacataaaaacatattaaacaattgaagtattaatagcgaagttaataatg ttctcttctcttgttcttattttgtaaagaacatacggagttttagcttgcaggagactt tatgtcacagggcgtttgtttggaaactgcacagaagctgatcctgaaggacccattgcc gaggaccttgagggcttcctgcaggttttctcatgtcactgctctgacattgcttaggtt acggccgtgcagctgcgtgccaagataaaactatgtatgggaataaaagttgtactagaa aattagaaataaagaacatggctaacgatttgaaacagtcttaatcagaatgtccaggct ttgttttataaaaacagtgttgagtgtggcatgactcattgcctctgactcctggaaacc tcagagttaggagaccttctctttccacatttggttactttaaatggattttcattatct ttgttagtttagatattttaagtgtttatgagagaactcttctggagtaccatatagttt gctcttattttatgccagatgtcttggagagcatcatatcccttccagaggtgctgactc ggctgctatttgtggggagctattgttttgaacaaaatcgaaagcatgacctatgaaaat tgtgggaaattctttaaaactataattatagctatattttttaactttcatctctctctc tctttaaaagaaaatgtgtttatcttatcaaagtatcataactatgagggtagtctaatt tatcagtttttctaggctctcacctaatgtgtgtgaagattaaagtttttcgtaacagta aataacaaataataaactagctgctaatcagtcactattgagtactttagcattaaggaa cccatcagctagaatatttggtaaaaatcagttccttagggggccggagccatagctcca ttgttaatagtgcagactgctctcataatagtggccaaaagtttggtccccaggcgtcag atggtgcacagtctacctgtagttcatggcccactggcaccctctactggacttcataag aacccacattcacattcagagactccctgtacacacacacacacacacacacacacacac acacacacacacacacctctacctgcaaaagtaatcttaaaaaataaactccataggtaa ttcttaatataaagaagatttggggctttaacttataattttaagaagctctgattatcc tttaggagaacagaaatgatgtacgagttgctttattttcccttgaaactggaatgcttt taattatggtagtaccatcagtaacattctaaaattccagtttcattatttttctacatt ttggtcacatctaaaataagaatgcagaattgatttttctttttagcaagagaaatattg gtgaatcttattgttagtatattagaagaaaaccattcttacagctaatattagaatgta taaatctcacttattcactcattccagctttggaacactcaagtatctagactgttcctg tagaatttctcttatgtgttactcttttgataggcagttgttggtttagatcagattctt aaacatctggtgtgctcattcagcgtggaagactaagtggggtagggcttgggcgcaggc agtgagtgtgggaaaacactcacaggtccttctctgttgttcattgtgtttatgttttga gacagtcttactggacagctcaggctggccttgaactagaaattcttctgctgtaccctt tctagtagcaggctttggttgtgtgtatcaccatgtttgactttaaagagcccagtaagt agcctaggaactgtgttactgggtgactactatcatgagccatttcggtccttattgact taagtgtgagctgaatgctgtagaccagggactcaaacaggtgagtctcccagtttttgt ggtgatcctgagcagtgatcttacctcctggcattccactatctctgtggtctatctaca tacaccttttcctaacatgcttggcacttttcagctgctgtgataattaggtttggaaga taagtctgggagcatattacagtccatttgtaatatgcttaaaatgtcttgtataatcac cttcaagatttttaacttaagctacacattgagacagttttactcattgaaattttgaaa tgtttatgtcagtggttggattgttctctgttgccttgttagccagggatcacacctagt cagctgagttcacctggcagccattgagacaggcagggctccctcacgttggcgcactgt ccttcctgtccacggcctcagtcagtgctttggacaaggacaaagtgacacagcacctct tcagcaacattgctttggtttaggatttgttcttaatcactgtgccattaatagagacaa agctgtattaaaatttttagtgttttttcaaatataacctaatgggctgttggttttgta tgtatagacttcagttagtttaggttgtatataattttaatcttgttcttttagtggact ttagaccagccagttaatttttcagaaaaggcctgccataattattaagaaaatgtattg ggtttttgtttgtttatttttttgttttgttttgtttttaggaagaatgtctaatgatcc cgtttatagtaaaattttcatatgtatagagattttttttgtgtgtgttactaaaaactg taccacaatcgtgaaggcctttcttggtaaagcaaaagtatgaattctgaatttagaaat gtgatatctccccaaattatctggattgagtagcttatgtagtaaattttattttcattg aacaacttacatctcaaaaataggacatgcttattgtatgcaaaataaaatactttcttt ataacttcctaaattattagtaattttactgctcagaaacaaccaaaacaataattttaa atatttaattatttttacgcaaaattggcagttagacccattcttactgtattgcttgtg tttctccaagactgtgagtactggtaagtgtctgatctgccacgtgaatgtggcagtatt cttcttctttgctaatttgaatagtgacatgctttggccatgtgtatacaaaaaattttt agtcatattattatgcacagatttgtagatgtaggattacttggtaaagaattaacttta ttctgttcttggtaggtagtaatgaattgttttaccgaaaattgtaccaattttaaaata ccacagggcccatctaaccttactgtaaaacatatatgtctgtttattcctatgtaaaca ttttagggacattacacttaggtgatgtcagttccaatatttccattaagtacatgtcat catactccatgaagaaagcataagaagaaatctaaaattatttgttcatagtgatgatct ttgttgtggaaaaggacctttgccagagacttgtcacctgtattatttccaactgtaagg attttctactcttttcatagctatgtttctgtgtctttgtccaaatagattgtccacatg tgtgattttaaaaatgtatttctgattcaacattgtctgtttattgctggtcttctgata gatgtcttggtttttatgtcatattatactcttacactcctggattttagtgagatattt ttatagtattaatggtggcctctcccaaagtgtactgctattaataatctccactacgag agtccttctcctgcgagctgcagcagctgccggagacacttgcctgcttcttacccagtg ttacttaggtcacatctgccatagctttgcccattagccactttctaggcttacccttcc tccctctctctgctgagttccatttccaccatgattaacttgactatcttagtttcagca gttttcttgtttaaggtggccaactgcaattcaacttggtttctacacagttcagtgtct gtttccagaatgttcagctgaatatataaacacacacgagtatctcttcagcagagataa atataaaacattttaacacacatcatcccccctgtgttacgcatggatttttgtccccag gttctttcaagacctcacggtggagcctggctggctggctgcaccttcttccaggcgctg tgcttctttgtgccctagtggtcctcgtccattatccttaactccagtgcactcgctgtt ctaccattgcccgtggcctggtttgggctgctcagtggtgctgtgtgacactttgtagaa acacttggtggcttcagttgggctatccactcagaccacaggggctgactccaatacttt ttatgctgcaggggatacgcaggagaaaggtagtgtacagatttgtaggactggttttgt tgaaaaagctgttaaagttggagtctcttctaggagttttcgtactcttaggagcatggc atgtttttgtttactgtgttttcaaatgactatgcagctgtgattttgaatatttcattt tctaaatttaaccaaactcagatatagtttgacacttaattttaaaagtaattactactt accaattttgaaagtaaaggctaaaacaaaaactcagtgtagtcctttaggaggtacttt taagagtcatttaggaggcatgagggcttgaaaatgccagaaaaggctaaaagagacaaa gacctaatactcggaagacgaaggtcagtattgtggtaggagctgatctagaaacagcat tgagtgaggatatagaaacattgtcttttgcaagttgcttaccacgtaatatgcaattta aagtcctaccagtctttttcttcctgagtactttgtacagacatgagtgaagagagtaga aatcaggagtttcctggggaaactacagtctctaacatgcaaggcactggctaaaaatag aagctcctatacaggtctatacaaaaatttaaagcaaacaagcaatcaaacaaaacccta caaccttttaagcatcttccgtctgtggtaaaacttaaactcattgtaatacaaaagggg aaatgcctaagaggcccactgtacaaagaggccaacagttaagaaagggcttcaggacga agacatcccgagttttgcaggcaaatagatggaactaggaaatatcatcctgagtgaagt aactcagacccaaaaaggcatgcatggtatgtactcatcagtgatattagccaaaaaaag tgcagaatatccaggatacagtccacagaacacaaaaaggttaacaagccaaagggccca agtgaggacgcctcaatcccacttgagagagagaagaaagcaatcatgggggtggggggg aaagggagggacatgggtgaaaatggggacagggagaggaagaagggaacatgaccaagt attgggtgggggaaaaggattgaagccctgagaaccaacagaaagaatggaaacaggcaa ccttgggtggtaggagatgggggtgggggtgggggactctccagaatataccagggacct gggaggtgaagagactctcaggactcaaaggggagggaccttagatgaaataccctagag tggggagagggaacttatagagcccacctccagcagaaagatagacatcaagtgagggat ggagttaccatccaactgtttaaaactctgacccataattgttcctgtctgaaagaactg cagggacaaaaatggagaagaccctgaggaaaaggtccagtggcaggcccaaagtggggt ccagctcaaggggaggtcccaaggcctgatactattactgaggctatggagtgctcacaa aaaggggcctatcatgaccacattctaaaagactcaacaagtagctgaaagagtgagatg tagatatttacacccaaccaatggacagaagctgctggcccccgtggttgaattagggaa aaggtggaagaagttgaggaggagggtgaccttataggaagaccagcagtctcaattaac ctggacccctgagatctctcagacactggaccaccaaccaggcagcatacaccagctgat atgaggcccccaacacatatacagcagaggactgccgggtctggactcagtcagagaaga tgcacctaacccttaagagactagaggccccagggagttggggaggcctggtggagtggt ggtggttggggacattctgtggagatggggggaggaggtactggatgtggaacagtcgga gggtggaccagaagaggggtgggagagggagagagagggagagggaggattaaataaaag taaaaacaaaaagaacttgagatatctttgtttttgaccagctgatctgagaatggcaag gcatgtggactgttgcttgtgtttacatcttgttactttctgttagttgtcctgctgccg atatccacctacattgtgccaataaacacaatacccaaaaccaaactgagagtttttctc taacctttggattaaacaggactagagagaattctgtgttcttctggagcccacagatta aatactcctacagatgctcctgttttgatgacagtaaagtgaggcagggagaacatctaa tttggtataaggataagcagatgggatactactttataagtatttctcaaaaataccctc gtatagtacgtaagatgacctcatgaggtatactgagttatagtattcctactcacttgt atgagagatctgaggcctaggagggtttaagaacttgtcttaaaactagaagataagtaa ttacttttgaatgaggtcgtcaggccgtgggactagtatgttagtaacctaatcacagta gagtggattttcattgcactgctgctagtatcactttgatcttgcctttggtgtgatgcc actaatggccactcaaagaccctgagtccatggaacagagttgagagccttgaatagacc tcttcataaaacctgtgtccttaacgttcttcacataggatgatctcggtatgtaattat gacattgctttctggacctttttgtcatcagatcttagtagtattgggttttgcttcctt tgttaatctagacataaagtaaattaaatgagcacgtgctatacaaatcacactgaggaa acactgagtcatgtcttattacccacaataagctggacagatgataagtagccttaatac caacaggcagggtactcacacctgcaacttgcattttaagaaatgagttgaatgtcaaga ctggtagagtgaacaacaaataaaaaaatactctaaaaataaacttggaagtaagttcct ggcagagtaaattgtttgaaataagtctgcacggccccttgcctgcacaccagaaatcta acaagtcctagaaactaagacttaaaagacttaaagttgacgtatgatggcctcctttaa tacttatctaagtgattattttgctgcagaaataatgtaatgtgctagaagtttttgtaa tatgtagtttatgtgccgtattatctttctgaagtgcacatcaaaatgcacataagtgca ttatctttcaaaatgcgcatgcatgaatgcagctgtcttcagagttagagaaccaagcct gttgaactgtaacattcataactatcatttatatgtaaaacttgaagactcttggagatt tcaggacggtttgaactctctcttaagtctctgtgtatgtatgaatttaaagcatacatt gcatggataaacacctaaatctattacctagttacagataggtcaatgtagtataatgac agtttgcagttaaaatattttcatcagtgattaaaatatggcagatatatacattaaatg atgggaaggaaccccagccacttttgacggtctgcacattaaaataagagcaaagctaaa ttaagtttaaccttccattacagattatgtctgttatctgcattttgctagtattgtaga tggataagacagagtggcctcccataaggaactactgatggcctttctgtctcttgagag atgtatctcctgttatcgttctatagtcatatgtcaaaaacaccagaaggacaagctgga tactatgtactgatgcaacacttggaattaagattggaagatatatgatctcaactcaac ttgggctgcctagtgggaccctgtctcaaaaacaaaaccataggtacatgatttcattca ttcatcagataggtattaaataagcaagataaccattatagttacaggatgcatttgtat ttgggttgtgccaaaatacaatgtgtcagcagacactttgtgtgtgtgtgtgtgtgtgtg tgtgtgtgtgtgtgtgtgtgtgtgtgtgtacatgtatacagggctccacagaggccagag atattggatcacctagaagtggagatccaagttgttgtaagccacctaatgagtgcttag agccaaactcaggtcctccacaaacccagcacacactcttagctgcagagccatctccca gccctcttttattatacacttgtattttatgttaaagtttcttcacaagtatatacacat acatggacagacacactttgaattgttgtagcccttagtaaaatatataatctgttctca gaaattctagtacagaaaatacagtttgaattctattttaaaaaaatatacatgattttc ctctactcatgtttctacagcaagactttttttgttagagagtctttagtgtcctgtcag tcacatcccagtggtttgcctctgagtcatctctgggtcagaatcaagtcctctgctctc cctctccctgactgtgcgaaagttgcctaacttactgtccccagcacactggccagttaa ataataatcaagtaatccaggagtgttgaaatgtaggatacaattttctactgagataat agcaatcacctatctgagataaatggatataagccaaaagaaacccaagacagaaaaggt taaatactgatcctaaaaggacatggacagttagttttgggttgtgatatctttatatga tggtactcagttctgttttgagaagaaaaaaaatatagaaatgttgaataacactgttac cttaaatttcttccttacaggtcttgagtgtggaaattgaaatttgttattcctgtgctt atttacgtttttgtgtgtgggtggttcataataatagtttatttgtgagcaatttaagcc caaacagcttttcaatcatccacagaaatctttaccagtttagatgtaaaatgtcattgt tactactacagtgaacatataggggcagtattgcaccgtttttgtaaatcttgtctgaat ttgaggatttttaaacagttgcagttgcagccttgttcttggacaaatgataacaatcta tctgacttttaaatgcatggttgcacctgctttgtgaagtccaattcaggttcttatgtg gattatgcaaaactcttgttttacagagtccttaatttgtatttgaagtcaaggaaatgt taatttaaagggtaaaattcatctgtagttagacaacaaaatgtaactaattcatgaaaa attgcaaactattaaaatcagtacagtttgtttttaactgctttataagaacttttcaag aatgtgagaggtttttttgtcaacttgtcaagcaaacaaaacctagaataatatatagtc ttactttaagattcttcaattgataaatactatttttcaatatttaagtagtgagattat tacaaactttgttatagatttttgcaggttttttttttttctgacttcagtgagagagaa ctgagcagttgtttcccatgtatttgttgttctcgatgctttcttcctgtgatctgagga ctacttgacacactgagaatcaacagtgaacaaagcaaacccaggcttcactgttggctc atgtcagaagagcagagagattaagtatgtggatggtggtgctgtccaaacctgggcgtg agctcttactctttcctttactaagtgagccttagtaaatcagctgagtaaatggagaga ttactactatctcatagggttgctgtggtggcaacgaaagagtccataggaagtgtgtgt ggctctaacaagagctcacgtcctcaagcccatgtggctaaagagcgagatgagttctca ataccagtatatcttacttttacttctgatatagtaataattcttactatttttagaatt attgttttgaatattttttaaattgtgagagaaaatgcatagcatccattttgaatagtc agttattttttagtttcctgaatgactgataatccctgcctatagaaagttaatgtggct tggttcgactcacattatgtagattcattcagtgtgactaccctgtgtaaagattaaccc cattatgtctatatcgatgagaaaatgataggtgacatctctgtattttgtttgcagatt cccacatgtttagaataagtgtgttggcttagaatcccattaagtagaatttgtacaagt actaaatgagaattgaaaagagtttattggttcatgtgttctgtcgtttgcttcactgtc tctagtcaatcccttccatttatgattttaaattctgaaaccctttctcttttttcagcc ccttaactttggtaattaagaagtggaacacagttgatgacatctttgaaaatgttctga tcaaagcggtcctcattactgtgtagagagagacactaaagcatttgaagaactcagctt tctcatctgttagcaaatatacttaaacatttaggccgttctttgctataccttgctttc ttcattcttttacattttagacgtataggaaccatgctttaacatgcttataggtatgaa gccctgcacacacagggcgaagcattgttcaggtctgcaccctgtttaatagcttatcag ctatgttcctgctgtgcctgctccccggaggggaagggttgttctgcaagctttcatatt agctttgttaccatgcagtatttctcagctgctcacgctcattaccatacagctggtgtg gagacgaggaggaagcaggcagaagttgtactggctacaagaataagaagctcttttgca gatggggtgctggcagatggggcatgtgtttgagcaggttttactaatgacagtgcgcgt taaatatcattctgaggaagtccaaggtcattgtcatgaatttagagctttcgaggttgt gcagctttgtgcagaacagaccttttcagagccctaattaataaaaatgtaaccagtggc aagcaacaactagaaacatctaatgagtttttctttattttagtatattttatctttctc tgacatgatctaagaagcattaatcttatcaattagtctctcttattactttatgatggc ataattttaaagttattaaaaatttcaagctatatacaaaagtatatgctttgaaaaaat acagtagcactcagttttgctatataatgttaaactattagaagatcaaatggcagaaga attggaaaactgtgtctggtgttgagcattctgaagattaagttgaagtgtgtgacacat cattgtaaagttagacagaaatggtatggacaggggtgtgtcttttgttaaaatattttg tgcttaattttttatacccataaaaacatatgaggttgagttgaaataactgccaacact tgctttttaaaaagttttagccagatgagggagcacacaactgtagcatcaggacttggg aggctgagacagtttgctactactctgtgctgcttggtgagtaccagcctagccaagact gtgtaccacgactgaagaaaaataacaagaaaaaattcttcagtggcttctcactccttt tgacccttttaattaactgtagttttttatatatcatattacaagatgattaaaaagaac agctaaagcaaaatacagctgtgcagtcgtatgtaaaatgtacagtatcccctttaattt taatgtaaatagaactcttttattcccccaccccccacccctcttgatccagcatccact cgctctgggccctgcttgctccgctccggctcaatctggtccatgggttttgggtttttt tttggttgattttttgttgttgttgttgttttttttttttttttttttttttctcattac agatggctgtgagccaccatgtggatgctgggatttgaactcgtgatctccaggagagca gtcagtgctcttaacctctgagccacctcaccagcccacaaatagaactcttattttgtt aatggagattaaataatataaacacacattacatataaaatatacatagctaatttcagg attcatggtcagagcatgtttgcttacaaactttaatgcttctattttgtgttttgtttt tttttattttcttgattagtagaggcacaaaattctgttaatgcctgtaaataaacaaat aaacaagcctgtatgagattttcaggaaaggttatactgttaaatactttaacagttata ctttttttcctttaaacactgttagagtatgttctgtttattgtgaaggtttgttttcag ttggctttttaaagttccagggagttttcagccattccattgcatctcatcacataccga gatgtatttctgagacacataactattctctagtgagaaaatacataaaagataaaatta taaataacatagaatacatataaatagccaacactttaaaattatttctgtaggtctgta tttatttggtagagtgtatatatatatatatatatatatagagagagagagagagggaga gagagagagagagagagagagagtatatatactctacaaaatatatataatttatatata tatatatatatatataatatatatatatatttgtagagtatatcttacattgccaatagt ctcctgtcaatattttttgttgacatgctatttgtacaacaagtttgggaagaactaaga taacacaagcatcaactgtagtcactgctgcccatgtaacttaacattctcattgggagg acagacaaacataatgtgttacagtgtacctaggttccagcttgactgagtgacagcttt ggaatgggagacataaccagtatgcagatgacttaaaccatctcttcttcaacccagacc ttctgagcttcagagccagttggtacatggtacatctgtctttggatatcttggaatcct gtcatgttcctcctcctgtcttctagaaagcagaaaacaatcaaacagcagcaacaaaac aatccttgttatgtcgtttatcttctgtctggtcaaacaaataagaaatttgatgccatc tttgatactgccctctcctttgtttctatcatctgtcaccagtatgcccagtcagttttg tgtccaaacacctcaattgttgatccctgtgtatctcttgctacccagctagtccaagct gccattgcttcttgcttgtccagtgacaaatccttcttggagcctgcccatatcctcttc cagtcactttacctgctttcaaccttgcatttaaaatcattttttaggaaagtttgtctg attacatcttgctttacagaagtccaatattcattggattttatgttattaaacataact tttcattgcttttatgttaaaataaaaattatttacaaagttttccataatctagtcctt ccttatttttctacccttattctgtgccactagtcatgcaggctgttccctgagcagtct ctgtacccccggactagatggaaccttaacatacacattcttcaaacactacagcctact gcatacagcatccccctccctttctctatctcctctcctttctttttgctttgttgtctt tctcaccctcctacagtataatctcaagccatgaaagccagtatttaattagctaataca gagtaggtaataaaattaatgcatactgaatgaatgagaatgtttgttggaaattaaaat agccatataagtctattggtaaaaattatacagcctagcaggatggaggtagagacagag aggataatgaagtattggtttaacttacagaggcgcatatttactaacaatgatatttgc tattattagaatgggacaaatgtgcctgggtcagattgacatctgttaaatgtggtttgg ctcctagaaaaaggatttatgtgataaaatcaagaaacagggccagatccaggtgtgatg gtgtctagtactggagagagagcagggcagaggtagaaggatcctgagttccaagccagc ctggactgcatagcatggtccaggtcaacttgggctgcatagtgaagccttggaggtggg tagttgcagaggagggaagcaaacacaaagaggtagccatattatatactgtgcttgtac ttaaatgtcctatgtgagtccatagaggaaatcctgtgactctggatatgctacttatat tgttagattgtatctgaaactgaaaatatttagtatcagatgaagctacaatctccaaga gtatttggattggtggagtcaatgaatagcacttaaagctgttttctttggttcagtagt agggtttgtcacttccatcagttgctcttgggatccagatccttgtagtaaggatgataa aagccttgcagcttccttgaatgggtgtttcattagagatcttcagtgtgtcctggtccc tactatccttcagtatttccatagaaaataaagtctaggaccatgaaggaatgagggtag aacctaagttcttcaaagggctggagaaccagctcagcaggtaagagcacctgctgctct tgcagagggcctgggttcagtgtttagcaacctcgtctggtggctcacaaccacctgtaa ctgcagactaggggctttgacaccttcttgtggcatccacagatgcttgcgtactcatga tgcacattggaaatgagcaggggcacacacacacacacacacacacacacacacacacac acacggaaaatagataacattttgaaaaatttaaagttcttagagggattagaaaaagga agcaaacttaatgtgttatgatcaattttatattttcttcctctgcccactagtaactgc tgagtcttaatatatgtaataccaaggataaaggaagttgttcttagcaaggggaaatta gcagttgtgtatcaactcttgcttcttttggtaaattcatacacgcagttcatcagtgaa cacatctggctccctggtagctagtttgtcttttctctgtgtctgccttggctttggtgc agattccatccacatatgatacctttgcatgctgacacagctcagaaaacatcctagaat atccgaaataccaatatgtcttacctcaatttagagaactctattttattctataattgg aggccaagatctggttctactaccatgtttttctggccctgggaaaattagtgaactctt ggcctctttcctcatataaaatagaggttatcatcatagcactgtcttcataaggctgtt gtacagttttcatgacccaatgaacaaaagcagctggaagaggcatgacttggtgagttc tcttcccagtgtataccattttcctaaaagggcatctttgggtaaatgaattgcttattc agactcaaaaagaataacatacatatatagcctctttcattgtggctactgaagggacac tcaatcatattaaaatctagtgactattttccctttattgttttagagtaaaatattatc acatgcctaactgcattatcatcatatgagctgaggaatattagttattaattatgccca tttgtcttctctgattgaccagcctactcttctttgagaatgtgggaggtcttctaggat agagacagctccatcttgatcagcttgcatcttgttttgcttaatgtctagatagctaga attttgctgactttggctcaatactttctcaaatgtggtagatgtttaaaaaccaaaaaa cccttaattcctaattaattttctaccttttccttcatcgtttagaacataagaacccat tgtatgactgagaatgttaggctgaagttgatcctattgcagtagagtttacggatgaag gatcactctgtaaatgattcctagttatcaccgtttcctgtgctacctgtgtgtaaacag tttagtagatccataaaatacgagttttcaaatgagtagagtagtaaggtaaattataac ttggcctggcaaaatctgtttgttgcaattgtttaataaaaactaggagagtgaaaatgt tttagaaagaaccatttaatttcatctttgctgatggtgatcatgttcattatcaagtca tcatctattttgaaatatctttaagattttttaatgtgctattttctctctataaaataa tttttaaaggaaaggcttaccatgaagaagaaattatttatctgtgagtgtatgtcttta tcttaagacctgacagatacctaaataaattattttaagattatattgaagttaattatc tttttgatagttttaagtttgaattttagctacatctaatatgtgattattcttcttgta aaattcacacgtagtgacttgcaaacttaaatttgtgaaaatttagaatttagttttaaa atgttctagcgtccttcttgtccagatccagagcaggtctcagctcctccacagcatctg cccctagtctgcccttccgttctacgccttcgtgtggaaaagtcaataccagtgcagaaa aataatcctttgaagttaggtatctaaacaacatttaaagttaatttttagacatttggg gtccccaaggtcttactgttagatatctttagagcatgtcttatttgttttaaagtgaat aaacaaaaggactataacaaaagattatgtgtaactctactaatggcatgtctgtttcca gcaatatgcttttgtattcaaattgaggagattctaaaatctcctgaataagcaggggat cttgtaaacattatgctgctcataattcttgcaatttgttaaattatacacactcagtaa ctgctttaaatgtaaagtggtgcttttgatatgactatttagcacctttctttttaaaca taatggggtgatcactgggttcttccgtgctcacttggacattacattagatgcttgaat acttaaaagagcaagtaagaactctgatgtacattcataaagagtgtggtgttttgttta ttagcgtacaaccaatgcagagtaggtattcaagcgaagtaagtttatattcccagtcat tataatgattagagtttcaaaaatatttttaacaggtcagttagggctgtgtagtgtgac tctgtctcaaaaaaaaaaaaaggaaattgaataaaattatttgttattttaaattttata taattactgaattggtgaaaaagaaatgccagtttctaaaagtttagtgtttgaatctga ctttatgtatctaccatccaaatattgtcacctgaatacagttgtcaaagtcagttgagc aacacatagcaagtcagagagttctagggctgatctgatgactccatgtcaaccaggcca gagcaagtgcacttttgttgaaggtctcctttaacttggaggctctgcagttatgctctg tgttcttttatggtcacagagaatagttcacgatgaaatgtcctttcagctccaataact gaaactaagtgcatcttttattgtagtagacatttgctaaaacaaatactggcaatacca taatagtcattggtcatctgagcccagtcgctcttttaaaaagtaaattatcaaatgcag aagtcagatgagaatgtagaaaacagaagagcagtgagataagggctgtaatggtggacc ggaagtgagttagcagaacctcaaatggtgctttacaagtggtgcacagcatttagggat tgggggttacctgctcttcagttctcttcttggtccaagtttagtggtctttgaaatagg gggttgattgagagctggtttcagggtgtggtaaatcatgacaggatgaaggggtaaata atatatatgttgaaaacaaggcaaatactgtttatactttttaccatgtctcttcatgtg ggtatgtgcacatgtgcctgcagatgaatagaggccagaagtcaacattgggcatagtgc cttatagctttccatcttaaattttgagactcagtctctcacttagtctgatgctgattt ggctaggcactctgggcagcaagccctggggtcttcctctcttcaccctcttcccccaac actagagttacagataggcacagtctactttgcctgactcctatgtaggtgttggtgatc caatctcaggccaatgcttgcatagcagagacctaattgtatctcttatttcaaaaagta atgattgctttgtgaatgatcttcaactttacaatgctgagaatacacggaagaatatgg gcagacagtatacagggaaattgtgttaatcattccattttagaagccttttaatgattc acaactttcaaactcttgatagtggtctgcaagatccttagtgttctgatcttgaccttt taccattgcttcagcctagacaaacttgtcctatccttgctgaacctgaacctgggctga aaagttgtctcctaggtgctaccattccagattcctctatccttgtaactgctcacttgt ttcatagcaccccgctctgtgtcttagcgctgataggagcttcctgtgtttgtttcctag tacactgctcaggcatacacaaatgagtgctcaaaatgcatcaagcttgtttatttattg acaggtgaggaaagaaggggaaagcttacagagcaatttagactgccaccattaacattt ttatatttaggtttttcttctctcttcctaaaggggccattattgaacttcaaagtagtc ctcctccagtttgatatgtaatgaacagtagtgtggtacgtgttttagaaagaaaggttt ggaggtgtaaacgggagagaggcatggggttaagggctgatggtagaacaggatgaaaaa attcaaaacaaactgagtttatgaatagtaagggtcctgttagtggaaatcaattagtca tgttccccctctgcttccctgagatacattgaggagtataacattttaataatggttggg aaatttctctggactgttcccccactactgtgttcaagagcacaacttgcttgtatgtat cagttctggtatgagtgtatgttgtaacaggaagacctcttactagtcattactaaagtg aaacaaacaaacaaaaaaaccaaagcagtcctcatgcctacttatacattcctgtaagtt ctccttgtatagaaaggcttgtgggattctcccatgaattaagatagattggctaataca ttaaagaacactgtaacttccacattaggtaattgagtcagtcgatggcagtacatggat taccatgtaatgaaccctctaagagcagtgatgagaagtcttaccattattagttctgca ggttagtaatggctgtaagtttacattttggttcacagaggggacctttttcctccccag cataattttgtcttagttggtggggttctaacctagctttctctacccagtgtctgccat cctttcctcattgccacttgcctcagacttcctacctgcagtagaggcatgcttgtaatg accagggaaccagtcctctgaaagcagagcaccttttttttttttttaaagcaatgtctg tcagcttgacccactaccttgtaatgggaactagaaagcgaagttgagctctggaaacag agcccacatcctggcaggacacctgttcttactattgtcaccattaatggcagttaccat ttgtagggtacttaatacctgttagacattcggagagtcctcacagtcccttagggtatg taagtatctgaggagtactaccactcattccccacacaactcatgtgccaagtggccgaa ggcacactgacgtttgagccaaagttactcctcagagtctgcgcctcaagttcatcacaa agaaagattagattagatggctccaggtccactccatggtatatggatggcttcctggta ctcaagtgttttgttggttcaataggtaaaacactccttttatttcttagtttttagctt ttttagatgcctctttgtcatgactaatcactaatttaagaaattagttccaggatttta aacatttgtaagtgaatacaagattggtattcacacacaagatttcatatacatgaaaac ttttctcaagtagatttgaacttcatagccagctccaataaatatagacgtggatgaaga aaatagtaaattgggctgtaagctttccactgaaggaaagtaaatttcatcttgactgtg tattagttactattttgtttttcctaaagtcaaccttttatatttttatgacattttctt tacagaaaatataagaataagtttacagtacagttttaagaggaaacaactaataacaca tacatttaaggtagtggtattgtattttagatctaaagtgtgagatagagaaacgcaata cttgttagtagattgagggtagcttgcatggattgttggcctagtgcacaggtgtgtact atattgtgaggtatgctgacttcctaccaaatccctgacaattctacagaaatatatatc ctagaagcaaataaaacttctagctttattttcttctgtgactgtgttaaatgaaactct aaccttcattattgtgtgtaagttctgaatgtgtggacatttatatttttgtacattcac atacatgtgcacatgtgcatattataatctttgcattgactttgtaattgattgtgccag attaccagaggctgcatctctctctgtaaagaggcttttagatagtttgaacttacagaa aaataaataaatactatttttacagttatatttagttaaataaaacttttaaagtctgag ctttatgttcatttacagtttgatttactatggttacaatttttgtataaaatctttttg tgtttttcatatttattttaaattatatattgtgtttaatgttgggtagctatgcattaa tactatagtgttggtttagttggagggtattttggaaaacgagagttcgatatttttaaa actaattttattatgggttctaaaagctccgcttccccaccctgtgtgtggggtgtgtgt gtgtgtgtgtgtgtgtgtgtccatgcccttgtcctttcttattttactatacatttaata gacgaagaaaaagtcttaaattatatactatcatgtgcttgacacattttaagagctggc taatttcctaatgaatgtaggaaatgccctactagccagtgccttgcctactgcagtagt gcatgcatgggtgctgagctgcacacctccttcccagctgatagcacctgcaagagaaca ctcccactgactttccactttagtcaggagcctttccataggaaagttaatgacaacgag ttagtaatgattcaaaaagtttgttacagcctcacatgcttggcattcttttctttgatg tagaaaatttgagctgtctctaatctaattctgctcagggcaagacagctaactgtggta ggtagtttcctgttcttttccagagtagaatgttgtcatgataataagatcactgacatc aaaatacaaacacatcttcttactattataaggtagaaatgtatttttaactgttagcaa ggtaactaagaaaataatattcatggtacaaccaatcctattacagtaaacttggaaata ctaagtatagtagatattaagaaacatatactttatatattcatacatatgcctgaatta ccaaattactcttatggttgtactgcttgcttgaaaggccaattgtttaaaactgttatt tgttacataaatgtttttctggatgtcctacagataacttgagtgatgtatgaaccttcc gggagtaattattagagagaaagttgtacagagataacattaggttgttgtggcagaaga ctaccaagactagttagtaatgacttggaacagtaattgtgtgctgtagggaagccagga gtctgaaggtcatgccaaggaggatagatgcaattcgctgtatgtgcatacttcacttgc atcatccacagtagactgttttggtttgtttatgtatataaccaactaggtgtccagccg tttgctcagagcgagtcatttgcttagactagcggtggttaaccattctatgccattatc tcccagagcttgggctctggttcaaaacatctttgtgctgcttatttctggagccagcat ccacttgtatacaaccaactctaaagcttgctaataggttttaatttaaataatgcttat gcattgctagtacaaggaatatttcaaaagaatgggaaaattattgcctacctggaattg catgaaaatttattttattataatgaaaacatatttactatgttgagatattctaaaatt ttgtacaactgacattagcatttatttatctatagtagaaactcaccagctaatcttttt gatgaacttctacttgatgttacagcataagacataaaatacaaagtaagcttagaacta atagattataggctaaagtcccatgtttctgtccatccaggtgtaatatagatagaaaa aagtatttaaaatatattactaaaacacctaaatatgatttatgcttgagaccatctgta agctaagacagccagaaatcagacgatgtgaatgctttgctttgcctgggtcatatgtag agtctggtagtaaggagatacgacgcatggttttcagtgatgctacttaaaaaaagagtg ctggaaaaatctttatttttattttgttactttgaaatctgattaggaaattggttaact agttttgcttggctagttctttacagttaaattagcattgtaaactaagtattgggagaa ttgtttaaatatttggtaaatggaagtcatcattagtaattataaccatacatatttttc acttaaagggctaagttaataggggccactcctaagtgatcaccttggcagatgaaggaa gtgaaggaagtgggatggtatagactttccagtgtatccttttcatataactttatgagc aagtttccttgtaacttcatgaccataatcagcagttatgtgaccatgagaactcctttg aataaaggtatttctatctctggcaccccactaagcttacccaatacaaattctatagga gagtccaactacttgttaatattcttcagtagcctggaacaagcaagaactccatatttg ctgcccccaccccacaacccctgatcttgaagcaactagttaataagggtctgtatattt tcccaaatattggacacaccctcgcccccaaagcaagaagtttcaatttaaaaagaactt ccctttgcagagagtgaattgattgataggaaaaataacttttgttgagcatgtatgtac atattgtgtctgatggggggtgacataagaattaggtaaatgtttgagaagataatagac agtagaacataatttcttggagagaatctaagatgtgcatcttcataaaatttacatgtg gaagtaaacagtattacattgaatgtcatgtttataatggtaaacattttaaaaattgat atttcatgtaatattccattaccatgcagaactactaaaatatattgttcaaatagtagg aatctattttctaaagaaactaagtgctctaagtaactatgggcacatagcttacttgta ataaagaacattagtggggaggataggagttaattaactgggcactagataatagctttc ttatccaagaagtcttatcaccacttccaagggcagttgtgtagcaagttgcaagatgaa tacctaaatttgtacataatcattctatgagaaaggttgcctttaggcaaagttagtttc tttattattacataaaattcttttttaatgctaagcaatgtgcattgccacaatcatttt tgaggtaaaagtctaccacctcttgttggcccagggtgagtgaatgtcaggggattccca agtgagcacggagcacatggagaaagtagccttgatacaaacatgcattaggtagaagtg gttcatctttaagggtaacccttcttgttcccgagaatcactggcagttatcttgaaaac tatattttaaaataatgtgtggtaaagcttaagaaataattttactaaatcataatataa gagacaatcaggtgaaatttatcttttattacataatttctgtaaaagaaccttttgctc caaactggtttactttgttgtcagttcccccaagaatacttaatttttccctttggaatt gcccatatcacactgcccaagctacttgaagaatatagtcagcctctgccaagtctatcc agtaggatgacagtaatgaaagtagcctgcttcgcctctgttgggggtgggggttaccca aatacatttcatttccaaaatgtttctttctggaaggacttatttgtggtaatgctgatt gctatttaaatctcgtcaaaaatgtgggttttacaaaacacaaaaataatctcctgaaag tttggaaggggagaggggctgtggtgtgggggcggtctctgctgcccacgtccgccctct gttcagtgcgttgtgtgcttacgggctccctggagcagatcaccatataattgatgtgca gtctgcattgctgaaccctggactgcaccattctgtgttcaagggaagatgtaatctgat ccctctgctgctgagggaggaatctgttcagtcaaggctttgacagggcatagtctcctc caataatcttgtgggttctcgctcattattccccgagttctcctcagtggagctgcatgc gtgtgtgcgtcccgagcagcgttctggttctgagctgcgtctgctggtgctggggagttt tgctgccggttccgctgcctaatcttccttttctggcgtgcctgagcatgtccacgttag aatctgtgacatacctacctgaaaaggggttatattgtcagagactgccaagcagccgga cacacgggggcacagaatccctgaaggggaaaaatacagaaaatatgggtttctacggca cattaaaaatgattttttacaaagtaagtaatctgttttcttcctgtgctgtgtggttgt gtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgcctgagaaggaaaatctgt atcatcagagatggctgcagcatcctctaattttggtgggtggttggattctggtattct gctttatttcatgactgttgtaagcaagtatggagcgatggtgtgagctgggggaagctt taaaataaaatgtacatttccatctgagattttttcactgaaaacgtatctgagactcca gaaatgtagctccgttaaatgctgacctggaaatactcagcctcacttggctttccgtga acctttctgttcagttttcctgtaccatagtcatacgtatgtgttccttagttaactctt ggagtgtagtcactgaaacgtgtagggtttatgtttgaagagattggactacttgtggta tcttaaaaaaattacccaattaatttttggttataaagtcagtattttcagttaacatta cagtatttaaaagatttccacagtcattcagaagatgctttgttttgtaaaataatttaa acataaacccttctcagtgaattctgtgatatatgttttaaatattttattcattgaaag attctagatggaaataaagtttgcttttagtggcaattagatgtcatttgtaccagcata tttacaaggcgttctgggaagttctgagagccagcattactgacttaattcagatgagtg tcagagactcccataatgatcaggaaaatcatctgcgaaagttaccaagctctgtactgg ctgcccttcctgatggattcatttgtgtgtaggtacatgtctgagtatctgaattctaca cgtggaagcacatggctccctgtgcattacagcttctatattttttgttttctgataaga ttatttacaaagagaaaaaataatatatttggagattatcttttaaaaatgaagtgagta tagaaacttccagagggaagaaagcaggccaattctgtttcccacagtaggtatttcact aaagtataaattgtgcataagcttcttgaaccttcgcagccacagatgacccagtgttga ctgggacactttcttcacgataacaagtccttgctatcccatcccagagtttgaatcttt ttaatgccttttagtatttacagtgtatatactatgttccatgtgttcctgtgtgaagca ataggtaggaaaatgagcctactgcaaaataggggttccagcttactataaaaatgaaga tagctatctccccataacatgcagtaatacagtagctagtgagaccctgcaaatccaaga gaagctgttgaaataggaaaagttgaaccttaacagattttattttgtatacatatactc agatgacctacgttaattgatagtgaaatacaaaaataaatatttcaatattgacattga caaatgcaattttgaatgtattgatagcacaacatggaatagtgagaaacctttaagatg tacatgttagacatgtctcatatatcatatgtcttagtcataactcattcttttacaatc atttcgttgtttcaagagtattttttcctgcgtattctgtatgtagcagttgatgatttt tttccttcagctttactgtggcagatacagattgcctgctattagggatttactcttttt aactgtctctgttatccttgctaaactcttctttattttaatcactattcaagattagct tattttgctctctacctttgtataatgatatgaatgcttaaaattcagttattcatattt ctggttgtgaaatttaaaatttttatcttactatttttaaagaaaaatgattagtaagga agacatactaaatacagttattttctatgtgtgctaataaattttttcttgttattaaac tgaaaaatgaaaagtatataacattctaagggcatggtaaatattagtccctcacctaac actgctcttcccagtagaatactaagggaacattacctatccagtccataagagtaaagc aaataggaaaaactgcgtaatatattttctgattaacctactaataatcatcattttaga cacatgaaaatttaacagtttttaaatattagcatgcaaactcaaagtctgataatggac caagtaattttttttattttatctgttttactttatagatgaaaagaaagttggaccatg gttctgaagttcgttccttttctttgggaaagaaaccatgcaaagtctcagattatacca ggtaacgttgaatagaggggtaggcgggcatgtcatctggaaacatttctttgaatttgt tttcgaagaataatagtgctactgtattcagtgttgttggttgttgaaatgtgttttaac attacagaaacggggaagggcatgaggctcaacccaagcagaagggctggcactgtccat cttggactttctcactgatactctgatgggagacacatgctggagttctttaaacaagat tgcaaagtcacaattttaaggacttctaaacagtaaaccgcgaggaaatgtctctggtta ctatagcaactgctgttttagggtagctactttcatataccaaatcgaagataaatttat tagcagtgaggtaaatctcccggttactaaaacttacttcatggaatgaatgtattagaa atccccatttattggcatgatgggttttatgttttaacacatacatttctacacttgtag aatagtttccataacaactaaatacaattgtagtcttgtttccttcttcactccatcatt tttggattttgcgtcagtataaaactttgcccactctcacgctaattagactaacttaag cacaatatctcatttcccagccaattaaacatggttttggatgtcacttgaaatgctgaa ttttaacatttaaatgacaaaagtatctaagaactctgtggtcccctcccccacttttgt ataactgtcacttttattgaataactggtcagagtgaacagtagaaattccaaggacatt atggttaggatctttttttaaaaaaaaaaattttgatttagttatttttattctgtgaaa cagaataaaattaacattgcctagatacaagattttctaaaacatcattacaaattgtca cacaccccatacatacagtttgtttttattttgttcaattgtggaaatatatttaatgct cctgggtaatcaaggaatcatactgcttaggataactttacatacagaaagctctggcaa gatgatgctaaagagatgaagaaagcttcgttctctgtacagcccttctcccctcccctc tgctgtctgacaatttcactgttgggcgcttgcttccaccagagggtgtggagggataga gagacaccgcaaatattcacgtcattttggttttccttctaaccttgatgatatatttct cagatgaggaagttgtttgatgttaaactaattgtaaatgtaatttttttaatgcacaga tttcagtctgttcacattattttggtcttctattaaagacaataccaaaacaaaagtatt aaatgatcagctgttttagaataaaacttttggtacagaacacattctcataaaattttt gttgcctactttttatatcaagacagcaacagccttttaacaaggaatcctactaaagga gaattagagtcaatacatatttatttccatgtctttgacacaattttgtcagttagtaac atatatgtttgatattcagtaatgattctgacaggtttttttttgacaggtaataagata aaactcattttgtattctcctgcctccttaggagactttaactattaacacagcctgttt tattttggagaaaaagaaactgaatttgcttttggatttgaatgaaggaaagattgattt gctagttgaatattagagattgttgtaaataaatattaaaaaattcaaagggtgcaggca taaagatttttctataaaatagtgatttatgtaccgttttaaaaaattaagagtttcttc attagcgatttgtcagaggttgaaaatcagttgttatggtacaggttacatgtgctcttt gtgtttcttttttaattcttttctttttggttttgttttgttggggtttttttgttggtt tttttttgtttgttttgtaaacaaattcactgtaacccagcctgacttagaattcactat gtagcttgcctcaaacttaaaatggtctttctgcctcatcctcaagtgctgtgataacag atgtgagctgtctgctctttggatttaagatactttattatcatagttcagtgagctgag gattcttccacaggtctagttttgttgaacgttaataccacctgtaaggcagaaatgctc acattacctggtttgaggtgcttgaaccagcttagaggagccaccagcgtgtctgtgtgt gtgtgtgtctgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtgtct gtgtgtgtgtgtctgtgtgtgtctgtgtgtgtgtgtgtgtgtgtgtctgtgtgtgtgtgt gtgtgtgtgtgtgtgtgtgtgtgtgtctgtgtgtgtgtctgctacagtctcagggctgga gcagaagcactatactctgttctgagttcatttatttagtgtttttcattatctcagtta aattattggataagaatgtttagttatctcttttaattttgtttgaaattctctatactt tttttgaaaaactatgtctcatcgaggctgactactaccagatacctaggctcagcttaa taaatgacaaattaaaattagcaatggataaaatatcataaataatttcaatgcatctgg ggcaagcttaagaacttatgtataaccagtgaaatataaaatattttttttctgaaatat tggtaacaatactttcgtacttatttttacaaagaaaatctaaacttaaactgagtgctt gaagacgagtcatggaagtaggagctgtatgttctaaagccatgtatttttgcctgcata cttagttatgtgctcacttacggttagggctggacctggcctgtgttctattactgattg cacctgcagccctaagcaaatacactagcaacttagagctttgtcatttggttgccagta gcattgctggatggtgataaggaagggactttagtgaggaagaggaagaggaaaaagagg aggaagaggaggagaaggaagaagaggaagacaaggaggaagaggaggaggaggaggaag agaaggaaggaggaggaagagggggacgaagaaggaggccaaatgtgatggcacatgcct gtatttctaacactgaagagtttgaagcaggaagcttatgagtttaaagctagcctgggt gacacaacaagaccatctgcagagcaagagaaaaagaagaatagcaaagcaggctttggg tgtccccgccctgagagtagcaagcgaaagggcttgctctactgatgtttgaagaacacc agatcattattctgagtaaattagagttcatagtttttaattaagacctttcttccactc ttacacctaggctcagcttaataaataacaaattaaaattagcaatggataaaatattgt aaataattttaaatacatctggggcaagcttaaggtcttacgtataagcaggaaatccaa aatttttaactctgaaatattggtaacattaatactttcatactttttattttattttat tttttagtaccactggccttgtaccatgttcagcaacaccaacaacttttggggacctga gagcagccaatgggcaagggcagcagcggcggaggattacatctgtccaaccacccacag gccttcaagagtggctgaaaatgtttcaggtgactacgtgaacacactgttgaaatgctt cgctcgtttgcttggggtcagggtgggagtacagaagaatttggccattaacaaaaaaag tagtgtgtctgatgctctaccatttatgagcattggaaacattggactaaatgaaagaag ccagatgtaagcggaaattttattaataaaaagggacagaaagtaggttagtggttgcca tgtaggaagcagagaggcctggggctacctgctaagagatgtagggtttcttttgggaag ggtgacagaagtgttctagaactgggtagagtcgtggtcatccaaaaagacaggcatcct gggacccccctaggtgtacacttaaatagcacgcctgttacacggcatgcatcttctaaa accgaagcacagtgcttattttgtgtaagtgtatatttgcatggctttattggttttcta aatagtacctttattttcttgtttgtgtttgggtttctatgttgtggaatgtattcttgt ttttggagcacatttcctgtgtattaggaatgtgaggttagtttaggaactccatcagtg agtcgctgggattggtaacttagcattcactgcttctcggtagaggtgactcagtgcttt ctttcattgccttgcttgcagatgccttgagttcgctcaacttccaaccactgtctttta gtaggatttaaattctttgaacatttactgggaagattgccttgaatactgtggattttt ttaatttaagttagttagattgtcttatctcttcttattctgaatgatagactaaatcaa gaacataccaacacagctatcatagatgaattacttaacaattctttccagacagtaatt aataaaaatatgaacatgcctgatgctagctagccaccacaccaatataaaatgaacaca tttcactacctcttgtctgcttgaattgttgggtacataatgcacacataagaaaattac aaatttaaaagatacattggctttgtcatttcaaatatttaagaagtcccattgtataaa tcctaatattttaaatacttaggtgctgtgtgtgtttgtttcaactatagctagaaaatt ttcttgaagtgatgtctttttaggtgagttttaacactactttatgggatggggcagatc tagctactgtaactgacaggtgtggagccctggttctcagtagggtttgttttttgcctc gcaaataataaggaaggggttggagccttttgatgtttactgtgcaaaagcaatatgaag gatagcctccaaaagcaaataagtatccaacacaaaatattatagtactaaggttaagag tcttgggattaaacaaatggaagttacattttacttatgaaaatgaatactaccacaaaa aacctgtttttaatgtttatagacatgtgttaattaaactcacctttcctgtaagggtta ctacttccccactcttttacctcaaatttatgacattttccccctacctatgccctgcaa attctgaactttttattctgtccagtcatttagaaataaataaggcttgaaataaaaatc tagacatgtttatatactcttaaaggaatatttaaaatactaatatctgaccaagccagg gaaacgggaggaatgacaatgagttctgcatgcacatgaacatgtgcacaagccagcacc cagaacacagccctggggtcttacctgggtgctgaaaccacatggtgtctctagtgccca tttccacagccctcaaaactgactggaaaagacactcattagcaggctattccaaataag gttttttatatatggttaggcccatttttcctacagcattttaatgtaccaaaatatttt ttgtaaaccatttatgatgttcattaagagaattctttctaatattgatctgcaatagtg ggggtattaatgctttcttctcatgaggctgttttagagagccctttatacagtggttat ttaataaaatttccaaatgttatacttgttacagctcagctttagaaatgttaactataa aagtaaaatcagaacacccaactgtgagtctctaaacccatttggtcctagaaaagtatg tcttttatatgagtagttgcagtgagagatcattcttttattttatttattcactttaca tcctgattgctgcccccccccagtctctccccacctccctccccttttcttctgagaaaa tggagtgccccctcccccccgaaaacccagcacatcatgtctctgcagggctaggtgctt cttcccccactgaggccaggcaagacagctgagttggggacatattccacagacaagcaa cagctttagggtgtagtaggattgaggaccctgaaggggataggaactccacagggagac caacagagttaactaacctggactctcagagactgaaccattaactaaagagcatacatg ggctggacctacaccccccaccacacacatgtagcagaagtcagcttgatcttcatgtga gtcctgaataactggaaagagctcatttttagttcatagaaacaatgtagaattatctgt gcaagtagaaaacgtaatcctcctgacccaagacattctgtagcaactaaacgtcaacct gacaaggtagatccagccttgatcttctgagcttcaagtctgtctctgacataacccagg actcgttgccatacactgcccttgtcttagctagagttttattgctgtgaagagacatcg tgaccacgataaaggaaaacatttaattgggactggcttacagtttcagaggctcagtcc attgtcatcatggcaggaagcatgacagcatgcaggcagacatggtgctggagaaggagc tgagagttctacatcttgatctgcagtcagcagaaagaaactacatgccacactgggcat agcttgaatagataaaacctcaaagcccaacccacagtgacacacttcctccaacaggcc acacctcataatagtgccactccctatagccaagcatccaaacacctgagtctatcagag ccatttcccacattgttctactggctaaccagttttgtttcaatttgacataagctagtg tcatgggagaaaggagcctcagttgaggaaatgcttcaatgagatccagttttaaggcat tttctctattagtgatcactggggaggacccaacctcttgtgggtggtgccatctctgag ggtctgtaagaaagcaggctgagcaagccaggggaagcaagtcagtaagcagcattcctc catggcctctgtcagctcccaccttcaggattctgctctgtttaggtttctgtcctgcct tccttcaataatgaacagcaatatggaggtatatgctaaataaacccttccctccccaac ttgctctttggtcttgctggtttgtcacagcaatagaaaccttagccaagacaaccctgg ttttgttgttgttgttgttgtttttgtttcgttttgtttcgaggctggtgccaaactaac tagaactgactcacttgcctggaggcttatgatgcgtcagcgccaatcttatagatagcc ctagctcccgaacaaaggagggtggtatgagctgacctatgaggggtacactggcaatct ttgtacaaatctgcgattgaggcatttgagggtgacttccctgttgtgtaacaagcaaaa tgggagctcttgtcctcttcctgccctcctattgctcctaaggcagtgttacagcacttg gtccagcctcctctgtcctagtaaagtactctcaactccatttgcaaattgagtaagaac aaacgatacaccactgtagataacagcctgttaatgaaaaacagaaatgtcaactaaaaa caatggactagaaaaaatacttctgttttgatatttataatgaataaactatatttaaag atttaccatgttctatgctaagtcaaattacacatgttttctgacttgttttacttaatt taagtcttgttagtaacagaagattaaaagctataagagaggtgacaaaacaagatcagt atttttgtttctttgaaattttgttacagcatggttaaggaagcttattaaaccttgtgt aacgaagttgaagaactttgtgtatttagtcagggaagactagaggaaagttctgtattt aatatagtctttacatcaaaatagaaacagattgagccttgggttctttggaaaggaata cctagatcacgttttctgagttggattatgaagctgtttgaactaagaagaattgcccac aggagagggcaagaactgttcaaatagagatggggaaactgggtgtggtggcttaagcct gtgatctccagtgcaagatggtgtctcaaaacagcagtcaggtagactgactgccctgct taggttctaaagaagtgagttttctatcatgggagttcaggctcagtgctgtccaaggtc ttaaaaccttgtgcctgattggaacagtaagcacactctattagtgttccctcgtgctaa ttcatgaatcacctgcattgagagagagagagagagagagagagagaaatcagcagggca tgttgatacatgtctttaatccctgcactctaggggttgaggcaggcagatctctatgag ttggaggccagccttgtctacaaagtgagttcatgacagccagagaagcactttcttggg ggttgggggtggggcatctccttcgagtaaactgtgggatcctgccagttttggggaaat cactgtgccagaaaaagtctcttattatggaggcttaaggctgtaagtagaagagggact ttgcctgggaaatagaataggagagtaaagataggaagcttgagagaccatttggagatc tgaatatcataatctagaagaagtgctggtagcttagctggtacagtaatgactaagtct tagatatgttttgaacttcttgaatttgtctctgagcctcctgcatctgcattctgcttt caaatgcttaattaaaattgcagttttgttgagttagcatagttgttcatctcccaaaat ggccttggctatggtattgtctacagatttcttataatgtaggataggtaaaataaccaa catactatttttttaaaaaattcaaattctgtgagggacgtgaatcaataaattgtaatt ttaagttatttatactttatggtcagtgatgaatcaaatttgaccatgttgtcagatggg cagaatctgtggcaatgcctgaatgctctgggatcagacaaccaatccagtgctttggct cctgggtggggcgttcttcgtgttgcatttcaaagctgtgttgtgtagagattgagcaca gagccctgaggacaagtgaatgctttctacattgtatgtgcaagagaaaggagagcacat gacttactggagcccccccccctccttacactggcaaagtagagtaggtcacggacatag ttcagtgctaacccgagttccatagttgttcacatcactgcagagtctaagggaatacat acctcttgaattactctgagtgccgcttcctgcaagcctttgtgtttcttacatcttatg ggatccgagtgcaagttttagaggaagagccataccttgcctgttactctttacccatcc atgtccaaaatggtgcattagcccctagaggtgtcttcatcagaggcctgtgttgatatg ttttcacgtaacaattgtgaagttacttaaatgttttacatggttattataaaattccaa catcttaaattattcatatggccttaaatttgttcctttaaacagccatggcttttgtga gcagtcatgctcactgagggaaaatattaaaaacaaacaaatgggaagaattttttaaag gaacccatacttttgaagtatatgtgttttcatttttcataagtacagcatttttattca ttttgggctttgattatattcaaattttgcagtattaactcagctaatattttctattgc ttgccattttttaaagtaaagcatttttaaagtgtgtagaagatacttgtcagatatgca gaataaccaactattaatttttttatgttgcaaatctcttagctcttagatgaaaacctt attcaagccataaaattttatggctgtccactaaagagaatatatagagaaaaatgacct atcaaaattccctatttattcagtaacccacatggatgtttctaggacaagattttcgtg tctcccaatgtcttggtgttagaagaatgcataactctcataattaaaaataaatgcaga ctttggtttttttgtttttgtgttggtttgtttgtttgccagtcatgatttttaatgctt tttgggaaagttactgagtaaacttgctgaaactaaaacctgcagtcgcttcagtggctt ctgtccatggttccactgctcaaacatggccatgcaggggcttgcgggtcttttgtatga atttgtaatgtgtccaattcctctttggtagagctggagcggaccagagaagttgctggc tttagatgagctcattgacagctgtgaaccaacacaagtgaagcatatgatgcaagtgat agagccccagttccagcgagacttcatctccttgcttcctaaagaggtaaggggctgtca accgcatcccgctgggtgctgtccagtggttatgggtgcataaccacagcctgtaagcag tgtcctgctcattatggttgacacttggacaaactgctatagccttttggtacagttgtc acataaagtgcattcataccatggtactacttccttccacttggataaagtaaggctttg tgtgagagtcactgagtttcttcaagaattttttccctaggttatctaaaggctggcttt agttatttggttatttttttagttatttagttgtgtttattatttatagtggatgttttc aaaattctgggttcattaagcagcaacattatttttgtttttattttgttaattatctaa tgccccttcaaatcagaaattttataattgttgtatttctctggttattacattatacaa tttttttaacatatagaattttacctatattcctttttggcatttctactaatcatcatc catcagagagagaaagagaggagagagagatgacataaaaattggattcttacagctctt aatcatatagccatgtgtggcttatagtttaaatataatgcacgtattgacattagactt aatagcttaatttttatcaaaagaaaggaataatgtgatattaaatagtatcctcatagc ctatattttttaaaattactatatacatgtaataaatatcagttaaatagcacaaaaaac taacacatcattcatacaatgatcctgaaccctcaaatttgaacagaaagagttttgctt gctcttttcttagataattactctaaattcctagtggtgttaaatatagtcctagatata atctggcattagatttgagtaaaatcagtggagtgaagtacaactctggggaaaagcaaa attgatattcccccccccccccggtatcttttcccttttggagtactttttaaaaatgac attgtcccagaagaaaagtgtttattcttgtgccctttttctacagcagaactctttgaa taaacttagaaaagtaacatgagatctcctttgcttccatcggggcgtaaaataataggg gacattttttgtaagtgaagtagatattcatcttgattattttacatggccgataccctc ctctagaatatgcatatgtgtagtatgttcatgtgttcaagtgggtgtacatatgtacat gcagatgtggtctaccactgaatcgagagcttacccattcagctagagtggcagctgtcc agtgagcagtgactgtagctagaattgccatgcctagccttttacaagatgggatccaag cccttatcttcatcattacacatgaaatgttactgactgagtcatcaccaagctccagaa tttgaaagtgttttagaatttaagaagtaagcacaggtgattaacaaaaggcaaagcccc aaaagattctcttgccttcggtgttctttcaaagacttcctgaggaaaaaagatgttcgg aaggagagagccctggcttagcatatcagctatggtgttttgtttcctgctactggggtg ttcttcacttgggaagtgctttatgtctatgtggaagaataatggtataggcttaaccct atagggaattatgttatttaaaactttattaccatttattacacaaaatgtacttgtata tatatatatttttgataattggtgtgaacaatttcaagtctctttatcaacatttattta ggttatctaagtgcttgagcactaggctcacacaagaaacctgtgataagtggcattgct ctcaccttgtaagtaaataccaataaaatcacaggtcacccagaataaagcctcagattc ctaatatgagattagtgagcattttctttgctcttaaaatcctgcttttcttgatttgtt cagccactgaaatgcagtagtcctagcaagatgctcagcctgggtagagtaacctttttt tctgtgattttttttttttttttttgaagtgtgagtatgccttccctatttccttttcca tagttggcactctatgtgctttcattcctggaacccaaagacctgctgcaagcggctcag acttgtcgatactggagaattttggctgaggataaccttctctggagagagaaatgtaaa gaagagggtaagttcagaatcacacacagatgtgtttctactctggaaacaagttaacag aataacttggctgtgagtgagagtagatggctggctcaggtgcctgccagaatgcgtctg cgtctgttcacttccttgatcccaacaggaaaggaggccacctgatttaaaggaagccac aaatctgcagttaaatacccacagaattgagtattgtatatactcctcgaataaacactt actgtgcacctcctgtgagctgaacatttgtaaatgctggggacatggcactgcttcatc ctagtctccctgttgtggaccttctattattaagtatagtttagtttggcttcaaaggga gaatcttctgttttgtaaacaggtatctagaaaagtaatcatttagaaaaaaatagctgt ttctgaggggcaaaatgcttgaggggggaatggtaatgctctgttctatagatcaggccc cttggcagccgtgtgaccaggtagagagcactgacgagtgaggcggaggagggaagactc cagggtaggatctcaggtgcttcttgctgagcctggttctacagtcaacctctggctgtt cctagattaactgataagatcctcttcttaagttgtgcgggttttttgttttgttttatg taaaccacggtgaatcaactctgccttaccttctttctcagaatcttttttccccttgag acagggtgtctctatacagaccaggctggccttgaactcatgaagatccactttcctctg cctcctgaattaaaaggatatggaattaaaagggtatgccacatacctagcttaagagtt ctttttaaatgtcctcttaaaaaactatatttcgtaaaaatgattctgaacagcttggtt tatatagcaagttccagggcagtccagactgtgataataacctgaggtaatggggttcat tgtactcatagcacttaggaggcagagacaggattgctgaaagctagaggccatcttggt ctccatagtgagttcaagccattttattgagtaggatgcagtgatattctccccattctt ccattcctgttgcctacatgctttataagtttactgttgtaggaaactatgataagtgaa attatacaacatttgtccttttgtgattgccttattcactgagcaaaatgtcacagcgtg tatccccgtgtcctctgttaaggcgagcagcatttcagggtgcactgctgcatttggtgt tcatctatggaaaggcacggtgacttgtatggacagccgttcacatctctgcttttcatc cttttgcatatacatgcagaagtgaaagtcctgcatcctgcactgcgtttgtgtatttag gagtcactgtgctaatttccacagcagtgcacatacagtctcatctttgcgtgttcatac cagcacctcccacagtcactgtgagagagaatggagatagaacaaatggaggttcagtgc tgcacagcccagtatctgggccaagcagagaaaacaagaactttataagatgtgtaattt ttattagtggtgaaaagaattattctcttgaactctttgatactttagggattgatgaac cgttgcacatcaagagaagaaaaataataaaaccaggtttcatacacagcccatggaaga gtgcgtatatcagacagcacagaattgatacaaactggagacgaggagaactcaaatctc ctaaggtaacagactcttgacatactaacagaaacctttcttcaggaacaatgcctgctc tcttcatgtgttggctgcagtgtaactcagtgcctatcttttatggaaaggaagcagctc tatttcaaaattaactcagagtgttaagatcagtgttcttttatttctcatttctttaat ctgataattttattgtaatcaaggatgagttgattcaggcatggtaccacatgcttgtag tccctgtattcaagtggccaaggcaggaggattactatgagttaaagccaacctgagcaa tatactgagctctaggccaacgtgagctatagtatgaagccttgtctcaaaaatgtttct ccccaaaaaggtgagttgagacagtgtgtgatactgtgctaccatgattctcactatgag tttgcagccttctagcctcacaggccaaatggatttttttttgtgtttgtttgtttttaa tgatttgaagcagaagtctcattttctgtcttagaatattatttcagatgtgtaaatggt agaaatactaaatacatactttaataacaaaaagtgagccagctatgggaggcataattt tatatgccagcatgccctgggataagttttaggttaaaaacaaaaaaccaaaaaaacaaa aaaccattatttgctattactctgtttcttggtgctttatttttcttctctggagtgcta aaagttggatctaaaacccactcttctcctccttttcctcttccacctctttctcttcct tttcttctcctttactactgagctgaggttggctcagttatccaggctgccctggagctc accttggtatcccaggcaggccttaaccctgggaccctctgttttgtcctccttgagaac aacagtcatcaggcctggttttcttctgtttttgtcaacagtatttactaaaataaagta acttttcttttgacttaaacagattaggcatttgtgttagaaccatctttatgaagtttt ggcatagtaaaatgttaatagtgggcaaaggaatatggaaatatttaatgtttaatttat catccaggaaaaagatgttgtgtcttatataaagttcattctaaaaatagattatagatt actattctgacgcatggctccttcttgttttcactgtgtgtgtgtgtgatgtatgcacat gtgtgtgcacgcacacacacaggtcagaggaggtagtcagttttcctgttctcattctct gtctgacttactttttgagatagtctctctcactgatcctggagctaggctagcaattgt aagccccagcaacacgcccctcagtgtcagggttattgtgtgaccatacacagtttttct atgagttggagagatttggccctaggtcctgtatatctgagccatctctacagtatgtca ctgtattctgaagaatgtgatatagtaacatccatgtgatctgtacacttttcttatagg tgctgaaagggcatgatgaccatgtgatcacatgcctacagttttgtggcaaccgcatag ttagtggttctgatgacaacactttaaaagtttggtcagcggtcacgggcaaggtaagtt cactcacttctttctatatgtcagggagtcaccattttgtgtgtgtagtcacagtaaagt gggtgtttcttaatagcaaatatcaggcctcctttagaaacacgtcccttttcctgatag ctatgagtgtcaccctggcatgctggcagcagtagggtttccttcatttaagtagctcgt gtctttgtcacttagtctaagatgtcccaggagcacatccttgttccttttcctcagcac ctttcaccttgcctatgcgagttagaatcactacttcctaacgccttccaaaacatgacg gagaaatgtgcaatttcccttatatgctgctttgttgctgagctgtctgcaatttagaaa ccaggcacatcctgttaaggttgtcgcagacatcagtggtagggaatgaagcagggctga agagccaagcagttgaagcagcggctgcaccgttcatacaccatggaccgttccttaacc agtctctagtgaatattgaattatatgttcaaaagagaacattttcatgaactaagtaat tgaccatttataaaaataacacaagaaagtatgtgaaaagcttatgtttttagaaaatat gtcttgctgtgttgttccttggacaagagtcttacaaattctcaacttagatgttaaact gttcagagcagctatatatttccagggtataatagtagtttgggtttatctcggagtttc agcgtctgtctttacatgtctagaagtagcataggcagccctaactactgcctttaggca gatggggagagctgtggtatgtggccacacccaacgctccctgcattcttgccttccttc tcgtcctggaagacaccctacagctcagcagtattttagtcctgcggccaagctagagtt gtggaacttgcctgctcacagtatggttgcttcctggcatgtcttatgctttgtagcata tgattctctttaactttctttacttcattttattatatgcataagtgtttacttgtatgt atgtctgtgcaccacatgcatatcaggtgccttccaaggccagaacagggtgtcaggtcc ctgggcactagagttccaggtggttgggaggcaccatgtgcgtgccggagctcaaaccca ggttcctgaaagagcaaccagtgctctaaatcactgagccatctccccagtcccttccta tgaatcttacggtttataccatgtttcctttctccatacttgttcctcagtcttgtatcc ctgtataagcatattctgctaccaaataagctaaaggtagtgatggtgtcagatagcatt ggtgtgcaaaaatgctttcctagtgcttcagtaaagttgaataagtaaaatttattccag attttaatgggatgttagtgtagtctttgacttacttgcatttctacctaaaattaatca tctgaagtgtttttccagtgtctgagaacgttagtgggacatacaggtggagtgtggtca tcacagatgagagacaatatcatcatcagtggatcgactgaccggactctcaaagtgtgg aatgctgaaactggagagtgtatacatactttatatgggcacacttctactgtacggtgt atgcatctccatgaaaaaaggtaaaggatgtgccattatgattggggctcgcattactgt tccttgtgactctgggattcatagagtgctaatgagctcttggagtggacactaaggata tagtgcagatggtccgaaattaagaaaattgggttatattggcttaaaagtggaggattg tctaaatttccttcttcatttgatatatggctgtttttcttcaaactctgctctctaatc tgttttgatgttttgatgattcatcatctttaaacaaaaatcatttcctttcttgatttt cgtattatgctgtgtaagaacaacaaattcagtcaccaggccttgttgcctgagtcctta agtgacacacccactctgcccccactccatcacatccctgcatatagacacagctaaggt ccaggggccttaacttaatgtcagtactagtcatccaaactattgagagtgctcaaatat ggggtcctcttgccttttgcacagctgacagagcgtcctcatattaggatggcttgctgc cctacttctgcccaccaaatgaagggagaacttaaagtcccgttttcaaggtagtctcag ccaagtctggcctgtttgccctttccttcccagttcctctgcctttatgtcaggcttctg ctgacctcaggcccttgtgttgatacttcttcactgcacccagctaagaagttgtgtttt ctacttctttgtcacttttggtctgacgtgtcacacacatgctgctaatctttgcactga aaaggtagacatgggtggatactgagttcccagcttttagaggagagccttcctctgacg ctgtagcttagaagggtccttctgtggaatccacagcattcacgacctacagctctgtcc agactgcttttcattaagaatcattgctgtaagcagaccttccctacagccatcattgta cagggactcctctcactcataaatttgagcctgctttgcacggtgtgttctgtgattaaa atttagggatgggtttcaaaggacaccacatttccgttttttgttgtgttcattctccca catgtcagcactctgctctgtctaatgtagaatggcttttttatctcttgagaatgatgt aaaggtgcctggttagtaatctagcggtaactgcatgattgtctcgctctggttttcaga agagtatcattttatagcactgagacagttgctttaggcatttgtgttactgctatagga atatctttaagaatctctgagtcaccagctatgagcacgtgtctgaaactgactttccct gactgtgaagtataaggaaacaactatggctttctgagtcccaaattcactttaaaattc tattttgttgagaaataattgtattgaaatgtctacttactcaaatagttaataggagtt cataattgcagtgtagtgaaatgacaaagatcataaagaaggaaagttctggattgttgt aagttcataccacctggaggtgcattgaggatttatgcatatgccacatttccattttac tgttaatcaggatcttgttgtgtgattggagagctgcttgcttcttgaatgcagtgcttt ccagactgtgccatggaaccttgaggtggaaccagggtccctaagtcagggaaccaatca gatggccagttgatctccaagtcttcctcacttcccattccaggcagaggagctggaatt tgcatttgaaggcattaggtagagtttctcagaaaggatttctgtttaagaaaaagagga gaaagcaatggtttacctgtcctgtgcattcatttcctgactcttgtgttctgttctgct ctgcagggttgtaagcggttctcgagatgccactctcagggtttgggatattgagaccgg ccagtgtttacacgtcctgatgggtcacgtagcagcggtccgctgcgttcagtatgatgg caggagggttgttagtggagcttatgattttatggtgaaggtgtgggatccagagactga gacctgtctacacacgttacagggacacactaatagagtctattcattacaggtaagagg ccgtgtctctcccaccctttgattctctgccgttgactcataagattgttctcctcagtt actgtcaagttgctgtttataagtaccttaaaattagcatgaagaaacacaaatttcaaa tcatttaactgatttaatgatttaaatttctgtgatccattttctctttaaaatacaaat atttgtcttggaacctgatacttcaggattataaattttgctacactaattaatacttag ataatctcataggagaataacttggattttaataaactccttagctgaggattaatttga ccaaaagctgcccctttgccttgatagctaaaagttgatggagcatgtaattgtgatttc atccccatttgaagtagctgtggaacctctctgcagaaagctctgtacttaacttgccca gagccactgactgggagccacagagagatgaatttgactaacctgttgttttcctttaga tttgaacttgatatttattaatagactgtaatagttcaagatcttcagtaacaagacata aaacgtgggatacttttatttctggaaatgcagcattttccatcttaaaatatttcctgt tacataatttccttcccagttactactgcctgttagtgatgagcaaagtagaacagaatc ccagtgctgatgttgccgttcttagggcatttgattcttaattgatgatactgcccaagt aatgaagtattgatatataataaagatgttgattgcttcagaaaagaggatggcttccaa ggggcgggaattggttgctaatgctgctgcttgtgatgtgctcagaactgtagagaatga tctcagaatagatagctgcatgtcatagagaccttctggggactggtagatgcttattgg cttttagtgtccttgggaagctgaagaagagagactacatagattgcgtttgtttctaaa ataccccctcttctaattcctgatacagttttcttttgggatatttgctaattagcatta atttcttgtaaacaacttcattttgcaaacactttttaatggaatttactattgaacatc atgaaagttatctgtggctattcccttgcctcaggtgctgataaacatgtctctgtctca ggcagtggctttggcttcctctagacaagtcaggctccatagtcctactaaatacctggg atgtagcttccagctgggaccaggtggtctgaaaaaaatgttgtttatttcaggtgcttt gttgccttcttgaagttgtttctgccttgtttgaacgattcggctgttttgttttgtttc tttttttaacttttaagagaatttatgaaagaagtattttgaagacatgatctatagact ttcatggcatgcacatcagcttacctgcaagtttatcccagtaggctctttgttctggcc tacgtgttgcttgtaccctctccctattgtgagtaatgtcaacagctgataggacaggaa tccgtgtttctgttctaatactctgtgtctgttgtagtttgatggcatccatgtggtgag tggatctcttgatacatcaatccgagtctgggatgtggagacagggaattgtattcacac gctaacaggacaccagtcattaacgagtggaatggaactcaaagacaatattcttgtctc tgggaatgcagattctacagttaagatctgggatatcaaaacaggacagtgtttacaaac tttgcaaggtaagtcctggccctctgttagcctatctttaaaaacatcattgctattcaa gagagaacctgtggagaacagaggtttaaccttatgcagagtacttactcctgatgagtt tgtgaatgtttggctttcaaaagtaagccaccttgagaaatggttttcaattgtgtcttt aatgtgaaaattcttaatcatttcctgtgtgttctgttgactctgtttaacatggctgtc tcatagggcagcgttctttttgagtgatcattttattttgaattgaagcatctctgatag gaaatgactttggccttgcttggtcaattccttcagtcggaatggaaggagtaatggtgc tctctgaggggaaggtagagtgagaagcattagcattaggtatagcaccattggcttctc catgagtaacttccttctcttagtcacagcccttagaaggtgttgttgttgtttgtttgt ttgtttgtttggagtgaagccatcaggaggcagcacatagcagcctttgttcattaattg tgactgttcctaggagtttggggtagtcacaaattctatttccttctttgactgatcagt tcagtgtggcagagtgacagagtgacaaacctgcacgttcccttttccagcagtggaatg tgtacactggctgaccagggccactgggagccggagctcttgagacttggtactgtagtt acatagccaccagatttccttacatctaagctggtcctaaactacagcaaggttgctaac tgtgcagctttttaaatactgcatttcttgtatgccataatgtagaagagcacaagtccc acgcagaagccctgaacgtccttcctaagcagtaacccgcatttgcctttgcctaggtcc cagcaagcatcagagcgctgtgacctgcttacagttcaacaagaacttcgtaattaccag ctcagacgacggaacggtcaaactctgggacttgaaaacgggtgaatttatccgaaacct cgtcacattggagagtggggggagcgggggagttgtgtggcggatcagggcctcaaacac aaagctggtgtgtgcagtcgggagtcggaatggaactgaggaaaccaagctcctggtgct ggactttgatgtggacatgaaatgaaaagcagacatgatgaattttgtccaactgtgtag acaatatactccctacccttccccctgcgcaaaaaacaaaaacaaacaaacaaaaaaatg aaaaaaaaaaacagaaaaaaaaaaagagaaaaaagaaaaggaaaaaaatcccttgtactc agtggtgcaggatgttggcttgggacaacagactgaaaagacctacagactaagaaggca agaagagacaagagaccgtaactgacaggaggcggcagctgtcgcatctcgcaaaggcct cacttgtgactgaggggcagcttggcaagacgactctctaaatccaaccaggtgcaatta ttctttgttttcttctccagtggtcattgagcagagctacatcagcgttgttaccgtcac ctagaaaggagtggcagtaatatccaaacacgggctgcttatcttctaatcagagcatct gcaacaaaccgtcatttttctgaagtggaaaagcttaaaacaattactgtgaattgtttt tgtacagttatcatgaagctttcttttttctctttttcctgtttctcttcttttcttttt tcttttttttttttgccaaccattgccaatgtcaatcaatcacagtattagcctctgtta atctatctctttactgttgcttctactcttcaatgcatatgttgctcaaaggtggcaagt tgtcctgggttgtgtgagtcctgagatggatataattcttgatgctggtgctagaagtag gtcttcaaatccggggtcgttgtcccacccctgtactgtactcccagtggccaaacttat ttatgctgctaaatgaaagaaagaaaaagcaaattattttttttttattttttttctgct gtgacgttttagtcccagactgaattccaaatttgctctagtttggttacagaaaaaaaa gacttttttgccactgaaacttgagccatctgtgcctctaagaggctgagaatggaaaag tttcagataataaagagtgaagtttgcctgcaaataaagaattgagagtgtgtgcaaagc ttattttcttttatctgggcaaaaattaaaacacattccttgggacagagcctgaggtgc ctgttctgtggagaaacttctttttgagggctgtggtgaatggaagaacatacatagcaa aactgacaagatattttaaagatatataaaacacaaaggaaaaggaggttgctggtcagt cgtagcatcttacagtattggggaaaacaactgttacagtttcattgctctgagtgactg acgtgagaggaattcgctctgcagtgacgctgtctgtcactcgcctaccagctcgacgag caagagagcgggagtcagatggtccgcctcattcaccaggagccgtaactcaagctgaac tgtgaaagtggttaacactgtatcctaggccgtcttttttttcctcctcctgtttatttt ttgtttgttttatttatagtctgatttaaaacaatcagattcaagttggttaattttagt tatgtaacaacctgacgtgatggaggaaacaacctgtaaagggattgtgtctatggtttg attcacttagaaattttattttcttataacttaagtgcaataaaatgtgttttttcatgt ta PS 

TABLE 3 NGN3 protein sequence NGN3 protein Human (Homo MTPQPSGAPTVQVTRETERSFPRASEDEVTCPTSAPPSPTRTRGNCA sequence sapiens) EAEEGGCRGAPRKLRARRGGRSRPKSELALSKQRRSRRKKANDRERN NCBI Reference RMHNLNSALDALRGVLPTFPDDAKLTKIETLRFAHNYIWALTQTLRI Sequence: ADHSLYALEPPAPHCGELGSPGGSPGDWGSLYSPVSQAGSLSPAASL NP_066279.2 EERPGLLGATFSACLSPGSLAFSDFL (SEQ ID NO: 5) Mouse (Mus MAPHPLDALTIQVSPETQQPFPGASDHEVLSSNSTPPSPTLIPRDCS musculus) EAEVGDCRGTSRKLRARRGGRNRPKSELALSKQRRSRRKKANDRERN NCBI Reference RMHNLNSALDALRGVLPTFPDDAKLTKIETLRFAHNYIWALTQTLRI Sequence: ADHSFYGPEPPVPCGELGSPGGGSNGDWGSIYSPVSQAGNLSPTASL NP_033849.3 EEFPGLQVPSSPSYLLPGALVFSDFL (SEQ ID NO: 6)

TABLE 4 NGN3 nucleotide sequence NGN3 Human (Homo gataaagcgtgccaaggggcacacgacttgctgctcaggaaatccct nucleotide sapiens) gcggtctcaccgccgcgcctcgagagagagcgtgacagaggcctcgg sequence NCBI Reference accccattctctcttcttttctcctttggggctggggcaactcccag Sequence: gcgggggcgcctgcagctcagctgaacttggcgaccagaagcccgct NC_000010.10 gagctccccacggccctcgctgctcatcgctctctattcttttgcgc cggtagaaaggtaatatttggaggcctccgagggacgggcaggggaa agagggatcctctgacccagcgggggctgggaggatggctgtttttg ttttttcccacctagcctcggaatcgcggactgcgcccagtgacgga ctcaaacttacccttccctctgaccccgccgtaggatgacgcctcaa ccctcgggtgcgcccactgtccaagtgacccgtgagacggagcggtc cttccccagagcctcggaagacgaagtgacctgccccacgtccgccc cgcccagccccactcgcacacgggggaactgcgcagaggcggaagag ggaggctgccgaggggccccgaggaagctccgggcacggcgcggggg acgcagccggcctaagagcgagttggcactgagcaagcagcgacgga gtcggcgaaagaaggccaacgaccgcgagcgcaatcgaatgcacaac ctcaactcggcactggacgccctgcgcggtgtcctgcccaccttccc agacgacgcgaagctcaccaagatcgagacgctgcgcttcgcccaca actacatctgggcgctgactcaaacgctgcgcatagcggaccacagc ttgtacgcgctggagccgccggcgccgcactgcggggagctgggcag cccaggcggttcccccggggactgggggtccctctactccccagtct cccaggctggcagcctgagtcccgccgcgtcgctggaggagcgaccc gggctgctgggggccaccttttccgcctgcttgagcccaggcagtct ggctttctcagattttctgtgaaaggacctgtctgtcgctgggctgt gggtgctaagggtaagggagagggagggagccgggagccgtagaggg tggccgacggcggcggccctcaaaagcacttgttccttctgcttctc cctggctgacccctggccggcccaggcctccacgggggcggcaggct gggttcattccccggccctccgagccgcgccaacgcacgcaaccctt gctgctgcccgcgcgaagtgggcattgcaaagtgcgctcattttagg cctcctctctgccaccaccccataatctcattcaaagaatactagaa tggtagcactacccggccggagccgcccaccgtcttgggtcgcccta ccctcactca (SEQ ID NO: 7) Mouse (Mus atgcagctcagaaatccctctgggtctcatcactgcagcagtggtcg musculus) agtacctcctcggagcttttctacgacttccagacgcaatttactcc NCBI_Reference aggcgagggcgcctgcagtttagcagaacttcagagggagcagagag Sequence: gctcagctatccactgctgcttgacactgaccctatccactgctgct NC_000076.6 tgtcactgactgacctgctgctctctattcttttgagtcgggagaac taggtaacaattcggaaactccaaagggtggatgaggggcgcgcggg gtgtgtgtgggggatactctggtcccccgtgcagtgacctctaagtc agaggctggcacacacacaccttccattttttcccaaccgcaggatg gcgcctcatcccttggatgcgctcaccatccaagtgtccccagagac acaacaaccttttcccggagcctcggaccacgaagtgctcagttcca attccaccccacctagccccactctcatacctagggactgctccgaa gcagaagtgggtgactgccgagggacctcgaggaagctccgcgcccg acgcggagggcgcaacaggcccaagagcgagttggcactcagcaaac agcgaagaagccggcgcaagaaggccaatgatcgggagcgcaatcgc atgcacaacctcaactcggcgctggatgcgctgcgcggtgtcctgcc caccttcccggatgacgccaaacttacaaagatcgagaccctgcgct tcgcccacaactacatctgggcactgactcagacgctgcgcatagcg gaccacagcttctatggcccggagccccctgtgccctgtggagagct ggggagccccggaggtggctccaacggggactggggctctatctact ccccagtctcccaagcgggtaacctgagccccacggcctcattggag gaattccctggcctgcaggtgcccagctccccatcctatctgctccc gggagcactggtgttctcagacttcttgtgaagagacctgtctggct ctgggtggtgggtgctagtggaaagggaggggaccagagccgtctg gagtgggaggtagtggaggctctcaagcatctcgcctcttctggctt tcactacttggatccctagccctctcacagggcttaactaggcttct catcggtacccttgctgctgcgcacagcagacattgggggctgctct tctcttaactctcctcggtgcagccacatcaaactctcgctccaagc atttgagaatggtagcactacctagttggagactcccatacttcctg gtgagtctgccctcattcaaatctgccggcctccgaccatccatcac tttttccagggtgacctaatccagtgttgcgtcttacctcactggct cctccatccagctcttggcccatagatgatgttcgtcgtctttactg cccgctacatgcagggtttctgagcttctccattctgccttagtcca cgaaggtgatctgccttcttctgcacttttcaagtcgttacccttcc cccaagggagaccaggctgtgaaccggaaagccctagcctatggcta gagcatctctccaacttgtctcccgtgtctaaagtgtgagttgcagg gacggttcctgaagcactgtttgtctccct (SEQ ID NO: 8)

REFERENCES

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1. A method for reprogramming a somatic cell to a pancreatic β-cell, the method comprising the step of decreasing expression or activity of Fbw7 in the somatic cell.
 2. The method according to claim 1, wherein the somatic cell is a pancreatic ductal cell, a skin fibroblast or a keratinocyte.
 3. The method according to claim 1 or 2, wherein the method is carried out ex vivo.
 4. The method according to claim 1 or 2, wherein the method is carried out in vivo.
 5. The method according to claim 4, wherein the method is carried out in a subject with a metabolic disorder, such as diabetes, or in a subject at risk of developing a metabolic disorder, such as diabetes.
 6. The method according to any one of the preceding claims, wherein the method comprises contacting the somatic cell with an agent that reduces the expression or activity of Fbw7 in the somatic cell.
 7. The method according to claim 6, wherein the activity of Fbw7 is phosphorylation-mediated degradation of NGN3.
 8. The method according to claim 7, wherein the agent that reduces the expression or activity of Fbw7 inhibits phosphorylation of NGN3 by GSK3β.
 9. The method according to claim 8, wherein the agent is an inhibitor of GSK3β, for example lithium chloride, CHIR-99021 (CT99021), SB 216763, TWS119, CHIR-98014, SB 415286, tideglusib, LY2090314 or TDZD-8.
 10. The method according to any one of claims 6 to 8, wherein the agent that reduces the expression or activity of Fbw7 is a small molecule, a nucleotide sequence, a protein, a peptide, a modified peptide, an aptamer or an RNA interference (RNAi) agent.
 11. An agent that reduces the expression or activity of Fbw7 for use in a method of reprogramming a somatic cell to a pancreatic β-cell, the method comprising the step of contacting the somatic cell with said agent.
 12. An agent for use according to claim 11, wherein the somatic cell is a pancreatic ductal cell, a skin fibroblast or a keratinocyte.
 13. An agent for use according to claim 11 or 12, wherein the activity of Fbw7 is phosphorylation-mediated degradation of NGN3.
 14. An agent for use according to claim 13, wherein the agent inhibits phosphorylation of NGN3 by GSK3β.
 15. A screening method for identifying a candidate agent for reprogramming a somatic cell to a pancreatic β-cell, the method comprising determining the ability of the agent to reduce expression or activity of Fbw7, wherein an agent that reduces expression or activity of Fbw7 is likely to be able to reprogram a somatic cell to a pancreatic β-cell.
 16. The method according to claim 15, wherein the somatic cell is a pancreatic ductal cell, a skin fibroblast or a keratinocyte.
 17. The method according to claim 15 or 16, wherein the activity of Fbw7 is phosphorylation-mediated degradation of NGN3.
 18. The method according to claim 17, wherein the phosphorylation-mediated degradation of NGN3 is phosphorylation of NGN3 by GSK3β.
 19. A method for reprogramming a somatic cell to a pancreatic β-cell, the method comprising the step of decreasing phosphorylation-mediated degradation of NGN3 in the somatic cell.
 20. The method according to claim 19, wherein the somatic cell is a pancreatic ductal cell, a skin fibroblast or a keratinocyte.
 21. The method according to claim 19 or 20, wherein the method comprises the step of decreasing phosphorylation of NGN3 by GSK3β.
 22. The method according to any one of claims 19-21, wherein the method is carried out ex vivo.
 23. The method according to any one of claims 19-22, wherein the method is carried out in vivo.
 24. The method according to any of claims 19-23, wherein the method comprises the step of contacting the somatic cell with an agent that decreases the phosphorylation of NGN3 by GSK3β.
 25. The method according to claim 24, wherein the agent is an inhibitor of GSK3β, for example lithium chloride, CHIR-99021 (CT99021), SB 216763, TWS119, CHIR-98014, SB 415286, tideglusib, LY2090314 or TDZD-8.
 26. An agent that decreases the phosphorylation-mediated degradation of NGN3 for use in a method of reprogramming a somatic cell to a pancreatic β-cell, the method comprising the step of contacting the somatic cell with said agent.
 27. An agent for use according to claim 26, wherein the somatic cell is a pancreatic ductal cell, a skin fibroblast or a keratinocyte.
 28. An agent for use according to claim 26 or 27, wherein the agent inhibits phosphorylation of NGN3 by GSK3β.
 29. An agent for use according to claim 28, wherein the agent is an inhibitor of GSK3β kinase activity, such as lithium chloride, CHIR-99021 (CT99021), SB 216763, TWS119, CHIR-98014, SB 415286, tideglusib, LY2090314 or TDZD-8.
 30. A screening method for identifying a candidate agent for reprogramming a somatic cell to a pancreatic β-cell, the method comprising determining the ability of the agent to decrease phosphorylation-mediated degradation of NGN3, wherein an agent that decreases phosphorylation-mediated degradation of NGN3 is likely to be able to reprogram a somatic cell to a pancreatic β-cell.
 31. The method according to claim 30, wherein the somatic cell is a pancreatic ductal cell, a skin fibroblast or a keratinocyte.
 32. The method according to claim 30 or 31, wherein the method comprises the step of determining the ability of the agent to decrease phosphorylation of NGN3 by GSK3β, wherein an agent that decreases phosphorylation of NGN3 by GSK3β is likely to be able to reprogram a somatic cell to a pancreatic β-cell.
 33. A method of treating a subject with a metabolic disorder or a subject at risk of developing a metabolic disorder, the method comprising the step of administering to the subject pancreatic β-cells obtained by the method of any one of claim 1-10 or 19-25.
 34. A method according to claim 33, wherein the method includes: (i) the step of reprogramming a somatic cell to a pancreatic β-cell using the method of any one of claim 1-10 or 19-25; and (ii) the step of administering the β-cells obtained in step (i) to the subject.
 35. A method according to claim 33 or 34, wherein the metabolic disorder is diabetes.
 36. A pancreatic β-cell obtained by the method of any one of claim 1-10 or 19-25 for use in a method for treatment of a subject with a metabolic disorder or a subject at risk of developing a metabolic disorder, said method comprising administering said pancreatic β-cell to the subject.
 37. A pancreatic β-cell for use according to claim 36, wherein the metabolic disorder is diabetes. 